Affiner votre recherche
Résultats 211-220 de 860
Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow Texte intégral
2018
Snarska, Anna | Wysocka, Dominika | Rytel, Liliana | Makowska, Krystyna | Gonkowski, Sławomir
Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow Texte intégral
2018
Snarska, Anna | Wysocka, Dominika | Rytel, Liliana | Makowska, Krystyna | Gonkowski, Sławomir
Introduction: Bisphenol A (BPA) is a substance widely used in industry for the production of polycarbonates and epoxy resins used in packaging and containers for beverages, contact lenses, compact discs (CDs), window panes, and many other elements. This compound belongs to the group of polyphenols and xenoestrogens commonly found in the human environment. What we know about BPA is still insufficient to enable us to protect our health against its adverse effects, and current knowledge of the influence of BPA on erythroblastic cell lines in bone marrow is rather fragmentary. The aim of the experiment was to assess the effect of two doses of BPA (0.05 mg/kg and 0.5 mg/kg b.w. per day) on myeloid haematopoiesis. Material and Methods: During this experiment, the number of all types of cells in the erythroblastic cell line was evaluated in porcine bone marrow before and after BPA administration. Results: The obtained results clearly indicate changes in haematopoietic activity of the bone marrow, which was demonstrated by a decrease in erythroblastic cell line production in both experimental groups. The haematological effects of the bone marrow changes were anaemia, caused by a number of erythrocytes which was depressed due to their immaturity, and a significant decrease in mean cellular volume in both groups. Conclusion: The harmful effect of high and low doses of BPA on haematopoietic processes was proved.
Afficher plus [+] Moins [-]Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow Texte intégral
2018
Snarska Anna | Wysocka Dominika | Rytel Liliana | Makowska Krystyna | Gonkowski Sławomir
Introduction: Bisphenol A (BPA) is a substance widely used in industry for the production of polycarbonates and epoxy resins used in packaging and containers for beverages, contact lenses, compact discs (CDs), window panes, and many other elements. This compound belongs to the group of polyphenols and xenoestrogens commonly found in the human environment. What we know about BPA is still insufficient to enable us to protect our health against its adverse effects, and current knowledge of the influence of BPA on erythroblastic cell lines in bone marrow is rather fragmentary. The aim of the experiment was to assess the effect of two doses of BPA (0.05 mg/kg and 0.5 mg/kg b.w. per day) on myeloid haematopoiesis.
Afficher plus [+] Moins [-]Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Texte intégral
2018
Bai, Ge | Li, Hui | Ge, Yansong | Zhang, Qianzhen | Zhang, Jiantao | Chen, Mingzi | Liu, Tao | Wang, Hongbin
Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Texte intégral
2018
Bai, Ge | Li, Hui | Ge, Yansong | Zhang, Qianzhen | Zhang, Jiantao | Chen, Mingzi | Liu, Tao | Wang, Hongbin
The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury. Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy. Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group. Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.
Afficher plus [+] Moins [-]Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Texte intégral
2018
Bai Ge | Li Hui | Ge Yansong | Zhang Qianzhen | Zhang Jiantao | Chen Mingzi | Liu Tao | Wang Hongbin
The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.
