This study was undertaken to investigate the ameliorative effect of sodium selenite and nano-selenium on chicks exposed to heat stress. Ninety one day-old broiler chicks were divided into six groups each of 15 birds. Birds in group one and four were fed on basal diet and served as a control group, while birds in group two and five were fed on basal diet treated with 0.15 mg/Kg diet prepared selenium nanoparticles and birds in group three and six were fed on basal diet treated with 0.15 mg/Kg sodium selenite. On day 37 of experiment birds in 4th, 5th and 6th group were exposed to artificial heat stress at 40°C for 12 hours. Based on the results of total body weight gain and FCR lower parameters of body weight, body weight gain, feed conversion rate and higher feed intake ratio were observed in heat stressed chicks. Nano-selenium and Na selenite supplementation decreased the serum AST, ALT, urea, creatinine and MDA while the serum total protein, SOD, LZM, IgG, IgM and C3 were increased, also nano-selenium has ameliorative effect on hematological and histopathological findings. In conclusion, both nano-selenium and sodium selenite have an essential role in treatment of adverse effect of heat stress on chicks, with superiority of nano-selenium in improving anti- oxidative status and boosting immune response.

Meat industry is beginning to view meat shelf life as a serious issue. Organic acids and essential oils with antibacterial activities improve preservation of meat safety. Thus, the current study aimed to assess the preservation advantages of thyme oil, acetic acid, and a mixture of thyme oil and acetic acid (2% of each), as well as their effects on sensory characteristics, pH, total volatile basic nitrogen (TVBN), and thiobarbituric acid reactive substances (TBARS) of raw beef meat. The current study's findings demonstrated that treating raw beef meat with thyme oil, acetic acid at a concentration of 2% separately successfully lowered levels of APC, pH, TVBN, and TBARS and prolonged shelf life to 15 days when stored at 4°C. While the treatment with a combination of thyme oil and acetic acid at a concentration of 2% outperformed other treated and control groups leading to improving shelf life, and quality of raw beef meat. In conclusion, natural and organic preservatives may be utilized as an alternative to maintain meat and their products quality and extend their shelf life.

Unthriftiness is the major problems affect health and body performance of calves and induce many losses even with treatment. So, the aim of the present study was to investigate some adverse effects of unthriftiness on hematobiochemical parameters and some antioxidant enzymes as well as using Ambrosia maritime or synbiotics each alone with mineral mixture in treatment unthrifty calves. A total of 20 buffalo calves of 4-6 month old (5 healthy calves and their body weight 200-250 kg, in addition to 15 unthrifty calves and their body weight 120-150 kg) belonged to a private farm in Abo Hamad City - Sharkia Province. All calves were injected with one dose of Ivomec super to ensure that all calves were free from internal and external parasite. At day 30 post Ivomec injection, calves were divided into 4 equal groups (5/each). All calves were received 3 kg concentrates contain 2 kg mineral mixture/ton and 5 kg berseem all over the experimental period. The1st group healthy buffalo calves (-ve control), the 2nd group unthrifty calves not treated (+ve control), the 3rd group unthrifty calves received 0.5 ml synbiotics /I liter drinking water for 60 successive days, the 4th group unthrifty calves received 1 % dried leaves of damsisa for 60 days. All caves were weighted at start of the experiment and at 1st day post treatment for determination weight gain and FCR. At1st day post treatment blood samples were taken from all calves for estimation some hematobiochemical parameters. Unthrifty calves show significant decrease in serum copper, iron, selenium, zinc, body weight gain, RBCs, Hb, PCV, WBCs, serum total protein albumin, globulin, A/G ratio cholesterol, CAT and SOD beside insignificant decrease in Ca, Ph, Na, FCR, total lipid, triglycerides and MDA associated with non-significant increase in AST, ALT, ALP. Findings revealed that unthrifty calves received synbiotics  or damsisa for 60 successive days displayed significant increases in weight gain, total protein, albumin, globulin, A/G ratio, beside insignificant increases in copper, iron, selenium, zinc, Ca, Ph, Na, RBCs, Hb, PCV, WBCs, AST, ALT, ALP, Total lipid, triglyceride, MDA coupled with non-significant decreases in RBCs, Hb, PCV and WBCs, serum cholesterol, CAT and SOD. It could be concluded that unthriftiness induce many adverse effects in health and body performance of calves but mineral mixture with synbiotics or with damsisa had better results in improvement the adverse effects in hematobiochemical parameter in unthrifty calves.

