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Effect of selected nonsteroidal anti-inflammatory drugs on the viability of canine osteosarcoma cells of the D-17 line: in vitro studies Texte intégral
2019
Poradowski, Dominik | Obmińska-Mrukowicz, Bożena
Effect of selected nonsteroidal anti-inflammatory drugs on the viability of canine osteosarcoma cells of the D-17 line: in vitro studies Texte intégral
2019
Poradowski, Dominik | Obmińska-Mrukowicz, Bożena
Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in veterinary medicine. They are used in pain control and in anti-inflammatory and antipyretic therapies. Some NSAIDs, e.g piroxicam, also have a documented anticancer effect. The objective of this study was to evaluate which of the commonly used NSAIDs (etodolac, flunixin, tolfenamic acid, carprofen, and ketoprofen) are cytotoxic to the D-17 cell line of canine osteosarcoma. The viability of the cells was evaluated using the MTT assay. Four independent repetitions were performed and the results are given as the average of these values; EC₅₀ values (half maximal effective concentration) were also calculated. The analysis of results showed that carprofen and tolfenamic acid displayed the highest cytotoxicity. Other drugs either did not provide such effects or they were very poor. For carprofen, it was possible to determine an EC₅₀ which fell within the limits of concentrations obtainable in canine serum after the administration of routinely used doses. The results are promising but further studies should be conducted to confirm them, since this study is only preliminary. The possibility of introducing carprofen and tolfenamic acid into the routine treatment of osteosarcoma in dogs should be considered.
Afficher plus [+] Moins [-]Effect of selected nonsteroidal anti-inflammatory drugs on the viability of canine osteosarcoma cells of the D-17 line: in vitro studies Texte intégral
2019
Poradowski Dominik | Obmińska-Mrukowicz Bożena
Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in veterinary medicine. They are used in pain control and in anti-inflammatory and antipyretic therapies. Some NSAIDs, e.g piroxicam, also have a documented anticancer effect. The objective of this study was to evaluate which of the commonly used NSAIDs (etodolac, flunixin, tolfenamic acid, carprofen, and ketoprofen) are cytotoxic to the D-17 cell line of canine osteosarcoma.
Afficher plus [+] Moins [-]Determination of steroid esters in hair of slaughter animals by liquid chromatography with tandem mass spectrometry Texte intégral
2019
Matraszek-Żuchowska, Iwona | Woźniak-Sobczak, Barbara | Sielska, Katarzyna | Posyniak, Andrzej
Determination of steroid esters in hair of slaughter animals by liquid chromatography with tandem mass spectrometry Texte intégral
2019
Matraszek-Żuchowska, Iwona | Woźniak-Sobczak, Barbara | Sielska, Katarzyna | Posyniak, Andrzej
The use of growth promoters in animal husbandry to increase weight gain and efficiency of feed conversion into muscle has been banned in the European Union since 1988, and under Directive 96/23/EC, surveillance for anabolic steroid hormones is obligatory. The hormones present in animal tissues may be of endogenous origin or may result from illegal administration. Steps have been taken to determine selected steroids in the form of esters in the alternative matrix of animal hair. Their detection in biological material is direct proof of the illegal use of anabolics. The procedure for the determination of steroid esters in animal hair, based on digestion, extraction, purification, and liquid chromatography with tandem mass spectrometry was validated under the current regulations. In total, 348 samples of animal hair were examined using this method. Good recoveries and precision values (RSD) were obtained during validation. Decision limits (CCα) and detection capabilities (CCβ) were in the ranges of 2.57–4.18 μg kg⁻¹ and 4.38–7.12 μg kg⁻¹, respectively. The method met the criteria for confirmation techniques with respect to Commission Decision 2002/657/EC. Testing for steroid esters in animal hair was introduced into the National Residue Control Programme in 2017. Steroid esters were not found in any hair samples above the CCα, which indicates that illegal use of anabolics was not confirmed.
Afficher plus [+] Moins [-]Determination of steroid esters in hair of slaughter animals by liquid chromatography with tandem mass spectrometry Texte intégral
2019
Matraszek-Żuchowska Iwona | Woźniak Barbara | Sielska Katarzyna | Posyniak Andrzej
The use of growth promoters in animal husbandry to increase weight gain and efficiency of feed conversion into muscle has been banned in the European Union since 1988, and under Directive 96/23/EC, surveillance for anabolic steroid hormones is obligatory. The hormones present in animal tissues may be of endogenous origin or may result from illegal administration. Steps have been taken to determine selected steroids in the form of esters in the alternative matrix of animal hair. Their detection in biological material is direct proof of the illegal use of anabolics.
