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Investigation of a listeriosis epizootic in sheep in New York state.
1997
Wiedmann M. | Arvik T. | Bruce J.L. | Neubauer J. | Piero F. del | Smith M.C. | Hurley J. | Mohammed H.O. | Batt C.A.
Biochemical analysis of normal articular cartilage in horses.
1990
Vachon A.M. | Keeley F.W. | McIlwraith C.W. | Chapman P.
Articular cartilage specimens from the distal articular surface of 32 radiocarpal bones from 24 2- to 5-year-old horses were analyzed. The total collagen content was determined on the basis of the 4-hydroxyproline content, using a colorimetric method. A method for estimating the proportions of types-I and -II collagen by measuringspectrophotometric densities of specific cyanogen bromide peptide bands from mixtures of types-I and -II collagen on sodium dodecyl sulfate-polyacrylamide gels was used. The cyanogen bromide peptides representative of each collagen types-I and -II were identified. The peptide ratios were then computed for each of several standards of type-I and -II mixtures. A standard curve was derived from the correlation between these ratios and the corresponding proportions of type-II collagen in standard mixtures. Galactosamine and glucosamine content (hexosamines) were measured by ion chromatography. Thegalactosamine-to-glucosamine ratio, chondroitin sulfate and keratan sulfate values, and total glycosaminoglycan content were derived from the measured hexosamine content. The total collagen content averaged 556 mg/g (55.6 mg/100 mg) of tissue (dry weight, [dw]). Type-II collagen was the major collagen type in normal articular cartilage specimens. The ratio of the area under the alpha 1 (II)CB10 peak to the area under the alpha 1 (I)CB 7,8 + alpha 1 (II)CB11 peak was a second-order polynomial function of the proportion of type-II collagen in the specimens. The mean galactosamine and glucosamine content were 20.6 mg/g and 7.9 mg/g (dw), respectively. The meangalactosamine-to-glucosamine ratio was 3.74 +/- 0.62. Chondroitin sulfate values, keratan sulfate values, and total glycosaminoglycan content were 53.3 +/- 4.9 mg/g, 19.9 +/- 3.6 mg/g, and 73.2 +/- 7.9 mg/g (dw), respectively. There was no significant correlation between the age of the horses and any of the chemical values (P>0.1). The biochemical composition of articular cartilage in the horse is similar to that of other species.
Afficher plus [+] Moins [-]Quantification of phenylbutazone in equine sera by use of high-performance liquid chromatography with a nonevaporative extraction technique.
1996
Peck K.E. | Ray A.C. | Manuel G. | Rao M.M. | Foos J.
Lymphoscintigraphy in healthy dogs and dogs with experimentally created thoracic duct abnormalities.
1992
Hodges C.C. | Fossum T.W. | Komkov A. | Hightower D.
Lymphoscintigraphic evaluation of the thoracic duct (TD) was performed in 10 healthy and 12 dogs with experimentally created TD abnormalities (6 dogs with TD lacerations and 6 dogs with cranial vena ligations). Complete imaging took 4 hours and caused no adverse effects or complications. Lymphoscintigraphy of healthy dogs failed to image the TD; however, background activity in the abdomen and thorax, and radioactivity in the kidneys, bladder, liver, and heart were noticed. Lacerations and transections of the TD were experimentally created, in 6 dogs to ascertain whether TD rupture could be detected with lymphoscintigraphy. Lymphoscintigraphy was performed within 48 hours of creating the TD defect. There was no significant difference in the scintigraphic pattern of healthy dogs and those with experimentally created TD defects. Ligation of the cranial vena cava was performed in 6 dogs; 3 dogs developed chylothorax. In those 3 dogs, diffuse radioactivity was imaged in the thorax and was compatible with thoracic lymphangiectasia. In one of these dogs, linear activity consistent with the TD and localized regions of radioactivity cranial to the heart (compatible with the mediastina lymph nodes) were noticed. Lymphoscintigraphic findings in these dogs correlated with lymphangiographic findings.
Afficher plus [+] Moins [-]Comparative study of leptospiral strains ictero No. 1 and RGA by restriction endonuclease DNA analysis.
1988
Hata K. | Yamaguchi T. | Ono E. | Yanagawa R.
Identification of Staphylococcus hyicus subsp hyicus of swine and poultry origin by API STAPH-IDENT system.
1994
Park C.K.
Development of an enzyme immunoassay for determination of steroid hormones to improve the reproductive efficiency of domestic animals.
1993
Choi H.S. | Kang B.K.
Restriction endonuclease analysis of a porcine isolate of bovine herpesvirus type I.
1994
Varady E. | Tuboly T. | Derbyshire J.B.