This study aimed at investigation of the prevalence and antimicrobial susceptibility of Pseudomonas spp., in two marine water fish, namely Pagrus, and saurus intended for human consumption. In the current study, Pseudomonas spp., was isolated and identified from two marine water fishes. Fifty Pagrus fish and 50 Saurus (Saurida undosquamis) fish were sampled from fish markets at Sharkia governorate, Egypt. The prevalence rates of Pseudomonas spp. were 84%, and 40% in Pagrus fish, and Saurus respectively. The identified Pseudomonas spp., from Saurus was Pseudomonas aeruginosa (P.  aeruginosa), P. fluorescens, P. fragi, P. cepacia, at 20% for each. P. alcaligenes and P. lundensis were recovered at 10%. In Pagrus, the prevalence rates were 31.3%, 56.3%, and 6.2% for P. aeruginosa, P. fluorescens, P. fragi and P. stutzeri. Seven serotypes of P. aeruginosa were identified with the serotypes O11 at the top prevalence (42.8%), and O6, O5, O1, O8 at 19.3% for each. The virulence-associated genes were lasB (Elastase B gene), exoS (Exoenzyme S) and pilB (pili gene) were detected in the recovered P. aeruginosa at 100%, 71.4%, and 28.6%, respectively.  The recovered Pseudomonas species had high antimicrobial resistance to erythromycin, amoxicillin, ampicillin and gentamycin at 100%, 88.5%, 65.4%, and 50%, respectively. In conclusion, Pagrus and Saurus fish species are considered as potential sources of multidrug resistant Pseudomonas spp.

In several nations of the Middle East, camel meat is a significant source of animal-derived protein, vitamins, and minerals. In the Arab and African continents, camels are extremely significant. The study was conducted to determine the residual levels of heavy metals (lead, cadmium, nickel, and chromium). Camel meat plays a significant role in these regions as a source of protein, but it may also contain many toxic substances as residues contamination with heavy metals, which are considered serious threats not only due to their toxicity but also because of bioaccumulation in the food chain. The average lead levels in the analyzed samples of muscles, kidneys, and livers were 0.11±0.03, 0.47±1.18, and 0.7±0.13 mg/kg, respectively. The mean concentrations of cadmium in muscles, livers, and kidneys, however, were 0.13±0.04, 0.43±0.12, and 0.85±0.34 mg/kg, respectively. While the residual chromium levels in the muscles, livers, and kidneys were, respectively, 0.1±0.45, 0.21±0.11, and 0.25±0.07 mg/kg. The residual level of all the metals in different tissues were found to be substantially different, with a p-value <0.05, whereas nickel was 0.13, 0.16, and 0.23 mg/kg. In conclusion, liver and kidneys has higher concentrations of the tested metals compared with muscles. However, the recorded concentrations of the tested metals lies within the acceptable limits set by the regulatory authorities.

Despite of the great efforts to develop effective control programs for mastitis, it is still one of the most economically important diseases in dairy cattle herds. Pseudomonas aeruginosa is a member of coliform Gram-negative bacteria causing treatment-resistant clinical or sub-clinical mastitis in dairy cows. This study aimed to evaluate the influence of affection with clinical P. aeruginosa mastitis on some oxidative stress biomarkers, inflammatory cytokines and proteins, in addition to some complement factors in Holstein dairy cows. Affection with P. aeruginosa mastitis evoked a state of oxidative stress which accompanied with depletion of cellular enzymatic and non-enzymatic anti-oxidants and elevation of lipid peroxide and advanced oxidation protein product (AOPP) level. Additionally, this affection stimulated the release of some inflammatory cytokines and proteins, enhanced activity of caspase-1. In contrary, the level of complement factor 2 (C2), complement fragments C3b and complement fragment C5a has been decreased upon affection with mastitis. In conclusion, marked oxidative stress state and enhanced release of inflammatory cytokines and proteins with complement system defective activation may share in pathogenesis and virulence of P. aeruginosa-induced clinical mastitis in dairy cattle.

