Clinical indicators such as diarrhoea (DISCO) or anaemia (FAMACHA©) are used as a measure for targeted selective treatments against gastrointestinal nematodes (GIN). Enteric cestodes such as Moniezia may interfere directly with DISCO or indirectly with the FAMACHA© score. We investigated 821 Ouled Djellal rams naturally infected in a steppe environment (GIN alone, cestodes alone, GIN and cestodes) or not. The rams were treated with ivermectin 2 months before being slaughtered to reduce the impact of nematodes on the clinical scores; however, persistent or newly acquired GINs were not related to both scores. Of the non-infected rams (n = 296), 26% identified as needing treatment against GIN using the FAMACHA score, and 34.5% using DISCO would have been thus selected. This implies that the clinical indicators used for the targeted selective treatment of gastrointestinal nematodes are not fully reliable when a low infection is recorded and may well be influenced by confounding factors. As expected, only DISCO was affected by cestode infection, and we suggest that the presence of Moniezia should also be taken into consideration.

Clinical indicators such as diarrhoea (DISCO) or anaemia (FAMACHA©) are used as a measure for targeted selective treatments against gastrointestinal nematodes (GIN). Enteric cestodes such as Moniezia may interfere directly with DISCO or indirectly with the FAMACHA© score. We investigated 821 Ouled Djellal rams naturally infected in a steppe environment (GIN alone, cestodes alone, GIN and cestodes) or not. The rams were treated with ivermectin 2 months before being slaughtered to reduce the impact of nematodes on the clinical scores; however, persistent or newly acquired GINs were not related to both scores. Of the non-infected rams (n = 296), 26% identified as needing treatment against GIN using the FAMACHA score, and 34.5% using DISCO would have been thus selected. This implies that the clinical indicators used for the targeted selective treatment of gastrointestinal nematodes are not fully reliable when a low infection is recorded and may well be influenced by confounding factors. As expected, only DISCO was affected by cestode infection, and we suggest that the presence of Moniezia should also be taken into consideration.

International audience | Sheep enteric cestodes and their influence on clinical indicators used in targeted selective treatments against gastrointestinal nematodes',Clinical indicators such as diarrhoea (DISCO) or anaemia (FAMACHA©) are used as a measure for targeted selective treatments against gastrointestinal nematodes (GIN). Enteric cestodes such as Moniezia may interfere directly with DISCO or indirectly with the FAMACHA© score. We investigated 821 Ouled Djellal rams naturally infected in a steppe environment (GIN alone, cestodes alone, GIN and cestodes) or not. The rams were treated with ivermectin 2 months before being slaughtered to reduce the impact of nematodes on the clinical scores; however, persistent or newly acquired GINs were not related to both scores. Of the non-infected rams (n = 296), 26% identified as needing treatment against GIN using the FAMACHA score, and 34.5% using DISCO would have been thus selected. This implies that the clinical indicators used for the targeted selective treatment of gastrointestinal nematodes are not fully reliable when a low infection is recorded and may well be influenced by confounding factors. As expected, only DISCO was affected by cestode infection, and we suggest that the presence of Moniezia should also be taken into consideration. | Des indicateurs cliniques tels que la diarrhée (DISCO) ou l'anémie (FAMACHA © ) sont utilisés comme mesure pour des traitements sélectifs ciblés contre les nématodes gastro-intestinaux (GIN). Les cestodes entériques tels que Moniezia peuvent interférer directement avec DISCO ou indirectement avec le score FAMACHA © . Nous avons enquêté sur 821 béliers Ouled Djellal naturellement infectés en milieu steppique (GIN seul, cestodes seuls, GIN et cestodes) ou non. Les béliers ont été traités à l'ivermectine 2 mois avant d'être abattus pour réduire l'impact des nématodes sur les scores cliniques ; cependant, les GIN persistants ou nouvellement acquis n'étaient pas liés aux deux scores. Parmi les béliers non infectés ( n = 296), 26% identifiés comme ayant besoin d'un traitement contre le GIN selon le score FAMACHA, et 34,5% selon DISCO auraient ainsi été sélectionnés. Cela implique que les indicateurs cliniques utilisés pour le traitement sélectif ciblé des nématodes gastro-intestinaux ne sont pas entièrement fiables lorsqu'une faible infection est enregistrée et peuvent bien être influencés par des facteurs de confusion. Comme prévu, seul DISCO a été affecté par l'infection par cestode, et nous suggérons que la présence de Moniezia soit également prise en considération.

