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Bovine recombinant granulocyte-macrophage colony-stimulating factor enhancement of bovine neutrophil functions in vitro.
1990
Reddy P.G. | McVey D.S. | Chengappa M.M. | Blecha F. | Minocha H.C. | Baker P.E.
Neutrophils were purified from blood of dexamethasone-treated (0.04 mg/kg of body weight) and untreated calves. Cells were untreated (controls) or cultured in media containing 5 or 10 ng of bovine recombinant granulocyte-macrophage colony-stimulating factor (rbGM-CSF)/ ml for 10 to 12 hours before being tested for various functions. Dexamethasone treatment of calves decreased luminol-dependent chemiluminescence, decreased phagocytosis of Pasteurella multocida and several Staphylococcus spp by various degrees, and decreased antibody-dependent cell-mediated cytotoxocity against bovine herpesvirus-infected cells by 26 to 32%. The percentage phagocytosis of coagulase-positive S aureus and S intermedius was higher than that of coagulase-negative S epidermidis for neutrophils from all calves. Culture of neutrophils with rbGM-CSF significantly increased (P < 0.05) all of the aforementioned functions, compared with control neutrophils; however, rbGM-CSF-induced increases in function tended to be higher in neutrophils from dexamethasone-treated calves than in neutrophils from untreated calves.
Afficher plus [+] Moins [-]Techniques for laboratory diagnosis of cryptosporidiosis
1991
Noh, J.W. | Kang, D.W. | Jang, D.H. (Seoul National Univ., Suwon (Korea Republic). Coll. of Veterinary Medicine) | Kang, Y.B. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Spectrofluometry of sulfonamides using fluorescamine
1990
Lim, J.Y. | Han, S.N. | Lee, M.H. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Park, J.M. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
