Pulmonary lavage samples were collected from 90- to 130-day-old calves before and 6 days after aerosol inoculation with bovine herpesvirus-1 (BHV-1) or parainfluenza-3 (PI3) virus. Alveolar lining material was separated from lavage fluids by high-speed centrifugation and phospholipids were extracted from alveolar lining material and analyzed by high-performance liquid chromatography. Phosphatidylcholine and phosphatidylethanolamine were 74.2 +/- 6.5% and 13.3 +/- 2.8%, respectively, of the total phospholipid content in the surfactant obtained from calves before virus inoculation. Other phospholipids were represented by substantially lower percentages. Infection with either of the 2 viruses caused a significant (P < 0.05) decrease in the percentage of phosphatidylcholine to 66.0 +/- 8.0% and 65.1 +/- 10.8% in the calves inoculated with BHV-1 and PI3 virus, respectively. A significant (P < 0.05) increase in the percentage of phosphatidylethanolamine to 18.1 +/- 2.2% and 17.8 +/- 4.5% developed in calves inoculated with BHV-1 and PI3 virus, respectively. Infection with BHV-1 also induced an increase (not significant) in the percentage of phosphatidylinositol from 5.5 +/- 2.8% to 7.8 +/- 2.2%. A similar, but not significant, increase in the percentage of phosphatidylinositol was also seen in the calves inoculated with PI3 virus. Less substantial changes in the percentage of other phospholipids were detected after virus infection.

In course of realized in the conditions of the Republic of Belarus studies there was analysed the chemical composition and nutrient value rape (Brassica napus) phospholipids. Research results proved that rape phospholipids contain in their composition the required quantity of energy nutrient substances (extractive substances, protein, carbohybrates) with moisture content mass - 5,1%, dry solids weight ratio - 94,9%, crude ash weight ration - 5,7%, weight fraction of fat and extractive substances - 35,5%, weight fraction of dry ethanol extract - 50,0%, weight fraiction of crude protein - 28,2%, weight fraction of crude fiber - 6,4%, totals umber of carbonhydrates including easily hydrolized - 24,4%, carotene content - 21,50 mg/kg, iron – 225,5 mg/kg, manganese – 10,4 mg/kg

Elaboration of production technology of rape (Brassica napus) phospholipids as one of components of feed additive was realized in the conditions of the Republic of Belarus. The following equipment was used for rapeseed processing: conveyer screw for seeds, conveyer for pressed seeds (seed cake), press Farraet Zralok LC preliminary, press - Farmet Zralok PL finishing, storage-capacity for oil, storage with mixing mechanism, automatic leaf filter, compressor, air vent, air filter. Rape oil was the main product of rape processing and oil seed press cake and rape phospholipides were the additional products. Quality indexes of rape phospholipids (appearance; quantity of obtained phospholipids; moisture content; dry matter content; oil and extractive matter content; total amount of carbohydrates) were analyzed. Results of phospholipids production depending on period of filtration and pressure load in the process of oil filtration were presented. Results of rape phospholipids production depending on air pressure were given. There were obtained the following optimal parameters for phospholipids production: optimal filtration time of freshly squeezed rape oil – 5-6 hours; optimal pressure load of during the filtration of raw rape oil - 2,5-3,0 atmospheres; and optimal air pressure during the filter drying - 2,5-3,0 atmospheres. The proposed production technology of rape phospholipids made it possible to produce 4,5-5,5% of phospholipids from 1 kg of rape