The aim of this article was to evaluate the influence and effects of chosen bioaccumulative substances i.e. heavy metals, pesticides, and polychlorinated biphenyls (PCBs) on fish, as well as provide information on time trends and potential threat to human health. Chemical substances which pollute water may affect living organisms in two ways. First of all, large amounts of chemical substances may cause sudden death of a significant part of the population of farmed fish, without symptoms (i.e. during breakdown of factories or industrial sewage leaks). However, more frequently, chemical substances accumulate in tissues of living organisms affecting them chronically. Heavy metals, pesticides, and polychlorinated biphenyls are persistent substances with a long-lasting biodegradation process. In a water environment they usually accumulate in sediments, which makes them resistant to biodegradation processes induced by, e.g., the UV light. These substances enter the fish through direct consumption of contaminated water or by contact with skin and gills. Symptoms of intoxication with heavy metals, pesticides, and PCBs may vary and depend on the concentration and bioavailability of these substances, physicochemical parameters of water, and the fish itself.

Cadmium and zinc are often found in aquatic environment and may accumulate in living organisms. The aim of this study was to evaluate the genotoxic effect of Cd, Zn, and their binary mixture on the peripheral blood erythrocytes of Prussian carp (Carassius gibelio B.).

This work designed to monitor the hygienic quality of drinking water supply in a small commercial poultry farm and to assess the efficiency of different new disinfectant compounds against some pathogenic bacterial isolates. A total of 60 water samples was collected from both the main source and drinkers for physico-chemical and bacteriological examination. The sensitivity pattern of 40 strains of bacterial isolates to commonly used disinfectants in poultry facilities for water supply treatment was evaluated using the broth macro-dilution method. Results, the mean values of both alkalinity and total hardness were found in the highest rate of 183.0±17.6 and 345.6±7.6 mg/l compared to their values in the main water source. Furthermore, the mean value of ammonia in drinkers besides nitrite, and phosphate discovered at the highest level compared to the main water supply (1.36±0.31, 3.4±0.46, 26.3±0.78 mg/l, respectively). Both E. coli and Shigella spp. in drinkers were detected at the highest isolation rate (22.6%). Salmonella kentucky (S. kentucky: O20, 8 H I) isolates were found at the highest rate of 57.1%. Whilst the pathogenic E. coli serotyping Poly3 (O157) recorded at 66.67%, followed by E. coli O114 33.33%. Biocidal efficiency of Klorsept 25 disinfectant against investigated pathogenic bacterial isolates was 100% at a concentration of 2.0 mg/l after 180 min of exposure. Whilst the efficiency of calcium hypochlorite Ca (Ocl)2 against E. coli and S. kentucky was 100% at a concentration of 0.5 mg/l and exposure time 120 min. The susceptibility of all bacterial isolates to H2O2 disinfectant at a concentration of 5.0 % was 100% within 60 min contact time. In conclusion, the investigation of hygienic quality of water supply should be occurred periodically to ensure the safety of water source for poultry chick's health. The sensitivity of the studied pathogenic bacterial isolates is 100% to Klorsept 25 disinfectant at a concentration of 2.0 mg/l, calcium hypochlorite (Ca (Ocl)2) at 0.5 mg/l, and H2O2 at 5.0 % at exposure time does not exceed 180 min.

Fifteen samples of fish fillets were collected from different Egyptian markets to detect the residues of some heavy metals in them. The average weights of fish fillets samples were 250g. The samples were separately packed in polyethylene bag and sent to the laboratory for preparation and analysis. All equipment surfaces and utensils which used in this study were thoroughly cleaned with detergent, rinsed with water, dried and then rinsed with 2-propanol solution. The samples were stored at –2C prior to analysis. The mean levels of lead and cadmium in fish fillets collected from the Egyptian markets were 1.51  0.34 and 0.60 0.0.25 mg/kg; respectively. The concentration of lead in fish muscle were less than the permissible heavy metal limit in fish meat (2.0 mg kg) as stated by the World Health Organization, also these results were in line with the corresponding EU regulations. However the level of cadmium concentration was over than the heavy metal limits in fish meat (0.5 mg/kg) for the World Health Organization.

