The plant Limonia acidissima belongs to family Rutaceae. Various parts of this plant like fruit, seed, rind, bark, leaves and roots are being used to treat human and animal ailments. The fruits of the plant are most essential therapeutic portion containing bioactive molecules. This study is intended to reveal presence of some phytoconstituents from leaf extracts and review it thoroughly in order to validate its medicinal use. In the study it was found that aqueous and ethanolic extracts revealed maximum types of phytochemical classes as compared to acetone and petroleum ether extract which can be used for treatment with reference to its mentioned use. Many phytochemicals namely alkaloids, carbohydrates, glycosides, proteins, saponins, phenols, resins, etc. were detected which  reflects its therapeutic value.

Contamination of locally fermented cow milk (Nono) by pathogenic bacteria constitute a major public health problem. This was because contaminated milk was a good medium that can support the growth and propagation of microbial pathogens. This study was designed to assess the bacteriological quality of locally fermented cow milk consumed in Maiduguri, northeastern Nigeria. Totally, forty (n = 40) samples of fresh, locally fermented cow milk (Nono) were collected and used in this study. Ten (10) milk samples each were collected from four randomly selected selling points (Monday Market, Custom Market, Kasuwan Shanu and Tashan Bama). The bacteriological quality of each milk sample were analysed using standard procedures for isolation, identification and enumeration of pathogenic bacteria. The mean total plate count ranged from 1.44x10 8 CFU/mL to 10.31x10 8 CFU/mL, while the mean total coliform count range from 0.57x10 8 CFU/mL to 11.17x10 8 CFU/mL. Statistically, there was a significant difference (p<0.05) in the mean total plate count and coliform count of milk samples collected from the four sampling points.Bacteriological culture and biochemical identification of all the isolates revealed Escherichia coli29(72.5%), Klebsiella specie3(7.5%), Salmonella specie 2(5%), Staphylococcus aureus 14(35%) and Streptococcus specie15(37.5%) respectively. All the results were above the acceptable limits (1.0 to 3.0 x 10 5 CFU/mL for total plate count and no coliform count per 100ml of milk sample) set by NAFDAC. Therefore the Nono products consumed in Maiduguri was not safe and wholesome for public consumption. Hence, the need to ensure the maintenance of standard hygienic protocols during the collection, storage, processing and marketing of locally fermented cow milk (Nono) in order to minimize microbial contamination.

Drug interaction is the effect produced by combination of various drugs administered in a biological system which may produce beneficial or adverse effects.Due to continuous usage of antibiotics to bacterial infections, pathogenic bacteria developed resistance mechanisms which leads to reduced antibacterial activity of different antibiotics. Combination of antibiotics with phytochemicals became an alternative to enhance the efficacy of antibiotics. In this connection,in the present study, the effect of certain phytochemicals namely Quercitin, Morin, Ellagic acid,  Chloragenic acid Rutin and Naringenin in combination with antibiotics Amoxycillin and Ciprofloxacin were evaluated. Staphylococcus aures and Escherichia coli were used as test organisms. They were isolated from the mastitis milk samples and their presence was confirmed using Polymerse Chain Reaction .The antibacterial activity of the antibiotics and antibiotics in combination with phytochemicals were determined using broth dilution method and agar well diffusion assay. The MIC and zones of inhibition were used as indicators for drug interaction.

Milk is one of the primary protein diets of high biological value available to the human population. As bovine beta casein gene is highly polymorphic with functional implications, this study was designed to detect the beneficial A2 genotype in the native Umbalachery cattle breed. Genomic DNA was isolated from blood samples of Umbalachery cows and A1 and A2 variants were detected by Tetra ARMS polymerase chain reaction. It was observed that the A2A2 genotype frequency was 82% and that of A1A2 was 18%. The A1A1 genotype was not detected in Umbalachery cattle in the present study. It may be concluded that majority of the Umbalachery cows retain their original beta casein protein variant that produce safer A2 milk for human consumption and that Tetra ARMS PCR is an efficient and rapid method that allows detection of single nucleotide polymorphism of A1 and A2 variants of beta casein gene.

Physico-chemical parameters of fresh milt and mitochondrial activity of fresh spermatozoa of the endangered golden mahseer, Tor putitora (Hamilton) were investigated. Cryopreservation and fertility evaluation of cryopreserved-thawed spermatozoa of T. putitora were carried out. The concentration of spermatozoa and the spermatocrit values of the fresh milt were estimated to be 3.96±0.12 x 107 spermatozoa/ml and 70.00±1.53% respectively. The motility percentage of spermatozoa in the fresh milt was estimated to be 94.84±0.43. The elemental composition analysis revealed that the concentration of K+ was higher i.e., 14.13±0.32 mg/l when compared to that of Na+, Ca+, Mg+ and Zn+. The concentration of total reducing sugars and total proteins were estimated to be 51.79±0.47mg/100 ml and 40.57±0.75 mg/100ml respectively. The absorbance value of the fresh spermatozoa in Sperm Mitochnodrial Activity Index (SMAI) assessment by Nitro Blue Tetrazolium (NBT) assay was estimated to be 0.33±0.012. The optimum sperm: egg ratio was estimated to be 3.96 ±0.12 x 104+ no./egg. Hatching percentage of 45.97±1.72 was recorded for the cryopreserved-thawed  spermatozoa while the fresh milt control recorded a hatching percentage of 73.10±0.82.

