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Effect of water extract of Danshen on bone regeneration of rat calvarial defect model
2010
Shim, K.M., Nambu University, Gwangju, Republic of Korea | Kim, S.E., Chonnam National University, Gwangju, Republic of Korea | Kang, S.S., Chonnam National University, Gwangju, Republic of Korea
The purpose of this study was to evaluate the osteogenic capacity of water extract of danshen (Salvia miltiorrhiza Bunge). We have established in rat critical-sized calvarial defect model using the combination with collagen scaffold and danshen hydrophilic extract. All rats were extinguished at 8 weeks after bone graft surgery, and the bone regeneration ability of bone grafting sides was evaluated by plain radiography and micro-CT. These results revealed water extract of danshen had the potential to promote osteogenesis especially continuous oral administration with local treatment compared to one-shot local treatment. This compound may provide a new alternative agent for growth factors to promote bone healing and bone regeneration. In conclusion, these results suggest that danshen hydrophilic extract have the potential to promote osteogenesis in bone defects. Further studies about fusion technology with salvianolic acid B, peptides, growth factors, and scaffolds using of the combination of tissue engineering, cell engineering and mechanical engineering are needed.
Afficher plus [+] Moins [-]Biological activities and single oral dose toxicity in rat of fermented Rhus verniciflua extract
2010
Choi, M.J., Kyungpook National University, Daegu, Republic of Korea | Lee, S.J., Kyungpook National University, Daegu, Republic of Korea | Jang, S.H., Kyungpook National University, Daegu, Republic of Korea | Reza, Md. Ahsanur, Kyungpook National University, Daegu, Republic of Korea | Hong, J.H., Catholic University of Daegu, Gyungsan, Republic of Korea | Jung, H.K., Bio Industry Center, Daegu Technopark, Daegu, Republic of Korea | Park, S.C., Kyungpook National University, Daegu, Republic of Korea
In this study, we investigated the biological activities such as anti-tumor, anti-oxidant and antiinflammatory activities as well as single oral dose toxicity of fermented Rhus verniciflua extract (FRVE). In order to examine anti-tumor activity of FRVE, the sarcoma 180 cells were treated with FRVE at various concentrations (0.03, 0.3, 3 and 30 mg/mL) in microtetrazolium (MTT) assay. In MTT assay, all the cells treated with FRVE at various concentrations have shown a significant difference compared with control (p less than 0.05). In xanthine oxidase inhibition assay to examine the antioxidant activity, the xanthine oxidase inhibition rate of FRVE at 1.5 mg/mL and 15 mg/mL was 85±15.01% and 99±16.02%, respectively. Nitric oxide production in RAW 264.7 cells showed that FRVE showed a significant anti-inflammation effect at 3 mg/mL (p less than 0.05). In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs, body weight gains, feed and water consumptions. The results indicated that lethal dose 50 (LD∧50) of FRVE was found to be higher than 5,000 mg/kg in this experiment. From the above results, we may suggest that FRVE might have useful as a material for functional food and/ or animal pharmaceutics.
Afficher plus [+] Moins [-]Molecular epidemiologic analysis of pathogenic Escherichia coli isolated from poultry in Korea
2010
Sung, M.S., Kyungpook National University, Daegu, Republic of Korea | Kim, J.H., Kyungpook National University, Daegu, Republic of Korea | Kim, K.S., Kyungpook National University, Daegu, Republic of Korea
Among 203 avian pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis in korea, 14 isolates were selected from total 68 isolates transferred R plasmid and classified into 5 groups on the basis of antimicrobial minimal inhibitory concentration (MIC) pattern, farm source and O serotype. An association between clonal origin and R plasmid of them was investigated by R plasmid profile, restriction endonuclease analysis and pulsed-field gel electrophoresis (PFGE). The strains that showed the same or very similar antimicrobial MIC pattern, but different farm source and O serotype, revealed different PFGE pattern, which seemed to be different clonal origin. And the strains that showed the same MIC pattern and O serotype, revealed different PFGE pattern, seemed to be originated from different clone. Also the strains showing the same MIC pattern and farm source, but different O serotype, revealed to be different clonal origin. The strains that showed the same or similar MIC pattern, farm source, and O serotype, revealed identical or similar PFGE pattern, which seemed to belong to be one clone. Meanwhile, horizontal transfer of R plasmid seems to be common in APEC with regardless of O serotype and clone of the strains. These results indicate that rapid and accurate epidemiological survey of APEC can be possible by the combination of O serotyping, plasmid profiling and PFGE analysis following the classification of them into groups of antimicrobial drug resistance pattern.
