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PREVALENCE OF Β- LACTAMASE PRODUCING STAPHYLOCOCCUS AUREUS IN SLAUGHTERED Texte intégral
2008
Adnan M. AL- Rodhan
Fifty liver and lung samples (25 for each) were examend for the presence of Staphylococcus aureus . The rate of S. aureus isolates were 60 and 56% in livers and lungs respectively. Identification of S. aureus isolates were performed by studying, it's cultural characteristics on mannitol salt agar and testing their pathogenicity factors (Coagulase, haemolysis and B-lactmase production), 72.4% of isolates were coagulase positive, 58.6% were β-lactamase producer, 62.1% were β-haemolytic and 37.9% were α-haemolytic. There was a difference in the ablity of S. aureus isolates to produce B-lactamase concerning the time of decolourization when iodometric assay was used.
Afficher plus [+] Moins [-]EFFECT OF DICHLORVOS PESTICIDE ON FERTILITY OF LABORATORY MALE MICE (Mus musculus L.) Texte intégral
2008
Faris Shaker Kata
Dichlorvos is one of organophosphate insect pesticides which is widely distributed in environment. This study deals with the effect of this pesticide on sperms number , sperm abnormalities and spermatogenesis in laboratory male mice (Mus musculus L.) which were treated with (0.1 mg/day , 0.05 mg/day ) of the pesticide for a period of 15 day. intraperitoneal injection of Dichlorvos in laboratory mice resulted in a significant decrease in sperms number with both doses as compared with the control group. The result also showed a significant increase of sperm abnormalities with both doses. Moreover, pesticide injection caused a significant decrease in spermatogonia and primary spermatocyte with both doses whereas the statistical analysis did not show any significant difference in tubular diameter when compared with the control group.
Afficher plus [+] Moins [-]EFFECT OF ASPIRIN AS ANTIFUNGAL DRUG AGAINST SOME OPPORTUNISTIC FUNGI Texte intégral
2008
Alia | A. Al- Bader
The effect of aspirin( non steroidal anti-inflammtory drug a cyclooxygenase inhibitor) as antifungal has been studied against some opportunistic fungi : Aspergillus flavus , A. niger , A. terreus ,Cryptococcus neoformans, Penicillium sp . and Trichoderma sp. Aspirin was showed a potent activity against all tested fungi in vitro . Aspirin gives the greatest effects in a concentration of 1000 µg , 2000 µg and 3000 µg causing 100% inhibition .
Afficher plus [+] Moins [-]A SURVEY ON CAMEL HYDATIDOSIS IN NAJAF ABATTOIR Texte intégral
2008
Raad A. Ismail
The offal’s of (380) slaughtered camels were examined for Hydatidsis in Najaf abattoir between January and December (2002). The total incidence of infection was (17.9%) and it was much higher in older camels (19.23%) while it was (9.16%) in younger ones. The distribution of cysts in lungs, livers, and spleens was (16.5%), (1.57%), and (0.53%) respectively. The fertility rate was (50.2%) which reflect their significance in the epidemiology of the parasite and reveal that those dromedaries Hydatidosis could play a significant role in the epidemiology of infection
Afficher plus [+] Moins [-]Pentastomid parasites in fish in the Olifants and Incomati River systems, South Africa Texte intégral
2008
Wilmien J. Luus-Powell | Antoinette Jooste | Kerstin Junker
Pentastomid parasites in fish in the Olifants and Incomati River systems, South Africa Texte intégral
2008
Wilmien J. Luus-Powell | Antoinette Jooste | Kerstin Junker
During parasitological field surveys of freshwater fish, sebekiid and subtriquetrid pentastome larvae were recovered from the body cavity or swim bladder of several fish species from various localities in Limpopo and Mpumalanga Provinces, South Africa. Sebekia wedli was recovered from the body cavity of Marcusenius macrolepidotus (Mormyridae) from Flag Boshielo Dam, Limpopo Province, and Alofia sp. and Subtriquetra rileyi were found in the swim bladder of Oreochromis mossambicus (Cichlidae) from the Phalaborwa Barrage, Limpopo Province. The latter species was also collected from the swim bladder of O. mossambicus in dams in the Phalaborwa region and the Ga-Selati River, Limpopo Province. A single specimen of Sebekia okavangoensis was present in the body cavity of Clarias gariepinus (Clariidae) in a dam on a sugarcane farm in the Komatipoort region, Mpumalanga Province. Pentastomid infections in the Mormyridae and Clariidae represent new host records.
