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Seroprevalence of major avian respiratory diseases in broiler and sonali chicken in selected areas of Bangladesh
2019
Zafar Ahmed Bhuiyan | Md Zulfekar Ali | Mohammad Moktader Moula | Md Akramul Bary | Nishat Arefin | Md Giasuddin | Zahed Uddin Mahmood Khan
Objective: This study was conducted to investigate different respiratory diseases in broiler and sonali birds in some selected districts of Bangladesh. Materials and Methods: We were collected a total of 460 blood samples from 46 farms with 36 broiler farms and 10 sonali farms (cross-breed) from 2015 to 2017. All the collected serum sam¬ples were tested for determining specific antibodies of avian rhinotracheitis (ART) virus, infectious laryngotracheitis (ILT) virus, infectious bronchitis (IBV) virus, and Ornithobacterium rhinotracheale (ORT) infection using commercially available enzyme-linked immunosorbent assay kits. Results: The overall seropositivity was highest in ORT (45.9%), followed by IBV (37.6%), ART (2.6%), and ILT (0.4%). Out of 360 broiler samples, highest seropositivity was recorded in ORT (43.3%) and lowest in IBV (31.4%). Surprisingly, no broiler samples were found positive for ART and ILT. In case of sonali, the seropositivity was highest in IBV (60%) and lowest in ILT (2%). With respect to types of birds and age groups, the seropositive percentage of all four pathogens was found higher in sonali than broiler. Between two age groups of sonali, the seropositive percentage of ART (12%), ORT (55%), ILT (2%), and IBV (60%) was highest at 2160 weeks of age compared to 520 weeks of age. However, based on location, the seropositive of ORT and IBV was highest in Jamalpur (63.3%) and Fulbariya and Trishal (50%) and lowest in Sreepur (16.7%) and Jamalpur (3.3%). Conclusion: The four pathogens are ubiquitous in nature for the sonali chickens, and the prev¬alence of ORT and IBV was the most prevalent viruses in the study areas. This study indicates a need for improved surveillance and characterization of ORT and ART circulating in all types of poultry in Bangladesh. [J Adv Vet Anim Res 2019; 6(4.000): 561-566]
Afficher plus [+] Moins [-]First report on the seroprevalence of avian encephalomyelitis virus antibody in Sonali (cross-bred) chickens in Bogura, Bangladesh
2021
Md Zulfekar Ali | Md Taohid Wasim Shaon | Mohammad Moktader Moula | Md Akramul Bary | Abdullah Al Momen Sabuj | Shamsul Arefin Khaled | Zafar Ahmed Bhuiyan | Md Giasuddin
Objectives: The study intended to detect the presence and distribution of avian encephalomyeli¬tis virus (AEV)-specific antibodies in Sonali (cross-bred) parent chickens regarding farm location, flock size, and age in Bogura district of Bangladesh, a Sonali chicken belt. Materials and methods: A total of 275 Sonali parent chickens blood samples were collected ran¬domly from 39 flocks during laying age with a healthy and non-vaccination history against AEV. Blood samples were collected aseptically from the wing veins of chickens using 3-ml syringes and sera were separated. Then, the sera were transferred to the laboratory by maintaining a cool chain. Indirect enzyme-linked immunosorbent assay was used to detect the specific antibodies against AEV present in the sera samples. Results: Overall, 70.18% of the chickens were found seropositive for AEV antibodies. Based on the location, the highest seropositivity was recorded in Bogura Sadar [91.30%, confidence inter¬vals (CI) 79.21%97.58%], and the lowest was in the Adomdighi sub-district (45.45%, CI 29.49% 63.08%). For flock size, AEV seropositivity was significantly (p < 0.05) higher in the large flock (82.22%, CI 72.74%89.48%). Regarding age groups, the seropositivity of AEV was significantly (p < 0.05) increased with chickens age. Higher seropositivity was noted in chickens aged >51 weeks (89.32%, CI 81.69%94.55%). Conclusion: The results indicate that AEV is circulating in the environment, and chickens were exposed to the field strain of AEV. To the best of our knowledge, this is the first report on AEV in chickens in Bangladesh. Proper vaccination and standard farm biosecurity practice could minimize AEV infection in chickens. A detailed epidemiology study, detection, and characterization of the AEV would be essential for effective AEV infection control. [J Adv Vet Anim Res 2021; 8(1.000): 78-83]
Afficher plus [+] Moins [-]Seroprevalence of strangles in horses and donkeys in Mosul city, Iraq
2023
Israa A. Al-Robaiee | Wisam S. Hassan | Maab I. Al-Farwachi
Strangles is considered an important equine contagious bacterial caused by Streptococcus equi. This study planned to evaluate the seroprevalence of anti-S. equi antibodies in horses located in Mosul city, Iraq for the first time. The sera were collected from 184 animals (156 horses and 28 donkeys), and then they were screened by using indirect ELISA (iELISA) to effectively check the seroconverted animals. The results of this study showed that the total prevalence rate of strangles in horses was 12%, (0.0% in donkeys) and the seroprevalence rate in animals less than 3 years old was 20%, while in animals of ages 3 years and more, it was 7% (P< 0.05). The seroprevalence rate in racing and horses with respiratory signs was significantly higher than ones with Draught and apparently normal conditions (P< 0.05). Any significant relationship was not found between seropositive animals and sex, and among racing horses (P< 0.05). To close, the presence of anti-S. equi antibodies in the examined horses might require more attention to reduce the incidence of the disease in horse breeding centers found in the study zone.
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