International audience | Clinical indicators such as diarrhoea (DISCO) or anaemia (FAMACHA©) are used as a measure for targeted selective treatments against gastrointestinal nematodes (GIN). Enteric cestodes such as Moniezia may interfere directly with DISCO or indirectly with the FAMACHA© score. We investigated 821 Ouled Djellal rams naturally infected in a steppe environment (GIN alone, cestodes alone, GIN and cestodes) or not. The rams were treated with ivermectin 2 months before being slaughtered to reduce the impact of nematodes on the clinical scores; however, persistent or newly acquired GINs were not related to both scores. Of the non-infected rams (n = 296), 26% identified as needing treatment against GIN using the FAMACHA score, and 34.5% using DISCO would have been thus selected. This implies that the clinical indicators used for the targeted selective treatment of gastrointestinal nematodes are not fully reliable when a low infection is recorded and may well be influenced by confounding factors. As expected, only DISCO was affected by cestode infection, and we suggest that the presence of Moniezia should also be taken into consideration. | Des indicateurs cliniques tels que la diarrhée (DISCO) ou l'anémie (FAMACHA © ) sont utilisés comme mesure pour des traitements sélectifs ciblés contre les nématodes gastro-intestinaux (GIN). Les cestodes entériques tels que Moniezia peuvent interférer directement avec DISCO ou indirectement avec le score FAMACHA © . Nous avons enquêté sur 821 béliers Ouled Djellal naturellement infectés en milieu steppique (GIN seul, cestodes seuls, GIN et cestodes) ou non. Les béliers ont été traités à l'ivermectine 2 mois avant d'être abattus pour réduire l'impact des nématodes sur les scores cliniques ; cependant, les GIN persistants ou nouvellement acquis n'étaient pas liés aux deux scores. Parmi les béliers non infectés ( n = 296), 26% identifiés comme ayant besoin d'un traitement contre le GIN selon le score FAMACHA, et 34,5% selon DISCO auraient ainsi été sélectionnés. Cela implique que les indicateurs cliniques utilisés pour le traitement sélectif ciblé des nématodes gastro-intestinaux ne sont pas entièrement fiables lorsqu'une faible infection est enregistrée et peuvent bien être influencés par des facteurs de confusion. Comme prévu, seul DISCO a été affecté par l'infection par cestode, et nous suggérons que la présence de Moniezia soit également prise en considération.

This epidemiological study aimed to determine the species of tick infestation in dogs, their prevalence and dynamic in the Bejaia province, northeastern Algeria. A total of 631 dogs were examined from different localities of the Bejaia province between March 2016 and February 2017. Of the 631 examined dogs, 15% were infested with one or more tick species. A total of 339 adult ticks were collected and identified, including 199 male tick species and 140 female tick species. Our results revealed that most of these were Rhipicephalus species, with Rhipicephalus sanguineus (51.32%) being the most prevalent followed by Rhipicephalus bursa (35.1%) and Rhipicephalus turanicus (12.98%). Ixodes ricinus represented only 0.6% of all ticks collected. The highest infested seasons were spring (22.55%) and summer (22.54%) and the lowest infested seasons were autumn (8.62%) and winter ( 0.9%). There is no significant difference between the sex of the animal and the prevalence of infestation (p = 0.837). Also, the prevalence of infestation by ticks in young animals was higher than that in adult animals (p = 0.550). A significant difference between the prevalence of infestation and animal breed was observed (p = 0.042). This study is the first epidemiological investigation conducted on the prevalence of hard ticks infesting domestic dogs in Bejaia (northeastern Algeria) based on conventional methods. It is therefore necessary to implement an effective tick control strategy during infestation periods in order to prevent vector-borne diseases.

This epidemiological study aimed to determine the species of tick infestation in dogs, their prevalence and dynamic in the Bejaia province, northeastern Algeria. A total of 631 dogs were examined from different localities of the Bejaia province between March 2016 and February 2017. Of the 631 examined dogs, 15% were infested with one or more tick species. A total of 339 adult ticks were collected and identified, including 199 male tick species and 140 female tick species. Our results revealed that most of these were Rhipicephalus species, with Rhipicephalus sanguineus (51.32%) being the most prevalent followed by Rhipicephalus bursa (35.1%) and Rhipicephalus turanicus (12.98%). Ixodes ricinus represented only 0.6% of all ticks collected. The highest infested seasons were spring (22.55%) and summer (22.54%) and the lowest infested seasons were autumn (8.62%) and winter ( 0.9%). There is no significant difference between the sex of the animal and the prevalence of infestation (p = 0.837). Also, the prevalence of infestation by ticks in young animals was higher than that in adult animals (p = 0.550). A significant difference between the prevalence of infestation and animal breed was observed (p = 0.042). This study is the first epidemiological investigation conducted on the prevalence of hard ticks infesting domestic dogs in Bejaia (northeastern Algeria) based on conventional methods. It is therefore necessary to implement an effective tick control strategy during infestation periods in order to prevent vector-borne diseases.

