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PATHOLOGICAL EFFECT OF DEXAMETHASONE ON HEALING OF RABBIT WOUND Texte intégral
2010
Aula Essmial H | Fayhaa Salim | Zainab Waheed
In this study 15 rabbits were divided into three groups ,each group contain five rabbit were submitted to surgical section in the skin layer 3-5 cm long after anasethesia and cleaning the part from the hair by sheaving . The surgical section was made by scable and then the wound sutured by silk band 2.5 cm and then administrated the dexamethasone 1 mg / Kg body weight of animals of second and third grougs of this study . After that the skin spicemen were taken for histopathological sections to preperd slid. The first group was considered as control with no administrated of dexamthasone , the second group treated as 1 mg / Kg body weight dexamthasone and sections were taken after 7th days , the third group were also treated with dexamthasone at the 1 mg /Kg and the skin sample were taken after 14 th days. The present study it was found that dexamthasone have no effect on the wound healing after 7th days or 14th days but the collagenization , epithelization and fibroblast content were less in the dexamthasone group compered with control group . The vasicularity and the degree of inflammatory cells were more intense in the dexamthasone group compared with control group.
Afficher plus [+] Moins [-]ISOLATION AND IDENTIFICATION OF ENTEROHEMOLYTIC ESCHERICHIA COLI FROM SLAUGHTERED COW'S LIVER AND LUNG Texte intégral
2010
Adnan M. Al-Rhodan
Liver and lung samples (25 for each) were collected from slaughtered cows and examend bacteriologicaly for the presence of Escherichia coli Eosin-Methylene Blue ( EMB ) and MacConkey agar positive culture (15 from liver and 12 from lung) were produced by cultivation of liver and lung samples Escherichia coli presence was confirmed biochemically in 53.3% of liver isolates and in 33.3% of lung isolates. Enterohemolytic activity was detected in 75%of both liver and lung isolates
Afficher plus [+] Moins [-]HYDROGEN PEROXIDE OF HONEY ANTIBACTERIAL EFFECT AGAINST COAGULATES-NEGATIVE STAPHYLOCOCCI Texte intégral
2010
Rana | A. Faaz
ABSTRACT The aim of this study was to determine the minimum active dilution of two standardized type, representative honeys from two bees farm for 7 clinical isolates of coagulates-negative staphylococci. An agar incorporation technique was used to determine the minimum active dilution, with dilution steps for 1% (v/v) honey and 5% (v/v) for sugar syrup .The plates were inoculated with 10 µL spots of cultures of the isolates. the pasture honey inhibited at mean SD3.2± 0.7% (v/v),3.3 ± 0.5% (v/v) for the Manuk honey and 30.9 ± 1.9% (v/v) for the sugar syrup. Typical honeys are about eight times more potent against coagulase-negative staphylococci than bacterial inhibition were due to their osmolarity alone, but also the activity of honey by having its content
Afficher plus [+] Moins [-]EFFECT OF LOW LEVEL LASER AND CHITOSAN POWDER ON THE ACCELIRATION OF WOUND HEALING IN RABBITS Texte intégral
2010
N. M. AL-Hayani
Open wounds have lost the barrier that protects tissues from bacterial invasion and allowfor the escape of vital fluids. The purpose of this study is to evaluate the enhancing oftherapeutic effect of Low Level Laser (LLL) and chitosan on the acceleration of openwound healing. Forty two adult rabbits were used. They divided into three equal groups (I,II and III). Wound was produced at the dorsal region by remove all thickness of skin atwidth of 3.5cm and of length 4 cm. Group I was left without treatment, while chitosanpowder and laser therapy were used in group II and III respectively. Results of two groupswere compared with control group. Histpathologicl results at the period of 3, 7, 14, 21 and28 days post operation reflected the presence of large number of fibroblast with formationof new blood vessels, also the collagen fiber become dense and regular in 14 days postoperation in irradiated groups. In 21days there was formation of new and normal epidermalclosed the rupture area of III group.
Afficher plus [+] Moins [-]STUDIES ON ANTIBACTERIAL ACTIVITY OF THE PLANT Texte intégral
2010
Asia Selman Abdullah | Amal Abdul-Jaleel Mahdi | Kawther Hawaaz
Alcohol extracts of the plant Salicornia herbacea L. were prepared, and found to be very effective against both Gram positive and Gram negative bacteria. Cytotoxicit againsty human red blood cells, were studied and found that alcohol extract is not toxic, has a toxicity of 2500mg/ml. Biological characteristics were studied by the measurement of minimum inhibitory concentrations on isolates of standard and pathological, Gram positive and Gram negative bacteria. Minimum lethal dose that kill 50% of population (LD50) of Alcohol extracts were measured and found to be 6000mg/kg. Stability of Alcohol extracts at different pH and temperature ranges were measured also.
