Buffaloes have high productive potential but poor manifestations of oestrus signs and silent oestrus act as a major constraint in buffaloes affecting their reproduction. There are several detection tools used for detection and confirmation of oestrus in cattle but none of the methods have succeeded to overcome the problem of silent estrus in buffaloes. In recent years proteomics approach has gained much attention to identify proteins associated with various physiological and disease conditions in saliva and cervico vaginal fluid mostly in humans and up to certain extent in cattle and buffaloes. In this study twelve pluriparous Murrah buffaloes aged 4 to 7 years with good body condition were selected and divided into group I (regular oestrus), group II (silent oestrus). Each group carries six animals and saliva was collected at proestrus (day -3), oestrus (day 0) and diestrus stage (day 7). Cervico vaginal fluid (CVF) was collected only at oestrum stage. Protein profiling of saliva were studied by SDS - PAGE analysis. The SDS-PAGE analysis showed proteins of molecular weight 150, 110, 80, 40 kDa during proestrus; 150, 120, 70 and 50 kDa during oestrus; 250, 150, 90, 50 and 37 kDa during diestrus in group I buffaloes. Similarly, proteins of molecular weight 250, 150, 100, 80 and 25 kDa during proestrus; 150, 100, 75 and 50 kDa during oestrus and 250, 100,75 and 25 kDa during diestrus were observed  in group II buffaloes. The SDS-PAGE analysis of CVF showed proteins of molecular weight of 100 and 75 kDa in group I buffaloes and 250 and 150 kDa in group II buffaloes. Oestrus specific proteins with different molecular weights were identified in terms of different bands size. However further study is warranted to find out their role during oestrus in regular and silent oestrus buffaloes for the development of specific diagnostic assay.

The study was conducted to document the incidence and nature of oestrus like signs and follicular biometry during the lunar and peri-lunar days in crossbred cattle. Crossbred cattle (n = 678) which were reported to be with oestrus like signs on lunar days (full moon day and new moon day) and peri-lunar days were subjected for the investigation. Only 60.6 per cent of the animals during the study period were in actual oestrus. The remaining animals (39.4%) were found to be in various reproductive statuses viz., pregnancy (17.7%), mid-cycle oestrum (6.9%) and without any oestrus characteristics (14.8 %). The diameter of the dominant follicle in cattle with oestrus signs during lunar days (10.4 ± 0.2 mm) was significantly (P < 0.05) smaller than cattle in actual oestrus during normal days (12.2 ± 0.5 mm). Exhibition of oestrous signs with smaller follicular diameter indicated the probable chances of lunar influence on the follicular dynamics, steroidogenesis and reproductive characters in cattle. 

The development and availability of follicles is an indicator to predict which of the follicle sizes are suitable to recover the oocytes assisted by means of ultrasonography of transvaginal oocyte retrieval (OPU). Thus, the study was done in order to characterize the follicular recruitment and distribution in response to the repeated removal of follicles, and thus to determine the availability of follicles and immature oocytes harvested repeatedlyfor two consecutive days of OPU in beef cows. Results indicated that 24-h OPU showed significantly greater numbers of medium and large follicles than small categories (P<0.05). However the 48-hr of OPU does not showed any differences of follicles categories (P>0.05). The mean total number of follicles and immature oocytes recovered were higher (P<0.05) in 24-hr OPU (13.76±1.2 and 7.38 ± 1.7) compared to 48-hr OPU (9.08 ± 1.5 and 3.54 ± 1.00) with the oocyte retrieval rate of 51.22% and 38.17%, respectively. The morphological classification indicated the 24-hr oocyte retrieval produced 62% of suitable immature oocytes that can be used for in vitro embryo production. In conclusion, the repeated removal of two consecutive days of OPU has averted the development of dominant follicle, and thus, gave an atmosphere to the subordinate follicles to continue growth relatively to an equal proportion of small, medium and large categories of follicles. Due to the reduction of follicle and recovery rate at 48-hr it is suggested that OPU be carried out later than 48 hour so that the follicle has more time to increase the diameter size.