Production examination of efficiency of concentrated vaccine against swine erysipelas from matrix Konev of trial series, control of its immunizing power and production experiments of biological preparation were realized in the conditions of the Republic of Belarus. Research results showed that the presented vaccine had more expressed immunogenic properties in comparison with its production analogue and provided piglets protection against infection. There was analyzed the immunological efficiency of the vaccine: determination of quantity of erythrocytes; leukocytes; content of hemoglobin, level of lysozyme activity, bacterial growth-inhibitory activity, blood serum, phagocytic activity, neutrophilic leukocytes, content of total protein and protein fractions, titre of erysipelas antibodies, and preventive properties of blood serum. Efficiency of biological preparation was determined on the basis of analysis of disease incidence rate, loss of immunized swine caused by swine erysipelas

The realized studies of an experimental series of inactivated vaccine showed that the developed biological preparation made it possible to achieve high preventive efficiency in the conditions of cows heavy with calves for the creation in newborn calves the expressing immunity after milk feeding in the first hours of their life. As a result realized in the conditions of the Republic of Belarus production experiment of emulsified vaccine against rotavirus infection and colibacillosis of cattle in animal breeding farms of different regions of the Republic the prophylactic efficacy of a developed vaccine made up 97,9-98,9%. Research results showed that the developed inactivated vaccine caused at the immunizated animals the formation of specific antibodies at a higher level in comparison with other analyzed national and foreign analogue preparations.

A description of a method of chemical inactivation of Aujeszky's disease virus by teotropin was studied in the conditions of the Republic of Belarus. The developed inactivated viral suspension was tested for its antigenic activity. On the basis of realized experiments there were determined the optimal parameters of inactivation of Aujeszky's disease virus y means of 0,1% solution of teotropin under temperature of 37 deg C, pH 7,8 and duration of exposition of 24 hours. There were studied indexes of antigenic activity of inactivated virus

Immunomorphological changes in young hens vaccinated with an associated inactivated emulsion vaccine against bursal disease, infectious bronchitis and Newcasle disease was realized in the conditions of the Republic of Belarus. Replacement young hens of 130-158 days old were divided into 2 groups with 200 head in each according the analogue principle. At the age of 130 days old the first experimental group of chickens was immunized against bursal disease, infectious bronchitis and Newcasle disease with a liquid inactive emulsion vaccine (produced in the republic of Belarus) in dose of 0,5 ml intramuscularly. In course of the experiment chickens from each group were killed on the third, seventh, fourteenth, twenty-first and twenty-eighth days. For the analysis of immunomorphological reaction there were tested tissue pieces from the places of vaccine administration, as well as thymus, bursa of Fabritius, lien, cecal tonsils and Garder's gland. Research results showed that in tissues in places of the vaccine administration there were developed hemorrhages, serous inflammatory edema, necrobiosis and necrosis of muscular fibers, inflammatory cellular infiltration, and moderate plasmocytic reaction. At a later stage at the places of damaged structures there was noted the development of granulation tissue with presence of some lymphoid nodules. In response to vaccination in the central organs of poultry immunity there was stated the sinuated changes of morphological indexes. Immunization of chickens against infectious bursal disease, infectious bronchitis and Newcasle disease promoted the increasing of absolute weight of lien, activation of blastic and plasmocytic reaction in bursa of Fabritius and lien and did not render substantial influence on immunomorphological indexes of cecal tonsils and Garder's gland

Morphological indexes in immune system organs of piglets which were vaccinated which were immunized by SPS vaccine (against salmonellosis (Salmonella), pasteurellosis (Pasteurella) and streptococcus) in combination with immunostimulative drugs and without them were studied in the conditions of the Republic of Belarus. Piglets of the first group were vaccinated against salmonellosis, pasteurellosis and streptoccocusis by SPS vaccine. Piglets of the second group were vaccinated by SPS vaccine in combination with vitamin C. Piglets of the third group were vaccinated by SPS vaccine with immunotstimulant sodium thiosulphate. The fourth (control) group of animals was administrated with normal saline. Animals were immunized twice, intramuscularly with 7 day interval and in dose 4 ml (primary), 5 ml (secondary). Vitamin С was added in dose 0,05g per head. Research results showed that immunization by SPS vaccine with sodium thiosulphate and Vitamin С activated the morphological reaction of immune system. Application of Vitamin С and sodium thiosulphate (30% concentration) in combination with SPS vaccine promoted the increasing of lymphoid nudels in lymphodnudus and lien, increasing of plasma cells quantity in 1,3-2,2 times, activation of micro- and macrophage reaction in 1,5-2,8 times in comparison with vaccinated animals