Afficher plus [+] Moins [-]THE ANTIBACTERIAL ACTIVITY OF COLD AQUEOUS AND PIGMENT OF HIBISCUS ROSA SIENSIS EXTRACTS AGAINST GRAM POSITIVE AND NEGATIVE BACTERIA Texte intégral
2010
Fatima Saiwan | Zeenah Weheed Atwan
The antibacterial activity of aquaeous and purified pigment extracts of Hibiscus rosa siensis in concentration (20,50,100,200,250 mg) were tested against reference strains of Staphylococcus aureus and Esherichia coli . The preliminary qualitative tests showed that the two extracts have flavonoids, carbohydrates and glycosides ,while alkaloids found only in the aquaeous extract. Thin Layer Chromatography (TLC) showed the presence of anthocyanin pigment . Both extracts gave a clear activity against the tested strains with a minimal Inhibitory concentration reached to 20 mg/ml.
Afficher plus [+] Moins [-]PREVALENCE OF Listeria monocytogenes IN FROZEN FISH IN BASRAH CITY MARKETS Texte intégral
2010
Ghazi M. Jabir Al- Maliki
ABSTRACT The present study was performed on 80 samples of frozen fish . The samples were collected from different shops in Basrah city . Listeria spp. Was isolated (13%) of frozen fish , also they were counted in the examined samples. The mean counts were 8.75 x 102 CFU/g. of the fish . L. monocytogenes differentially identified from other Listeria species and could he isolated at (5%) in frozen fish . The drug susceptibility characterization of L. monocytogenes cleared that all isolates (100%) were resistant to Ampicilline and Gentanicine . while the other used antibiotics showed different degrees of antimicrobial sensitivity reactions for streptomycin , Tetracycline , Rifampicine and cefotexim
Afficher plus [+] Moins [-]ANATOMICAL AND HISTOLOGICAL STUDY OF THE SPLEEN IN IRAQI SHEEP(Awasi sheep) Texte intégral
2010
Eman M. Khalel
This study were carried out on 20 healthy of spleen healthy adult Awasi sheep.The anatomical study include study of length, width Thickness, weight of 10 spleen.The average measurements were 9.93+0.342;6.48+0.233;2.48+0.142 cm and 69+6.663 gram respectively while the histological study include the thickness of capsule and trabeculae ,splenic white pulp diameter ,lymphoid nodule diameter were determind on 10 spleens.The average measurements were 140.5±13.712; 82.75±9.7; 505±34.245; 315.25±23.185 μ respectively. The periarterial lymphatic sheath (PALS) containing One central artery that branched into 2-3 arteries.
Afficher plus [+] Moins [-]HISTOLOGICAL STUDY OF THE THYROID AND PARATHYROID GLANDS IN IRAQI BUFFALO "BUBALUS BUBALIS" WITH REFERRING TO THE SEASONAL CHANGES Texte intégral
2010
Amer M. Hussin | Muntadhar M. Al-Taay
The present study included the collection of thyroid and parathyroid glands from the abattoirs of middle of Iraq in Summer and Winter seasons 2006-2007. The samples were fixed by 10% formalin and processed by the routine histological techniques, then stained by H and E, PAS and Van Gieson stain, then studied under light microscope. Dimensions were measured by ocular micrometer. Analysis of data was done by T-test. The most striking result declared that the thyroid gland showed no differences in histological characters between both seasons as the buffalo was poor thermoregulator animal. It is tempting to speculate that the thyroid gland provide an adaptive advantage for survival of the buffalo in the nature arid environment. The parafollicular cells (C-cells) were sparse and found singly in the thyroid glands. Flat dark nuclei of myoepithelial cells were recognized around the thyroid follicles. This study was referred to the presence of accessory thyroid gland which contains simple follicular epithelium, and little amount of the colloid. Our current study revealed the presence of internal and external parathyroids. The oxyphilic cells in the internal parathyroid gland were not observed. Note: this work in part of Master Science Thesis, submitted to the College of Veterinary Medicine, Baghdad University.
Afficher plus [+] Moins [-]BLOOD PROGESTERONE AND ESTROGEN HORMONES LEVEL DURING PREGNANCY AND AFTER BIRTH IN IRAQI SHEEP AND GOAT Texte intégral
2010
Amin FAM and | Ibrahim NS | Alwan
Serum progesterone and estrogen concentrations were investigated during pregnancy and few days after birth. Blood samples were collected twice / month from 24 numbered animals ( 12ewes and 12 does) . serum was isolated and kept under -20C untill hormonal analysis. Enzyme - Linked Immunosorbent assay (ELISA) using (ELISA Reader Dona 3200). Progesterone concentrations of pregnant ewes and does were showed steadily increased to reach 24.9+ 2.5 ng/ml and 30.34+ 2.3ng/ml in ewes and does, during 4th month and declined to 0.6 and 2.5 ng/ ml after birth in ewes and does, respectively. Estrogen hormone levels in ewes were increased significantly during pregnancy to maximum 98.7+4.3ng/ml by 5th month and sharply declined to 4.1+0.06 after birth. While in does estrogen level increased significantly and steadily to 1150.6+ 6.23 pg/ml during last month of pregnancy and to 5.9+ 0.4 pg/ml after birth. The present work indicated levels of progesterone hormone increased during 1st and 2nd months in ewes and does, while the significant increased from 3rd month on . Estrogen reach maximum concentrations during last month in doe Which higher about 11 times than that of ewes .It is useful means to diagnosis pregnancy of ewes and does by hormonal methods after mating 20-30 days.
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