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Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry Texte intégral
2022
Hajrulai-Musliu, Zehra | Uzunov, Risto | Jovanov, Stefan | Kerluku, Maksud | Jankuloski, Dean | Stojkovski, Velimir | Pendovski, Lazo | Sasanya, James Jacob
Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry Texte intégral
2022
Hajrulai-Musliu, Zehra | Uzunov, Risto | Jovanov, Stefan | Kerluku, Maksud | Jankuloski, Dean | Stojkovski, Velimir | Pendovski, Lazo | Sasanya, James Jacob
Multi-class and multi-residue analyses are very complex procedures because of the physico-chemical properties of veterinary drug residues and other contaminants. The purpose of the study was to develop an analytical method for the sensitive determination of 69 analytes in bovine milk by liquid chromatography electrospray ionisation–tandem mass spectrometry. Antimicrobial, anabolic hormone, lactone, β-agonist, mycotoxin and pesticide residues were analysed in 120 raw milk samples from different dairy farms in North Macedonia. Stable isotopically labelled internal standards were used to facilitate effective quantification of the analytes. The linear regression coefficients were higher than 0.99, the limits of detection ranged from 0.0036 to 47.94 μg/L, and the limits of quantification ranged from 0.053 to 59.43 μg/L. The decision limit values ranged from 0.062 to 211.32 μg/L and the detection capability from 0.080 to 233.71 μg/L. Average recoveries of the analytes spiked in raw milk were in the range of 70.83% to 109%, intra-day coefficient of variation (CV) values from 2.41% to 22.29%, and inter-day CV values from 3.48% to 23.91%. The method was successfully applied in the testing of bovine milk samples. In five samples residues were detected. They were sulfadimethoxine (in two samples), enrofloxacin, tetracycline and oxytetracycline and were at concentrations below the EU maximum residue limit. The method is useful for routine testing for this group of chemical hazards in bovine milk.
Afficher plus [+] Moins [-]Determination of veterinary drug residues, mycotoxins, and pesticide residues in bovine milk by liquid chromatography electrospray ionisation –tandem mass spectrometry Texte intégral
2022
Hajrulai-Musliu Zehra | Uzunov Risto | Jovanov Stefan | Kerluku Maksud | Jankuloski Dean | Stojkovski Velimir | Pendovski Lazo | Sasanya James Jacob
Multi-class and multi-residue analyses are very complex procedures because of the physico-chemical properties of veterinary drug residues and other contaminants. The purpose of the study was to develop an analytical method for the sensitive determination of 69 analytes in bovine milk by liquid chromatography electrospray ionisation–tandem mass spectrometry.
Afficher plus [+] Moins [-]The impact of selected risk factors on the occurrence of highly pathogenic avian influenza in commercial poultry flocks in Poland Texte intégral
2021
Gierak, Anna | Śmietanka, Krzysztof
The impact of selected risk factors on the occurrence of highly pathogenic avian influenza in commercial poultry flocks in Poland Texte intégral
2021
Gierak, Anna | Śmietanka, Krzysztof
Introduction of highly pathogenic avian influenza virus (HPAIV) into a country and its further spread may have a devastating impact on the poultry industry and lead to serious economic consequences. Various risk factors may increase the probability of HPAI outbreak occurrence but their relative influence is often difficult to determine. The study evaluates how the densities of selected poultry species and proximity to the areas inhabited by wild birds impacted HPAI outbreak occurrence during the recently reported epidemics in Poland. The analysis was developed using these risk factors in the locations of affected and randomly chosen unaffected commercial farms. Generalised linear and non-linear models, specifically logistic regression, classification tree and random forest, were used to indicate the most relevant risk factors, to quantify their association with HPAI outbreak occurrence, and to develop a map depicting spatial risk distribution. The most important risk factors comprised the densities of turkeys, geese and ducks. The abundance of these species of poultry in an area increased the probability of HPAI occurrence, and their farming intensity in several areas of central, western, eastern and northern Poland put these areas at the highest risk. The results may improve the targeting of active surveillance, strengthen biosecurity in the areas at risk and contribute to early detection of HPAI in outbreak reoccurrences.
Afficher plus [+] Moins [-]The impact of selected risk factors on the occurrence of highly pathogenic avian influenza in commercial poultry flocks in Poland Texte intégral
2021
Gierak Anna | Śmietanka Krzysztof
Introduction of highly pathogenic avian influenza virus (HPAIV) into a country and its further spread may have a devastating impact on the poultry industry and lead to serious economic consequences. Various risk factors may increase the probability of HPAI outbreak occurrence but their relative influence is often difficult to determine. The study evaluates how the densities of selected poultry species and proximity to the areas inhabited by wild birds impacted HPAI outbreak occurrence during the recently reported epidemics in Poland.
