Babesiosisis a tick borne hemoprotozoan disease of canines and anaemia is the predominant clinical syndrome. This disease causes considerable morbidity and mortality in canines. Several factors such as leukocyte activation, vascular endothelial cell injury and release of chemical mediators lead to multi-organ failure and coagulation abnormality in complicated form of babesiosis. The assessment of these biomarkers is used as indicators of severity of disease and outcome in dogs with MODS in babesiosis. Successful treatment of babesiosis is challenging task and no drugs have been demonstrated to clear B. gibsoni (Asian genotype) infection. Newer successful treatment has shown encouraging response in cure of B. gibsoni infection which includes combination of clindamycin, metronidazole and doxycycline.

Six cattle presented with history and clinical signs pertaining to intestinal disorders requiring surgical intervention were subjected to left flank laparotomy. The animals were clinically examined and stabilized with fluid therapy before performing exploratory laparotomy. Left flank laparotomy was performed under Farquharson’s paravertebral nerve block in standing position. The efficacy of surgery through left flank to intestinal affections was evaluated by ease of approach, accessibility and visceral pain. It was found that caudal left flank approach allowed more ease of exteriorization of intussuscepted masses that were in jejunum, jejuno-jejunum, ileum and ileo-caecal parts of the intestine and also allowed complete exploration of the abdominal cavity. Left flank laparotomy approach with or without rumenotomy resulted in more area of exploration, prevented the proximal distended intestines to spill out and specific exteriorisation of the affected segment, by which mesenteric pain was alleviated that otherwise leads to recumbency and kicking at abdomen by the animals which could result in multiple rupture of intestines.

A study was conducted to evaluate external and internal egg quality characters of three chicken breeds/ ecotypes namely, indigenous Siruvidai, TANUVAS Aseel and White Leghorn.  A total of 180 eggs (120 eggs from Siruvidai chicken, 30 eggs from TANUVAS Aseel and 30 eggs from White Leghorn) were subjected to this study. The weight, length, width and surface area of White Leghorn eggs were significantly (P<0.05) higher than those of TANUVAS Aseel and indigenous Siruvidai chicken. Shape index was found to be significantly (P<0.05 higher in Siruvidai chicken eggs compared to that of TANUVAS Aseel and White Leghorn. Among the internal egg qualities, the height and width of albumen and albumen index were significantly (P<0.05) higher in White Leghorn chicken eggs compared to that of TANUVAS Aseel and Siruvidai chicken. Yolk height and yolk index of three breeds showed no significant difference among breeds. Yolk width was significantly (P<0.05) higher in TANUVAS Aseel compared to that of Siruvidai chicken. Yolk colour was significantly (P<0.05) higher in TANUVAS Aseel and Siruvidai chicken compared to that of White Leghorn. Shell thickness was significantly (P<0.05) higher in TANUVAS Aseel and White Leghorn as compared to Siruvidai chicken. Haugh unit was significantly (P<0.05) higher in White Leghorn compared to TANUVAS Aseel and Siruvidai chicken. The results revealed that the egg quality characters like egg weight, egg length, egg width, shape index, surface area, albumen height, albumen index, shell thickness and Haugh unit of indigenous Siruvidai chicken is different from other indigenous breed like Aseel and exotic breed like White Leghorn.

The present study was conducted to assess the efficacy of two oestrus induction protocols on 30 postpartum anoestrus crossbred cows reared by rural farmers of Puducherry region. The cows were randomly divided into Treatment I (CIDR+PGF2α+GnRH; n=15) and Treatment group II (Ovsynch+ CIDR; n=15) with double fixed timed inseminations at 12 and 24 hours post-administration of last GnRH. Oestrus induction response was 100% in both the treatment groups with majority of the cows (80%) in both the treatment groups showed intermediate intensity of oestrus. The mean time to onset of oestrus from the time of removal of CIDR in Treatment I and Treatment II was 43.45 ± 9.21 and 45.81 ± 6.34 h respectively. There is no significant difference in the serum progesterone concentration at the time of AI between Treatment I (0.92±0.26 ng/mL) and Treatment II (0.92±0.18 ng/mL). Though the conception rate was higher in treatment II (40 %) compared to Treatment I (20 %), the difference is not statistically significant. From the present study, it was concluded that Ovsynch in combination with CIDR found to be effective in oestrus induction and conception following fixed timed AI in anoestrus cows under field conditions. 

One year old male Labrador Retriever dog, was presented with the history of inability to retract the penis back into preputial sheath since 24 hours after a mounting attempt. Massage therapy, application of magnesium sulfate and ice pack failed to reduce penile oedema. Topical application of oxytocin around bulbus gland is successfully reduced oedema, thereby aided in easy repositioning. Purse-string sutures were applied around the tip of the preputial sheath and post reduction therapy followed. Animal had an uneventful recovery. 

This study presents two clinical cases in Chinchilla and Himalayan rabbits, exhibiting symptoms such as alopecia, skin lesions, and ear lesions. Skin scrapings and fecal samples were collected and processed, revealing the presence of Sarcoptes scabiei mites, Psoroptes cuniculi mites, and Eimeria oocysts. Treatment involved subcutaneous administration of Ivermectin (0.2 mg/kg), accompanied by supportive and topical application of Benzyl benzoate. The rabbits displayed clinical improvement, with complete recovery observed within 30 days.

