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Comparison of different Oncorhynchus masou virus (OMV) strains by DNA restriction endonuclease cleavage analysis
1991
Gou, D.F. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Kodama, H. | Onuma, M. | Kimura, T. | Yoshimizu, M.
Cytotoxic effect of acyclovir on cultured mammalian cells to which herpesvirus thymidine kinase gene was introduced
1989
Tanabe, K. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Hiraoka, W. | Kuwabara, M. | Sato, F. | Narita, T. | Niikura, M.
Expression of the endogenous Marek's disease virus ICP4 homolog (MDV ICP4) gene is enhanced in latently infected cells by transient transfection with the recombinant MDV ICP4 gene
1995
Endoh, D. (Hokkaido Univ., Sapporo (Japan)) | Ikegawa, S. | Kon, Y. | Hayashi, M. | Sato, F.
The ICP4 homolog of Marek's disease virus (MDV ICP4) is a possible candidate for the transactivator of the early genes. We transfected MDCC-MSB-1 (MSB-1) tumor cells with plasmid including a coding region of MDV ICP4 using cationic liposome. As carriers for intranulear transport, high mobility group -1 and -2 proteins were bound to the plasmid DNA before forming liposomes. We detected transcripts from the plasmid 2 hr after transfection by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) analysis. We also detected abundant transcripts of endogenous ICP4 2-96 hr after transfection. These data suggested that expression of introduced MDV ICP4 gene enhanced the expression of endogenous MDV ICP4. On the other hand, quantitative PCR analysis for virus genome DNA indicated no significant alteration of copy number of virus genome in transfected MSB-1 cells, suggesting that reactivation of virus requires more than turning on MDV ICP4 gene
Afficher plus [+] Moins [-]Prevalence of bovine herpesvirus-1, parainfluenza-3, bovine rotavirus, bovine viral diarrhea, bovine adenovirus-7, bovine leukemia virus and bluetongue virus antibodies in cattle in Mexico
1983
Suzan, V.M. | Aguilar, R.E. (Direccion General de Sanidad Animal, Piso (Mexico)) | Onuma, M. | Murakami, Y.
Preliminary survey for antibodies against five bovine viruses [bovine leukemia virus; infectious bovine rhinotracheitis virus; adeno virus; akabane virus; parainfluenza virus] in cattle in Korea
1982
Choi, W.P. | Izawa, H. | Onuma, M. | Kodama, H. | Mikami, T. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Ohnuma, T. | Hashiguchi, Y.
