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Influence of estrogen on antibacterial and immunoglobulin secretory activities of uterine fluids from ovariectomized mares.
1994
Johnson J.U. | Oxender W.D. | Berkhoff H.A.
Effect of estrogen (E2) and progesterone (P4) on uterine antibacterial activity and immunoglobulin concentrations in mares was studied. In 2 in vitro experiments, 6 mixed-breed mares were ovariectomized, and uterine fluid and blood serum were analyzed. Antibacterial assay methods were used to determine inhibitory effects on Streptococcus zooepidemicus of uterine fluid samples collected on days 3, 5, and 8, and serum obtained on day 8 of treatment. Single radial immunodiffusion methods were used to quantify amounts of IgA and IgG in uterine fluid and serum on days 3, 5, 8, and 14 of treatment. Neither E2 nor P4 increased activity of serum and uterine fluid against S zooepidemicus. Numbers of colony-forming units per milliliter of bacteria were significantly (P < 0.01) lower in control Hanks' balanced salt solution with 1.0% gelatin (HBSSG) than in uterine fluids. Bacterial numbers were significantly (50%) greater in uterine fluids and serum than in HBSSG controls for both treatments. Both fluids, especially serum, supported significantly (P < 0.01) more growth of S zooepidemicus than did HBSSG when incubated for 0, 2, and 4 hours. These findings are in contrast to previous reports of antibacterial activity in the uterus of sexually intact mares undergoing an estrous cycle: great reduction of bacterial count in uterine fluid from mares in diestrus, and significant increases in bacterial numbers in uterine fluid or serum from mares in estrus. Treatment comparisons between serum and uterine fluid IgA and IgG concentrations were not significantly different, although overall IgA concentration in the uterus was higher than concentration in serum. The IgG concentration in uterine fluid was higher in P4- than E2-treated mares. However, IgG concentration was significantly (P < 0.01) higher in uterine fluid on day 8 in P4-treated mares than on day 3 or 5. Results of this study indicate that neither immunoglobulin concentration nor hormone treatment has a direct effec.
Afficher plus [+] Moins [-]Characterization of polypeptides synthesized and secreted by oviductal epithelial cell explants obtained from young, fertile and aged, subfertile mares.
1996
Brinsko S.P. | Ignotz G.G. | Ball B.A. | Thomas P.G.A. | Currie W.B. | Ellington J.E.
Comparison of estrogen and progesterone receptor expression in normal and tumor mammary tissues from dogs.
1995
Donnay I. | Rauis J. | Devleeschouwer N. | Wouters Ballman P. | Leclercq G. | Verstegen J.
Concentrations of estrogen (ER) and progesterone (PR) receptors were measured by radioreceptor assay in tumor (n = 319) and normal (n = 166) mammary tissue from 248 bitches. Correlations between ER and PR and between receptor expression in tumor and normal mammary tissue from the same bitches were evaluated. The influence of tumor, clinical, or hormonal variables on receptor expression also was studied. Approximately 80% of tumor and 95% of normal mammary tissue expressed detectable concentrations of ER, PR, or both. Direct correlation was found between ER and PR concentrations in normal and tumor tissues. Median ER concentrations were significantly higher (46 +/- 47 fmol/mg of cytosolic protein vs 27 +/- 24 fmol/mg of cytosolic protein; P = 0.0002) in normal than in tumor tissue. On the other hand, PR concentrations were significantly higher (57 +/- 52 fmol/mg vs 77 +/- 99 fmol/mg; P = 0.03) in tumors (especially benign tumors) than in normal tissue. Poorly differentiated malignant tumors expressed lower concentrations of receptors than did benign or well differentiated malignant tumors. The ER and PR concentrations decreased with increasing size of the lesion. Hormonal status of the bitch significantly (P < 0.05) influenced receptor expression in normal tissue: bitches in the luteal phase of the estrous cycle had higher concentrations of ER (69 +/- 62 fmol/mg) than did ovariectomized bitches (24 +/- 19 fmol/mg) or bitches in anestrus (38 +/- 45 fmol/ mg) or the follicular phase (13 +/- 7 fmol/mg). For PR, higher concentrations were observed in normal tissue during anestrus than during pseudopregnancy or in bitches treated with medroxyprogesterone acetate. Similar, but nonsignificant, variations were seen in tumor tissue except in medroxyprogesterone acetate-treated bitches in which PR concentrations were high in tumors and low in normal tissue from the same bitches.
Afficher plus [+] Moins [-]Sperm-storage tubules in the vagina of the ostrich [Struthio camelus]
1995
Bezuidenhout, A.J. | Soley, J.T. | Groenewald, H.B. (Pretoria Univ., Onderstepoort (South Africa). Dept. of Anatomy) | Burger, W.P.