Bacterial diseases are one of the most challenging issues facing aquaculture sector. Pseudomonas aeruginosa (P. aeruginosa) has been regarded as one of the most significant threats to the fishing industry, which also affects public health. We aimed to elucidate the occurrence and antibiogram profile of P. aeruginosa recovered from Nile tilapia (Oreochromis niloticus) and smoked herring (Clupea harengus) with emphasis on their antibiotic resistance genes (blaTEM, blaSHV, blaOXA-1 and ampC) and virulence determinant genes (oprL and toxA). A total of 150 fish samples (110 diseased Nile tilapia, and 40 smoked herring) were collected randomly from retails of Gharbia Governorate, Egypt. The retrieved isolates were phenotypically characterized using standard methods of culturing and biochemical tests. Then, verified using molecular assay, 16S rRNA gene was detected in 100% of the tested isolates. The overall incidence of P. aeruginosa was 33.3%, out of which 45% from smoked herring and 29% from Nile Tilapia. The occurrence of P. aeruginosa in various infected organs of O. niloticus showed that the gills were the most obviously infected organ followed by kidney, liver, and spleen, respectively. A significant difference (P< 0.05) was noticed in the distribution of P. aeruginosa among O. niloticus internal organs. The phenotypic susceptibility to nine commonly used antimicrobial agents was detected using disc diffusion assay. The tested strains were extremely susceptible to ciprofloxacin, amikacin, and imipenem, whereas exhibited remarkable resistance to oxacillin, cefpodoxime, amoxicillin with clavulanic acid, ceftriaxone, and nalidixic acid. Interestingly, 100% of P. aeruginosa isolates were multiple antimicrobial resistant (MAR). Three resistance phenotypes profiles were identified with MAR index ranged from 0.4-0.5. Screening for antibiotic resistance genes revealed a diversity of β-lactamases in P. aeruginosa isolates, with blaTEM being the most dominant gene (100%), followed by blaSHV, blaOXA-1 and ampC with a total prevalence of 66.6% to all of them. The identified antimicrobial resistance phenotypes and genotypes were found to be significantly correlated. Subsequently, the distribution of virulence determinants in these strains was identified. These isolates had 100% prevalence of oprL and toxA virulence genes. In conclusion, the emergence of MDR P. aeruginosa in fish particularly ESBL and AmpC beta-lactamases producers could pose a potential health hazard to consumers. Thus, antimicrobial susceptibility must be continuously monitored to assess potential risks to human health. Ciprofloxacin, amikacin, and imipenem were the most efficient antibiotics for treatment of the identified P. aeruginosa, ESBL and AmpC beta-lactamases producers.

