BACKGROUND: Brucellosis is one of the most common zoonosis in Middle East and Iran. OBJECTIVES: The purpose of this study was genomic detection of Brucella spp. in sero-positive dairy cattle. METHODS: We have collected 28,519 blood samples from cows during 2012-2013. Samples were screened by Slide and tube agglutination and 2-Mercaptoethanol tests. Samples with anti-Brucella antibodies titer ≥ 1:80 and ≥1:40 in tube agglutination and 2-ME tests were considered as positive respectively. Tissue samples include: lymph nodes, liver, testicle and kidney from 122 samples of slaughtered cows were collected. The Sero-positive samples were examined by a collection of specific primers for Brucella abortus, Brucella melitensis, vaccinal strains included RB51 and Rev1 using PCR tests. RESULTS: Results showed that 450 samples were positive in slide agglutination test and 447 samples had anti-brucella antibodies titer equal to or more than 1:80. So they were positive by tube agglutination test. Three hundred eighty nine samples were positive by 2- mercaptoethanol test. PCR test results showed that 46 samples (37.7%) out of 122 samples had a specific sequence of Brucella or otherwise they have an active infection with Brucella species, whereas 62.3% of samples were negative. The PCR results showed that 2 samples (4.35%) were infected by B. melitensis, 2 samples (4.35%) infected by Rev1 strain and 42 samples (91.3%) were infected by B. abortus. CONCLUSIONS: The results showed that, as we had expected, the majority of cows were infected by B. abortus. Animals who infected by B. melitensis and Rev1 strain may be a result of contact with sheep or goats. We couldn’t find Brucella genome in 76 samples (62.3%) of sero-positive cows. It may be caused by cross reaction of sera with Brucella species in tests or activation of immune system response and elimination of organism from internal organs.

BACKGROUND: Equine arteritis virus (EAV) causes respiratory disease, abortion and sometimes, neurological signs. Stallions which are permanently infected with the virus, are the constant carriers of the virus in their semen and transmit the virus to other horses through sexual contact. OBJECTIVES: The aim of this study was to evaluate EAV infection in horses in four provinces of Iran and its relationship with age, sex, and race. METHODS: Blood samples were taken from 149 horses with different sex, age and race with history or clinical signs associated with equine viral arteritis, including the manifestation of respiratory disease (fever, nasal secretion, coughing), nervous signs (ataxia, dysmetria, recumbency) and abortion. The commercial ELISA kit was used for viral antibody detection. RESULTS: From 149 sampled horses, 11 cases (7.4%) were found to be positive for EAV. Seropositive cases were recorded in Tehran (2.7%), Golestan (4.3%), Khuzestan (6.7%) and West Azerbaijan (23.8%) provinces. CONCLUSIONS: This survey confirmed the presence of EVAV in horses from four provinces of Iran with the sensitive (98.3%) and special (98.9%) test. Therefore, consideration should be given to the control and prevention programs for the spread of this virus.