Afficher plus [+] Moins [-]New insight of apparently healthy animals as a potential reservoir for Clostridium perfringens: a public health implication Texte intégral
2018
Hamza, Dalia | Dorgham, Sohad M. | Elhariri, Mahmoud | Elhelw, Rehab | Ismael, Elshaimaa
New insight of apparently healthy animals as a potential reservoir for Clostridium perfringens: a public health implication Texte intégral
2018
Hamza, Dalia | Dorgham, Sohad M. | Elhariri, Mahmoud | Elhelw, Rehab | Ismael, Elshaimaa
Introduction: Clostridium perfringens is commonly found in the gastrointestinal tract of animals and humans and continues to cause one of the most prevalent foodborne diseases in man. Material and Methods: A total of 355 samples were examined for the occurrence of C. perfringens: rectal swabs from cattle, sheep, and goats, fresh stool samples from diarrhoea sufferers having been in contact with these animals, irrigation water and soil samples from the husbandry sites, and preharvesting fresh produce from farms irrigated with the sampled water. All samples were collected from Cairo and Giza governorates, Egypt. PCR analysis was carried out with positive isolates using the α-toxin gene. Sequence analysis of the gene of C. perfringens isolates was performed using the neighbour-joining approach. Bootstrap analysis was executed with 1,000 resamplings. Results: 174 C. perfringens strains were isolated with a 49.01% prevalence. The highest prevalence of C. perfringens in apparently healthy animals was found in sheep (65.45%) followed by goats (58%), buffaloes (55%), and cattle (47.1%). Its prevalence in humans being in contact with these animals was 47.5%. The bacterium’s isolation from the soil and irrigation water was achieved in 40% and 31.7% of samples, respectively, posing a risk, particularly when the water and soil contact food in the field, shown by the fresh produce isolation of 40%. A significant relationship between the prevalence of C. perfringens in animal and environmental samples was identified (P < 0.05). A significant relationship was identified neither between animal species and C. perfringens prevalence, nor between the environmental source and C. perfringens prevalence (P > 0.05). All isolates were positive for the α-toxin gene by PCR. The sequence analysis and the phylogenetic relationship of the α-toxin genes from different samples revealed that C. perfringens from faeces of apparently healthy cattle, buffaloes, sheep, and goats is a significant threat in places where it can contaminate the soil and water. In addition, the sequence of C. perfringens from humans suffering from diarrhoea was found in the same cluster with the sequence from cows, goats, and sheep. Conclusion: The role of apparently healthy animals in transmitting C. perfringens to humans, either through being in direct or indirect contact via water or soil in the cultivation of vegetables and fruits, was demonstrated.
Afficher plus [+] Moins [-]New insight of apparently healthy animals as a potential reservoir for Clostridium perfringens: a public health implication Texte intégral
2018
Hamza Dalia | Dorgham Sohad M. | Elhariri Mahmoud | Elhelw Rehab | Ismael Elshaimaa
Introduction:Clostridium perfringens is commonly found in the gastrointestinal tract of animals and humans and continues to cause one of the most prevalent foodborne diseases in man.
Afficher plus [+] Moins [-]Perikarditis Travmatikalı Sığırlarda Venöz Kan Gaz Ve Asid- Baz Analizlerin Önemi Texte intégral
2018
YILDIZ, Ramazan
Yapılan çalışma Perikarditis Travmatika (PT) olan sığırların rutin klinik muayeneleri esnasında alınan venöz kan gazlarınınincelenmesi sonucu oluşturuldu. Bu çalışmanın amacı kan gazı analizinin Perikarditis Travmatikalı sığırlarda önemini ortayakoymaktır. Çalışmada Perikarditis Travmatika tanısı koyulan 22 adet 2-4 yaş aralığında olan sığır kullanıldı. Kan gaz analizleriiçin V. Jugularisten 2,5 ml kan alınarak 15 dk. içinde kan gazı cihazında analiz edildi. Yirmi sığırın kan pH'sı normal (7.35-7.50)sınırlarında iken, bir hayvanda hafif asidemi (7.20-7.35), 1 hayvanda ise metabolik alkaloz ( > 7.50) tespit edildi. Kan pCO2değeri bir hayvan ( > 50 mmHg) haricinde normal referans (30-40 mmHg) alarığında olduğu görüldü. Kan pO2 değeri 11 hayvanda< 30 mmHg seviyesininnin altında olduğu tespit edildi. Laktat seviyesi 8 hayvanda normal sınırlarda (< 2 mmol/L) iken,5 hayvanda klinik uyarı (2-4 mmol/L) seviyesinde, 9 hayvanda kritik seviyenin (>4 mmol/L) üzerinde olduğu görüldü. Potasyumseviyesinin 17 hayvanda normal sınırların (<3.8 mmol/L) altındaydı. HCO3 değeri 7 hayvanda normal (20-30 mEq/L)sınırların üzerinde, 2 hayvanın ise normal sınırların altında olduğu ve bu iki hayvanda hiperlaktatemi olduğu görüldü. Yükseklaktat laktat ile düşük SatO2 ve pO2 parametreleri PT'li sığırlarda doku hipoksisi şekillendiğini göstermektedir. Sonuç olarakkan gazı ölçümünün hastanın tedavi seçenekleri ve prognostik takip için kullanılmasının anlamlı olabileceği kanısına varıldı.