Afficher plus [+] Moins [-]Pentastomid parasites in fish in the Olifants and Incomati River systems, South Africa Texte intégral
2008
Luus-Powell, Wilmien J.(University of Limpopo Department of Biodiversity) | Jooste, Antoinette(University of Limpopo Department of Biodiversity) | Junker, Kerstin(University of Pretoria Department of Veterinary Tropical Diseases)
During parasitological field surveys of freshwater fish, sebekiid and subtriquetrid pentastome larvae were recovered from the body cavity or swim bladder of several fish species from various localities in Limpopo and Mpumalanga Provinces, South Africa. Sebekia wedliwas recovered from the body cavity of Marcusenius macrolepidotus (Mormyridae) from Flag Boshielo Dam, Limpopo Province, and Alofia sp. and Subtriquetra rileyi were found in the swim bladder of Oreochromis mossambicus (Cichlidae) from the Phalaborwa Barrage, Limpopo Province. The latter species was also collected from the swim bladder of O. mossambicus in dams in the Phalaborwa region and the Ga-Selati River, Limpopo Province. A single specimen of Sebekia okavangoensis was present in the body cavity of Clarias gariepinus (Clariidae) in a dam on a sugarcane farm in the Komatipoort region, Mpumalanga Province. Pentastomid infections in the Mormyridae and Clariidae represent new host records.
Afficher plus [+] Moins [-]Bovine intestinal cellular responses following primary and challenge infections with Calicophoron microbothrium metacercariae Texte intégral
2008
Mavenyengwa, M.(University of Zimbabwe Department of Paraclinical Veterinary Studies Faculty of Veterinary Science) | Mukaratirwa, S.(University of KwaZulu-Natal School of Biological and Conservation Sciences) | Obwolo, M.(University of Zimbabwe Department of Paraclinical Veterinary Studies Faculty of Veterinary Science) | Monrad, J.(Danish Centre for Experimental Parasitology)
This study was carried out to establish whether cattle can develop resistance to re-infection by Calicophoron microbothrium by assessing the response of intestinal mucosal globule leukocytes, eosinophils, mast cells and basophils, and the establishment of the parasite in the host. A total of 24 1-year-old Tuli steers were randomly divided into four groups of six animals each and infected with C. microbothrium metacercariae. On the first day of the study, animals in Groups I and II were immunized with 5 000 metacercariae and then challenged with 15 000 metacercariae on Day 150 post-immunization. Animals in Group III were immunized with 15 000 metacercariae at the same time that Groups I and II animals were challenged to act as a positive control group. Animals in Group IV were left uninfected and acted as a negative control group. Three animals from each group were slaughtered on Day 28 post-challenge and the remainder were slaughtered on Day 42 post-challenge. The established amphistomes were recovered and histopathological and cytological examinations were done on the jejunum, duodenum, abomasum and the rumen. The establishment rates of the challenge infection in the immunized and challenged groups were lower and ranged from 0 to 0.2 % as compared to 6 %> from naive animals infected as positive controls. Animals immunized and then challenged with C. microbothrium had significantly higher eosinophil, mast cell and globule leukocytes counts in the intestinal mucosa (P < 0.05) as compared to those of the control group. The study indicates that cattle can develop resistance to C. microbothrium re-infection and that eosinophils and mast cells may be important cells in the rejection of the parasite.