Somatic cell count thresholds in composite and quarter milk samples as indicator of bovine intramammary infection status Texte intégral
2017
Petzer, Inge-Marié | Karzis, Joanne | Donkin, Edward F. | Webb, Edward C. | Etter, Eric
Somatic cell count thresholds in composite and quarter milk samples as indicator of bovine intramammary infection status Texte intégral
2017
Petzer, Inge-Marié | Karzis, Joanne | Donkin, Edward F. | Webb, Edward C. | Etter, Eric
The objective of the study was to establish an operational somatic cell count (SCC) threshold to predict the presence of intramammary infection (IMI) in composite milk samples and compare findings with those in quarter milk samples. South African dairy producers now preferred composite milk samples for herd udder health analysis because of increasing cow numbers, convenience of sampling and lower cost. A retrospective study was conducted on 345 461 composite and 89 638 quarter milk samples from South African herds. Variance estimates for the proportion of quarter samples testing positive were adjusted to account for the lack of their independence within individual cows. The IMI at SCC thresholds of 150 000 cells/mL and 200 000 cells/mL differed only by 3.26% in composite milk samples. Youden's index indicated the optimum SCC thresholds for composite and quarter milk samples as 150 000 cells/mL and 200 000 cells/mL, respectively. At 150 000 cells/mL, sensitivity (95% confidence intervals [CI]) in composite milk samples was 65.3% (64.0%, 66.6%) and specificity was 66.8% (65.7%, 67.9%); and in quarter milk samples, sensitivity at 200 000 cells/ mL was 70.8% (69.5%, 72.0%) and specificity was 63.6% (62.4%, 64.8%). The likelihood of infection for udders and quarters, respectively, was 1.034 and 1.327 at an SCC threshold of 150 000 cells/mL and 0.864 cells/mL and 1.177 cells/mL at 200 000 cells/mL. The area under the curve of the receiver operating characteristics graph was 0.7084 and 0.7277 for composite and quarter samples, respectively, indicating that the SCC test could be considered as a good indicator of IMI in both sample types. (Résumé d'auteur)
Afficher plus [+] Moins [-]Somatic cell count thresholds in composite and quarter milk samples as indicator of bovine intramammary infection status Texte intégral
2017
Inge-Marié Petzer | Joanne Karzis | Edward F. Donkin | Edward C. Webb | Eric M.C. Etter
The objective of the study was to establish an operational somatic cell count (SCC) threshold to predict the presence of intramammary infection (IMI) in composite milk samples and compare findings with those in quarter milk samples. South African dairy producers now preferred composite milk samples for herd udder health analysis because of increasing cow numbers, convenience of sampling and lower cost. A retrospective study was conducted on 345 461 composite and 89 638 quarter milk samples from South African herds. Variance estimates for the proportion of quarter samples testing positive were adjusted to account for the lack of their independence within individual cows. The IMI at SCC thresholds of 150 000 cells/mL and 200 000 cells/mL differed only by 3.26% in composite milk samples. Youden’s index indicated the optimum SCC thresholds for composite and quarter milk samples as 150 000 cells/mL and 200 000 cells/mL, respectively. At 150 000 cells/mL, sensitivity (95% confidence intervals [CI]) in composite milk samples was 65.3% (64.0%, 66.6%) and specificity was 66.8% (65.7%, 67.9%); and in quarter milk samples, sensitivity at 200 000 cells/ mL was 70.8% (69.5%, 72.0%) and specificity was 63.6% (62.4%, 64.8%). The likelihood of infection for udders and quarters, respectively, was 1.034 and 1.327 at an SCC threshold of 150 000 cells/mL and 0.864 cells/mL and 1.177 cells/mL at 200 000 cells/mL. The area under the curve of the receiver operating characteristics graph was 0.7084 and 0.7277 for composite and quarter samples, respectively, indicating that the SCC test could be considered as a good indicator of IMI in both sample types.
Afficher plus [+] Moins [-]Somatic cell count thresholds in composite and quarter milk samples as indicator of bovine intramammary infection status Texte intégral
2017
Petzer, Inge-Marié(University of Pretoria Department of Production Animal Studies) | Karzis, Joanne(University of Pretoria Department of Production Animal Studies) | Donkin, Edward F.(University of Pretoria Department of Animal and Wildlife Sciences) | Webb, Edward C.(University of Pretoria Department of Animal and Wildlife Sciences) | Etter, Eric M.C.(University of Pretoria Department of Production Animal Studies ,French Agricultural Research Centre for International Development Department Environment and Societies)
The objective of the study was to establish an operational somatic cell count (SCC) threshold to predict the presence of intramammary infection (IMI) in composite milk samples and compare findings with those in quarter milk samples. South African dairy producers now preferred composite milk samples for herd udder health analysis because of increasing cow numbers, convenience of sampling and lower cost. A retrospective study was conducted on 345 461 composite and 89 638 quarter milk samples from South African herds. Variance estimates for the proportion of quarter samples testing positive were adjusted to account for the lack of their independence within individual cows. The IMI at SCC thresholds of 150 000 cells/mL and 200 000 cells/mL differed only by 3.26% in composite milk samples. Youden's index indicated the optimum SCC thresholds for composite and quarter milk samples as 150 000 cells/mL and 200 000 cells/mL, respectively. At 150 000 cells/mL, sensitivity (95% confidence intervals [CI]) in composite milk samples was 65.3% (64.0%, 66.6%) and specificity was 66.8% (65.7%, 67.9%); and in quarter milk samples, sensitivity at 200 000 cells/mL was 70.8% (69.5%, 72.0%) and specificity was 63.6% (62.4%, 64.8%). The likelihood of infection for udders and quarters, respectively, was 1.034 and 1.327 at an SCC threshold of 150 000 cells/mL and 0.864 cells/mL and 1.177 cells/mL at 200 000 cells/mL. The area under the curve of the receiver operating characteristics graph was 0.7084 and 0.7277 for composite and quarter samples, respectively, indicating that the SCC test could be considered as a good indicator of IMI in both sample types.
Afficher plus [+] Moins [-]Characterization of two monoclonal antibodies which recognize different subpopulations of chicken T lymphocytes
1990
Kondo, T. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Hattori, M. | Kodama, H. | Onuma, M. | Mikami, T.