Shrubs represent the most affordable and accessible form of feed that livestock can rely on to acquire both essential and non-essential elements of life. In addition to their inherent toxins, they contain endogenous substances commonly referred to as ‘antinutritive factors’ (ANFs) that often interfere with the utilisation of nutrients. Their abundance may lead to severe clinical trauma. Hence, the objective of the study was to investigate the effects of different extraction techniques on Nerium oleander L. and animal feeds as well as to quantify oxalates. Organic (hexane, acetone and methanol) sequential and aqueous (infusion and decoction) extractions were explored. Qualitative and quantitative analyses were conducted to determine the presence of various phytochemicals and oxalate contents as putative ANFs, respectively. The results showed higher extraction yields of 22.6% and 43.1% in the decoction and infusion of N. oleander, respectively. The quantification methods were validated for linearity, accuracy and precision. Oxalate contents of 6.76 ± 0.245 (0.65%) mg/g and 5.74 ± 0.236 mg/g dry weight (0.55%) were obtained in N. oleander and feeds, respectively. This difference was statistically significant with p 0.05. Percentage recoveries of 98.5 (percent relative standard deviation [% RSD] = 2.3), 85.7 (% RSD = 1.03) and 80.3 (% RSD = 1.22) at 76%, 95% and 112% fortifications were obtained, respectively. Relative standard deviation for precision was 0.99% and 1.13% at 0.33 mg and 0.39 mg fortifications, respectively, while reproducibility showed 2.21% RSD. Therefore, these methods can be used to provide a valuable basis for qualitative determination of ANFs, particularly in shrub foliage.

Shrubs represent the most affordable and accessible form of feed that livestock can rely on to acquire both essential and non-essential elements of life. In addition to their inherent toxins, they contain endogenous substances commonly referred to as ‘antinutritive factors’ (ANFs) that often interfere with the utilisation of nutrients. Their abundance may lead to severe clinical trauma. Hence, the objective of the study was to investigate the effects of different extraction techniques on Nerium oleander L. and animal feeds as well as to quantify oxalates. Organic (hexane, acetone and methanol) sequential and aqueous (infusion and decoction) extractions were explored. Qualitative and quantitative analyses were conducted to determine the presence of various phytochemicals and oxalate contents as putative ANFs, respectively. The results showed higher extraction yields of 22.6% and 43.1% in the decoction and infusion of N. oleander, respectively. The quantification methods were validated for linearity, accuracy and precision. Oxalate contents of 6.76 ± 0.245 (0.65%) mg/g and 5.74 ± 0.236 mg/g dry weight (0.55%) were obtained in N. oleander and feeds, respectively. This difference was statistically significant with p < 0.05. Percentage recoveries of 98.5 (percent relative standard deviation [% RSD] = 2.3), 85.7 (% RSD = 1.03) and 80.3 (% RSD = 1.22) at 76%, 95% and 112% fortifications were obtained, respectively. Relative standard deviation for precision was 0.99% and 1.13% at 0.33 mg and 0.39 mg fortifications, respectively, while reproducibility showed 2.21% RSD. Therefore, these methods can be used to provide a valuable basis for qualitative determination of ANFs, particularly in shrub foliage.

South Africa is a large country of approximately 1.22 million km2, made up of nine provinces with three climatic zones. Farming in the country is mostly defined by regional differences. Of the different organisms isolated from milk samples of dairy cows, Staphylococcus aureus poses a challenge to maintain udder health and wholesome dairy products for human consumption. Antibiotic resistant bacteria are therefore a potential health hazard. The objective of this study was to investigate the seasonal and regional relationships of antibiotic resistance of S. aureus, of which little is known. This study was undertaken to evaluate a data set of 3410 S. aureus isolates, taken from milk samples with a somatic cell count of 400 000 cells/mL from commercial dairy herds. These isolates were tested for antimicrobial susceptibility using the Kirby Bauer method for ampicillin, cloxacillin, penicillin G, clindamycin, oxy-tetracycline, cephalexin, cefuroxime and tylosin. The samples were from 830 dairy herds, out of the estimated 2000 commercial dairy herds in South Africa. All the antibiotics tested, except for cephalosporins, showed a predicted prevalence of resistance of above 50% in most provinces, which is a concern. The lowest prevalence of resistance to the majority of the categories of antibiotics tested was present in KwaZulu-Natal during spring. The cephalosporins had the lowest levels of prevalence of bacterial resistance in Gauteng during winter. Resistance patterns of S. aureus to the eight antibiotics varied in the different seasons and provinces, possibly because of different weather conditions, and the action and spectrum of antibiotics.