Objective: The goal of this study was to determine the levels of lead (Pb), cadmium (Cd), and aluminum (Al) in raw bovine milk. Milk consumption was used to calculate the estimated weekly intake (EWI), provisional tolerable weekly intake (PTWI), and target hazard quotient (THQ) for individuals. Metal distribution in dairy products and byproducts was studied as a result of artisanal processing. Material and Methods: Seventy-five raw bovine milk samples were collected from artisanal producers in Alexandria city, West Delta, Egypt, and analyzed using the atomic absorption spectrophotometer. The effect of artisanal dairy processing on metal distribution was studied. Results: The averages of Pb, Cd, and Al in milk samples were 45.06, 4.77, and 2.93 μg/l, with 13.33% and 1.33% of analyzed samples had Pb and Al levels higher than the permissible limits, respectively. The EWI values of Pb, Cd, and Al were 1.050, 0.111, and 0.068 μg/kg body weight, which contributed to about 4.20%, 1.59%, and 0.97% from the PTWI, respectively. The THQ of three metals was [J Adv Vet Anim Res 2021; 8(3.000): 454-464]

Objective: Heavy metals are serious environmental pollutants and their uptake and accumulation in the ecosystem, beyond safe limits, would cause direct consequences to food chain and ultimately to man. The present study was undertaken to ascertain the presence and levels of lead (Pb) in muscles and organs of slaughtered pigs at Nsukka agricultural zone, Nigeria. Materials and methods: From a total of 160 slaughtered pigs, liver, kidney and muscle of each pig were collected and processed for the detection of lead residue using Atomic Absorption Spectrophotometer. Results: A prevalence rate of 81.25% was recorded for Pb residue in Nsukka agricultural zone. There is a strong association (P<0.0001) between occurrence of Pb residue and the source of samples. Mean concentrations of 0.0758 mg/kg, 0.1050 mg/kg and 0.0985 mg/kg were recorded in kidney, mean concentrations of 0.1676 mg/kg, 0.1235 mg/kg and 0.1955 mg/kg were recorded in liver and mean concentrations of 0.0598 mg/kg, 0.0870 mg/kg and 0.1020 mg/kg in muscle samples in Nsukka, orba and Obollo slaughter houses respectively. There is no significant (P<0.05) difference between the mean concentrations of the Pb in the liver, kidney and muscle samples (Nsukka, Orba and Obollo) and its maximum permissible levels (MPL). Conclusion: More than half of the samples were positive for Pb and few samples that exceeded the MPL may pose human health threat to pork consumers in the study area. [J Adv Vet Anim Res 2016; 3(4.000): 392-398]

Objective: The feasibility of fermented litter as an alternative feed material for ruminant livestock is measured by organoleptic quality, fiber profile, heavy metal contamination, and the presence of worm eggs. This study aimed to examine the influence of broiler chicken litter fermentation with different fermentation lengths on organoleptic quality, and contents of cuprum (Cu), lead (Pb), worm eggs, fiber fractions including hemicellulose, cellulose, neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, and fermented litter fiber profile through analysis of scanning electron microscope-energy dispersive X-ray (SEM-EDX). Materials and Methods: This study used a complete randomized design of a unidirectional pat¬tern with four treatments and four repeats with long fermentation treatments of 0, 3, 6, and 9 weeks. Result: The results showed a real influence (p &#8804; 0.05) of fermentation length on organoleptic qual¬ity, NDF, ADF, lignin, hemicellulose, and fiber profile with SEM-EDX observations, with no presence of worm eggs and heavy metal content is still at a safe level for feed materials. Conclusion: This study concluded that the processing of broiler chicken litter with 6 weeks of fer¬mentation gave the best results on organoleptic observations, fiber profile, no presence of worm eggs, and heavy metal contamination that is safe for livestock. [J Adv Vet Anim Res 2021; 8(2.000): 312-322]

