Affiner votre recherche
Résultats 1-10 de 10
Failure to detect abnormal prion protein and scrapie-associated fibrils 6 wk after intracerebral inoculation of genetically susceptible sheep with scrapie agent
2002
Hamir, A.N. | Jones, Ever | Stack, M.J. | Chaplin, M.J.
Detection of the scrapie-associated protease-resistant prion protein (PrPres) in sheep brains in the early phase after intracerebral inoculation of the scrapie agent has not been documented. Fourteen 4-mo-old, genetically susceptible lambs (QQ homozygous at codon 171 of the PrP gene) were obtained for this study. Twelve lambs were inoculated intracerebrally with a brain suspension from sheep naturally affected with scrapie, and 2 served as uninoculated controls. Two inoculated animals were euthanized at each of 6 times postinoculation (1 h to 6 wk), and their brains were collected for histopathological study, for detection of PrPres by the Western blot technique and an immunohistochemical (IHC) method, and for the detection of scrapie-associated fibrils (SAF) by negatively stained electron microscopy (EM). Microscopic lesions associated with introduction of the inoculum were seen in the brains of inoculated animals at all 6 times. However, both the Western blot and IHC techniques did not detect PrPres after the initial 3 d postinoculation, nor did EM detect SAF in any of the samples. From these findings, it is presumed that until host amplification has occurred, the concentration of PrPres in inoculum is insufficient for detection by currently available techniques.
Afficher plus [+] Moins [-]The Kumm isolate of Ehrlichia ruminantium: In vitro isolation, propagation and characterization
2002
Zweygarth, E. | Josemans, A.I. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepooort Veterinary Inst.) | Van Strijp, M.F. | Van Heerden, H. | Allsopp, M.T.E.P. | Allsopp, B.A.
Teratogenicity of a mutagenised Rift Valley fever virus (MVP 12) in sheep
2002
Hunter, P. (Onderstepoort Biological Products Ltd. (South Africa)) | Erasmus, B.J. | Vorster, J.H.
An overview of the eradication of Brucella melitensis from KwaZulu-Natal [South Africa]
2002
Emslie, F.R. | Nel, J.R.
Continuous exposure to an aversive mixture as a means of maintaining aversion to vermeerbos (Geigeria ornativa O. Hoffm.) in the presence of non-averted sheep
2002
Snyman, L.D. | Schultz, R.A. | Kellerman, T.S. | Labuschagne, L. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.)
Effects of the scab mite Psoroptes ovis on the haematology and live mass of Merino and Dorper sheep
2002
Meintjes, T. | Fourie, L.J. | Horak, I.G. (Free State Univ., Bloemfontein (South Africa). Zoology and Entomology Dept.)
On-host ecology and off-host survival of the sheep scab mite Psoroptes ovis
2002
Meintjes, T. | Fourie, L.J. | Horak, I.G. (Free State Univ., Bloemfontein (South Africa). Zoology and Entomology Dept.)
Amphistome infection of goats farmed under resource-poor conditions in South Africa
2002
Vatta, A.F. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) | Krecek, R.C.
Freezing of sheep faeces invalidates Haemonchus contortus faecal egg counts by the McMaster technique
2002
Van Wyk, J.A. (Pretoria Univ., Onderstepoort (South Africa). Veterinary Tropical Diseases Dept.) | Van Wyk, L.
Strategic control of gastrointestinal nematodes of sheep in the highlands of central Kenya
2002
Maingi, N. (Nairobi Univ. (Kenya). Veterinary Pathology, Microbiology and Parasitology Dept.) | Otieno, R.O. | Gichohi, V.M. | Weda, E.H.