This study was designed to evaluate the effect of cyclophospaide(CY) on dietynitrosamine(DEN)-induced hepatic tumors in rats. Thirty five male of femal Sprague Dawley rats were continiously given water containing O.01% DEN for 10 weeks and then were give with CY 25mg/rat/day in water for 3, 5, 7 or 9 days. The livers of rats were removed and fixed in 10% buffered neutral formalin. The appearances of positive cells by immunohistochemical methods using proliferating cell nuclear antigen (PCNA) antibody, p53 antibody and apoptotic kit were investigated. The livers of rats given with CY were grossly brilliant, red-brown color, flexible, and thin border, and stainability of the liver cells were restored microscopically, and the vaccuolated and degenerated regions were differentiated from restored regions. These restored findings also were advanced in control group becouse of no DEN treatment but tended to be less avanced. In immunohistochemistry, positive cells to PCNA antibody appeared more numerous in control groups than that of CY treated groups. Appearance of positive cells in CY-treated group for 7 days and for 9 days were more numerous than those of CY-treated groups for 3 days and for 5 days, respectively. So these findings suggested that CY suppressed cell proliferations and effects of these action were decreased with CY-treated days. The numbers of positive cells to PCNA antibody were more prominent in hepatocelular carcinoma regions and cholangiocarcinoma regions, and then were ranked as order of large liver cell regions and normal liver cell regions. Also the numbers of the positive cells by apoptotic kit tended to be higher in hepatocelular carcinoma regions and cholangiocarcinoma regions but not uniformly in order in all regions nd were much less numbers than those of OCNA positive cells. So immunohistochemical methods using PCNA antibody together than using apoptotic kit alone when anti-carcinogen experiments. Rats with positive cells by p53 antibody were 11 of 15 rats(73.4%) in control groups and 12 of 18 rats(66.7%) in CY treated group, respectively. These positive cells appeared focally in early vacuole-occurring regions and were low in numbers.

From July of 1997 to June of 1998, total 279 raw milk samples over wkthdarwal period after mastitis treatment from dairy farms located in the provinces of Kyonggi and Choongchung were collected to test drug residues. Each sample was tested by TTC-II test and Delvotest SP. Among the total 152 raw milk samples of cow treated by beta-lactams, 32 samples(21.2%) were positive on the Delvotest and 15 samples(9.9%) showed positive on the TTC-II test. Also, from the total 37 samples treated by sulfonamides, 5 samples(13.5%) were positive on the Delvotest and 3 samples(8.1%) showed positive on the TTC-II test. For the total 55 raw milk samples of cow treated by tetracyclines, 9 samples(16.4%) were positive on the Delvotest and 5 samples(9.1%) showed positive on the TTC-II test. In addition, from the total 35 samples treated by aminoglycosides, 7 samples(20.0%) were positive on the Delvotest and 5 samples(14.3%) showed positive on the TTC-II test. Our study shows that it is possible that drugs are to be detected by the drug residues test of and individual raw milk even over the withdrawal period after mastitis treatment and the raw milk of bulk tank.

Two-day old house fly adults were exposed to six insect growth regulators, flufenoxuron, teflubenzuron, triflumuron, diflubenzuron, methoxyfenozide, tebufenozide, as a feed additive (milk + 5% sugar + chemical) in the laboratory for 6 days. The number of eggs deposited by the exposed-adults, viability of the eggs, and F1 larval development were checked. All the IGRs tested were found to have no adverse effect on the reproduction of house fly, except methoxyfenozide (210ppm). The most effective inhibitor to egg hatch was flufenoxuron, followed by teflubenzuron, triflumuron, and diflubenzuron. Exposure to flufenoxuron (over 5ppm), teflubenzuron (over 25ppm), triflumuron (over 125ppm), and diflubenzuron (over 125ppm) reduced egg hatchability to 0 to 1.3%, but lower concentrations of these IGRs were less effective (6.3 to 46.3% egg hatchability). Almost all the larvae emerged from eggs deposited by the adults exposed to diflubenzuron (62.5ppm) and teflubenzuron (12.5ppm) failed to develop into pupae, causing total mortalities of 98% and 100%, respectively. However, two IGRs, methoxyfenozide and tebufenozide, did not inhibit egg hatch and F1 larval development, except methoxyfenozide (210ppm) treatment. These results suggest that these 4 IGRs may be used in the development of autosterilization system for house fly control. However, further work is required to develop delivery systems capable of transferring an effective dose to the fly under field conditions.

