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A comparison of single dose efficacy of Mycoplasma hyopneumoniae bacterin in swine farms with different serological patterns of PRRSV and PCV2
2008
Kim, H.K. (Seoul National University, Seoul, Republic of Korea) | Moon, H.J. (Seoul National University, Seoul, Republic of Korea) | Kim, E.M. (Seoul National University, Seoul, Republic of Korea) | Yang, J.S. (Seoul National University, Seoul, Republic of Korea) | Park, S.J. (Seoul National University, Seoul, Republic of Korea) | Luo, Yuzi (Seoul National University, Seoul, Republic of Korea) | Lee, C.S. (Green Cross Veterinary Products, Yongin, Republic of Korea) | Song, D.S. (Green Cross Veterinary Products, Yongin, Republic of Korea) | Kang, B.K. (Green Cross Veterinary Products, Yongin, Republic of Korea) | Lee, J.B. (Hankuk Academy of Foreign Studies, Yongin, Republic of Korea) | Park, B.K. (Seoul National University, Seoul, Republic of Korea), E-mail: parkx026@snu.ac.kr
This study was to evaluate the efficacy of single dose Mycoplasma hyopneumoniae (M. hyo)-vaccination in the swine farms which had different serological patterns of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). A minimum of 240 pigs from each farm was applied, allocating M. hyo vaccinated and control groups. The PRRSV and PCV2 infections were analyzed by serological method (commercial ELISA kit). After administrating pigs a single dose of M. hyo vaccine or control saline at 3 weeks of age, serum antibodies to M. hyo, PRRSV and PCV2 were monitored at 4, 10, 16 and 22 weeks of age. Mortality, weight changes feed conversion ratio (FCR) and lung score were also evaluated. A single-dose vaccination of M. hyo bacterin was efficacious to reduce mycoplasmal lung lesions and induce good humoral immune response. However, FCR was improved only in one of the three farms where showed seronegative status to both PRRSV and PCV2 in the period from 4 to 16 weeks of age. These results might imply that M. hyo vaccine alone could not overcome the PRRSV and PCV2 infection-associated wasting in the field condition. Therefore, the control of PRRSV and PCV2 should be considered to obtain the better effects of M. hyo vaccination.
Afficher plus [+] Moins [-]Effects of anti-tick cocktail vaccine against Rhipicephalus appendiculatus
2008
Imamura, S.(Hokkaido Univ., Sapporo (Japan)) | Konnai, S. | da Silva Vaz, I.Jr. | Yamada, S. | Nakajima, C. | Ito, Y. | Tajima, T. | Yasuda, J. | Simuunza, M. | Onuma, M. | Ohashi, K.
Rhipicephalus appendiculatus serpin-3 (RAS-3), R. appendiculatus serpin-4 (RAS-4) and a 36kDa immuno-dominant protein of R. appendiculatus (RIM36) were reported as candidate antigens for the anti-tick vaccine to control ixodid ticks. In the present study, we generated recombinant proteins of RAS-3 (rRAS-3), RAS-4 (rRAS-4) and RIM36 (rRIM36), and assessed their potency as an anti-tick cocktail vaccine in cattle model. RT-PCR analysis showed that RAS-3, RAS-4 and RIM136 transcripts were detected in both adult male and female ticks during feeding. Immunization of cattle with the combination of rRAS-3, rRAS-4 and rRIM36 had raised antibodies against all recombinants and anti-sera had reacted with the molecules from the tick salivary gland extract. Tick infestation challenge demonstrated protective immunity against female ticks, resulting in mortality rates of 39.5 and 12.8 % for the vaccinated and control groups, respectively. Moreover, the mortality rate of Theileria parva-infected female ticks was 48.5 and 10.8 % in the vaccinated and control group, respectively. In order to evaluate the levels of pathogen transmission capacity by T. parva-infected ticks fed on immunized cattle, the occurrence of T. parva in the bovine parotid lymph node and peripheral blood was also determined and quantified by real-time PCR. Although the infection with T. parva could not be protected by the vaccine, the occurrence of pathogen in peripheral blood was delayed 1 to 2 days after the infestation challenge in vaccinated group. These results suggest that this cocktail vaccine plays a role in the prevention of tick infestation.