Emergence of antimicrobial resistance (AMR) among fish bacterial pathogens is one of the major global public threats. Attempts are being made to develop novel alternatives as a promising approach to combat multidrug resistance disease-causing bacteria. Natural antimicrobials such as essential oils (EOs) are a potential unique strategy to treat bacterial infections with a reduced risk of resistance developing. This study aimed to evaluate the antimicrobial activity of some essential oils (EOs) namely, cinnamon (Cinnamomum zeylanicum), clove (Syzygium aromaticum), Peppermint (Mentha piperita) , and black cumin (Nigella sativa) against some fish pathogens implicated with aquaculture disease outbreaks like Aeromonas hydrophilia, Pseudomonas fluorescence, Photobacterium damselae and Streptococcus agalactiae using agar well diffusion assay. We found significant differences on the antibacterial activity depending on the type of essential oils and bacterial strain. Among all the tested EOs, cinnamon essential oil (CEO) was shown to be the most effective with minimum bactericidal concentration (MBC) ranged from 0.0156-0.125 ml/ml. As a result, it was selected for our in vivo investigations. We next aimed to investigate the effects of dietary CEO on growth performance, disease resistance and immune response of fish. A total of 150 striped catfish (Pangasianodon hypophthalmus) were fed with different levels of CEO (0, 1.50, 2.0, 2.50, and 3.0 mL/kg diets) (assigned as control, Diet 1, Diet 2, Diet 3 and Diet 4) for 60 days. Compared to control, fish fed with graded levels of dietary CEO showed significant (P < 0.05) increase in final body weight, weight gain %, and specific growth rate particularly at fish group fed diet 3. Of interest, there were no significant differences (P > 0.05) in feed conversion ratio and survival rates among control and CEO-supplemented groups. Moreover, we found significant (P < 0.05) increases in plasma lysozyme activity and total IgM levels in a dose dependent manner with dietary CEO supplementation. After feeding trials, we investigated their potential to defend striped catfish against A. hydrophila challenge. Fish fed control diet had the highest mortality rates; in contrast, fish fed diets supplemented with CEO had higher levels of resistance to the bacterial infection, with the lowest mortality rates in the fish group fed diet 3. Overall, these findings showed that EOs exhibit a great potential to be used as antimicrobial agents against fish pathogens. Moreover, dietary administration of CEO, particularly at 2.5 ml/kg feed, can be regarded as a promising component for improving growth, immunological responses and potential alternatives to conventional antimicrobials for control of microbial infections in fish.

Staphylococcus aureus (S. aureus) is considered the most important cause of food borne intoxication, which occurs due to ingesting of food contaminated with enterotoxin of S. aureus. In this study, S. aureus from saurus, and pagrus fish species were isolated, then identified by morphological and biochemical examination. S. aureus coagulase, and D-Nase +ve were detected in 16 from 25 (64%), and 17 from 25(68%) of the examined samples of saurus, and pagrus, respectively. Total S. aureus counts were 5.14±0.06 in saurus, and 5.02±0.03 log 10 cfu/g in pagrus fish, respectively. Detection and typing of enterotoxin by Reverse Passive Latex Agglutination technique "RPLA" revealed the detection of staphylococcal enterotoxins (SEs) SEA, SEC, and SED at 18%, 6%, 0% in saurus and 5%, 0%, and 11%, at pagrus, respectively. S. aureus was tested for antimicrobial susceptibility. The recovered S. aureus coagulase, and D-Nase +ve in the current study showed resistance to kanamycin, clindamycin, nalidixic acid, and sulphamethoxazole at 100%, 87.9%, 84.8%, and 81.8%, respectively. The isolates showed sensitivity to amikacin, imipenem, meropenem, oxacillin at 90.9%, 87.9%, 84.8%, and 81.8% respectively. It could be concluded that multidrug resistant enterotoxigenic strains of S. aureus could be isolated from saurus and pagrus spp. Therefore, it is highly recommended to adopt strict hygienic measures and efficient cooking during before consumption of such fish species.

Meat contamination is a problem that must be addressed since it can affect consumers' health. Standard acceptable limits for heavy metals in meat and organs have been established by many international organizations. Livestock including sheep, cattle, camels, and goats side by side with other poultry species are the most significant sources of meat for protein. Heavy metal contamination in animal products may be harmful to people's health. The earth's crust naturally contains heavy metals, but uncontrolled human activity has significantly changed their geochemical cycles and biological equilibrium. This causes a buildup of metals in animal tissue that contain secondary metabolites, which gives rise to a specific pharmacological action. Humans can have negative health effects from prolonged exposure to heavy metals as lead, cadmium, arsenic, and mercury. This review showed that most of the studies conducted in different countries on heavy metals were mostly in red meat, and the research was few in the cases of domesticated birds and very rare in game birds, although it is considered a good example for investigating heavy metals in the environment