Afficher plus [+] Moins [-]Occurrence and distribution of canine cutaneous mast cell tumour characteristics among predisposed breeds Texte intégral
2019
Smiech, Anna | Łopuszyński, Wojciech | Ślaska, Brygida | Bulak, Kamila | Jasik, Agnieszka
Occurrence and distribution of canine cutaneous mast cell tumour characteristics among predisposed breeds Texte intégral
2019
Smiech, Anna | Łopuszyński, Wojciech | Ślaska, Brygida | Bulak, Kamila | Jasik, Agnieszka
Introduction: Breed predisposition to cutaneous mast cell tumours (MCT) in a population of dogs in Poland affected by various skin tumours was assessed, and the distribution of MCT characteristics such as histological grading, sex, age, and location, in predisposed breeds was evaluated. Material and Methods: The retrospective epidemiological study included 550 dogs affected by cutaneous MCTs with a reference group of 2,557 dogs diagnosed with other skin tumours. Results: A univariable logistic regression analysis was performed to determine the odds ratios (ORs) with 95% confidence intervals. The risk of high-grade MCTs was the highest for Shar-Peis (OR: 26.394) and American Staffordshire Terriers (OR: 2.897). Boxers (OR: 6.619), Labrador Retrievers (OR: 2.630), French Bulldogs (OR: 2.050), Golden Retrievers (OR: 1.949), and American Staffordshire Terriers (OR: 2.592) were mainly affected by low-grade MCTs. The high risk of MCT was calculated to be at the age of 4–6 years for Labrador Retrievers (OR: 2.686) and 7–10 years for Boxers (OR: 2.956) and French Bulldogs (OR: 9.429). MCTs were significantly more often located on the trunk in French Bulldogs (OR: 4.680), American Staffordshire Terriers (OR: 2.520), and Labrador Retrievers (OR: 1.948). There was no statistically significant correlation between gender and the occurrence of MCTs in the breeds. Conclusions: The breed-predicated differences in the clinical course of MCTs suggest a genetic background for the tumours.
Afficher plus [+] Moins [-]Occurrence and distribution of canine cutaneous mast cell tumour characteristics among predisposed breeds Texte intégral
2019
Śmiech Anna | Łopuszyński Wojciech | Ślaska Brygida | Bulak Kamila | Jasik Agnieszka
Introduction: Breed predisposition to cutaneous mast cell tumours (MCT) in a population of dogs in Poland affected by various skin tumours was assessed, and the distribution of MCT characteristics such as histological grading, sex, age, and location, in predisposed breeds was evaluated.
Afficher plus [+] Moins [-]Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples Texte intégral
2018
Kędrak-Jabłońska, Agnieszka | Budniak, Sylwia | Szczawińska, Anna | Reksa, Monika | Krupa, Marek | Szulowski, Krzysztof
Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples Texte intégral
2018
Kędrak-Jabłońska, Agnieszka | Budniak, Sylwia | Szczawińska, Anna | Reksa, Monika | Krupa, Marek | Szulowski, Krzysztof
Introduction: The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three Bacillus anthracis genes in contaminated liver and blood samples. The goals for detection were rpoB gene as a chromosomal marker, pag gene located on plasmid pXO1, and capC gene located on plasmid pXO2. Material and Methods: Five B. anthracis strains were used for the experiments. Additionally, single strains of other species of the genus Bacillus, i.e. B. cereus, B. brevis, B. subtilis, and B. megaterium, and strains of six other species were used for evaluation of the specificity of the tests. Three SYBR Green I real-time PCRs were conducted allowing confirmation of B. anthracis in the biological samples. Results: The observation of amplification curves in real-time PCRs enabled the detection of the chromosomally encoded rpoB gene, pag gene, and capC gene of B. anthracis. The specificity of the tests was confirmed by estimation of the melting temperature of the PCR products. The sensitivity and linearity of the reactions were determined using regression coefficients. Strains of other microbial species did not reveal real-time PCR products. Conclusion: All real-time PCRs for the detection of B. anthracis in biological samples demonstrated a significant sensitivity and high specificity.
Afficher plus [+] Moins [-]Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples Texte intégral
2018
Kędrak-Jabłońska Agnieszka | Budniak Sylwia | Szczawińska Anna | Reksa Monika | Krupa Marek | Szulowski Krzysztof
Introduction: The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three Bacillus anthracis genes in contaminated liver and blood samples. The goals for detection were rpoB gene as a chromosomal marker, pag gene located on plasmid pXO1, and capC gene located on plasmid pXO2.