This study was performed to evaluate anesthetic and cardiovascular effects of xylazine/fentanyl/azaperone and medetomidine/midazolam as preanesthetics and their combinations with antagonists in halothane-anesthetized dogs. Eight clinically healthy dogs(4.54+_2.16kg) were used at the interval of more than 14 days between experiments in turn for propionyl promazine(PP 0.3mg/kg, IM), xylazine/fentanyl/azaperone(XFA 2mg/kg, 0.0137mg/kg, 0.11mg/kg, IM), medetomidine/midazolam(MM 0.02mg/kg, 0.3mg/kg, IM), combination of XFA and their antagonists (yohimbine 0.05mg/kg, naloxon 0.0005 mg/kg, IV) and combination of MM and their antagonist(atipamezole 0.08mg/kg IM). The sedation induction times in XFA(2.56+_1.01 min) and MM(5.44+_2.07 min) groups were sighificantly better than that of PP group(10.75+_2.38 min)(p0.05). The thiopental sodium dose required for tracheal intubation in XFA(2.38+_3.38mg/kg) and MM(3.91+_3.47mg/kg)groups were significantly less than that of PP group(12.57+_2.13mg/kg)(p0.05). All time indices expressing the recovery(pedal refles recurrence time, extubation time, arousal time, standing time and walking time) were significantly shorter in the combination groups of XFA or MM with their antagonistis than in PP, XFA and MM groups(p0.05). The suppressions of cardiovascular function of XFA and MM were more than that of PP. Heart rate and cardiac output were recovered by the antagonists of XFA and MM, but mean arterial pressure were not recovered by the antagonists.

Determination of adaptation and compensation abilities of larval forms of cestodes (cysticercus and echinococci) was realized by means of evaluation of biochemical composition of their internal environment. There was realized the following estimation of biochemical composition of internal environment: Cysticercus tenuycollis from sheep - 34, Cysticercus tenuycollis from swine - 17, Echinococcus granulosus L. from swine - 23. Estimation of biochemical composition of custodies larvaceous forms was realized on the basis of the standard veterinary methods. Research results revealed that osmotic volumetric concentration of internal environment of custodies larval forms was lower than of osmolarity of blood serum. The average value of osmolarity of blood serum for both sheep and swine was 296,63 mmol/l. Research results showed presence of large quantity of specific lipo- and glycoproteins in custodies larva forms. Cysticercotic liquid administrated into suspended matter of microorganism Micrococcus lysadeicticus and leads to lyzing up to 5% of microbal bodies. It was possible to testify the presence of lyzing activity in liquid of larval forms of cestodes. The research results showed that the analyzed larval forms of cestodes had a lot of different and strong defense mechanisms. Adaptation and compensation abilities of larval forms of cestodes had nonspecific character and contained the following: physical mechanisms; humoral immunocompetent factors; and biochemical adaptation

Determination of influence of long acting antihelminthics (Aversictini boluses and boluses with albendazole) and influence of usual antihelminthics (farmatsin, alverm, fenbendavet) on first stomachanimal digestion, as well as on quantity and quality composition of microflora and microfauna in first stomach and large bowel was realized in the conditions of the Republic of Belarus. In course of the experiment there were used 6 groups of calves infested with helminths (Neoascaris vitulorum, Strongylata sp.). Research result showed that different antihelminth preparations to a greater or lesser degree rendered negative influenced on quantity and quality composition of microorganisms in first stomach and large bowel of cattle. Alverm and boluses with albendazole rendered the least influence on microflora and microfauna that could be explained by low toxicity of active substance (albendazole) of the above preparations. Aversictini boluses, fenbendavet, and farmastin changed the composition of microorganisms. It could be explained by high toxicity of preparations containing aversictini which rendered influence not only of gastro-intestinal tract but also on the whole organism. Active ingredient of mentioned above preparations (Aversektin C), being the product of microbial synthesis, rendered the antagonist action on the normal microflora of digestive tract. Negative influence of fenbendavet was caused by the chemical composition of the preparation and neurotoxic action of fenbendazol