Afficher plus [+] Moins [-]Investigation of helminths and protozoans infecting old world monkeys: captive vervet, cynomolgus, and rhesus monkeys
2010
Lee, J.I., Seoul National University Hospital, Seoul, Republic of Korea | Kang, S.J., Seoul National University Hospital, Seoul, Republic of Korea | Kim, N.A., Seoul National University Hospital, Seoul, Republic of Korea | Lee, C.W., Seoul National University Hospital, Seoul, Republic of Korea | Ahn, K.H., Seoul National University Hospital, Seoul, Republic of Korea | Kwon, H.S., Seoul National University Hospital, Seoul, Republic of Korea | Park, C.G., Seoul National University Hospital, Seoul, Republic of Korea | Kim, S.J., Seoul National University Hospital, Seoul, Republic of Korea
The objective of this study was to investigate the infection rate of gastro-intestinal tract parasites on acquired laboratory nonhuman primates, Vervet monkey, Cynomolgus monkey, and Rhesus monkey acquired from Japan and China. These monkeys have been acclimating at an individual housing condition after our legal quarantine period. We examined 133 fecal samples to investigate parasitic infection using direct smear and formalin-ether-sedimentation technique. As a result, total parasitic infection rate was 33.8% (n=45/133) for all monkeys. Two species of macaques, cynomolgus and rhesus, were infected with Trichuris trichiura (4), Giardia lamblia (4) and Balantidium coli (41). Vervet monkeys, which had been controlled by individual housing system for a long time, were clear for parasitic infection. The protozoan, Balantidium coli was one of the most frequently detected in these monkey colonies. Double infection was noted in only 4 monkeys and involved with Trichuris trichiura and Balantidium coli. Serious clinical symptoms were not observed in the most of the infected monkeys, but the monkeys infected by Giardia lamblia showed intermittent or chronic watery diarrhea. Consequently, the prophylactic anthelmintic treatment and periodic monitoring are essential to preserve the SPF colonies in the laboratory facility.
Afficher plus [+] Moins [-]Proteome analysis between diverse phenotypes of Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium)
2010
Shin, G.W., Chonbuk National University, Jeonju, Republic of Korea | Cha, I.S., Gyeongsang National University, Jinju, Republic of Korea | Lee, W.W., Busan Metropolitan City Institute of Health and Environmental, Busan, Republic of Korea | Nho, S.W., Gyeongsang National University, Jinju, Republic of Korea | Park, S.B., Gyeongsang National University, Jinju, Republic of Korea | Jang, H.B., Gyeongsang National University, Jinju, Republic of Korea | Kim, Y.H., Gyeongsang National University, Jinju, Republic of Korea | Jung, T.S., Gyeongsang National University, Jinju, Republic of Korea
Protein expression patterns in Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strains with diverse phenotypes, such as phage type, antibiotic resistance pattern and plasmid profiles were examined. For detailed analysis of proteins expressed by different S. Typhimurium strains, protein fractions were divided into detergent-rich phase (DP) and aqueous phase (AP) using triton X-114 detergent. The two phases were subjected to two-dimensional gel electrophoresis (2-DE), followed by protein identification using peptide mass fingerprinting (PMF). In the results, PMF showed that DP fractions consisted mainly of outer membrane proteins, whereas the AP fractions included cytosolic proteins. Comparison of 2-DE profiles of DP did not show any distinct protein spots which could be correlated with phage type, antibiotic resistance pattern or plasmid profile. However, comparisons of 2-DE profiles of the AP revealed differences in the protein spots, which could be correlated with the plasmid profile and phage types. Among these protein spots, flagellin was specific for strains containing a 90 kb plasmid. Compared to DT193 phage type, three protein spots in the range of pI 5.0-5.5 and MW 8-15 kDa of AP 2-DE profiles were absent in the DT104 phage types. Additionally, a protein spot with PI in the range of 4.5-5.0 and molecular weight (MW) between 51-69 kDa was specific for phage type DT104, while a protein spot with pI in the range of 4.0-4.8 and MW between 18-20 kDa was specific for DT193 phage type. These protein spots may be useful for discriminating phage types of S. Typhimurium.
