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Comparison of a radioimmunoassay (Charm II) test with high-performance liquid chromatography for detection of oxytetracycline residues in milk samples from lactating cattle.
1995
Moats W.A. | Anderson K.L. | Rushing J.E. | Wesen D.P.
A radioimmunoassay test for tetracyclines (Charm II) was compared with high-pressure liquid chromatography (HPLC) for detection of oxytetracycline (OTC) residues in milk samples from individual lactating cows. Oxytetracycline was administered by 1 of 3 routes (IV, IM, or intrauterine) to 21 lactating dairy cows. A total of 292 duplicate milk samples were collected from milkings before and through 156 hours after OTC administration. Concentration of OTC in these samples was determined by use of the Charm II test and an HPLC method with a lower limit of quantitation, approximately 2 ng of OTC/ml. Samples were also classified with respect to presence of OTC residues relative to the FDA safe concentration (less than or equal to 30 ng/ml), using the Charm II (by control point determination) and HPLC methods. There was a significant (P less than or equal to 0.05) difference between test methods in classification of milk samples with respect to presence or absence of OTC at the FDA safe concentration. A total of 48 of the 292 test results (16.4%) did not agree. Using the HPLC test results as the standard with which Charm II test results were compared, 47 false presumptive-violative test results and 1 false presumptive-nonviolative Charm II test result (a sample containing 31 ng of OTC/ml, as evaluated by HPLC) were obtained. The samples with false presumptive-violative Charm II results contained (less than or equal to 30 ng of OTC/ml, as evaluated by HPLC. In some respects, the Charm II test performed appropriately as a screening test to detect OTC residues in milk samples from individual cows. However, the tendency for the test to yield presumptive-violative test results at OTC concentrations lower than the FDA safe concentration (as evaluated by HPLC), suggests that caution should be exercised in using the test as the sole basis on which a decision is made to reject milk.
Afficher plus [+] Moins [-]Determination of sulfamethazine in chicken by HPLC
1994
Kim, J.S. | Kim, G.S. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine) | Hah, D.S. (Gyeongnam Provincial Government Institute of Health and Environment, Masan (Korea Republic))
Active metabolites in rat bile after intravenous injection of [3H]pteroylglutamic acid
1993
Shin, H.C. | Shimoda, M. | Kokue, E. (Tokyo University of Agriculture and Technology, Tokyo (Japan). Department of Veterinary Medicine)
Effects of gender on the pharmacokinetics and metabolite formation of sulfamethazine in the rabbit
1992
Yun, H.I. (Chungnam Nat'l Univ., Taejon (Korea Republic). Coll. of Veterinary Medicine) | Park, I.H. (Chungnam Nat'l Univ., Taejon (Korea Republic). Coll. of Natural Science)
