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A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.

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dna cattle genetics cattle animals nucleic acid hybridization viral isolation & purification probes (equipment) southern blotting malignant catarrh ...

National Agricultural Library - United States of America

Alterations in epinephrine-induced arrhythmogenesis after xylazine and subsequent yohimbine administration in isoflurane-anesthetized dogs

1988

Tranquilli, W.J. | Thurmon, J.C. | Benson, G.J.

Effects of xylazine (1.1 mg/kg of body weight, IV bolus, plus 1.1 mg/kg/h infusion) and subsequent yohimbine (0.125 mg/kg, IV bolus) administration on the arrhythmogenic dose of epinephrine (ADE) in isoflurane (1.8% endtidal)-anesthetized dogs were evaluated. The ADE was defined as the total dose of epinephrine that induced greater than or equal to 4 premature ventricular contractions within 15 seconds during a 3-minute infusion period or within 1 minute after the end of infusion. Total ADE values during isoflurane anesthesia, after xylazine administration, and after yohimbine injection were 36.6 +/- 8.45 micrograms/kg, 24.1 +/- 6.10 micrograms/kg, and 45.7 +/- 6.19 micrograms/kg, respectively. Intravenous xylazine administration significantly (P less than 0.05) increased blood pressure and decreased heart rate, whereas yohimbine administration induced a significant (P less than 0.05) decrease in blood pressure. After yohimbine administration, the ADE significantly (P less than 0.05) increased above that after isoflurane plus xylazine administration. After yohimbine administration, blood pressure measured immediately before epinephrine-induced arrhythmia was significantly (P less than 0.05) less than the value recorded during isoflurane plus xylazine anesthesia. Heart rate was unchanged among treatments immediately before epinephrine-induced arrhythmia. Seemingly, yohimbine possessed a protective action against catecholamine-induced arrhythmias in dogs anesthetized with isoflurane and xylazine.

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anesthesia xylazine yohimbine animals blood pressure heart rate isoflurane thiazines female drug effects inhalation male ...

National Agricultural Library - United States of America

Role of maternal antibody in determination of an effective newcastle disease vaccination programme

1988

Saeed, Z. | Ahmad, S. (Veterinary Research Inst., Lahore) | Rizvi, A.R. | Ajmal, M. (College of Veterinary Sciences, Lahore (Pakistan))

enfermedad de newcastle newcastle disease broiler chickens pollo de engorde poulet de chair maladie de newcastle immunisation/ anticorps immunization/ antibodies inmunizacion/ anticuerpos

National Agricultural Research Centre - Pakistan