Afficher plus [+] Moins [-]Presence of tapeworms (Cestoda) in red fox (Vulpes vulpes) in north-western Poland, with particular emphasis on Echinococcus multilocularis Texte intégral
2019
Tylkowska, Agnieszka | Pilarczyk, Bogumiła | Pilarczyk, Renata | Zyśko, Michał | Tomza-Marciniak, Agnieszka
Presence of tapeworms (Cestoda) in red fox (Vulpes vulpes) in north-western Poland, with particular emphasis on Echinococcus multilocularis Texte intégral
2019
Tylkowska, Agnieszka | Pilarczyk, Bogumiła | Pilarczyk, Renata | Zyśko, Michał | Tomza-Marciniak, Agnieszka
Introduction: Foxes are a reservoir of parasites that are dangerous to humans. The aim of the study was to determine the parameters associated with the occurrence of tapeworms in red foxes in north-western Poland. Material and Methods: Parasitological sections were taken from 620 red foxes using IST and SCT methods in 18 districts of West Pomerania Province. Results: The extensity of fox infection with tapeworms was 61%. Echinococcus multilocularis, Mesocestoides spp., Dipylidium caninum, and specimens of the genus Taenia were identified. E. multilocularis was found in 11 districts. Mesocestoides spp. demonstrated the highest prevalence (41.3%), while E. multilocularis demonstrated the lowest prevalence (2.9%); however, it infected foxes with the greatest mean intensity (235.6 tapeworms per fox). The most common co-occurrence in a single host organism was observed for Mesocestoides spp. and tapeworms of the genus Taenia; however, no examples were found of coinfection by E. multilocularis and D. caninum. Conclusion: The occurrence of tapeworms in foxes was high in West Pomerania Province and was often higher than observed in previous years. For this reason, the risk of parasite transmission to humans and domestic animals is mounting. The risk of infection is also amplifying due to the growth of the fox population.
Afficher plus [+] Moins [-]Presence of tapeworms (Cestoda) in red fox (Vulpes vulpes) in north-western Poland, with particular emphasis on Echinococcus multilocularis Texte intégral
2019
Tylkowska Agnieszka | Pilarczyk Bogumiła | Pilarczyk Renata | Zyśko Michał | Tomza-Marciniak Agnieszka
Introduction: Foxes are a reservoir of parasites that are dangerous to humans. The aim of the study was to determine the parameters associated with the occurrence of tapeworms in red foxes in north-western Poland.
Afficher plus [+] Moins [-]Evaluation of the possibility of C. burnetii transmission by the alimentary route in a guinea pig model Texte intégral
2019
Jodełko, Agnieszka | Szymańska-Czerwińska, Monika | Kycko, Anna | Niemczuk, Krzysztof
Evaluation of the possibility of C. burnetii transmission by the alimentary route in a guinea pig model Texte intégral
2019
Jodełko, Agnieszka | Szymańska-Czerwińska, Monika | Kycko, Anna | Niemczuk, Krzysztof
Q fever (coxiellosis) is an infectious disease of animals and humans, caused by.C. burnetii and widely distributed throughout the world. It is known that people and animals acquire the disease predominantly.via inhalation of infectious aerosols. The possibility of transmission of the pathogen by the alimentary route is still a matter of debate and remains controversial. Therefore the aim of this study was to fill the gaps in knowledge of oral transmission of.C. burnetii by conducting biological tests on the guinea pig model. Guinea pigs, divided into five groups comprising a negative control and four experimental groups, received specified concentrations of.C. burnetii per os. To determine the presence of specific antibodies, blood samples were tested using CFT. Also, internal organs collected during necropsy were screened by a real-time PCR targeting I.1111. Additionally, histopathological evaluation of the tissues was performed. The presence of antibodies and pathogen DNA in caecum was confirmed in one guinea pig from experimental group IV..C. burnetii was also detected in testicular tissue collected from one animal of experimental group II. The presence of pathogen DNA in the testicular tissue indicates that infection spreads haematogenously. In the majority of experimental animals specific antibodies and genetic material of.C. burnetii were not detected. This fact suggests that development of infection depends on many factors, such as animal immune status.
