This study was conducted to determine the optimum storage temperature for Rinderpest vaccine prepared on vero cells to know the shelf life of the vaccine. The vials were randomly selected from one batch of the vaccine, titrated and stored at minus 20 degree centigrade (Freezer), 4 degree centigrade (refrigerator) and room temperature. The titre was found to be 105.1 per ml. The vials stored at minus 20 degree centigrade & 4 degree centigrade were subjected to titration after an interval of six months for 3 and 2 years respectively. The vials stored at room temperature were tested after 4 weeks, 8 weeks and 12 weeks. Titration results indicated that the titre of the vaccine vials stored at room temperature decreased by 100.9 101.2 and 101.6 after storage time of 4 weeks, 8 weeks and 12 weeks respectively. The vials stored at 4 degree centigrade maintained their titre for a period of six months but after that the loss in titre was 100.4, 101.0, and 102.4 after storage time of one, one and half and two 2 years respectively. The vaccine vials stored at minus 20 degree centigrade maintained their original titres (initial titre of the vaccine) even after the storage for three years. It is concluded that vero cell adapted Rinderpest virus vaccine can be stored at 4 degree centigrade for a period of six months, however, at 20 degree centigrade it can be stored for three years without any adverse effect on titre.