The advantages of ventral and flank approaches for ovariohysterectomy (OHE) in terms of wound healing rate when compared to each other are still understudied. The effects of different OHE approaches on the wound healing rates of cats in Selangor, Malaysia were investigated. A total of 50 female cats were randomly included in this study. They were divided into the ventral-approach group (n=25) and the flank approach group (n=25). The wound healing rates and possible complications that could arise from the surgery in all cats were monitored daily from Day 1 until Day 7 post-surgery. Data were analysed using Independent T-test. Overall, the incision site wound healing rate in cats from the flank-approach group was found to be significantly higher (P < 0.05) than in cats from the ventral-approach group (72.55 ± 3.48 % vs. 59.91 ± 3.34 %; mean ± SEM). Complete healing of the incision site was observed in 12 % of cats from the flank-approach group as compared to 8 % of cats from the ventral-approach group. However, 8 % of cats from the ventral approach group had post-surgery complications whereas the flank-approach group had none. Results from the alternative approach may be attributed to reduced accessibility and contamination of the incision site during recovery