Afficher plus [+] Moins [-]Development of vaccine strains of H5 and H7 influenza viruses
2008
Soda, K.(Hokkaido Univ., Sapporo (Japan)) | Sakoda, Y. | Isoda, N. | Kajihara, M. | Haraguchi, Y. | Shibuya, H. | Yoshida, H. | Sasaki, T. | Sakamoto, R. | Saijo, K. | Hagiwara, J. | Kida, H.
To establish vaccine strains of H5 and H7 influenza viruses, A/duck/Hokkaido/Vac-1/04 (H5N1) [Vac-1/04 (H5N1)], A/duck/Hokhaido/Vac-3/07 (H5N1) [Vac-3/07 (H5N1)], and A/duck/Hokkaido/Vac-2/04 (H7N7) [Vac-2/04 (H7N7)] were generated from non-pathogenic avian influenza viruses isolated from migratory ducks. Vac-1/04 (H5N1) and Vac-3/07 (H5N1) were generated by genetic reassortment between H5N2 or H5N3 virus as an HA gene provider and H7N1 or H6N1 virus as an NA gene provider. Vac-2/04 (H7N7) was a genetic reassortant obtained using H7N7 and H9N2 viruses to give high growth character of the H9N2 virus in chicken embryonated eggs. The results of sequence analyses and experimental infections revealed that these H5N1 and H7N7 reassortant viruses were non-pathogenic in chickens and embryos, and had good growth potential in embryonated eggs. These viruses should be useful to develop vaccines against H5 and H7 highly pathogenic avian influenza viruses.
Afficher plus [+] Moins [-]Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain
2008
Daud, N.H.A.(Hokkaido Univ., Sapporo (Japan)) | Kariwa, H. | Tkachenko, E. | Dzagurnova. T. | Medvedkina, O. | Tkachenko, P. | Ishizuka, M. | Seto, T. | Miyashita, D. | Sanada, T. | Nakauchi, M. | Yoshii, K. | Maeda, A. | Yoshimatsu, K. | Arikawa, J. | Takashima, I.
Puumala virus (PUUV), a causative agent of hemorrhagic fever with renal syndrome (HFRS), is prevalent in Europe and European Russia. No vaccine has been developed for PUUV-associated HFRS, primarily because of the low viral yield in cultured cells. A PUUV strain known as DTK/Ufa-97 was isolated in Russia and adapted for growth in Vero E6 cells maintained in serum-free medium. The DTK/Ufa-97 strain produced a higher viral titer in serum-free medium, suggesting that it may prove useful in the development of an HFRS vaccine. When PUUV-infected Vero E6 cells were grown in serum-free medium, the DTK/Ufa-97 strain yielded more copies of intracellular viral RNA and a higher viral titer in the culture fluid than did the Sotkamo strain. Phylogenetic analysis revealed that PUUVs can be classified into multiple lineages according to geographical origin, and that the DTK/Ufa-97 strain is a member of the Bashkiria-Saratov lineage. The deduced amino acid sequences of the small, medium, and large segments of the DTK/Ufa-97 strain were 99.2% to 100%, 99.3% to 99.8%, and 99.8% identical, respectively, to those of the Bashkirian PUUV strains and 96.9%, 92.6%, and 97.4% identical, respectively, to those of the Sotkamo strain, indicating that the PUUVs are genetically diverse. However, DTK/Ufa-97 and other strains of PUUV exhibited similar patterns of binding to a panel of monoclonal antibodies against Hantaan virus. In addition, diluted antisera (i.e., ranging from 1:160 to 1:640) specific to three strains of PUUV neutralized both homologous and heterologous viruses. These results suggest that the DTK/Ufa-97 strain is capable of extensive growth and is antigenically similar to genetically distant strains of PUUV.
