In the conditions of the Republic of Belarus there was studied the efficiency of application of granulated and extruded lupine (Lupinus) in feeding of bull claves. It was determined that after lupine extruding the quantity of sugar in it increased on 45% due to lowering of starch content. The inclusion of extruded lupine into bull calves diets made it possible to increase the digestibility of dry and organic substances, protein and nitrogen-free extractive substances. The highest average daily weight gain (842 gram) was stated at claves which were fed with mixed fodder with 15% of extruded lupine; this was on 7,5% than in a control group. In the conditions of feeding with the granulated lupine the average daily weight gain increased on 5,3% in comparison with crushed one. In course of study there was analyzed the dependence chemical composition of lupine on the processing method; blood composition of the studied animals; diets of experimental animals; digestibility of nutrient substances of forages; balance and intake of nitrogen; biochemical blood composition; changes of live weight and average daily weight gain.

In the conditions of the Republic of Belarus there was studied the formation of nonspecific protection factors of the first year foals. By a principle of conditional analogues there was generated a group of clinically healthy foals at which monthly from bulbar veins there were taken tests of blood for hematological and biochemical analysis. From the obtained data it was visible, that within the first year of foal life there were taking place the processes of formation of nonspecific protection factors of an organism which were accompanied by a gradual increasing of quantity of erythrocytes, haemoglobin, leukocytes, certain change in the diagramme, and also increasing of bactericidal and lysozyme activity of blood serum. However, at seven-month age there was marked a considerable decrease in number of erythrocytes, leukocytes, concentration of haemoglobin, bactericidal and lysozyme activity of blood serum is marked, in leukogram share reduction of segmented neutrophils, and at the same time, the content of eosinocytes and lymphocytes increased. It testified the decreasing of protective forces of an organism during this period. Consequently, the seventh month in the postnatal period of foal life proved to be the most critical.

In the conditions of the Republic of Belarus the seasonal derangements of metabolic processes at high-producing cows usually take place in the period from March till May. Analysis of efficiency of application of national preparation CMP (complex-mineral preparation -iron (15 mg/ml); iodine (6,8 mg/ml); magnesium (6,0 mg/ml); selenium (0,32 mg/ml) and vitamin preparation Trivitim (vitamin A – 30000 IU; , vitamin D – 40000 IU; vitamin E – 20 mg) for the preserving of high milking yielding capacity and prevention of metabolic processes disorders was realized. Four groups of high productive cows were formed. First group was entered 10 ml of CMP intramuscular; cows of the second group were entered 10 ml of Trivitim intramuscular; cows of the third group were entered CMP and Trivitim in the same doses; and the fourth group was a controlled one. On the 1-st, 7-th and 15-th days there was realized evaluation animal clinical state, hematologic and biochemical blood indexes (leukocytes; erythrocytes; hemoglobin; thrombocytes; ESR; total protein; albumins; urea; creatinine; glucose; calcium; phosphorous; magnesium; iron), and average daily milk yield. There was also analyzed the phagocytic activity of neutrophilic leukocytes. Research results showed that content of leukocytes and ESR in blood in all experimental groups was within the standard limits. In the first experimental group there was state the increasing of hemoglobin (from 91,4 +/- 4,38 up to 111,83 +/- 4,3 g/l (P less than0,05) ) and erythrocytes (from 4,48 +/- 0,21 up to 5,97 +/- 0,45 10E12/l) content. The same changes were stated in cows of the 2-d and 3-d experimental groups. Content of hemoglobin and erythrocyte in blood of cows which were administrated with CMP and Trivitim increased from 90,6 +/- 0,06 to 99,83 +/- 3,61 g/l and from 4,51 +/- 0,27 to 5,55 +/- 0,28 10E12/l, respectively. Analyze of biochemical indexes of cow blood showed that content of total protein, calcium, phosphorus, magnesium and iron increased. On the 15-th day there was stated the decrease of urea. Research results proved that the combined application of CMP and Trivitim was efficient and prevented subclinical disorders of metabolic processes