South Africa is a large country of approximately 1.22 million km2, made up of nine provinces with three climatic zones. Farming in the country is mostly defined by regional differences. Of the different organisms isolated from milk samples of dairy cows, Staphylococcus aureus poses a challenge to maintain udder health and wholesome dairy products for human consumption. Antibiotic resistant bacteria are therefore a potential health hazard. The objective of this study was to investigate the seasonal and regional relationships of antibiotic resistance of S. aureus, of which little is known. This study was undertaken to evaluate a data set of 3410 S. aureus isolates, taken from milk samples with a somatic cell count of > 400 000 cells/mL from commercial dairy herds. These isolates were tested for antimicrobial susceptibility using the Kirby Bauer method for ampicillin, cloxacillin, penicillin G, clindamycin, oxy-tetracycline, cephalexin, cefuroxime and tylosin. The samples were from 830 dairy herds, out of the estimated 2000 commercial dairy herds in South Africa. All the antibiotics tested, except for cephalosporins, showed a predicted prevalence of resistance of above 50% in most provinces, which is a concern. The lowest prevalence of resistance to the majority of the categories of antibiotics tested was present in KwaZulu-Natal during spring. The cephalosporins had the lowest levels of prevalence of bacterial resistance in Gauteng during winter. Resistance patterns of S. aureus to the eight antibiotics varied in the different seasons and provinces, possibly because of different weather conditions, and the action and spectrum of antibiotics.

International audience | South Africa is a large country of approximately 1.22 million km(2), made up of nine provinces with three climatic zones. Farming in the country is mostly defined by regional differences. Of the different organisms isolated from milk samples of dairy cows, Staphylococcus aureus poses a challenge to maintain udder health and wholesome dairy products for human consumption. Antibiotic resistant bacteria are therefore a potential health hazard. The objective of this study was to investigate the seasonal and regional relationships of antibiotic resistance of S. aureus, of which little is known. This study was undertaken to evaluate a data set of 3410 S. aureus isolates, taken from milk samples with a somatic cell count of > 400 000 cells/mL from commercial dairy herds. These isolates were tested for antimicrobial susceptibility using the Kirby Bauer method for ampicillin, cloxacillin, penicillin G, clindamycin, oxy-tetracycline, cephalexin, cefuroxime and tylosin. The samples were from 830 dairy herds, out of the estimated 2000 commercial dairy herds in South Africa. All the antibiotics tested, except for cephalosporins, showed a predicted prevalence of resistance of above 50% in most provinces, which is a concern. The lowest prevalence of resistance to the majority of the categories of antibiotics tested was present in KwaZulu-Natal during spring. The cephalosporins had the lowest levels of prevalence of bacterial resistance in Gauteng during winter. Resistance patterns of S. aureus to the eight antibiotics varied in the different seasons and provinces, possibly because of different weather conditions, and the action and spectrum of antibiotics.

Canine babesiosis is a virulent infection of dogs in South Africa caused principally by Babesia rossi. Hypovitaminosis D has been reported in a wide range of infectious diseases in humans and dogs, and low vitamin D status has been associated with poor clinical outcomes. However, the relationship between vitamin D status and canine babesiosis has not been investigated. The objective of this study was to examine the relationship between the presence and severity of B. rossi infection and vitamin D status of infected dogs. Owners with dogs with a confirmed diagnosis of B. rossi infection and of healthy control dogs were invited to enrol onto the study. Vitamin D status was assessed by measurement of serum concentrations of the major circulating vitamin D metabolite, 25-hydroxyvitamin D (25[OH]D). Dogs with babesiosis (n = 34) had significantly lower mean serum 25(OH)D concentrations than healthy dogs (n = 24) (37.76 ± 21.25 vs. 74.2 ± 20.28 nmol/L). The effect of babesiosis on serum 25(OH)D concentrations was still significant after adjusting for any effect of age, body weight and sex. There was a negative relationship between serum 25(OH)D concentrations and disease severity in dogs with babesiosis. Serum concentrations of creatinine and alanine aminotransferase and time to last meal were not associated with serum 25(OH)D concentrations in dogs with babesiosis. In conclusion, dogs with Babesia rossi infections had lower serum 25(OH)D concentrations than healthy dogs. The inverse correlation between 25(OH)D concentrations and the clinical severity score indicate that hypovitaminosis D might be a helpful additional indicator of disease severity.