Afficher plus [+] Moins [-]Yüksek Düzeyde Konsantre Yemle Beslenen Kuzularda Yeme Maya (Saccharomyces cerevisiae) veya Malik Asit İlavesinin Performans Üzerine Etkisi Texte intégral
2018
ERDOĞMUŞ SÜER, İnci Nathalie | KOCABAĞLI, Neşe
Bu çalışmada, yüksek düzeyde konsantre yemle beslenen Kıvırcık kuzularda yeme maya (Saccharomyces cerevisiae-Levabon Rumen E; Biomin,Avusturya) veya malik asit (Rumalato®; Norel & Nature S.A., Barselona, İspanya; disodyum malat ve kalsiyum malat içermektedir(0,16:0,84, w/w)) ilavesinin performans üzerine etkisinin araştırılması amaçlanmıştır. Araştırmada 3 aylık yaşta 45 baş Kıvırcık ırkı erkek kuzukullanılmıştır. Hayvanlar ağırlıkları göz önünde bulundurularak, gruplar arasında istatistiksel olarak bir fark olmayacak şekilde biri kontrol,diğer ikisi deneme olmak üzere 3 gruba ayrılmıştır. Kontrol grubunun konsantre yemlerine hiçbir katkı maddesi eklenmemiş, deneme gruplarındanbirine 1,5 g/kg KM düzeyinde canlı maya kültürü, diğerine ise 5,0 g/kg KM malik asit tuzu ilave edilmiştir. Hayvanlar 60 gün süreile besiye alınmıştır. Bu süre boyunca hayvanların sürekli olarak temiz içme suyuna erişimleri olmasına dikkat edilmiş ve ad libitum olarak(%80: %20) konsantre yem:kuru ot karması ile beslenmişlerdir. Besi süresince kuzuların canlı ağırlıkları denemenin 0, 15, 30, 45 ve 60. günlerindesabah aç karnına yapılan tartımlarla saptanmış, verilen yemler her gün, artan yemler ise haftalık olarak tartılmıştır. Deneme sonundaher gruptan 7 baş kuzu kesilerek sıcak ve soğuk karkas ağırlıkları ile karkas randımanları saptanmıştır. Yem tüketimi ve yemden yararlanmaoranları grup beslemesi yapıldığı için istatistiksel olarak değerlendirilmemiştir. Canlı ağırlık ve günlük canlı ağırlık artışlarında gruplar arasındaönemli bir fark gözlenmemiştir (p > 0,05). Sıcak ve soğuk karkas ağırlıkları ve karkas randımanları gruplar arasında istatiksel açıdan anlamlıfark göstermemiştir. Sonuç olarak, bu çalışmada kuzularda yeme maya veya malik asit ilavesi performans üzerine istatistiksel olarak önemlibir etki göstermemiştir.
Afficher plus [+] Moins [-]Kaynak Alabalığının Salvelinus fontinalis Semeninin Bakteriyel Florasının Belirlenmesi Texte intégral
2018
Ertekin, Özlem | Kutluyer, Filiz | Kocabaş, Mehmet | Başçınar, Nadir
Experiments were designed to evaluate bacterial flora in semen culture of brook trout (Salvelinus fontinalis). Herein, semen samples werecollected from fish and standard microbiology techniques were processed for bacterial flora. The samples were serially diluted in physiologicalsaline (NaCl: 0.85%). Aliquots of 0.1 ml of each dilution were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose BengalAgar (RBC) (Yeast-Mold Count), VRBD (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count).Micrococcus/Staphylococcus group bacteria (11.11%) were counted from brook trout (S. fontinalis) semen. Total bacteria count varied between103-105 cfu ml-1 in semen.