Влияние ионизирующего излучения и иммобилизационного стресса на содержание прогестерона в сыворотке крови крыс
2009
Babina, T.V. | Naumov, A.D., Vitebsk State Academy of Veterinary Medicine (Belarus)
Results of studies of peculiar properties of progesterone content in blood serum of outbred female rats under the influence of intensive ionizing radiation against the background of stress stimulation realized in the conditions of the Republic of Belarus were presented. There were the following animal groups: 1. controlled rats; 2. irradiated rats; 3. stressed animals; 4. rats subjected to ionizing radiation and stress. Research results showed that concentration of progesterone in serum blood of female rats in 3 days after immobilized stress decreased on 40% in comparison with the control indexes. More intensive decreasing of hormone level took place after irradiation in dose of 1 Gy (on 62% in comparison with the controlled group). Combine activity of ionizing radiation and stress involved the decreasing of hormone concentration on 25% in comparison with the controlled group. In ten days there was the restoration of changes. Increase of progesterone concentration in blood serum took place in all tested groups. Hormone level in blood serum of irradiated rats increased on 46,04 nmol/l in comparison with results of the third day after irradiation. Hormone level in blood serum of stressed animals increased on 11,63 nmol/l, in group subjected to the combined activity of ionizing radiation and stress – on7,65nmol/l. On the thirtieth day the indexes of progesterone concentration in blood serum of irradiated rats, stressed rats and rats subjected to ionizing radiation and stress were almost similar to the control indexes
Afficher plus [+] Moins [-]Профилактика сезонных нарушений обменных процессов у высокопродуктивных коров
2009
Kovzov, V.V. | Baranovskij, A.A., Vitebsk State Academy of Veterinary Medicine (Belarus)
In the conditions of the Republic of Belarus the seasonal derangements of metabolic processes at high-producing cows usually take place in the period from March till May. Analysis of efficiency of application of national preparation CMP (complex-mineral preparation -iron (15 mg/ml); iodine (6,8 mg/ml); magnesium (6,0 mg/ml); selenium (0,32 mg/ml) and vitamin preparation Trivitim (vitamin A – 30000 IU; , vitamin D – 40000 IU; vitamin E – 20 mg) for the preserving of high milking yielding capacity and prevention of metabolic processes disorders was realized. Four groups of high productive cows were formed. First group was entered 10 ml of CMP intramuscular; cows of the second group were entered 10 ml of Trivitim intramuscular; cows of the third group were entered CMP and Trivitim in the same doses; and the fourth group was a controlled one. On the 1-st, 7-th and 15-th days there was realized evaluation animal clinical state, hematologic and biochemical blood indexes (leukocytes; erythrocytes; hemoglobin; thrombocytes; ESR; total protein; albumins; urea; creatinine; glucose; calcium; phosphorous; magnesium; iron), and average daily milk yield. There was also analyzed the phagocytic activity of neutrophilic leukocytes. Research results showed that content of leukocytes and ESR in blood in all experimental groups was within the standard limits. In the first experimental group there was state the increasing of hemoglobin (from 91,4 +/- 4,38 up to 111,83 +/- 4,3 g/l (P less than0,05) ) and erythrocytes (from 4,48 +/- 0,21 up to 5,97 +/- 0,45 10E12/l) content. The same changes were stated in cows of the 2-d and 3-d experimental groups. Content of hemoglobin and erythrocyte in blood of cows which were administrated with CMP and Trivitim increased from 90,6 +/- 0,06 to 99,83 +/- 3,61 g/l and from 4,51 +/- 0,27 to 5,55 +/- 0,28 10E12/l, respectively. Analyze of biochemical indexes of cow blood showed that content of total protein, calcium, phosphorus, magnesium and iron increased. On the 15-th day there was stated the decrease of urea. Research results proved that the combined application of CMP and Trivitim was efficient and prevented subclinical disorders of metabolic processes
Afficher plus [+] Moins [-]Взаимосвязь обмена веществ у супоросных свиноматок и полученного от них потомства
2009
Kovalenok, Yu.K. | Nikolaenko, S.A., Vitebsk State Academy of Veterinary Medicine (Belarus)
Determination of possible relations between some metabolic activity indexes of Large White pregnant sows (with various pregnancy stages) and their offspring was realized in the conditions of the Republic of Belarus. In the experimental animals there was taken blood for hematologic and biochemical tests. In peripheral blood there was analyzed the average content of hemoglobin, total number of erythrocytes, leukocytes, thrombocytes and corpuscular volume. Content of total protein, albuminolesterin, concentration total kalium and nonorganic phosphorus, magnesium, iron, zinc, activity of alanine- and aspartate transaminase were evaluated in blood serum. Blood tests were made on 84th day of pregnancy, in 30 days before farrowing, on the 2-nd lactation day and on the 2-nd and 35-th days of piglet life. Research results showed high degree of persistence of iron and zinc deficiency of pregnant sows in the conditions of modern national swine production. The obtained results proved the presence of close relationship between metabolic processes of sows and piglets that was revealed in low degree of stores supply with elements which had been in deficit for their mothers
