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Molecular phylogenetics of Newcastle disease virus isolated from chickens in 2019
2021
Faizul Fikri M. Y. | Leow, B. L. | Muhammad Redzwa, S. | Faizah Hanim M. S. | Syamsiah Aini S.
Newcastle disease (ND) is an economically important, contagious poultry viral disease reported across the globe. No recent reports on ND circulating in Malaysia. Therefore, the aim of the study is to characterize 16 Newcastle disease viruses (NDVs) isolated from chickens in Malaysia in the year of 2019. All isolates were genotypically analyzed using reverse transcription-polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Analysis of the F protein cleavage site’s deduced amino acid sequences revealed that from the Newcastle disease virus (NDV) isolates, three of them were virulent with two different motifs of 112RRQKRF117 and 12RRRKRF117 while other isolates were avirulent. Phylogenetic analysis demonstrated that three isolates were grouped in genotype VII, five in genotype I while eight in genotype II. All genotype VII isolates were clustered under sub-genotype VII.2 (VIIh and VIIi) which is the same strain causing previous outbreaks in Malaysia. Therefore, findings in this study demonstrated that there is no new introduction of NDV genotypes in Malaysia. However, farms should implement biosecurity measures at strict level as well as executing continuous monitoring and surveillance of the disease as these implementations would help them to conduct proper preventive measures and control of panzootic viruses in future.
Afficher plus [+] Moins [-]Expression of miR-29, miR-125, and miR-181 in the anterior kidneys of streptococcus-infected Nile tilapia (Oreochromis niloticus)
2018
Chiachanpongse, D. (Kasetsart University, Bangkok (Thailand). Faculty of Veterinary Medicine, Department of Veterinary Microbiology and Immunology) | Roongsitthichai, A. | Surachetpong, W.
Comparative analysis of the reproductive tract microbial communities in female dogs with and without pyometra through the 16S rRNA gene pyrosequencing
2017
Song, Y.G. (Chungnam National University, Daejeon (South Korea). College of Veterinary Medicine) | Guevarra, R.B. | Lee, J.H. | Wattanaphansak, S. | Kang, B.N. | Kim, H.B. | Song, K.H.
Detection of morulae in peripheral blood neutrophils from two dogs with Anaplasma phagocytophilum infection in Japan
2019
Fukui, Y. ((Komachi Animal Hospital, Tsukuba, Ibaraki (Japan)), (Gifu University, Gifu (Japan). United Graduate School of Veterinary Science)) | Ohkawa, S. | Inokuma, H.