 The experiment was conducted to assess the effect of replacement of maize by wheat bran on growth and feed conversion efficiency in Large White Yorkshire (LWY) pigs. Thirty weaned piglets were randomly divided into three groups and were allotted to the three dietary treatments, T1 (control ration as per NRC, 1998), T2 (50 per cent of maize of control ration replaced by wheat bran) and T3 (100 per cent of maize of control ration replaced by wheat bran) and maintained for 70 days. The daily feed intake, fortnightly body weight were recorded and average daily gain and feed conversion efficiency were calculated. The average daily gain and feed conversion efficiency were 813.00, 769.86, 678.43 g and 2.80, 2.88, 3.08, respectively for three dietary treatments. There was no significant difference in the performance between pigs belongs to T1 and T2 treatments but had higher average daily gain and feed conversion efficiency than that of T3 treatment. This study reveals that pigs fed diet with 50 per cent of maize replaced by wheat bran performed in a similar way to that of control fed group and was better than those fed diet with 100 per cent replacement of maize by wheat bran.

The present study was undertaken to induce gonadal maturation of Mrigal, Cirrhinus mrigala using Human Chorionic Gonadotropin (HCG) implants in captive conditions. The male and female fishes of C. mrigala were implanted intramuscularly with hCG implants at the dosage of 1000 IU/kg body weight, to study the effect of hCG hormone. Hormone implants were given once in two months during the experimental period of February to June. Histological observation in the ovary of C. mrigala revealed the presence of four types of growing oocytes namely perinucleolar, previtellogenic, vitellogenic and hydrated oocytes. Histological observation of the testis of C. mrigala showed the presence of five types of spermatogenic cell types namely spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. Hence, the present study recommends that hCG implants can successfully be used to induce sustained maturation of C. mrigala in captivity even during off season. 

The prime objective of the study was to analyze the ecological features and to identify the fish species present along the upstream region of Pechipparai reservoir. Seventeen species of freshwater fishes belonging to 11 genera and 8 families have been recorded. Cyprinidae was the dominant family (6 species) in the study area. Of the 17 species, Dawkinsia arulius belong to the endangered category, Hyporhamphus xanthopterus and Dawkinsia rohani to vulnerable and Ompok bimaculatus to near threatened category. All the 17 fish species are found to be endemic to India and ornamentally appreciated.

The morphology of the abducent nucleus of the buffalo has been described by materials collected from eight buffalos. Nissl and myelin stained serial and semi serial sections of brain stem were used for the study. The abducent nucleus in the buffalo extended from the cranial pole of the rostral cochlear nucleus to the caudal pole of the motor nucleus of the trigeminal nerve. An accessory abducent nucleus which is present in amphibians, reptiles, birds and some mammals could not be identified in the buffalo. The nucleus appeared as a rounded body in its caudal pole and was roughly triangular at rostral pole in transverse sections. The average length of motor nucleus of abducent nerve in the buffalo was 2.4 mm. The average maximum width and height of the nucleus was 1.48mm and 1.75 mm respectively.

The current study was designed to evaluate the influence of three different ground vegetable oil seeds on various physico-chemical, microbiological and sensory attributes of chicken sausages from spent broiler breeder hen stored under frozen (-18±1°C) condition. Chicken sausages were incorporated with ground poppy, sesame and peanut seeds each at 10 % level separately as three treatments and were analyzed for their keeping quality along with high fat control. The mean values of FFA, TBARS, tyrosine values, standard plate count, psychrophilic count and yeast and moulds counts were significantly (P<0.05) influenced by formulation during frozen storage and values increased significantly (P<0.05) throughout the storage period in all chicken sausages. Chicken sausages with ground sesame scored lowest standard plate counts (5.66 log CFU/g) than any others after 180 days of storage at 18±1°C. Coli forms, lacto bacillus and anaerobic counts could not be detected during entire storage period. Scores for all sensory attributes decreased significantly (P<0.05) during storage in all treatments irrespective of formulation. Study proved that, the three ground vegetable oil seeds were effective by delaying the lipid oxidation, microbial growth and preserving sensory quality during frozen storage and among them ground sesame seed was more potent to be a better preservative.