Afficher plus [+] Moins [-]Биохимический профиль крови супоросных свиноматок
2009
Kozlov, V.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
Results of estimation of metabolic processes state in pregnant sows at different stages of sow pregnancy realized in the conditions of the Republic of Belarus were presented. Research results showed that there were changes in the analyzed indexes (total protein; albumins; total lipids; triglycerids; lactate; urea; creatinine; glucose; cholesterin; bilirubin; ALP; AAT; SGPT; amylase; gamma GTP; calcium; phosphorous; magnesium; iron) of biochemical blood profile at different stages of sow pregnancy. The most expressed divergences of the analyzed indexes were stated at pregnant sows on the 90-th day of pregnancy (10 out of 19 indexes) which were caused first of all by feeding uncertainty. It was established that level of total lipids was low during all analyzed pregnancy days, notably that the lowest indexes were registered on the 60-th day period (2,81+/-19 g/l). The lowest level of triglyceride (0,12+/-0,04 mmol/l) was stated at the same period ,too. At a later stage, this index was within the limits of physiologic standard. High level of urea in blood was registered on the 80-th day of pregnancy. Low indexes of glucose were stated on the 90-th and 100-th days of pregnancy (3,44+-/0,63 and 3,38+/-0,26 mmol/l, respectively). High level of cholesterol was stated in course of all pregnancy stages. There was low content of protein in blood in 20% of pregnant sows at the second stage of pregnancy. High indexes of creatine were registered in 80% of animals, urea – in 20% of animals. High content of bilirubin was noted in 23% of pregnant sows, high concentration of lactic acid – in 100% of animals; low content of glucose – in 38%, low level of lipids in blood – in 61%; low level of triglyceride – in 23%
Afficher plus [+] Moins [-]Использование нетрадиционных источников минерального питания в кормлении птицы
2009
Bolshakova, L.P., Vitebsk State Academy of Veterinary Medicine (Belarus)
Scientific experiments on determination of the optimal doses kizelgur for import substitution of shells in poultry diets was realized in the conditions of the Republic of Belarusby an example of 4 groups of laying hens of Khajseks korichnevyj cross. Hens of the first (controlled) group were given full ration applied at the tested poultry farm which included 5% of shells. Hens of the other three experimental groups were given complete feedstuffs with inclusion of mineral feed kizelgur: hens of the second group – in dose 2% out of dry matter mass; third group - 3%; for the fourth group - 4%. The research results showed that application of mineral feed additive kizelgur increased hen productivity in experimental groups on 2,8%; 5,9%; 7,2%, respectively. In the experimental groups there was an increasing of egg yields on 2,8%; 5,9%; 7,2%, respectively. The best indexes of poultry livability were stated in the third group (on 5,5% higher than in control). The similar tendency was stated in course of analysis of egg weight, shell thickness and calcium content of shell. Kizelgur application led to increasing of egg mass in tested groups: on 0,46% (2 group), 1,6% (3 group) and 2,4 % (4 group). At the age of 340 days shell thickness in experimental groups was on higher 3,3% (2 group), 0,7% (3 group) and 8,7 % (4 group) than in control. The analyzed additive increased the natural resistance of hens that was proved by indexes of bacterial growth-inhibitory activity of blood serum and lysozyme activity. There noted tendency of total protein increasing. Studying of immunological indexes showed that they were the most evident in the fourth experimental group
Afficher plus [+] Moins [-]Современные аспекты применения биологических стимуляторов на основе торфа
2009
Zayats, V.N. | Nadarinskaya, M.A. | Golushko, O.G. | Kvetkovskaya, A.V., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Naumova, G.V. | Makarova, N.L., The National Academy of Sciences, Minsk (Belarus). Institute for Nature Management
Determination of the positive aspects of application of a feed additive on the basis of sodium humate and malt sprouts fortified with oaky extract in diets of high-productive cows in dry period was realized in the conditions of the Republic of Belarus. Cows of black-and-white breed with average body weight 600 kg and milk-yield 6000-7000 kg were used in the experiment. Cows of experimental groups in addition to their main diet were fed with the resolved additive in doses 10-20 ml/100kg of live weight. Formula of humic additive with draft name Ehkolin-3 was developed on the basis of Ehkolin additive. Ehkolin additive in its composition contained the following compounds: organic substances; mineral substances; humic substances; melanoids; reducing agents; phenolic compounds; organic acids, and total nitrogen. Research results showed that application of the analyzed additive promoted the increasing enzyme activity in blood serum. Body weight of new born calves in experimental groups was higher than in control group. Symptoms of diseases of the digestive tract disappeared in 4-5 days in the experimental calves. Cows of the experimental groups had good colostral milk with density 1056 kg/mE3 and 55% of immunoglobulins. Impregnation capacity of in one experimental group was 50% after the first insemination, and only 33,3% and 20% in the control and the second experimental group, respectively
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