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OLFACTOMETRY BIOASSAY STUDIES ON BEHAVIOUR OF HOUSE FLIES TOWARDS DIFFERENT FOOD BAITS IN VITRO Texte intégral
2025
S.T. Bino Sundar | T.J. Harikrishnan | Bhaskaran Ravi Latha | T.M.A. Senthil Kumar | G. Sarath Chandra | A. Serma Saravana Pandian | C. Pandian | K. Ambasankar
Food bait preferences of house flies were evaluated using olfactometry bioassays. A single cage dual port glass olfactometer was used to assess preferences of house flies towards sugar, fish meal and molasses in no choice and choice bioassays. Flies were allowed into olfactometer individually, observed for five minutes. Time spent in bait port and decision chamber, number of entries into test/control port and port selected five minutes post-exposure were recorded. In the second study in a four arm acrylic olfactometer, three food baits viz., high fish meal low molasses cake, low fish meal high molasses cake and mango essence were tested In the glass olfactometer, flies showed positive response towards food baits compared to control in no choice bioassay. In choice bioassay, flies preferred fish meal than both sugar and molasses and molasses was preferred over sugar. In four arm acrylic olfactometer bioassay, 43.87, 30.96 and 25.16 per cent flies were attracted to high fish meal low molasses cake, low fish meal high molasses cake and mango essence, respectively. Results revealed that molasses and fish meal based baits can be used in housefly traps. Olfactometry bioassays were found effective in bait preference studies of house flies.
Afficher plus [+] Moins [-]MOLECULAR CHARACTERIZATION, BIOFILM FORMATION AND ANTIBIOGRAM ANALYSIS OF ENTEROTOXIGENIC BACILUS CEREUS FROM MILK AND MILK PRODUCTS IN PUDUCHERRY Texte intégral
2025
S. Sathiesh | V. Bhanu Rekha | V. J. Ajay Kumar | J. Thanislass | R. Sivachandiran
The present investigation was undertaken to study the occurrence of enterotoxigenic B. cereus from 200 samples including 100 raw milk, 50pasteurized milk and 50 ice cream samples sold in Puducherry. The results revealed that 68 samples (34 %) belonged to B. cereus group which included 36 from raw milk (36%), 9 from pasteurized milk (18%) and 23 from ice cream samples (46 %). On PCR analysis, 64 out of 68 isolates showed the presence of gyrB gene which differentiates B. cereus from B. cereus group isolates. Multiplex PCR revealed the presence of four enterotoxigenic genes hblA, nheA, cytK and entF Min 32.35 %, 77.94 %>, 54.41 % and 94.12% of the 64 isolates. On biofilm production assay, out of 64 enterotoxigenic B. cereus isolates, 60 (93.75 %) isolates showed the ability to form biofilm in which 13 (20.31 %) were strong, 21 (32.81 %) were moderate and26 (40.63 %) were weak biofilm producers. All the isolates were resistant to ampicillin and penicillin G and sensitive to ciprofloxacin, gentamicin, vancomycin, streptomycin and norfloxacin. The current study directs the need for proper hygienic measures during collection, production, processing and storage of milk to reduce the contamination level. It also indicates the need for surveillance and action to reduce the contamination of enterotoxigenic B. cereus in milk and milk products in Puducherry.
Afficher plus [+] Moins [-]FUNCTIONAL CHICKEN NUGGETS ENRICHED WITH DIETARY FIBRE FROM KODO MILLET-AN INDIAN NUTRI CEREAL Texte intégral
2025
C. Vasanthi | V. Appa Rao | R. Narendra Babu | R. Karunakaran | S. Ezhilvelan | R. Ramani | M. Muthulakshmi
The optimization of Kodo millet (Paspalum scrobiculatum) as a healthy alternative to refined wheat flour (RWF) in chicken nuggets was investigated to assess dietary fibre enrichment. Replacement was performed at four levels: 5% (Tl), 6% (T2), 7% (T3), and 10% (T4), while control nuggets were prepared using RWF. Product quality, texture profile, sensory evaluation, and dietary fibre content were assessed. Emulsion stability and product yield remained statistically similar, with improved textural characteristics observed up to 7% replacement with Kodo millet. The product's acceptance was highly significant (P<0.01) at 7% inclusion (7.61±0.32), with dietary fibre enrichment showing a 126.49% increase over the control. The findings concluded that 7% Kodo millet flour inclusion is the optimal level for dietary fibre enhancement in functional chicken meat nuggets.
Afficher plus [+] Moins [-]METHOD DEVELOPMENT AND VALIDATION OF RICINOLEIC ACID BY HPTLC Texte intégral
2025
Raghu Duraisamy | Parthasarathy Mahadevan | Baskaran Siva | Ramesh Jaya Rama Chandra Reddy | Gadevaru Sarathchandra
Ricinoleic acid, a toxic compound found in castor seeds (Ricinus communis L.), can be lethal when inhaled, ingested, or injected at doses of 5-10 pg/kgto human or animals. This study presents a validated, rapid, and precise high-performance thin-layer chromatographic (HPTLC) methodfor its quantification. Separation was achieved on silica gel 60 F254plates using a mobile phase oftoluene, ethyl acetate and acetate acid (6:4:0.2 v/v/v). Densitometric scanning at 450 nm detected ricinoleic acid with an Rfvalue of 0.66 (±0.04). Linearity was observed in the range of 20-100 ng/spot with R = 0.998 ± 2.62%.