Afficher plus [+] Moins [-]LENGTH WEIGHT RELATIONSHIP OF NILE TILAPIA OF OREOCHROMIS NILOTICUS NILOTICUS (LINNAEUS, 1758 ) ( FAMILY : CICHLIDAE ) Texte intégral
2024
K. Karal Marx | T. Vaitheeswaran | P. Chidambaram | S. Sankarram | P. Karthiga
Length-Weight relationship was studied in Oreochromis niloticus niloticus, for a period of nine months from September 2012 to May 2013. The slope value (b) estimated for O. niloticus niloticus of both sexes was found to be 2.3133. The regression equations calculated for female was Log W = -3.4168 + 2.3133 Log L. The correlations coefficient was found to be significant (P<0.01). The significant difference between sexes of the species ‘F’ value was at 1% level. The b value differed from the ideal cube law of ‘3’ as is with the case of length-weight relationship studied in this species else where. The slope value was compared here could be very useful for comparison with the tilapia species in other geographical locations.
Afficher plus [+] Moins [-]LENGTH WEIGHT RELATIONSHIP OF NILE TILAPIA OF OREOCHROMIS NILOTICUS NILOTICUS (LINNAEUS, 1758 ) ( FAMILY : CICHLIDAE ) Texte intégral
2024
K. Karal Marx | T. Vaitheeswaran | P. Chidambaram | S. Sankarram | P. Karthiga
Length-Weight relationship was studied in Oreochromis niloticus niloticus, for a period of nine months from September 2012 to May 2013. The slope value (b) estimated for O. niloticus niloticus of both sexes was found to be 2.3133. The regression equations calculated for female was Log W = -3.4168 + 2.3133 Log L. The correlations coefficient was found to be significant (P<0.01). The significant difference between sexes of the species ‘F’ value was at 1% level. The b value differed from the ideal cube law of ‘3’ as is with the case of length-weight relationship studied in this species else where. The slope value was compared here could be very useful for comparison with the tilapia species in other geographical locations.
Afficher plus [+] Moins [-]F-wave parameters for the tibial nerve in Miniature Dachshunds with and without naturally acquired thoracolumbar intervertebral disk herniation Texte intégral
2020
Seiichi, Okuno | Orito, Kensuke
OBJECTIVE To determine values of F-wave parameters for the tibial nerve in clinically normal Miniature Dachshunds and those with thoracolumbar intervertebral disk herniation (IVDH). ANIMALS 53 Miniature Dachshunds (10 clinically normal and 43 with various clinical grades of thoracolumbar IVDH). PROCEDURES F-waves were elicited in the interosseous muscles of 1 hind limb in each dog by stimulation of the tibial nerve. F-wave parameters were measured for 32 stimuli/dog, and mean values were calculated. Linear regression was performed to assess correlations between F-wave parameters and clinical severity of IVDH. RESULTS For clinically normal dogs, mean ± SD values of shortest F-wave latency, mean F-wave conduction velocity, mean F-wave duration, and ratio of the mean F-wave amplitude to M response amplitude were 8.6 ± 0.6 milliseconds, 83.7 ± 6.1 m/s, 6.6 ± 1.5 milliseconds, and 9.8 ± 8.5%, respectively. F-wave persistence was 100%. Mean F-wave duration was positively correlated with clinical grade of IVDH. Linear regression yielded the following regression equation: F-wave duration (milliseconds) = 6.0 + 2.7 × IVDH grade. One dog with grade 2 IVDH had a mean F-wave duration shorter than that of all 5 dogs with grade 1 IVDH; 1 dog with grade 3 IVDH had a longer duration than that of all 10 dogs with grade 4 IVDH. CONCLUSIONS AND CLINICAL RELEVANCE Mean F-wave duration was correlated with the severity of inhibitory motor tract dysfunction in the spinal cord of dogs. F-wave examination may be useful for objective functional evaluation of upper motor neurons in the spinal cord.
Afficher plus [+] Moins [-]Use of crown height of the maxillary first molar tooth to approximate the age of horses Texte intégral
2018
Carmalt, James L. | Henderson, Kristen V. | Rawlinson, Jennifer E. | Waldner, Cheryl L.