Canine babesiosis is a virulent infection of dogs in South Africa caused principally by Babesia rossi. Hypovitaminosis D has been reported in a wide range of infectious diseases in humans and dogs, and low vitamin D status has been associated with poor clinical outcomes. However, the relationship between vitamin D status and canine babesiosis has not been investigated. The objective of this study was to examine the relationship between the presence and severity of B. rossi infection and vitamin D status of infected dogs. Owners with dogs with a confirmed diagnosis of B. rossi infection and of healthy control dogs were invited to enrol onto the study. Vitamin D status was assessed by measurement of serum concentrations of the major circulating vitamin D metabolite, 25-hydroxyvitamin D (25[OH]D). Dogs with babesiosis (n = 34) had significantly lower mean serum 25(OH)D concentrations than healthy dogs (n = 24) (37.76 ± 21.25 vs. 74.2 ± 20.28 nmol/L). The effect of babesiosis on serum 25(OH)D concentrations was still significant after adjusting for any effect of age, body weight and sex. There was a negative relationship between serum 25(OH)D concentrations and disease severity in dogs with babesiosis. Serum concentrations of creatinine and alanine aminotransferase and time to last meal were not associated with serum 25(OH)D concentrations in dogs with babesiosis. In conclusion, dogs with Babesia rossi infections had lower serum 25(OH)D concentrations than healthy dogs. The inverse correlation between 25(OH)D concentrations and the clinical severity score indicate that hypovitaminosis D might be a helpful additional indicator of disease severity.

Infectious diseases are serious constraints for improving livestock productivity. Bovine viral diarrhoea virus (BVDV) is a virus causing grave economic losses throughout the cattle producing world. Infection is often not apparent, but the virus can also cause respiratory signs, diarrhoea, reproductive problems and immunosuppression. Risk factors for disease transmission include, but are not limited to, herd size, animal trade and grazing on communal pastures. Several prevalence studies have been conducted in southern Africa, but in Botswana the occurrence is largely unknown. In this study, blood samples were obtained from 100 goats from three villages around the capital city, Gaborone. Also, 364 blood samples from cattle around Gaborone, collected as part of another study, were analysed. The detected antibody prevalence was 0% in goats and 53.6% in cattle when using a competitive enzyme-linked immunoassay. Three animals from two different herds were positive for viral nucleic acids on polymerase chain reaction. The two herds with viraemic animals had significantly higher antibody prevalence compared to the other herds. Also, two of the detected viruses were sequenced and found to be most similar to BVDV-1a. To the authors’ knowledge, this is the first time that sequencing has been performed on BVDV isolated in Botswana.

Infectious diseases are serious constraints for improving livestock productivity. Bovine viral diarrhoea virus (BVDV) is a virus causing grave economic losses throughout the cattle producing world. Infection is often not apparent, but the virus can also cause respiratory signs, diarrhoea, reproductive problems and immunosuppression. Risk factors for disease transmission include, but are not limited to, herd size, animal trade and grazing on communal pastures. Several prevalence studies have been conducted in southern Africa, but in Botswana the occurrence is largely unknown. In this study, blood samples were obtained from 100 goats from three villages around the capital city, Gaborone. Also, 364 blood samples from cattle around Gaborone, collected as part of another study, were analysed. The detected antibody prevalence was 0% in goats and 53.6% in cattle when using a competitive enzyme-linked immunoassay. Three animals from two different herds were positive for viral nucleic acids on polymerase chain reaction. The two herds with viraemic animals had significantly higher antibody prevalence compared to the other herds. Also, two of the detected viruses were sequenced and found to be most similar to BVDV-1a. To the authors’ knowledge, this is the first time that sequencing has been performed on BVDV isolated in Botswana.

