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Complete primary sequence of equine cartilage link protein deduced from complementary DNA.
1995
Dudhia J. | Platt D.
Investigation of the structure of equine articular cartilage link protein (LP) from individuals ranging in age from 1 to 15 years identified 3 distinct isoforms having molecular weights of 46,000, 43,000, and 41,000. The relative amounts of each of the 3 isoforms altered with age. The largest form did not change with age; however, amounts of the Mr 43,000 and 41,000 forms increased with increasing age. The results suggested that an accumulation, in the extracellular matrix of cartilage, of these 2 smaller products may have arisen from proteolytic cleavage. The complete amino acid sequence of the protein core was determined from complementary DNA products prepared by polymerase chain reaction amplification of cartilage LP mRNA. The sequence had 96% similarity with human LP and with that of other species for which the primary structure has been determined. This high degree of sequence conservation and the isoform data indicate that extracellular processing of LP occurs by similar mechanisms in various species. At the transcription level, equine chondrocytes were found to express LP as 2 abundant mRNA of 5.0 and 3.0 kb, and a smaller mRNA of 1.5 kb. Processing of the LP mRNA in horses, thus, appears to be similar to that found in other species investigated, and although multiple transcripts are present, the coding region remains unaltered and only 1 protein product is made.
Afficher plus [+] Moins [-]Studies on biological characters and plasmid profiles of Escherichia coli isolated from pigs
1990
Jeong, S.K. | Choi, W.P. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Jeong, S.C. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
Virulence-associated plasmids of Yersinia species isolated from pigs and dogs in Korea
1989
Choi, W.P. (Kyongbuk National Univ., Taegu (Korea R.). Coll. of Veterinary Medicine) | Lee, H.J. (Chungnam National Univ., Taejon (Korea R.). Coll. of Agriclture) | Jung, S.C. (Rural Development Administration, Anyang (Korea R.). Veterinary Research Institute)
Nine strains of Yersinia species isolated from pigs and dogs in Korea, comprising 5 strains of Yersinia enterocolitica, 2 strains of Y. kristensenii and each strain of Y. pseudo-tuberculosis and Y. intermedia, were examined for the presence of virulence-associated plasmids, calcium dependency and provocation of guinea pig conjunctivitis (Sereny test). Three strains of Y. enterocolitica isolated from pigs were positive in calcium dependency and harbored one plasmid of about 45 megadalton, but negative in Sereny test
Afficher plus [+] Moins [-]Activity of myeloperoxidase and leukocyte peroxidase according to ages in the hen (Dekalbwarren)
1994
Chon, S.K. | Kang, C.W. | Lee, H.I. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine)