Investigation of influence of water improved by potassium permanganate solution on welfare and productivity of piglets was realized in the conditions of swine breeding complex in the Republic of Belarus. In the experiment there were used 2 groups of 30-day old piglets. In course of 75 days of experiment the running water of pig complex was used for watering of piglets of control group. Piglets from the experimental group were given water with addition of 0,1% solution of potassium permanganate. Analysis of obtained results showed that water with potassium permanganate promoted the increasing of natural resistance of piglets. At the same time there was stated the increasing of lysozyme and bacterial activityin blood serum. Application of improved water promoted the increasing hemoglobin on 4,8-4,5%, erythrocytes - on 2,9-4,2% in comparison with control. Content of total protein increased on 2,2-2,8% in the experimental groups, lisozyme activity - 6,9-7,0% and bacterial activity - on 5-6,9%. Application of improved water did not influence the glucose and potassium concentration in blood. In the experimental group there was stated the increased lipid concentration on 3,1-6%, cholesterol - on 4,4-6,3%, potassium level - on 2,5-4,5%, phosphorus - on 0,7-5,3%, sodium - on 1,4-3,8%. Application of water with КMnО4 increased average daily weight gain on 5,2-4,2%

Research on determination of changes of morphological and biochemical blood indexes of sheep with fasciolosis after application of the drug Klozantsid (menizol 20%, produced in the Republic of Belarus) was carried out. The research realized in the conditions of the Scientific and Research Institute of Applied Veterinary Medicine and Biotechnology of the Vitebsk State Academy of Veterinary Medicine. In course of the experiment there were used sheep of Romanovskaya breed at the age from 1,5 till 5 years which were spontaneously infected with fasciola. The experimental animals were administrated with the examined antihelminthic preparation. It was found out that after administration of the preparation there was stated the restoration of a level of erythrocytes, haemoglobin, leukocytes, enzymes, proteins and other blood indexes. Klozantsid did not render toxic influence on studied animals. | Проведены исследования по выяснению изменений морфологических и биохимических показателей крови у овец с фасциолёзной инвазией при применении препарата Клозанцид. Исследования проводили в условиях клиники и лаборатории кафедры паразитологии и инвазионных болезней, лаборатории кафедры клинической диагностики, научно-исследовательского института прикладной ветеринарной медицины и биотехнологии учреждения образования «Витебская ордена «Знак Почёта» государственная академия ветеринарной медицины». Объектом исследования являлись овцы романовской породы в возрасте от 1,5 до 5 лет, спонтанно зараженные фасциолами, которым применяли испытуемый препарат, обладающий антигельминтными свойствами. При этом выяснено, что после введения препарата происходит восстановление уровня эритроцитов, гемоглобина, лейкоцитов, ферментов, белков и других показателей. Клозанцид не оказывал токсического влияния на организм животного.

In the conditions of the Republic of Belarus there was studied the influence of herbal infusion, tincture, liquid and dry extracts of elecampane (Inula helenium) on morphological and biochemical indexes of pig blood. In course of the study it was established, that preparative forms of elecampane stimulated the formation of blood corpuscle, and also increased of the hemoglobin level in blood of pigs. Administration of elecampane preparations did not cause any authentic changes in blood leukogram of swine; all indicators were within normal limits. Elecampane preparations did not cause changes on the part of biochemical blood indexes of and did not influence the activity of enzymes. Research results showed that herbal infusion, tincture, liquid and dry extracts of elecampane did not render negative influence on swine.

In the conditions of the Republic of Belarus there was realized an experimental study of a new method treatment of calves diseased with abomasoenteritis by means of application of a preparation Ranitidin. As a result of realized research it was established that the preparation had an expressed therapeutic activity. Inclusion of Ranitidin preparation into the complex therapeutic regimen of calves sick with abomasoenteritis with the enteral feeding once in day in a dose of 0,15 g promoted the reduction of duration and decrease in severity of the disease. There were presented the results of biochemical blood indicators in trial and control groups of calves, haematologic indices in trial and control groups of calves prior to the beginning of experiment and in the end of the test.