Several types of odours are involved in the location of host animals by tsetse (Diptera: Glossinidae), a vector of animal African trypanosomiasis. Host animals’ ageing urine has been shown to be the source of a phenolic blend attractive to the tsetse. Nevertheless, limited research has been performed on the microbial communities’ role in the production of phenols. This study aimed at profiling bacterial communities mediating the production of tsetse attractive phenols in mammalian urine. Urine samples were collected from African buffalo (Syncerus caffer), cattle (Bos taurus) and eland (Taurotragus oryx) at Kongoni Game Valley Ranch and Kenyatta University in Kenya. Urine samples, of each animal species, were pooled and left open to age in ambient conditions. Bacteriological and phenols analyses were then carried out, at 4 days ageing intervals, for 24 days. Phenols analysis revealed nine volatile phenols: 4-cresol, ortho-cresol, 3-cresol, phenol, 3-ethylphenol, 3-propylphenol, 2-methyloxyphenol, 4-ethylphenol and 4-propylphenol. Eight out of 19 bacterial isolates from the ageing urine revealed the potential to mediate production of phenols. 16S rRNA gene characterisation of the isolates closely resembled Enterococcus faecalis KUB3006, Psychrobacter alimentarius PAMC 27887, Streptococcus agalactiae 2603V, Morganella morganii sub.sp. morganii KT, Micrococcus luteus NCTC2665, Planococcus massiliensis strain ES2, Ochrobactrum pituitosum AA2 and Enterococcus faecalis OGIRF. This study established that some of the phenols emitted from mammalian urine, which influence the tsetse‘s host-seeking behaviour, are well characterised by certain bacteria. These results may allow the development of biotechnological models in vector control that combines the use of these bacteria in the controlled release of semiochemicals.

Several types of odours are involved in the location of host animals by tsetse (Diptera: Glossinidae), a vector of animal African trypanosomiasis. Host animals’ ageing urine has been shown to be the source of a phenolic blend attractive to the tsetse. Nevertheless, limited research has been performed on the microbial communities’ role in the production of phenols. This study aimed at profiling bacterial communities mediating the production of tsetse attractive phenols in mammalian urine. Urine samples were collected from African buffalo (Syncerus caffer), cattle (Bos taurus) and eland (Taurotragus oryx) at Kongoni Game Valley Ranch and Kenyatta University in Kenya. Urine samples, of each animal species, were pooled and left open to age in ambient conditions. Bacteriological and phenols analyses were then carried out, at 4 days ageing intervals, for 24 days. Phenols analysis revealed nine volatile phenols: 4-cresol, ortho-cresol, 3-cresol, phenol, 3-ethylphenol, 3-propylphenol, 2-methyloxyphenol, 4-ethylphenol and 4-propylphenol. Eight out of 19 bacterial isolates from the ageing urine revealed the potential to mediate production of phenols. 16S rRNA gene characterisation of the isolates closely resembled Enterococcus faecalis KUB3006, Psychrobacter alimentarius PAMC 27887, Streptococcus agalactiae 2603V, Morganella morganii sub.sp. morganii KT, Micrococcus luteus NCTC2665, Planococcus massiliensis strain ES2, Ochrobactrum pituitosum AA2 and Enterococcus faecalis OGIRF. This study established that some of the phenols emitted from mammalian urine, which influence the tsetse‘s host-seeking behaviour, are well characterised by certain bacteria. These results may allow the development of biotechnological models in vector control that combines the use of these bacteria in the controlled release of semiochemicals.

Toxoplasma gondii is a major neglected parasitic infection occurring in settings of extreme poverty in Africa. Apart from causing reproductive failure in animals it is also a significant zoonotic concern. The objective of this study was to determine the seroprevalence and associated risk factors of T. gondii infection in cats, chickens, goats, sheep and pigs in the southeast of South Africa, of which little is known. Sera was obtained from 601 domestic animals including 109 cats, 137 chickens, 128 goats, 121 sheep and 106 pigs managed under different production systems in different agro-ecological regions and evaluated by the Toxoreagent, a latex agglutination test for T. gondii antibody detection. Household-level and animal-level data were collected by interviewing animal owners and/or herders using a closed-ended questionnaire. The study revealed an overall farm seroprevalence of 83.33% (125/150 farms) with the highest rate of infection for the parasite found in sheep with 64.46% (78/121), followed by goats with 53.91% (69/128), pigs with 33.96% (36/106), cats with 32.11% (35/109 cats) and chickens with 33.58% (46/137). The risk factors that were found to be statistically significant (p 0.05) to different species of seropositivites were age, location, climate, animal production system, rodent control, seropositive cat, cat-feed access and cat faecal disposal. The relatively high seroprevalence of T. gondii detected in this region suggests that domestic animals may pose a substantial public health risk through the consumption of T. gondii-infected raw meat as well as via contact with cat faeces.