Experimental application of a vaccine against salmonellosis, pasteurellosis, and streptococcosis (SPS) for piglets of 30-35 days was realized in the conditions of the Republic of Belarus. Research results showed that administrationof sodium thyosulphate and vitamin С in combination with SPS vaccine promoted the statistically-valid increasing of preventive properties of blood serum of piglets and increasing of titers of specific antibodies to agents of pasteurellosis and a salmonellosis in 1,3-2 times, as well as increasing in blood serum of quantity of immunoglobulins and to reduction of quantity of globulins.

After immunization of 30-35 day old piglets against a salmonellosis, pasteurellosis and streptococcosis (SPS) with special produced in the Republic of Belarus SPS vaccine in combination with immunostimulant sodium thyosulfate and vitamin C there was observed an activation myeloblastic hemopoiesis and lowering of erythropoeisis.

In the conditions of the Republic of Belarus there was produced a trial series of hyperimmune serum against livestock animals colibacillosis and there was realized the quality control of the analysed biological preparation. Research results proved that the developed serum was sterile, safe and had high immunogenetic activity. In course of quality control there were considered the following indexes: determination of physiognomy, colour, presence of visually accessible particulate matters; determination of safety and harnfullness; determination of activity; determination of concentration of hydrogen ions; determination of sterility; determination of serum volume in consumption package. For serum production there were used strains E.coli O9, O78, O0, O117, O26, O139, O15, O41, O55, O115, O141, O8, K88, K99, F41, 987P. For the production of trial series there was realized cultivation of Escherichia on the special nutritive media. There used bulls as serum producers

Research of immune activity of an experimental series of inactivated vaccine against swine pasteurellosis (Pasteurella) with application of adjuvants of Sepptic Montanida ISA 70 and Montanida ISA 206 was realized in the conditions of the Republic of Belarus. In the capacity of water phase there were used serovars A, B, and D Pasteurella multocida. Research results proved the possibility of application of adjuvants of Sepptic company for the development of vaccine against pasteurellosis. The vaccine with the presented adjuvants after its application did not cause after-trouble effects. In course of immunization there was noted the formation long-lived continuous immunity that was proved by high titres of specific anti-bodies in blood serum in all experimental groups of piglets. Single application of the proposed vaccine in doses 2 or 3 ml per animal proved to be effective. Also it was efficient to apply it twice in doses 1 and 2 ml per animal. For the vaccine production it was proposed to use adjuvant Montanida ISA 206 which did not have high reactogenicity

Studying of efficiency of application of modified scheme of hyperimmunization of bull-producers with a designer salmonella polyantigenin in comparative analysis with the production scheme of hyperimmunization was realized in the conditions of the Republic of Belarus. Research results showed that engineered polyvalent antigen for hyperimmunization of producer bulls contained in their formula the following serovariants of salmonella in 1:1 ratio: S. typhimurium 371, S. dublin 373, S. choleraesuis 370, and S. enteritidis CB. The obtained antigen was sterile, harmless and had pH 7,3. Hyperimmunization of producer bulls in the experimental scheme revealed in increasing of leukocytes quantity on 57,3 %, banded neutrophils on 86,7 %, B-lymphocytes - on 42,2 %, content of total protein - on 25,6 %, content of Ig G - on 77,8 %, lg M - on 51,6%. After the fifth administration of antigen there was stated the lowering of agglutinating activity of blood serum on 25%, content of Ig G - on 54,4%, Ig M - on 14,6 %, that indicated to the inefficiency of antigen administration and became the basis for termination of hyperimmunization cycle. Immunobiological reaction of bull-producers which were hyperimmunized by the suggested scheme differed from reaction of animals which were hyperimmunized by the production scheme: content of total protein increased on 10,1%, protein content - on 27,3 %, content of lg G - on 24,8 %, lg M - in 23,7%