Determination of productivity and natural resistance indexes of laying hens after application of dry multienzyme preparation Vitazim (xylanase, 3600 units/g; cellulose, 3000 units/g; beta-glucanase, 7000 units/g); as well as the specification of optimal doses the enzyme were realized in the conditions of the Republic of Belarus. Research results showed that the best indexes of egg yields of laying hens were obtained after application in the main poultry diet of enzyme preparation Vitazim in dose 500 g/t that made it possible to increase the productivity in the experimemtal group on 12%. Application of Vitazim also promoted the increasing of natural resistance level of laying hens. There was stated the positive effect of Vitazim on bactericidal activity of blood serum. Before the beginning of test this index was the level of 44,35+/-0,92 – 56,52 +/-1,63. By the 300-th day of life the bactericidal activity of blood serum in experimental group administrated Vitazim in dose 300 g/t exceeded the control idexes on 3,8%; in the second experimental group (Vitazim in dose of 500 g/h) – on 29,5%; in the third experimental group (/vitazim on dose of 700 g/h) – on 23,7%.Vitazim administration also exercised positive influence on lysozyme activity in blood serum of lying hens

For the successful decision of pressing questions of manufacture of cattle-breeding production in the field of biochemistry in whole and molecular biology in particular in-depth studies are necessary. Progress and achievements in this area are closely connected with development of new research methods. One of them is studying of proteins-lectines. Lectines, entering into structure of plant tissues, microorganisms, animal, take part in regulation of their metabolism, and also in protection against some agents of environment. On the other hand, lectines, being allocated from live objects, are the valuable biochemical reagents which use is perspective in experimental cytochemistry, in diagnostics and treatment of some animal diseases, in biotechnological processes of allocation of some difficult hydrocarbon-bearing substances. Result of the spent research was the comparative characteristic of hemagglutinin activity of phytolectines of some bean and grain crops in relation to cattle erythrocytes and precipitated with and D-yeast cellulose. Intensive precipitation with a 1-4 D-yeast cellulose was observed at its interaction with soya and wheat pectins, and less expressed - at interaction with string bean and barley pectins. It is established, that by the basic structural element of erythrocytes glycocalyx which detect soya, string beans, wheat and barley phytolectines, are monomeasures of glucose or its derivatives. The given interactions of phytolectines with a 1-4 D-yeast cellulose are presented, hemagglutination curves by results of turbodimetria are shown | Для успешного решения актуальных вопросов производства животноводческой продукции необходимы глубокие исследования в области биохимии в целом и молекулярной биологии в частности. Прогресс и достижения в этой области науки тесно связаны с развитием новых методов исследований. Одним из них является изучение белков-лектинов. Лектины, входя в структуру тканей растений, микроорганизмов, животных, принимают участие в регулировании их метаболизма, а также в защите от некоторых агентов внешней среды. С другой стороны, лектины, будучи выделенные из живых объектов, являются ценными биохимическими реагентами, использование которых перспективно в экспериментальной цитохимии, в диагностике и лечении некоторых болезней животных, в биотехнологических процессах выделения некоторых сложных углеводсодержащих веществ. Результатом проведенных исследований явилась сравнительная характеристика гемагглютинирующей активности фитолектинов некоторых бобовых и зерновых культур по отношению к эритроцитам крупного рогатого скота и преципитирующая с а 1-4 D-глюканом. Интенсивная преципитация a 1-4 D-глюкана наблюдалась при его взаимодействии с пектинами сои и пшеницы, и менее выраженная - при взаимодействии с пектинами фасоли и ячменя. Установлено, что основным структурным элементом гликокалекса эритроцитов, который детектируют фитолектины сои, фасоли, пшеницы и ячменя, являются мономеры глюкозы или ее производные. Приведены данные взаимодействия фитолектинов с а 1-4 D-глюканом, показаны кривые гемагглютинации по результатам турбодиметрии

In the conditions of the Republic of Belarus there was realized a study of age-specific features of level of erythrocytes, haemoglobin in blood, activity of aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase, as well as concentration of calcium, phosphorous, magnesium and ferrum in blood serum of suckling colts in the first 6 months of life. In the studied animals there was stated increased levels, haemoglobin and ferrum concentration in blood, but during the 6th month of life the same indexes decreased considerably. The most considerable changes оf blood serum enzymatic activities occurred at the age of 2 and 6 months. Thus, during the 2nd month of life a raise in ALAT, LDH activity, calcium and phosphorus concentration was noticed. At the age of 6 months ALAT and ASAT levels increased, and LDH activity, magnesium and phosphorus concentration decreased. During the first 6 months of life the ALP activity was on a high level and underwent no reliable changes. As the research showed, blood serum enzymatic activity and concentration of calcium, phosphorus, magnesium and ferrum depended on colt age and change at the process of growth and development of animals.