Afficher plus [+] Moins [-]Studies on effects of lactose on experimental Trypanosoma vivax infection in Zebu cattle. 1. Plasma kinetics of intravenously administered lactose at onset of infection and pathology Texte intégral
2008
Fatihu, M.Y.(Ahmadu Bello University Department of Veterinary Pathology and Microbiology) | Adamu, S.(Ahmadu Bello University Department of Veterinary Pathology and Microbiology) | Umar, I.A.(Ahmadu Bello University Department of Biochemistry) | Ibrahim, N.D.G.(Ahmadu Bello University Department of Veterinary Pathology and Microbiology) | Eduvie, L.O.(Ahmadu Bello University National Animal Production Research Institute) | Esievo, K.A.N.(Ahmadu Bello University Department of Veterinary Pathology and Microbiology)
Lactose in normal saline was administered intravenously to a group of Zebu cattle infected with Trypanosoma vivax to determine the blood plasma kinetics at onset of an experimental infection and its ability to protect tissues against damage as part of preliminary studies to determine its suitability for use in the treatment of trypanosomosis. Significantly (P < 0.01) higher lactose concentrations were observed in the T. vivax-infected bulls at 30 min and 1 h (P < 0.05) post-infection (p.i.) and by 4 h p.i. the plasma lactose remained above the level prior to infusion, after which it fell slightly below the pre-infusion level in the uninfected group. Calculated pharmacokinetic parameters revealed delayed excretion of lactose in the T. w'vax-infected group soon after infection. The total body clearance (CI B) was significantly (P < 0.05) reduced. The biological half-life (t ½), elimination rate constant (k e) and apparent volume of distribution (Vd) were relatively decreased (P > 0.05) as a result of the T. vivax infection. Retention of lactose in the plasma was attributed to decreased plasma clearance. It is suggested that the presence of trypanosomes in circulation rather than organic lesions could have been responsible for the delay observed in the excretion of lactose. At 12 weeks p.i., when the experiment was terminated, the group infected and given lactose infusion (despite higher parasitaemia) had no gross or histopathological lesions in the brain, spleen, lymph nodes, heart, kidneys, liver and testes. However, the group infected but not infused with lactose were emaciated, had pale mucosae, watery blood, general muscular atrophy, serous atrophy of coronary fat and other adipose tissue, hepatomegaly, splenomegaly, swollen and oedematous lymph nodes, all of which are suggestive of trypanosomosis. Histopathological lesions included narrowing of Bowman's space and hypercellularity of glomerular tufts in the kidneys with the mean glomerular tuft nuclear indices (GTNs) in the group significantly higher (P< 0.01) than the mean GTNs of the lactose-infused and control bulls. Degenerative changes occurred in the myocardium, spleen, testes and epididymides. The tesicular and epididymal lesions are indicative of male reproductive dysfunction.
Afficher plus [+] Moins [-]A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses Texte intégral
2008
Abolnik, C.(ARC-Onderstepoort Veterinary Institute)
A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.
Afficher plus [+] Moins [-]Epidemiological survey on gastro-intestinal and blood-borne helminths of dogs in north-east Gabon Texte intégral
2008
Davoust, B.(Direction régionale du service de santé des armées de Toulon) | Normand, T.(Ecole nationale vétérinaire de Lyon Laboratoire de parasitologie ,Centre national d'instruction cynophile de la Gendarmerie Service vétérinaire) | Bourry, O.(Centre international de recherches médicales) | Dang, H.(Ecole nationale vétérinaire de Lyon Laboratoire de parasitologie) | Leroy, E.(Centre international de recherches médicales ,Institut de recherche pour le développement) | Bourdoiseau, G.(Ecole nationale vétérinaire de Lyon Laboratoire de parasitologie)
A survey of helminth parasites was carried out on 198 dogs living in almost complete liberty in villages in the northeast of Gabon. Faeces and blood samples were collected and analysed. Dirofilaria immitis antigen was detected in 13.6 % of dogs using the SNAP 3Dx® test, a commercially available enzyme-linked immunosorbent assay (ELISA). Faecal examination revealed that 91.4 % of dogs were infected by intestinal helminths. Ascarids were found in 58.5 % of the samples. Trichuris vulpis was observed in 49.5 %> of cases, and Uncinaria spp. and Ancylostoma spp. in 34.8 %>, Spirocerca lupi in 25.3 % and Capillaria spp. in 10.6 %. Cestode embryophores were found in 8.6 % of the samples.