Afficher plus [+] Moins [-]CONCURRENT INFECTION OF MONIEZIA EXPANSA AND HAEMONCHUS CONTORTUS IN GOATS AND ITS THERAPEUTIC MANAGEMENT Texte intégral
2025
G. Manojkumar | S. Yogeshpriya | A. Latchumikanthan | N. Premalatha | R. Velusamy
Goats are highly susceptible to endoparasitic infections due to free grazing habits. The present study was reported for concurrent infection ofMoniezia expansa and Haemonchus contortus in two goats from a flock of 40 goats in Orathanadu, Thanjavur. History included anorexia, foetid diarrhoea and jowl edema in goats. On clinical examination, pyrexia, pale conjunctival mucous membrane, pasty diarrhoeic faeces, bottle jaw and abdominal thudding were noticed. Coproscopy revealed Moniezia expansa eggs and strongyle eggs and faecal culture revealed Haemonchus contortus larvae. Uneventful recovery was noticed in goats after treating with praziquantel and fenbendazole for 3 days with supportive therapy.
Afficher plus [+] Moins [-]SUCCESSFUL MANAGEMENT OF CANINE ATOPIC DERMATITIS USING OCLACITINIB - A CASE REPORT Texte intégral
2025
Navjot Singh Thakur | M.A. Ensha Lomiya | R. Raguvaran | Umesh Dimri | Vivek Joshi | D.B. Mondal | K. Kavitha | Akhilesh Kumar
A Dalmatian dog -was brought to the Referral Veterinary Polyclinic, ICAR- Indian Veterinary Research Institute, Izatnagar with a complaint of erythematous lesions, alopecia and intense itching and had been treated for a long time. Atopic dermatitis was diagnosed based on the history and the exclusion of other infectious agents as potential causes of the dermatitis. The dog was treated with oclacitinib, antibacterial, fatty acids and antihistaminic medications and showed remarkable improvement after therapy.
Afficher plus [+] Moins [-]TREATMENT OF AMITRAZ TOXICITY IN A DOG: A CASE REPORT Texte intégral
2025
B.K. Bhagya | V. Shivakumar | M. Shivakumar
A one and half year, female, non descriptive dog was presented to Veterinary College Hospital, Hassan with the history of incoordination, drowsiness and vomiting after accidental oral administration ofAmitraz liquid (RIDD®) by the owner. On physical examination animal was dull and depressed. Sedation (drowsiness) and hypothermia was noticed with the rectal temperature of 98.8°E Hemato-biochemical findings were within the normal range. Based on history and clinical signs, the case was diagnosed as amitrazpoisoning and animal was treated with Yohimbine Hydrochloride at the rate of 0.2 mg per Kg body weight intramuscularly and activated charcoal per orally, along with the supportive therapy. Animal was completely recovered after 5 days of treatment.
Afficher plus [+] Moins [-]INCOMPLETE CERVICAL DILATATION WITH UTERINE ADHESION AND ITS SURGICAL MANAGEMENT IN A NON-DESCRIPT DOE Texte intégral
2025
T. Sarath | S. Pravina | R. Rajkumar | A. Thangamani | A. Reshma | S. Manokaran
A case of dystocia due to incomplete cervical dilation consequent to uterine adhesion in non-descript doe and its surgical management was reported in this paper.
Afficher plus [+] Moins [-]DEVELOPMENT OF PROTEIN ENRICHED GLUTEN FREE KODO MILLET MUFFINS INCORPORATED WITH CHICKEN MEAT POWDER Texte intégral
2025
S. Madesh | P. Sivakumar | Rita Narayanan | V. Nithyalakshmi
The increasing demand for nutritious, gluten-free food options necessitates the development of innovative products. Many commercially available millet-based products fail to leverage the nutritional benefits of millets, incorporating them in minimal quantities while maintaining refined wheat flour (maida) as the primary ingredient in baked goods. This research aims to develop a Kodo millet muffin formulated entirely from Kodo millet flour and enriched with animal protein through the addition of chicken meat powder (CMP). The CMP was prepared and its inclusion level was optimized by varying from 0% to 20% in increments of 5%, based on sensory evaluation using a 9-point hedonic scale. The formulation containing 10% CMP was identified as the most acceptable. The optimized muffins were then analyzed for their textural, physicochemical and nutritional properties. The incorporation of CMP significantly affected the muffin’s texture. This Kodo millet-based, protein-enriched muffin offers a gluten-free alternative while maximizing the health benefits associated with Kodo millet.
Afficher plus [+] Moins [-]PERINEAL HERNIA IN A CROSSBRED JERSEY COW AND ITS SUCCESSFUL SURGICAL MANAGEMENT - A CASE REPORT Texte intégral
2025
P. Sankar | S. Jaianusiya | E. Kalaiselvan | S. Senthilkumar | E. Venkatesakumar | T. Sarath
A five-and-a-half-year-old Jersey cross bred cow was presented with the complaint of a protruding mass onright lateral aspect ofvulval lip for the past 10 days. Clinical examination revealed a soft, fluctuating, non-painful reducible mass measuring 8 inch in length and 6 inch in width on the right side of the perineal region. Based on ultrasonographic and radiographic evaluations, it was diagnosed as a case of perineal hernia and surgical intervention was advocated. Surgical correction was performed under xylazine-ketamine-butorphanol sedation and epidural anesthesia using 2% lignocaine and butorphanol. Herniorrhaphy was successfully completed using polyester No.5 suture. Skin closure was done using No.1 polyamide suture. Post-operative care included wound management, antibiotic therapy, and pain management. Sutures were removed on 15th day and animal was made an uneventful recovery.
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