International audience | Toxoplasma gondii is a major neglected parasitic infection occurring in settings of extreme poverty in Africa. Apart from causing reproductive failure in animals it is also a significant zoonotic concern. The objective of this study was to determine the seroprevalence and associated risk factors of T. gondii infection in cats, chickens, goats, sheep and pigs in the southeast of South Africa, of which little is known. Sera was obtained from 601 domestic animals including 109 cats, 137 chickens, 128 goats, 121 sheep and 106 pigs managed under different production systems in different agro-ecological regions and evaluated by the Toxoreagent, a latex agglutination test for T. gondii antibody detection. Household-level and animal-level data were collected by interviewing animal owners and/or herders using a closed-ended questionnaire. The study revealed an overall farm seroprevalence of 83.33% (125/150 farms) with the highest rate of infection for the parasite found in sheep with 64.46% (78/121), followed by goats with 53.91% (69/128), pigs with 33.96% (36/106), cats with 32.11% (35/109 cats) and chickens with 33.58% (46/137). The risk factors that were found to be statistically significant (p < 0.05) to different species of seropositivites were age, location, climate, animal production system, rodent control, seropositive cat, cat-feed access and cat faecal disposal. The relatively high seroprevalence of T. gondii detected in this region suggests that domestic animals may pose a substantial public health risk through the consumption of T. gondii-infected raw meat as well as via contact with cat faeces.

Toxoplasma gondii is a major neglected parasitic infection occurring in settings of extreme poverty in Africa. Apart from causing reproductive failure in animals it is also a significant zoonotic concern. The objective of this study was to determine the seroprevalence and associated risk factors of T. gondii infection in cats, chickens, goats, sheep and pigs in the southeast of South Africa, of which little is known. Sera was obtained from 601 domestic animals including 109 cats, 137 chickens, 128 goats, 121 sheep and 106 pigs managed under different production systems in different agro-ecological regions and evaluated by the Toxoreagent, a latex agglutination test for T. gondii antibody detection. Household-level and animal-level data were collected by interviewing animal owners and/or herders using a closed-ended questionnaire. The study revealed an overall farm seroprevalence of 83.33% (125/150 farms) with the highest rate of infection for the parasite found in sheep with 64.46% (78/121), followed by goats with 53.91% (69/128), pigs with 33.96% (36/106), cats with 32.11% (35/109 cats) and chickens with 33.58% (46/137). The risk factors that were found to be statistically significant (p < 0.05) to different species of seropositivites were age, location, climate, animal production system, rodent control, seropositive cat, cat-feed access and cat faecal disposal. The relatively high seroprevalence of T. gondii detected in this region suggests that domestic animals may pose a substantial public health risk through the consumption of T. gondii-infected raw meat as well as via contact with cat faeces.

Toxoplasma gondii is a major neglected parasitic infection occurring in settings of extreme poverty in Africa. Apart from causing reproductive failure in animals it is also a significant zoonotic concern. The objective of this study was to determine the seroprevalence and associated risk factors of T. gondii infection in cats, chickens, goats, sheep and pigs in the southeast of South Africa, of which little is known. Sera was obtained from 601 domestic animals including 109 cats, 137 chickens, 128 goats, 121 sheep and 106 pigs managed under different production systems in different agro-ecological regions and evaluated by the Toxoreagent, a latex agglutination test for T. gondii antibody detection. Household-level and animal-level data were collected by interviewing animal owners and/or herders using a closed-ended questionnaire. The study revealed an overall farm seroprevalence of 83.33% (125/150 farms) with the highest rate of infection for the parasite found in sheep with 64.46% (78/121), followed by goats with 53.91% (69/128), pigs with 33.96% (36/106), cats with 32.11% (35/109 cats) and chickens with 33.58% (46/137). The risk factors that were found to be statistically significant (p 0.05) to different species of seropositivites were age, location, climate, animal production system, rodent control, seropositive cat, cat-feed access and cat faecal disposal. The relatively high seroprevalence of T. gondii detected in this region suggests that domestic animals may pose a substantial public health risk through the consumption of T. gondii-infected raw meat as well as via contact with cat faeces.

Salmonellosis is a major threat facing the poultry industry globally. This study was conducted to investigate the level of Salmonella contaminations and determine the resistance pattern of isolates obtained from selected poultry farms in Kwara State, a transition state between southern and northern regions of Nigeria. A total of 900 samples were collected between January and August 2017, from the poultry environment, apparently including healthy and dead birds. Salmonella was isolated and identified using standard bacteriological methods. All presumptive Salmonella isolates were serotyped and tested for antimicrobial susceptibility using 11 different antimicrobials. A total of 58 (6.4%) Salmonella isolates were obtained, and the isolation rate was only statistically significant (p 0.05) in live birds. The isolates comprised of 13 serovars. The three predominant serovars, Salmonella enterica ser. 6.7:d:- (29.0%), Salmonella Agama (28.0%) and Salmonella Typhimurium (16.0%), were isolated from all three sample types. Rare serovars like Salmonella Albany, Salmonella Colindale, Salmonella Istanbul, Salmonella Larochelle, Salmonella Nigeria and Salmonella Orion were also isolated in this study. A high frequency of resistance was generally observed with all the isolates exhibiting a total of (100%) resistance to ampicillin, cefotaxime and ceftazidime. This study documents the first predominant isolation of S. enterica ser. 6.7:d:- and S. Agama from chickens. It also documents the high frequency of fluoroquinolone and cephalosporins resistance of the isolates indicating the presence of selective pressure in the environment. Controls and targeted interventions against Salmonella and the frequent occurrence of antimicrobial resistance in chickens should be initiated to prevent the spread of this organism.