A survey was undertaken to screen and evaluate the presence of organochlorine pesticide residue in poultry products. Both commercial and desi chicken eggs and meat samples were collected from Namakkal area of Tamil Nadu and analyzed by thin layer chromatography and confirmed by gas chromatography- mass spectrometry. Total Endosulfan in commercial chicken egg and in broiler thigh and breast muscle exceeded the MRL of Codex alimentarius. The occurrence was 50 percent with regards to broiler meat and 33.33 percent with regards to commercial egg. Desi chicken adipose tissue had a high incidence of endosulfan residue (58.33 per cent) with the levels of 28.57 ± 2.85 ppb. But the detected mean values were below the permissible MRL. DDT (Dichlorodiphenyltrichloroethane) was detected in broiler thigh and breast meat. The values obtained were below the MRL of Codex alimentarius and EU standards. BHC (Benzene hexa chloride) was detected in broiler liver, thigh and breast meat while the per cent incidence was higher in liver (33.33). The detected mean values were below the maximum permissible level of Codex alimentarius and EU standards. Both desi and broiler egg and meat analyzed were negative for aldrin, dieldrin and endrin residues which indicate that there may be these pesticides are not used in the geographical area from where samples were obtained. The findings of this study indicates that the presence of organochlorine pesticide residues, though at low level of incidence, still continues to be a threat for poultry products, hence it is essential to have a constant screening and monitoring of these compounds in feedstuff and feed at feed mills and mixing plants so as to prevent contamination in the eggs and meat.

Anthelmintics are used traditionally as an integral part of helminthic control strategies for grazing livestock to prevent production losses from parasitic infections. The continuous and indiscriminate use of the same anthelmintics over years together as the sole means of control are now failing due to the emergence of resistance strains of helminths. Resistance to the commonly used anthelmintics in gastrointestinal nematodes of sheep has become an increasingly wide spread problem throughout the world. The present study was aimed to determine the resistance against albendazole, fenbendazole, levamisole and closantel in gastrointestinal nematodes of sheep. Fifty five naturally infected Madras Red lambs of 6 - 12 months of age were selected and distributed randomly into five treatment groups of eleven animals each. Four groups were treated orally with albendazole (5mg/kg), fenbendazole (7mg/kg), levamisole (7.5m/kg) and closantel (10 mg/kg) respectively, while the fifth group served as untreated control.  Faecal samples were collected per rectum of each lamb just prior to treatment (pre treatment) and then on days 7, 14, 21 and 28 after treatment. The anthelmintic resistance was evaluated by in vivo faecal egg count reduction test (FECRT), post treatment larval culture and in vitro egg hatch assay. In the faecal egg count reduction test, albendazole reduced the faecal egg count by 86.50 per cent, 84.81 per cent, 85.28 per cent and 84.47 per cent respectively for four weeks after treatment. Faecal egg count reduction using fenbendazole was 92.64, 93.04, 90.80 and 90.06 per cent respectively for four weeks after treatment.  The per cent efficacy for levamisole and closantel was more than 95 per cent. The post treatment larval culture contained only Haemonchus contortus. In the in vitro egg hatch assay, the ED 50 value for benzimidazole was 0.299 µg thiabenazole/ml and levamisole showed an ED 50 value of 0.283 µg /ml.

High performance thin layer chromatographic method was developed and validated according to the protocol on “Validation of Analytical Procedures: Methodology, Veterinary International Cooperation on Harmonization (VICH)” with respect to linearity, sensitivity, precision and accuracy for determination of aflatoxin B1 in feed ingredients and feed. Chromatography was performed on thin layer chromatography (TLC) silica gel 60F254, aluminum sheets by Camag Linomat-5 applicator, with mobile phase condition acetone : chloroform (1 : 9). Analysis of samples viz. feed ingredients and feed, for aflatoxin B1was carried by HPTLC method and compared with TLC method. Extraction of aflatoxin was done as per AOAC method with screening and quantification by TLC and further quantification by HPTLC using reference standards. Out of 38 samples of nine types of feed ingredients analysed, samples of Bengal gram and rice bran & wheat bran mixture were negative by both methods. The other ingredients like cumbu/bajra, de-oiled rice bran, groundnut oil cake, maize, soyabean meal and sunflower oil cake, by HPTLC method wherein the Aflatoxin B1was found to be ranging from 1.61 ppb to 630.73 ppb of 77.42% positive samples, whereas by TLC method it was from 05 ppb to 140 ppb in 70.97% positive samples. While 4 samples of wheat bran analysed were all negative for Aflatoxin B1 by TLC method, whereas 50% (2 samples) found to be positive with HPTLC method with concentration ranging from 2.73 to 17.88.Similarly out of 59 feed samples analysed, 47 and 46 samples were positive for Aflatoxin B1 representing 79.66% and 77.97% of the samples, with concentration ranging from 0.54 ppb to 204.72 ppb and from 05 ppb to 710 ppb by HPTLC and TLC respectively. In the present study, the Limit of detection by HPTLC was 0.5 ppb whereas it was 5 ppb with TLC method.

A total of 30 maize samples, 30 deoiled rice bran (DORB), 20 groundnut oil cake (expeller) and 20 dried distillers’ grain soluble (DDGs) feeds samples were collected from different parts of Namakkal district. Aflatoxin B1 was estimated in all the samples by extracting the aflatoxin and spotted in an activated thin layer chromatography (TLC) plate with standards and ascertained the concentration by visual comparison method in a UV viewing cabinet. Among 30 samples of maize, analyzed for aflatoxin, 20 samples (60%) contained traces of aflatoxin and 8 samples contained between 10-30 ppb. Two samples contained between 50-100 ppb. Similarly, among 30 samples of DORB analyzed for aflatoxin, 24 samples contained traces of aflatoxin and five samples contained between 10-30 ppb. One sample contained between 50-100 ppb of aflatoxin. Three samples of GNC and Four samples of DDGS had 50-100 ppb of aflatoxin. It reveals that, very few samples of maize and DORB contained high level of aflatoxin. Hence the regular screening of toxins in every lot of feed prior to feeding the animals or poultry needs to be regularized.