Afficher plus [+] Moins [-]Режимы инактивации и соотношение компонентов при изготовлении инактивированной вакцины против пастереллеза свиней
2008
Verbitskij, A.A. | Gvozdev, S.N., Vitebsk State Academy of Veterinary Medicine (Belarus)
The data on an inactivation scheme for the agent of porcine pasteurellosis, development of the inactivated vaccine and ratio of the antigen and adjuvant are presented. As a result of the realized research it has been established, that for Pasteurella inactivation it is necessary to use formalin in concentration of 0,5% at an exposition at 1 hour. At vaccine producing it is necessary to apply an antigene parity to adjuvant 50:50 as the received vaccine does not concede on all parameters to the vaccine in which a parity of components accordingly 40:60. Thus received vaccine possesses smaller reactogenicity and more adjuvanticity. One of the basic conditions of reception of homogeneous stable emulsion is warming up of water and oil phases to temperature 30 deg С (+/-1 deg С). | Представлены сведения о разработке схемы инактивации возбудителя для изготовления инактивированной вакцины, а также о выборе соотношения антигена и адъюванта. В результате проведенных исследований было установлено, что для инактивации пастерелл следует использовать формалин в концентрации 0,5% при экспозиции в 1 час. При изготовлении вакцины необходимо применять соотношение антигена к адъюванту 50:50, так как полученная вакцина не уступает по всем параметрам вакцине, в которой соотношение компонентов соответственно 40:60. При этом полученная вакцина обладает меньшей реактогенностью и большей иммуногенностью. Одним из основных условий получения однородной стабильной эмульсии является подогревание водной и масляной фаз до температуры 30 deg С (+/-1 deg С).
Afficher plus [+] Moins [-]Влияние инактивированной вакцины против пастереллеза пушных зверей на организм норок
2008
Birman, B.Ya. | Poloz, S.V. | Andrusevich, A.S., The National Academy of Sciences, Minsk (Belarus). The S.N.Vyshelesskij Inst. of Experimental Veterinary Medicine
The data about influence of the developed home-produced inactivated vaccine against pasteurellosis of fur animals on blood, biochemical, immunity indicators at mink are cited. It is established, that application of the given preparation conducts to formation of the necessary immune answer. The developed domestic inactivated vaccine against pasteurellosis of fur animals is a harmless and areactogenic preparation. This vaccine does not render negative action on hematological and biochemical indicators of mink blood. They are identical to indicators in control group of fur animals. Immunization with this vaccine stimulates cellular and humoral immunity factors that testifies to formation of the proof immune answer | Приведены данные о влиянии разработанной отечественной инактивированной вакцины против пастереллеза пушных зверей на гематологические, биохимические, иммунологические показатели норок. Установлено, что применение данного препарата ведет к формированию необходимого иммунного ответа. Разработанная отечественная инактивированная вакцина против пастереллеза пушных зверей является безвредным и ареактогенным препаратом. Инактивированная вакцина против пастереллеза пушных зверей не оказывает отрицательного действия на гематологические и биохимические показатели крови норок. Они идентичны показателям в контрольной группе пушных зверей. Иммунизация инактивированной вакциной против пастереллеза пушных зверей стимулирует клеточные и гуморальные факторы иммунитета, что свидетельствует о формировании стойкого иммунного ответа
Afficher plus [+] Moins [-]Ветеринарно-санитарные показатели качества и безопасности мяса при использовании различных вакцин против рожи свиней
2008
Dremach, G.Eh. | Aleksin, M.M. | Rudenko, L.L., Vitebsk State Academy of Veterinary Medicine (Belarus)
There was carried out the veterinary and sanitary examination of meat of young pigs vaccinated against swine erysipelas by a vaccine of a pilot batch. The meat testing results from animals under investigation show that the used vaccines reveal no negative influence on organoleptic and certain physical-chemical characteristics of meat; and by relative biological value the meat of young pigs at the use of concentrated vaccine of Konev matrix and the depot vaccine against swine erysipelas somewhat surpasses the analogous parameter of meat taken from the intact animals. Meat from animals of all groups is free from food borne toxic infections agents and toxicosis which characterizes it as a quality and safe food product. In the tabular form some physical and chemical and biological indicators of meat from young pigs involved in experiences are presented. | Целью работы явилось проведение ветеринарно-санитарной экспертизы мяса молодняка свиней, вакцинированного против рожи свиней вакциной опытной серии. Полученные результаты исследований мяса от подопытных животных указывают на то, что применяемые вакцины не оказывают отрицательного влияния на его органолептические и некоторые физико-химические показатели, а по показателям относительной биологической ценности мясо молодняка свиней при использовании концентрированной вакцины из матрикса Конева и депонированной вакцины против рожи свиней несколько превосходит аналогичный показатель мяса от интактных животных. Мясо от животных всех групп не содержит возбудителей пищевых токсикоинфекций и токсикозов, что характеризует его как качественный и безопасный пищевой продукт. В табличной форме представлены некоторые физико-химические и биологические показатели мяса от молодняка свиней, задействованных в опытах.