Objective: The purpose of this study was to measure the mean concentrations of heavy metals including aluminum (Al), arsenic, nickel (Ni), mercury, lead (Pb), and cadmium (Cd) and to assess the health hazards due to the exposure of cattle/human population to a distinct or the mixture of heavy metals through various sources. Materials and methods: A total of 180 samples including water sources, animal feed, and raw cows milk from rural regions in Qena, Egypt, were examined using the inductively coupled plasma emission spectrometer (ICP; iCAP 6200). Results: The data highlighted heavy metal pollution with variable concentrations among most of the investigated regions. All concentrations of Al, Ni, and Cd detected in the feeding stuff showed a strong correlation to their respective levels in milk rather than those detected in water (R2= 0.072 vs. 0.039, 0.13 vs. 0.10, and 0.46 vs. 0.014, respectively) (p < 0.05). Anisocytosis and poi¬kilocytosis with a tendency to rouleaux formation were evident, and basophilic stippling was a pathognomic indicator for heavy metal toxicity, especially Pb. Leukopenia and macrocytic anemia were shown in 50% and 65% of examined cattle, respectively. The target hazard quotients values were more than one (>1) for all heavy metals from water intake for both children and adults and Al and Cd in milk for children, and the hazard index values were indicated higher for noncarcino¬genic health hazards. The target cancer risk values predispose people in the surveyed villages to higher cancerous risks due to exposures to the mixture of heavy metal through the consumption of water and milk. Conclusion: The bioaccumulation and transmission of heavy metal mixtures from water sources and feeding material have detrimental influences on milk pollution and cattle health which seem to be a serious issue affecting public health in those rural communities. [J Adv Vet Anim Res 2020; 7(2.000): 345-359]

Three different highly consumed fish species from Al-Ahsa market, Saudi Arabia namely Spangled emporer (Lethriuns nebulosus), Red striped seabream (Pagrus major) and Black seabream (Spondyliosoma cantharus) were evaluated for their muscle contents of heavy metals (e.g., Cd and Pb) and trace elements (e.g., Cu, Zn, Fe and Mn). The possible risks associated with their human consumption were also studied. A total of 60 fresh fish samples comprising of 20 samples from each above mentioned fish were collected, and were subjected for determination of heavy metal and trace element contents by Atomic Absorption Spectrophotometry after Microwave Wet Digestion. The results showed that, accumulation patterns of the heavy metals and trace elements followed the order: Fe > Zn > Cu > Mn > Pb > Cd. There were variations among metal contents in the muscles of the three fish species; S. cantharus accumulated the highest levels of Cu, Zn and Mn, while the highest level of Fe could be detected in the muscles of P. major. The concentration of Cd and Pb remained comparable in the muscles of all three fish species. The calculated maximum daily intake (MDI) values were found as 0.0003, 0.0009, 0.0035, 0.0001, 0.0000, 0.0000 mg/day/person for Cu, Zn, Fe, Mn, Cd and Pb, respectively. Conclusively, the present study indicated that, fish muscles contain relatively less burden of heavy metals and trace elements, and no health problem can be raised from human consumption of the examined commercial fishes at Al-Ahsa market, Saudi Arabia. [J Adv Vet Anim Res 2015; 2(3.000): 271-278]

The present study intended to investigate the profile of mineral elements and metals in camel serum. For this purpose, 50 camels (Camelus dromedarius) were examined to estimate the levels of Cr, Co, Cu, Fe, Mn, Ni, Zn, B, Al, Ca, Ba, Mg, Cd, Pb, Mo, Sr, Si and V. using Inductively Coupled Argon Plasma (ICAP). On average, the mineral contents were 0.116 mg/l (Co), 0.297 mg/l (Cr), 1.817 mg/l (Cu), 13.733 mg/l (Fe), 1.521 mg/l (Mn), 0.002 mg/l (Ni), 6.775 mg/l (Zn). While mean concentrations of other metals were 0.08 mg/l (B), 42.887 mg/l (Al), 258.973 mg/l (Ca), 1.520 mg/l (Ba), 28.385 mg/l (Mg), 0.02 mg/l (Cd), 2.183 mg/l (Pb), 1.523 mg/l (Mo), 1.243 mg/l (Sr), 478.617 mg/l (Si), 0.689 mg/l (V). Although a lack of data on camel species, these findings could contribute to understanding the status of trace elements and heavy metals in camels, and any deviation of some elements from their normal values might be an indicator for exposure of these camels to pollutant circumstances.