Afficher plus [+] Moins [-]Comparative Toxicity of Paraquat and 2, 4-Dichlorophenoxy Acetic Acid in Adult Artemia franciscana Texte intégral
2018
Rahnama, Roya | Tulaby Dezfuly, Zahra | Alishahi, Mojtaba
Herbicides are being used widely in agriculture and aquaculture for controlling noxious weeds. Paraquat and 2, 4-Dichlorophenoxyacetic acid(2,4-D) have been the most widely used herbicide during the past three decades. Toxicological properties of Paraquat are attributed to its abilityto produce reactive oxygen species such as superoxide anion that may directly or indirectly cause cell death. 2,4-Dichlorophenoxyacetic acid(2,4-D) is a broad-leaf, systemic, phenoxy herbicide used as the active ingredient in several commercially available aquatic herbicide products.Bioassay technique has been the cornerstone of programs on environmental health and chemical safety. The application of environmentaltoxicology studies on non-mammalian vertebrates is rapidly expanding. So the present study investigated the acute toxicity of Paraquat and2,4- Dichlorophenoxy acetic acid (2,4-D) as aquatic ecosystems pollutants on Artemia franciscana. Artemia is one of the most suitable testorganisms available for ecotoxicity testing and research and most commonly used live food in aquaculture. Acute toxicity (48 h LC50) of twoherbicides (Paraquat, 2, 4-dichlorophenoxy acetic acid) was determined. A. franciscana exposed to Serial concentrations of both mentionedherbicides. Mortalities at 12, 24, 36 and 48 hours after exposure were recorded and LC50 were calculated using Probit software. The resultsobtained indicate that the acute toxicity of these herbicides is significantly different in adult A. franciscana. The lethal concentration of Paraquatand 2,4-D were calculated 2.701, 14.475 mg/L in A. fransiscana respectively. So The LC50 of two examined herbicides was significantly differentand the mortality rate was increased by increasing exposure time. Finally, these data support the hypothesis the possible risks associatedwith the presence of herbicides particularly Paraquat residues in the aquatic animals and their environment.
Afficher plus [+] Moins [-]Concentration Of Cortisol Metabolites In Captive Sumatran Elephants At Elephant Conservation Facilities In Aceh Texte intégral
2018
Wahyuni, Rosa Rika | Wahyuni, Sri | Azhar, Al | Gholib, Gholib
This study was conducted to validate the DRG Cortisol ELISA EIA-1887 Germany kit for measure the concentration of stress hormone metabolites (cortisol) from the feces and its correlation to the stressor factor in captive elephants in PKG and CRU of Aceh. These factors are location, diet and presence of livestock. There is no special treatment, observation based on the activity, behavior or natural condition of the animals. The sampling technique was non invasive, fresh dung samples of each (20 gram) were collected from 25 elephants in CRU and PKG. Feces taken in the morning (before the animals are bathed) along with the observation of animal behavior. All samples were collected and stored at -200C until the analysis process. The validation test are analytic (parallelilmsm) and biological validation test. The analytic test result (paralillsm), showed that the sample curve was not parallel to the standard curve, but crossed the standard curve. While the results of biological validation test, DRG Cortisol ELISA EIA-1887 Germany kit can measure the concentration of cortisol hormone feces of Sumatran elephant and able to describe the difference of cortisol concentration relation to physiological events (stress vs non-stress). The mean values of cortisol metabolite concentrations from PKG Saree (Komplek PKG and Hutan Seunapet), Sampoiniet CRU, Cot Girek, Das Peusangan, Meulaboh and Aceh Timur were (577 ng/g and 400 ng/g), 435ng /g, 419ng /g, 517ng / g, 401ng/g and 425ng /g. The measurement results correlate with the physiological conditions and observed factors.
Afficher plus [+] Moins [-]Effectivity of Entomopathogen of Fungi of Metarhizium brunneum Petch As Biotermitiside for Termite Pest of Coptotermes curvignathus Holmgren on Nutmeg in Aceh Province Texte intégral
2018
Sayuthi, Muhammad | Santoso, Teguh | Iswadi, Iswadi
Abstract
Afficher plus [+] Moins [-]Management Femoral Fracture in Cats using Intramedullary Pin and Wires Fixation Texte intégral
2018
Erwin, Erwin | Noviana, Deni | Umbu, Dany | Dewi, Tri Isyani Tungga
On April 2016, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Bogor Agricultural University (VTH FKH IPB) received 4 cases of femoral fractures in cats caused by trauma. Two cats suffered oblique diaphysis fracture femoral, one cat suffered comminuted diaphysis fracture femoral, and one cat suffered epiphyseal fracture femoral (column femoral and trochanter major). All cats were treated by open surgery using intramedullary pins (Steinmann) and cerclage wires (Kirschner). Fracture recovery went without complication and all cats were able to move their feet one week after surgery.
Afficher plus [+] Moins [-]