Afficher plus [+] Moins [-]Clinical characteristics of oral diseases on stray cats in Gwangju
2010
Myung, B.Y., Gwangju Animal Shelter, Gwangju, Republic of Korea | Kim, S.E., Chonnam National University, Gwangju, Republic of Korea | Shim, K.M., Nambu University, Gwangju, Republic of Korea | Ryu, J.W., Chosun University, Gwangju, Republic of Korea | Kim, S.H., Chonnam National University, Gwangju, Republic of Korea | Moon, C.J., Chonnam National University, Gwangju, Republic of Korea | Bae, C.S., Chonnam National University, Gwangju, Republic of Korea | Choi, S.H., Chungbuk National University, Cheongju, Republic of Korea | Kang, S.S., Chonnam National University, Gwangju, Republic of Korea | Park, H.J., Jeju National University, Jeju, Republic of Korea
Demand on specialized feline dentistry has increased over the years, partly due to growing feline population in Korea. Despite the notable increase in the population, not much researches has been conducted on oral disease for stray cats. Thus this research was conducted on 81 adult stray cats from Gwangju in Korea (42 males and 39 females) with average weight of 3.4 kg and also focused on occurrence of periodontal disease (plaque and calculus), feline odontoclastic resorptive lesion (FORL), stomatitis and tooth anomaly. Formation of dental plaque and calculus were abundant on maxilla than mandible, especially distributed much on 4th premolar teeth and 1st molar teeth. After thorough examination of oral cavity, 33 stray cats (40.7%) were found to have oral disease. Eighteen stray cats (22.2%) had tooth fracture which was evident on cuspid and missing teeth were present on 2nd premolar tooth of maxilla and 2nd incisor of mandible amongst 20 stray cats (24.7%). FORL was present on cuspids and 2nd premolar tooth of maxilla among 10 stray cats (12.3%). Stomatitis was involved with FORL, missing tooth, and tooth fracture. Therefore, it was determined that stomatitis, FORL, and periodontal disease were closely associated.
Afficher plus [+] Moins [-]Cystorrhexis with uroperitoneum during parturition in a Appaloosa neonatal foal
2010
Yang, J.H., Equine Hospital of Busan Race Park, Korea Racing Authority, Busan, Republic of Korea | Cheong, J.T., Jeju National University, Jeju, Republic of Korea | Lee, J.M., Jeju National University, Jeju, Republic of Korea | Hwang, K.K., Jeju National University, Jeju, Republic of Korea | Lim, Y.K., Jeju National University, Jeju, Republic of Korea
A 3-day-old Appaloosa colt was examined because of acute onset of ataxia, left-sided head tilt, fever and heart murmur. The foal continued to nurse but became increasingly depressed and mild colic. On plain radiological examination, the foal was normal. Uroperitoneum was diagnosed following abdominocentesis. Postmortem examination revealed bladder rupture. The lesion was in the dorsomedial part of the bladder wall. We suggested that the cause of bladder rupture was parturient trauma. Even though the rupture was associated with trauma, the margin of rupture of bladder was clearly smooth and regular.
Afficher plus [+] Moins [-]Treatment of prednisolone for eosinophilic enteritis in a dog
2010
Lee, J.W., Chungnam National University, Daejeon, Republic of Korea | Lee, S.H., Chungnam National University, Daejeon, Republic of Korea | Lim, S.J., Chungnam National University, Daejeon, Republic of Korea | Park, H.J., Chungnam National University, Daejeon, Republic of Korea | Jung, D.W., Chungnam National University, Daejeon, Republic of Korea | Kim, T.S., Chungnam National University, Daejeon, Republic of Korea | Kim, D.H., Chungnam National University, Daejeon, Republic of Korea | Song, K.H., Chungnam National University, Daejeon, Republic of Korea
A 7-year-old, castrated male Yorkshire terrier dog with chronic vomiting, and melena lasted for 3 weeks was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. The complete blood counts and serum biochemistry revealed no remarkable findings. Gastrointestinal series revealed irregular margin in small intestinal lumen. After upper gastrointestinal series, erythema of the small intestinal lesions was found by exploratory laparotomy and full-thickness biopsy was performed. Histopathologic examination revealed infiltration of predominantly eosinophils and other inflammatory cells in small intestine. Based on these findings, the dog was diagnosed as eosinophilic enteritis. After 4 weeks medical treatment and hypoallergenic diet, the patient showed no vomiting, melena and other complications.
Afficher plus [+] Moins [-]Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium
2010
Choresca Jr. Casiano H., Seoul National University, Seoul, Republic of Korea | Gomez, Dennis K., University of the Philippines in the Visayas, Miagao, Iloilo, Republic of the Philippines | Han, J.E., Seoul National University, Seoul, Republic of Korea | Shin, S.P., Seoul National University, Seoul, Republic of Korea | Kim, J.H., Seoul National University, Seoul, Republic of Korea | Jun, J.W., Seoul National University, Seoul, Republic of Korea | Park, S.C., Seoul National University, Seoul, Republic of Korea
Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.
