The aim of these experiments was to investigate the effects of bovine oviduct epithelial cells(OEC) derived from different segments to bind sperm binding and maintain their motility in vitro. In experiment 1, the number of sperm attached to OEC derived from isthmus or ampulla, the motility of unattached sperm during co-culture and fertilizing ability were assessed. In experiment 2, heparin treated sperm 9hsp) or no treated sperm (nsp) were used to evaluate OEC binding ability ofcapacitated sperm. In experiment 1, regardless ofthier origin, approximately 65% of the sperm were attached to OEC within 2h. From 6h of co-culture, the numbers of unattached sperm on ampullary OEC were significantly higher than those on isthmic OEC (p0.005). From 12h of co-culture, the motility of unattached sperm on isthmic OEC were significantly higher than those on ampullary OEC(p0.05). The cleavage rate of oocytes inseminated on OEC derived from isthmic segment was also significantly higher than those from ampullary segment (p0.01). In experiment 2, the numbers of unattached hsp on OEC were significantly higher than those of controls(p0.01), between 2~24h examination. From 12h of co-culture, the motility of unattached nsp were sighificantly greater than those of hsp(p0.01). These results show that bovine OEC derived from the isthmus play more important role(s) for sperm binding, maintaining motility and fertilization in vitro than those from the ampulla, and heparin induced capacitation may change sperm binding ability on OEC in vitro.

Study of the regeneration processes and raising the efficiency of rape (Brassica napus) organs and tissues cultivation in vitro methods, as well as the achievement of a stable yield of proper regenerants were realized in the conditions of the Republic of Belarus. The obtained data specified the possibility of generation of rape finished regenerants in vitro culture without additional passages and transfers by means of effectively selected concentrations of phytohormones and biologically active substances, which made it possible to realize principles of totipotency and polarity of crop vegetative organs. On the explant part which was submerged into culture medium there was formed a root, and on opposite one - the morphogen structures. The possibility of obtaining rape regenerants from hypocotyls segments by straight regeneration was studied. Application of the analyzed substances considerably simplified and reduced the price of process of full regenerants formation, and made it possible to receive a wide spectrum of various forms that was important for selection process due to the opportunity to select valuable (in protein content) samples for the further studies and their inclusion into production process

In the conditions of the Republic of Belarus there was realized creation and evaluation of growth activity of serum and albuminous nutritional media. In the experiment there were used of 6 pathogenic strains: Leptospira pomona; Leptospira tarassovi; Leptospira grippothyphosa; Leptospira icterrohaemorragia; Leptospira conicola; Leptospira sejroe. For the Leptospira cultivation there were used the following media: serum-supplemented vitaminized medium on the basis of buck serum; serum-supplemented medium with a growth factor; serum-supplemented vitaminized medium with antlbody biosynthesis growth factor; semisynthetic Russel's medium in modification of V.I. Sitkov. Research results showed that the albuminous nutritional medium provided an intensive accumulation of Leptospira of all tested serogroups of Leptospira, but was suitable only for single bacterial inoculation. For the secondary hatching the albuminous media was unsuitable as it lowered the antigenic activity of Leptospira.