Afficher plus [+] Moins [-]Динамика индикаторных ферментов сыворотки крови, поджелудочной железы и печени ремонтного молодняка кур, вакцинированного против инфекционного ларинготрахеита
2008
Sobolev, D.T. | Elisejkin, D.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
There was studied the activity of alaninaminotransferase and aspartataminotransferase, alkaline phosphatase, gamma-glutamiltranspeptidase, lactate dehydrogenase, cholinesterase, alpha amylase in blood serum, liver and pancreas of replacement young hens. At carrying out of immunization of replacement young hens by a vaccine against infectious laryngotracheitis, developed in Institute of experimental veterinary science, reaction from the party not only bodies of immune system, but also such bodies, as liver and pancreas, takes place. Biochemical reaction of the specified bodies to immunization is a consequence of adaptability of an organism to vaccinal stress. Biochemical changes of display enzymes in blood serum, pancreas and liver are found out. It was marked increase of activity of the given enzymes in liver, pancreas and blood serum so, activity of alaninaminotransferase and aspartataminotransferase, alkaline phosphatase, gamma-glutamiltranspeptidase, lactate dehydrogenase increased at birds of trial group in 1,3-1,9 times in comparison with the control. In the tabular form data on activity of alaninaminotransferase, aspartataminotransferase, alkaline phosphatase in liver, pancreas and blood serum of repair young growth of the hens vaccinated against infectious laryngotracheitis are presented. | Целью наших исследований явилось изучение активности аланин- и аспартатаминотрансфераз, щелочной фосфатазы, гамма-глутамилтрансферазы. лактатдегидрогеназы, холинэстеразы, альфа-амилазы, в сыворотке крови, печени и поджелудочной железе ремонтного молодняка кур. При проведении иммунизации ремонтного молодняка кур вакциной против инфекционного ларинготрахеита, разработанной в Институте экспериментальной ветеринарии им. С.Н. Вышелесского НАН РБ имеет место реакция со стороны не только органов иммунной системы, но и таких органов, как печень и поджелудочная железа. Биохимическая реакция указанных органов на иммунизацию является следствием адаптивности организма к вакцинному стрессу. Выяснены биохимические изменения индикаторных ферментов в сыворотке крови, поджелудочной железе и печени. Отмечалось сочетанное повышение активности данных ферментов в печени, поджелудочной железе и сыворотке крови, так, активность АлТ, АсТ, ЩФ, ЛДГ, ГГТФ повышалась у птиц опытной группы в 1,3-1,9 раза по сравнению с контролем. В табличной форме представлены данные по активности аланинаминотрансферазы (АлТ), аспартатаминотрансферазы (АсТ), щелочной фосфатазы (ЩФ) в печени, поджелудочной железе и сыворотке крови ремонтного молодняка кур, вакцинированных против ИЛТ
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