Afficher plus [+] Moins [-]Evaluation of the possibility of C. burnetii transmission by the alimentary route in a guinea pig model Texte intégral
2019
Jodełko Agnieszka | Szymańska-Czerwińska Monika | Kycko Anna | Niemczuk Krzysztof
Q fever (coxiellosis) is an infectious disease of animals and humans, caused by.C. burnetii and widely distributed throughout the world. It is known that people and animals acquire the disease predominantly.via inhalation of infectious aerosols. The possibility of transmission of the pathogen by the alimentary route is still a matter of debate and remains controversial. Therefore the aim of this study was to fill the gaps in knowledge of oral transmission of.C. burnetii by conducting biological tests on the guinea pig model.
Afficher plus [+] Moins [-]Improving The Reporting Of Observational Studies In Veterinary Research: Turkish Adaptation Of Strobe-Vet Criteria Texte intégral
2019
Tekindal, Mustafa Agah | Balak, Tuba | Günay, Feyza | Kaymaz, Özlem | Çevrimli, Mustafa Bahadır
This study, the Turkish version of the STROBE-VET was made to strengthen the reporting of observational studies in the veterinary fieldand making the usage of the STROBE-VET better understood by the researchers. Firstly, STROBE-VET expression translated into Turkishby 4 authors (TB, FG, ÖK, MAT) each from the other and afterwards four translated versions have been combined with consensus by experttranslator. The final version of criteria was translated into English by another expert translator using reverse translation method and found to bequiet similar between translated of Turkish control list and the original control list. Secondly, in order to assess the Turkish control list whetherit is understood correctly by the researchers, 20 studies in the field of veterinary sciences have been evaluated by two veterinarians (DÖ andMBC). The correspondence between the responses of the researchers was examined using Cohen's Kappa statistic ( ) and the percentage ofcompliance between researchers for each criterion. In addition, it was assessed using the Bland-Altman Method and the Intraclass CorrelationCoefficient (ICC) using the total number of reported criteria. According to results, criteria has a great variation in the frequency of reportingin the studies. The Turkish version of STROBE-VET will be a guide for the referees and editors of journals as well as researchers studying inthe field of veterinary science.
Afficher plus [+] Moins [-]Intercellular adhesion molecule-1 (ICAM-1) and C-Reactive Protein (CRP) levels in Calves Naturally infected with Mycoplasma bovis Texte intégral
2019
Kozat, Süleyman | Özkan, Cumali
Mycoplasma bovis is one of the most important pathogens causing pneumonia worldwide. In this study, it was aimed to determine intercellular adhesion molecule-1 (ICAM-1) and C-reactive protein (CRP) levels in calves infected with Mycoplasma bovis. The animal material of the study consisted of 30 calves including 20 simmental calves with pneumonia due to Mycoplasma bovis at 2-4 months of age and 10 healthy calves at the same age and the race. Serum levels of intercellular adhesion molecule-1 (ICAM-1) and C-reactive protein (CRP) were determined in calves infected M. bovis and control group. Serum levels of intercellular adhesion molecule-1 (ICAM-1) and C-reactive protein (CRP) were found to be statistically higher in the calves with pneumonia than in the control group (P<0.01). A positive correlation between serum ICAM-1 concentrations and Mycoplasma bovis titers (increases in coefficient ratios) were statistically significant (r=0.664), indicating a positive correlation between serum ICAM-1 and CRP levels and titers of Mycoplasma bovis in the calves with pneumonia. As a result, it was observed that serum intercellular adhesion molecule-1 (ICAM-1) and C-Reactive Protein (CRP) concentrations were increased in infected pneumonia with Mycoplasma bovis. It is considered useful to consider these parameters in evaluating the prognosis of pneumonia cases of Mycoplasma bovis origin and the efficacy of applied treatment.
Afficher plus [+] Moins [-]Possibilities Of Estimating Body Weight From Different Body Measurements In Hair Goat Using Different Regression Models Texte intégral
2019
Demiraslan, Yasin | Gurbuz, Iftar | Gurkan Dilek, Omer | Ozudogru, Sukru | Ozgel, Ozcan
In the study, the data obtained to describe the body characteristics of the Hairpin were utilized in the businesses that were registered with Karaman Province Breeding Sheep Goat Breeders Association. Body weights of 130 goats, 2, 3, 4, 5, 6 and 7 years old and 50 goats, 2, 3 and 4 years old, selected by simple random sampling method were used in the data of total 900. In the study, Pearson correlation coefficient for variables providing parametric test prerequisites, and Spearman correlation analysis for variables not providing parametric test prerequisites. In the regression analysis, "live weight" dependent variable and other variables were determined as independent variables and parametric and nonparametric regression methods were applied. Univariate and multivariable regression models were applied for the whole data set. When all analyzes are evaluated, univariate regression models give lower determination coefficients (R2) than multivariate models. In this case, it has been deemed appropriate to use a multivariate regression model instead of a univariate model in order to make a correct prediction.However, in practice, univariate Quadratic or Cubic regression methods can be used for researchers.