Afficher plus [+] Moins [-]Evaluation of larvicides in developing management guidelines for long-term control of pest blackflies (Diptera: Simuliidae) along the Orange River, South Africa Texte intégral
2008
R.W. Palmer | N.A. Rivers-Moore
Evaluation of larvicides in developing management guidelines for long-term control of pest blackflies (Diptera: Simuliidae) along the Orange River, South Africa Texte intégral
2008
R.W. Palmer | N.A. Rivers-Moore
In 2000 and 2001 Orange River levels were higher than normal: associated serious outbreaks of blackfly had a substantial detrimental impact on the local economy. The poor control was attributed to the suspected development of larval resistance to temephos. A long-term solution to blackfly control, through the identification of a suitable replacement to temephos for use during high flow conditions, was proposed. This study, however, failed to identify or register a suitable larvicide for use during high flow conditions. Although permethrin was highly effective against blackfly larvae, it was rejected because of its detrimental impacts on non-target fauna. Various formulations of locally produced dry Bacillus thuringiensis var. israelensis (B.t.i.) were tested, but these were ineffective against blackflies. The study also confirmed that resistance to temephos has developed among Simulium chutteri in the middle and lower Orange River. The feasibility of "reversing" the resistance to temephos through the use of the synergist piperonyl butoxide (PBO) was investigated, but the results were not favourable. Furthermore, PBO was highly toxic to blackflies and non-target organisms, and was not recommended for further testing. This means that B.t.i. currently remains the only symptomatic measure of treatment currently applied. Although resistance to B.t.i. has not been reported for blackflies elsewhere in South Africa, there is a need to remain vigilant and to implement an operational strategy that minimizes the risks of resistance developing.
Afficher plus [+] Moins [-]Evaluation of larvicides in developing management guidelines for long-term control of pest blackflies (Diptera: Simuliidae) along the Orange River, South Africa Texte intégral
2008
Palmer, R.W.(Nepid Consultants) | Rivers-Moore, N.A.(Rhodes University Institute for Water Research)
In 2000 and 2001 Orange River levels were higher than normal: associated serious outbreaks of blackfly had a substantial detrimental impact on the local economy. The poor control was attributed to the suspected development of larval resistance to temephos. A long-term solution to blackfly control, through the identification of a suitable replacement to temephos for use during high flow conditions, was proposed. This study, however, failed to identify or register a suitable larvicide for use during high flow conditions. Although permethrin was highly effective against blackfly larvae, it was rejected because of its detrimental impacts on non-target fauna. Various formulations of locally produced dry Bacillus thuringiensis var. israelensis (B.t.i.) were tested, but these were ineffective against blackflies. The study also confirmed that resistance to temephos has developed among Simulium chutteri in the middle and lower Orange River. The feasibility of "reversing" the resistance to temephos through the use of the synergist piperonyl butoxide (PEO) was investigated, but the results were not favourable. Furthermore, PBO was highly toxic to blackflies and non-target organisms, and was not recommended for further testing. This means that B.t.i. currently remains the only symptomatic measure of treatment currently applied. Although resistance to B.t.i. has not been reported for blackflies elsewhere in South Africa, there is a need to remain vigilant and to implement an operational strategy that minimizes the risks of resistance developing.
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