Salmonellosis is a major threat facing the poultry industry globally. This study was conducted to investigate the level of Salmonella contaminations and determine the resistance pattern of isolates obtained from selected poultry farms in Kwara State, a transition state between southern and northern regions of Nigeria. A total of 900 samples were collected between January and August 2017, from the poultry environment, apparently including healthy and dead birds. Salmonella was isolated and identified using standard bacteriological methods. All presumptive Salmonella isolates were serotyped and tested for antimicrobial susceptibility using 11 different antimicrobials. A total of 58 (6.4%) Salmonella isolates were obtained, and the isolation rate was only statistically significant (p < 0.05) in live birds. The isolates comprised of 13 serovars. The three predominant serovars, Salmonella enterica ser. 6.7:d:- (29.0%), Salmonella Agama (28.0%) and Salmonella Typhimurium (16.0%), were isolated from all three sample types. Rare serovars like Salmonella Albany, Salmonella Colindale, Salmonella Istanbul, Salmonella Larochelle, Salmonella Nigeria and Salmonella Orion were also isolated in this study. A high frequency of resistance was generally observed with all the isolates exhibiting a total of (100%) resistance to ampicillin, cefotaxime and ceftazidime. This study documents the first predominant isolation of S. enterica ser. 6.7:d:- and S. Agama from chickens. It also documents the high frequency of fluoroquinolone and cephalosporins resistance of the isolates indicating the presence of selective pressure in the environment. Controls and targeted interventions against Salmonella and the frequent occurrence of antimicrobial resistance in chickens should be initiated to prevent the spread of this organism.

Rift Valley fever (RVF) is a zoonotic viral disease caused by the RVF phlebovirus (RVFV) that infects a variety of animal species including sheep and goats. Sera (n = 893) collected between 2013 and 2015 from randomly selected indigenous sheep and goats in seven provinces of the Democratic Republic of the Congo (DRC) were tested for the presence of specific immunoglobulin G (IgG) and M (IgM) against RVFV, using two commercially available enzyme-linked immunosorbent assays. The reverse transcription polymerase chain reaction (RT-PCR) was also used to detect RVFV nucleic acid. There was significant variation in true seroprevalence of RVFV for both sheep and goats between the seven provinces investigated. Values ranged from 0.0 (95% confidence interval [CI] 0.0–6.55) to 23.81 (95% CI 12.03–41.76) for goat and 0.0 (95% CI 0.0–7.56) to 37.11 (95% CI 15.48–65.94) for sheep, respectively. One serum (1.85%) out of 54 that tested positive for IgG was found to be IgM-positive. This same sample was also positive by RT-PCR indicating an active or recent infection. These findings report the presence of RVFV in small ruminants in the DRC for the first time and indicate variations in exposure to the virus in different parts of the country.

Rift Valley fever (RVF) is a zoonotic viral disease caused by the RVF phlebovirus (RVFV) that infects a variety of animal species including sheep and goats. Sera (n = 893) collected between 2013 and 2015 from randomly selected indigenous sheep and goats in seven provinces of the Democratic Republic of the Congo (DRC) were tested for the presence of specific immunoglobulin G (IgG) and M (IgM) against RVFV, using two commercially available enzyme-linked immunosorbent assays. The reverse transcription polymerase chain reaction (RT-PCR) was also used to detect RVFV nucleic acid. There was significant variation in true seroprevalence of RVFV for both sheep and goats between the seven provinces investigated. Values ranged from 0.0 (95% confidence interval [CI] 0.0–6.55) to 23.81 (95% CI 12.03–41.76) for goat and 0.0 (95% CI 0.0–7.56) to 37.11 (95% CI 15.48–65.94) for sheep, respectively. One serum (1.85%) out of 54 that tested positive for IgG was found to be IgM-positive. This same sample was also positive by RT-PCR indicating an active or recent infection. These findings report the presence of RVFV in small ruminants in the DRC for the first time and indicate variations in exposure to the virus in different parts of the country.