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A study of the bone marrow derived mesenchymal stromal cells in rats – proliferation and immunophynotypic markers
2018
Mauida, F. Hasoon, | Buktiar, Nader | Majed, H. Mohammed
Mesenchymal stem cells derived from bone marrow (BM-MSCs) havethe ability to differentiate into multiple cell lineages. Although the cultivation of these cells has led to a number of characterisation studies, some significant morphological and immunohistochemical properties are still lacking. In this study, isolation of BM-MSCs, morphological features, cell viability, immunophenotypic properties and cryopreservation of BM-MSCs wereexamined in detail. The results demonstrate that the cells isolated from BM-MSCs were plastic adherent and had fibroblastic spindle shape after three passages and get confluent monolayer cells 70-80% after 4-7 days post-subculture. Based on the cell viability analysis, the BM-MSCs showed an increase in cell viability starting from passage 1 until passage 10. Immunophenotypic analysis demonstrated that BM-MSCs were positivefor CD44 and CD105 and negative for CD34. Functional analysis of cryopreservation of BM-MSCs from P6 after 6 months expressed good proliferation rate and cell viability.
Afficher plus [+] Moins [-]Evaluation of neem leaf (Azadirachta indica) product for worm control on goats
2013
Premaalatha B. | Zary Shariman Y. | Norhafiza N. H. | Nurulaini R. | Chandrawathani P. | Nor Andilla I. | Rahimah H. | Wahab A. R.
This study was conducted with the aim of investigating the
effectiveness of Neem leaf extracts, Azaridachta indica, an indigenous medicinal plant, against helminths of goats. Two extracts were prepared with distilled water using two different techniques; that is, the Neem leaves Water Extract (NLWE) and decoction of Neem
leaves(NLD). This study was carried out using 20 goats from a government farm in Perak. The two extracts showed variable degrees of efficacy in naturally infected goats, as measured by faecal egg count. Further evaluation is required to establish the efficacy.
Afficher plus [+] Moins [-]Corona Discharge Therapy for a Malaysian elephant (Elephas maximus hirsutus)
2012
Vellayan S. | Yap, M. T.
Corona Discharge Therapy,an evidence-based therapeutic modality, was used on a 40-year-old female Malaysian elephant. The elephant was on systemic therapy for her wounds for 5 days. All therapies were then stopped for 4 days. Corona Discharge Therapy was subsequently applied on both the hind limb joints for 3 sessions. The non-invasive
treatment was made with a multi-patented, electronic therapeutic device called VET Sonotron without the concurrent use of drugs. Corona discharge beam (CDB) of the device was used to relieve the joint pain and inflammation. The skin surface of the elephant joints was scanned with the CDB emanating from a discharge electrode to
which was applied periodic bursts of radio frequency at 0.43 mHz, pulsed at 1 kHz of sound frequency. This therapy showed remarkable anti-inflammatory effects.
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