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Comparison of a radioimmunoassay (Charm II) test with high-performance liquid chromatography for detection of oxytetracycline residues in milk samples from lactating cattle.
1995
Moats W.A. | Anderson K.L. | Rushing J.E. | Wesen D.P.
A radioimmunoassay test for tetracyclines (Charm II) was compared with high-pressure liquid chromatography (HPLC) for detection of oxytetracycline (OTC) residues in milk samples from individual lactating cows. Oxytetracycline was administered by 1 of 3 routes (IV, IM, or intrauterine) to 21 lactating dairy cows. A total of 292 duplicate milk samples were collected from milkings before and through 156 hours after OTC administration. Concentration of OTC in these samples was determined by use of the Charm II test and an HPLC method with a lower limit of quantitation, approximately 2 ng of OTC/ml. Samples were also classified with respect to presence of OTC residues relative to the FDA safe concentration (less than or equal to 30 ng/ml), using the Charm II (by control point determination) and HPLC methods. There was a significant (P less than or equal to 0.05) difference between test methods in classification of milk samples with respect to presence or absence of OTC at the FDA safe concentration. A total of 48 of the 292 test results (16.4%) did not agree. Using the HPLC test results as the standard with which Charm II test results were compared, 47 false presumptive-violative test results and 1 false presumptive-nonviolative Charm II test result (a sample containing 31 ng of OTC/ml, as evaluated by HPLC) were obtained. The samples with false presumptive-violative Charm II results contained (less than or equal to 30 ng of OTC/ml, as evaluated by HPLC. In some respects, the Charm II test performed appropriately as a screening test to detect OTC residues in milk samples from individual cows. However, the tendency for the test to yield presumptive-violative test results at OTC concentrations lower than the FDA safe concentration (as evaluated by HPLC), suggests that caution should be exercised in using the test as the sole basis on which a decision is made to reject milk.
Afficher plus [+] Moins [-]Studies on the early diagnosis of pregnancy of dairy cows by EIA-kit of progesterone in milk.
1990
Kim M.K. | Shin H.J. | Lee M.H. | Lee M.H. | Kim S.K.
Studies on the improvement of reproductive efficiency in Korean native cows - Plasma progesterone concentrations for monitoring ovarian activity in the postpartum period
1990
Choi, H.S. | Kang, B.K. | Son, C.H. (Chonnam National University, Kwangju (Korea Republic). College of Veterinary Medicine) | Suh, G.H. (National Animal Breeding Institute, Namwon (Korea Republic). Namwon Branch)
The effects of exogenous insulin-like growth factor-I on broiler chicken growth
1994
Lee, H.I. | Kang, C.W. | Chon, S.K. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine) | Lee, M.J. (Iri Agricultural and Technical College, Iri (Korea Republic)) | Lee, D.Y. (Chonbuk National University, Chonju (Korea Republic). Medical School) | Kim, Y.A. (Woosuk University, Wanju (Korea Republic). Faculty of Pharmacy)
Characteristics and application of monoclonal antibody to progesterone - (1) - Production of monoclonal antibody to progesterone
1990
Kang, C.B. | Kim, Y.H. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)
