Streptococcus spp. comprising Streptococcus (S.) uberis and S. dysgalactiae strains is major cause of bovine mastitis from particularly well-managed or low somatic cell count herds that have successfully controlled contagious pathogens. In this study, antimicrobial susceptibility and genetic characteristics of S. uberis isolated from clinical or subclinical mastitis milk at 2003 were investigated. Eighty seven isolates of Streptococcus spp. were identified by the conventional biochemical methods. The antimicrobial susceptibility by disk diffusion method was determined for 46 S. uberis, 11 S. bovis, 10 S. oralis, 6 S. uberis and 14 other Streptococcus spp., Overall, the tested strains were susceptible to tetracycline (11.5%), amikacin (14.9%), streptomycin (16.1%), neomycin (26.4%), kanamycin (35.6%), gentamicin (65.2%), oxacillin (70.1%), ampicillin (75.9%), chloramphenicol (78.2%), and cephalothin (97.7%).

Staphylococci are Gram-positive, facultatively anaerobic cocci, normally found on the skin and mucosal surfaces of most warm-blooded animals and often involved in a wide variety of diseases in animals. Staphylococcal infections are treated with antibiotics and, consequently, antibiotic resistance and/or acquired resistance have developed. Staphylococcus (S.) intermedius and Staphylococcus (S.) aureus are 2 common veterinary isolates that are frequently associated with suppurative infections. This study was undertaken to examine antimicrobial susceptibility of S. aureus (23 isolates) and S. intermedius (160 isolates) isolated from dogs in Gwangju, Korea and investigate whether the antibiotic resistance of S. aureus and S. intermedius is effected by the site of isolation, age, and sex of dogs.

A case of multicentric high grade T cell lymphoma is reported in a 5-year-old male Maltese dog with generalized lymphadenopathy. The dog showed depression, anorexia, blindness, jaundice, arrhythmia, and hematuria for 8 months. Complete blood count and chemistry profile revealed anemia and increased alanine transferase, alkaline phosphatase, total bilirubin, and total cholesterol. Grossly, most of lymph nodes, spleen, and liver were enlarged and neoplastic masses were occupied in these tissues. Histologically, massive accumulation of small noncleaved neoplastic lymphocytes with high mitotic figures was observed in all lymph nodes and spleen.

Bone graft had been investigated previously to restore bone defects in orthopedics. The purpose of this study was to investigate the efficacy on new bone formation in bone autografts by treatment of implants. Cortical bone autografts were transplanted to midshaft of diaphyseal fibulae of 9 rabbits which were divided in 3 groups according to the treatment method of implants. Cortical bone implants for graft were treated with 3 different methods; freezing, freeze-drying, defat-freezing. Autografts were achieved by cross-transplantation method to bilateral fibulae of the presented rabbits after implant treatment procedures.

Measurement uncertainty could play an important role in the assessment of test results in laboratories and industries. We investigated measurement uncertainties possibly included in determination of flubendazole, a benzimidazole anthelmintic, in pork by HPLC. The concentration of flubendazole was 62.69 ng/g in a sample of pork. Uncertainty was estimated in the analytical procedure of flubendazole. A model equation was made for determination of flubendazole in pork. The four uncertainty components such as weight of sample, volume of sample, calibration curve, and recovery were selected to estimate measurement uncertainties.

Lactic acid bacteria (LAB) has been regarded as a useful microorganism and tried to manipulate plasmid DNA for increasing the usefulness. Although several methods have been developed is isolate plasmid DNA from Escherichia coli (E. coli), these methods were not sufficient to apply to LAB with exception of O'Sullivan's lysis method. So, we evaluated plasmid DNA extraction from LAB using general E. coli preparation methods and tried to improve the extraction yield and DNA purity by modifying O'Sullivan's alkaline lysis method.

This study reveals the unusual case reported for the first time on the selective non digestion of yellow mealworm (Tenebrio molitor) larvae by arowana fish. In January 2005, an Asian arowana (Scleropages formosus) (red variety), from Daesang Tropical Fish Corporation, Seoul, Korea, mortality was observed due to unknown cause. No putative causal factors were suggested by bacteriological and parasitological examinations. Internal examination of the dissected stomach showed some undigested debris with mandible parts of mealworms attached to the mucosal lining of stomach wall.

A 3-year-old female Cocker Spaniel dog acutely developed hepatopathy following ingestion of root of Cycad (Cycas revoluta). Seven hours after the ingestion, she showed acute continuous nausea, vomiting, and marked depression and was presented to the local veterinary clinic. At physical evaluation, the dog showed moderate weakness, pale mucous membrane, jaundice, and dehydration. Serum biochemical analysis revealed elevated alanine aminotransferase and aspartate aminotransferase, and mild azotemia. The dog was treated with intravenous fluid, antibiotics, and hepatic protectants for 10 days and recovered without any sequela.

A 14-month-old, neutered female Miniature Pinscher was referred due to severe renal failure. The dog was treated with hysterotomy and followed by ovariohysterectomy (OHE) 6 days before the presentation. Excretory urographic and ultrasonographic studies revealed bilateral hydroureter and hydronephrosis. Postmortem necropsy revealed that ligatures were placed around the caudal portion of bilateral ureters in front of entrance into the bladder. This case shows that identifying and protecting the ureters are important during hysterotomy with OHE as well as OHE.

Vulvar agenesis in an eight-month-old toy poodle dog is described. Urinary incontinence and dysuria were the main clinical signs. The morphology of the urogenital system was assessed with contrast radiography. The constructers of lower urinary tract and reproductive system except for the vulva were normal features. Through episiostomy, a perineal stoma, resembling a vulva, was created, resulting in complete resolution of the clinical signs.