Afficher plus [+] Moins [-]Expression of vascular endothelial growth factor and endothelial nitric oxide synthase is increased in the placenta of sheep at high altitude in the Andes Texte intégral
2010
Parraguez, Victor H. | Atlagich, Miljenko A. | Urguieta, Bessie | Galleguillos, Marco | Reyes, Monica De los | Kooyman, David L. | Araneda, Silvia | Raggi, Luis A.
Expression of vascular endothelial growth factor and endothelial nitric oxide synthase is increased in the placenta of sheep at high altitude in the Andes Texte intégral
2010
Parraguez, Victor H. | Atlagich, Miljenko A. | Urguieta, Bessie | Galleguillos, Marco | Reyes, Monica De los | Kooyman, David L. | Araneda, Silvia | Raggi, Luis A.
Fetal weight and the placenta of sheep at high altitude (HA) are affected by hypoxia. Placental changes (an increase in placental size and vascularization) are greater in ewes from populations that have lived for several generations at HA than in those exposed during just 1 gestation. This study investigated placental expression of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS), 2 molecules involved in placental angiogenesis that could be upregulated by hypoxia. Two groups of ewes were maintained at HA (3589 m) during pregnancy: HA-native ewes (group HH) and ewes native to lowlands but moved to HA immediately after the diagnosis of pregnancy (group LH). A control group (LL) was kept at sea level. Near term, placentomes were removed, weighed, and processed for immunohistochemical detection of VEGF and eNOS, as well as for vascular area measurement. Placental weight was significantly higher in the HH group than in the LH and LL groups; between the latter 2 groups there was no significant difference. The placental area occupied by vasculature was significantly greater in both the HA groups than in the LH group; the number of placentomes was greatest in the LL group. The density of VEGF and eNOS in the placentome tissue was significantly greater in both HA groups than in the LL group. Although the density of VEGF was significantly lower in the HH group than in the LH group, no differences were observed in eNOS density between the HH and LH animals. These results demonstrate that chronic hypoxia upregulates the expression of placental VEGF and eNOS, suggesting an important role of these molecules in the placental response to HA hypoxia. In addition, an attenuated response to hypoxia in VEGF synthesis may be part of the long-term process of adaptation to HA.
Afficher plus [+] Moins [-]Expression of vascular endothelial growth factor and endothelial nitric oxide synthase is increased in the placenta of sheep at high altitude in the Andes Texte intégral
2010
Parraguez Gamboa, Víctor | Miljenko, Atlagich | Urquieta, Bessie | Galleguillos, Marco | Reyes Solovera, Mónica de los | Kooyman, David L. | Araneda, Silvia | Raggi Saini, Luis
Artículo de Publicación ISI | Fetal weight and the placenta of sheep at high altitude (HA) are affected by hypoxia. Placental changes (an increase in placental size and vascularization) are greater in ewes from populations that have lived for several generations at HA than in those exposed during just 1 gestation. This study investigated placental expression of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS), 2 molecules involved in placental angiogenesis that could be upregulated by hypoxia. Two groups of ewes were maintained at HA (3589 m) during pregnancy: HA-native ewes (group HH) and ewes native to lowlands but moved to HA immediately after the diagnosis of pregnancy (group LH). A control group (LL) was kept at sea level. Near term, placentomes were removed, weighed, and processed for immunohistochemical detection of VEGF and eNOS, as well as for vascular area measurement. Placental weight was significantly higher in the HH group than in the LH and LL groups; between the latter 2 groups there was no significant difference. The placental area occupied by vasculature was significantly greater in both the HA groups than in the LH group; the number of placentomes was greatest in the LL group. The density of VEGF and eNOS in the placentome tissue was significantly greater in both HA groups than in the LL group. Although the density of VEGF was significantly lower in the HH group than in the LH group, no differences were observed in eNOS density between the HH and LH animals. These results demonstrate that chronic hypoxia upregulates the expression of placental VEGF and eNOS, suggesting an important role of these molecules in the placental response to HA hypoxia. In addition, an attenuated response to hypoxia in VEGF synthesis may be part of the long-term process of adaptation to HA. | The authors thank Gabino Llusco and Enrique Perez for assistance with animal management and care. This study was funded by grants ENL 06/2 (from Direction of Research and Development, DID, University of Chile) and FONDECYT 1070405 (from National Agency for Scientific Research, CONICYT).
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