Afficher plus [+] Moins [-]Survival and Acid Tolerance of Shigatoxigenic Escherichia coli (STEC) During Frankfurter Sausage Storage Texte intégral
2019
Bozatlı, S. Betül | Dikici, Abdullah
This study was carried out to understand how sausage matrices affect the survival and acid tolerance of STEC O157 and O26. STEC O157 and O26 were inoculated on sausage surface approximately 5 log. After inoculation sausages were vacuum packed and stored at 4°C. Pathogen counts and synthetic gastric fluid (pH 1,5) experiments were conducted on day 0, 15 and 30 of the storage. Three trials were conducted for each pathogen separately. Both serogroups had viable counts on sausage during storage, STEC O26 count decreased about 1log and O157 about 3log during storage. At the end of the storage both O26 and O157 were viable on the sausage surface 4.59 log and 2.54 log respectively. For acid survival experiments pathogen counts were obtained on 30th, 60th and 90th minute of synthetic gastric fluid (SGF) exposure. Our results show that O26 endured acid stress longer than O157 during SGF experiments throughout storage of frankfurters. The results of this study may support the idea that some non-O157 STEC strains might be more resistant to acid stress than O157 STEC but further studies should be conducted before drawing a conclusion.
Afficher plus [+] Moins [-]Çıldır Gölü'ndeki̇ Kerevi̇t (Astacus leptodactylus Eschscholtz, 1823) Avcılığının Sosyal, Yapısal Ve Ekonomi̇k Yönden Değerlendi̇ri̇lmesi Texte intégral
2019
KOÇYİĞİT, Ahmet | AKSU, Önder
This study was carried out to investigate the socio-economic analysis of fishermen of Çıldır Lake (Ardahan/Kars) cooperative. It has beendetermined that all fishermen are members of these cooperatives and that they are not members of any other nongovernmental organization. Ithas been determined that the ages of fishermen vary between 34 and 74, 100% are married and their spouses are between 20 and 70 years old.They were found to be primary school graduates with a rate of 74%. 65.21% of fishermen received a special training on their professions. Itwas found that 17.4% of fishermen had no sources of income other than fishing, and the remaining 82.6% were active in agriculture, animalhusbandry or other fields. It is generally determined that they have 1 boat, the majority of the boats are 6 m in length and made of iron. It wasseen that 43.47% of family members participated in hunting activities. Ownership of all of the boats belonged to the fishermen themselves and30.4% of them were informed that they were receiving them with the help of their boat families. It was found that fishermen used pinters incrayfish hunting and fennel nets in fish hunting. Although Çıldır Lake fishermen 's professions are found to be economically inadequate, it hasbeen determined that 14 fishermen with 60.87% of their income are happy to do this job due to their presence in one or more of the reasons foradditional income, habit, passion / hobby.
Afficher plus [+] Moins [-]The Scrutinising of Changes in the Aquaculture Supports Texte intégral
2019
DANABAS, Durali
In the world, aquaculture is seen as the fastest growing and developing food production sector and however, in the near future, it is estimatedthat the rates of fisheries and aquaculture will be equalized. In 2015, the fisheries production of the world amounted to 170,345,641 tons (fisheries;93,704,616 tons and aquaculture; 76,641,025 tons) according to the FAO records.The aquaculture in Turkey started in 1970s and its rate in total fisheries production has rapidly increased as of 2000. In our country, the amountof aquaculture, which was 79,943 tons in 2003, reached 253,395 tons in 2016. The aquaculture rate in the total production of our country hasincreased by more than 310%. As one of the most important reasons for this rate increase, it is seen to be providing some support policies withvarious incentive systems by the government for support and encourage the aquaculture in country.Directly product support for aquaculture farmers was started in 2003. According to the total capacity of the farms, total product and fry supportswere provided. In 2008, the total capacity supported was limited to 2000 tons/year. However, in 2012, different tonnage application (whole unitprice to 250 tons/year and ½ unit price from 251 to 500 tons/year) were brought to the total product support and the fry support was removed.At the same time, the processed product support was brought at the rate of twice the amount specified in the invoices of the total product. In thepresent, the support system is still continuing.The types of product support, prices, supported capacities and the expense criteria are applied annually by issuing the “Aquaculture SupportCommuniqués” in the Official Gazette.
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