OBJECTIVE To identify whether age, sex, or breed is associated with crown height of the left and right maxillary first molar tooth (M1) measured on CT images, to develop a mathematical model to determine age of horses by use of M1 crown height, and to determine the correlation between M1 crown height measured on radiographic and CT images. SAMPLE CT (n = 735) and radiographic images (35) of the heads of horses. PROCEDURES Crown height of left and right M1 was digitally measured on axial CT views. Height was measured on a lateral radiographic image when available. Linear regression analysis was used to identify factors associated with crown height. Half the data set was subsequently used to generate a regression model to predict age on the basis of M1 crown height, and the other half was used to validate accuracy of the predictions. RESULTS M1 crown height decreased with increasing age, but the rate of decrease slowed with increasing age. Height also differed by sex and breed. The model most accurately reflected age of horses < 10 years old, although age was overestimated by a mean of 0.1 years. The correlation between radiographic and CT crown height of M1 was 0.91; the mean for radiographic measurements was 2.5 mm greater than for CT measurements. CONCLUSIONS AND CLINICAL RELEVANCE M1 crown height can be used to predict age of horses. Results for CT images correlated well with those for radiographic images. Studies are needed to develop a comparable model with results for radiographic images.
Afficher plus [+] Moins [-]Racing performance of Standardbred trotting horses undergoing surgery of the carpal flexor sheath and age- and sex-matched control horses Texte intégral
2017
Carmalt, James L. | Johansson, Bengt C. | Zetterstrom, Sandra M. | McOnie, Rebecca C.
OBJECTIVE To determine factors affecting race speed in Swedish Standardbred horses undergoing surgery of the carpal flexor sheath (CFS), to investigate whether preoperative racing speed was associated with specific intraoperative findings and whether horses returned to racing, and to compare the performance of horses undergoing surgery of the CFS with that of age- and sex-matched control horses. ANIMALS 149 Swedish Standardbred trotters undergoing surgery of the CFS and 274 age- and sex-matched control horses. PROCEDURES Medical records of CFS horses were examined. Racing data for CFS and control horses were retrieved from official online records. Generalizing estimating equations were used to examine overall and presurgery racing speeds and the association of preoperative clinical and intraoperative findings with preoperative and postoperative speeds. Multivariable regression analysis was used to examine career earnings and number of career races. Kaplan-Meier survival analysis was used to compare career longevity between CFS and control horses. RESULTS CFS horses were significantly faster than control horses. The CFS horses that raced before surgery were slower as they approached the surgery date, but race speed increased after surgery. There were 124 of 137 (90.5%) CFS horses that raced after surgery. No intrathecal pathological findings were significantly associated with preoperative racing speed. Career longevity did not differ between CFS and control horses. CONCLUSIONS AND CLINICAL RELEVANCE Horses undergoing surgery of the CFS had a good prognosis to return to racing after surgery. Racing careers of horses undergoing surgery of the CFS were not significantly different from racing careers of control horses.
Afficher plus [+] Moins [-]Use of a combination of routine hematologic and biochemical test results in a logistic regression model as a diagnostic aid for the diagnosis of hypoadrenocorticism in dogs Texte intégral
2017
Borin-Crivellenti, Sofia | Garabed, Rebecca B. | Moreno-Torres, Karla I. | Wellman, Maxey L. | Gilor, Chen
OBJECTIVE To assess the discriminatory value for corticosteroid-induced alkaline phosphatase (CiALP) activity and other variables that can be measured routinely on a CBC and biochemical analysis for the diagnosis of hypoadrenocorticism in dogs. SAMPLE Medical records of 57 dogs with confirmed hypoadrenocorticism and 57 control dogs in which hypoadrenocorticism was suspected but ruled out. PROCEDURES A retrospective case-control study was conducted. Dogs were included if a CBC and complete biochemical analysis had been performed. Dogs with iatrogenic hypoadrenocorticism and dogs treated previously with glucocorticoids were excluded. Cortisol concentration for dogs with hypoadrenocorticism was ≤ 2 μg/dL both before and after ACTH administration. Cortisol concentration for control dogs was > 4 μg/dL before or after ACTH administration. RESULTS Area under the receiver operating characteristic (ROC) curve for CiALP activity was low (0.646; 95% confidence interval, 0.494 to 0.798). Area under the ROC curve for a model that combined the CiALP activity, Na-to-K ratio, eosinophil count, activity of creatine kinase, and concentrations of SUN and albumin was high (0.994; 95% confidence interval, 0.982 to 1.000). Results for this model could be used to correctly classify all dogs, except for 1 dog with hypoadrenocorticism and no electrolyte abnormalities. CONCLUSIONS AND CLINICAL RELEVANCE CiALP activity alone cannot be used as a reliable diagnostic test for hypoadrenocorticism in dogs. Combined results for CiALP activity, Na-to-K ratio, eosinophil count, creatine kinase activity, and concentrations of SUN and albumin provided an excellent means to discriminate between hypoadrenocorticism and diseases that mimic hypoadrenocorticism.
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