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Prevalence of mastitis in dairy cows from smallholder farms in Zimbabwe Texte intégral
2013
Davies M. Pfukenyi | Masimba Ndengu | Gift Matope | Simbarashe Katsande
Prevalence of mastitis in dairy cows from smallholder farms in Zimbabwe Texte intégral
2013
Davies M. Pfukenyi | Masimba Ndengu | Gift Matope | Simbarashe Katsande
A cross-sectional study was conducted to determine the prevalence of sub-clinical and clinical mastitis and the associated factors in cows from selected smallholder dairy farms in Zimbabwe. Physical examinations were conducted on all lactating cows for evidence of signs of clinical mastitis. Composite milk samples were collected from all lactating cows for bacterial culture and somatic cell counting. Cows were categorised as clinical if they exhibited clinical features of mastitis, or sub-clinical if no apparent signs were present but they had a positive bacterial isolation and a somatic cell count of at least 300 x 103 cells/mL. Farm-level factors were obtained through a structured questionnaire. The association of mastitis and animal- and herd-level factors were analysed using logistic regression. A total of 584 animals from 73 farms were tested. Overall, 21.1%(123/584) had mastitis, 16.3%(95/584) had sub-clinical mastitis and 4.8% (28/584) had clinical mastitis. Herd-level prevalence was 49.3%. Coagulase-negative staphylococci (27.6%), Escherichia coli (25.2%), Staphylococcus aureus(16.3%), Klebsiella spp. (15.5%) and Streptococcus spp. (1.6%) were the most common isolates. In individual cows, pure dairy herds (OR = 6.3) and dairy crosses (OR = 3.1) were more likely to have mastitis compared to Mashona cows. Farms that used pre-milking teat dipping were associated with reduced mastitis prevalence. Further research is needed on the prevalence of mastitis and a comparison of data for both smallholder and commercial dairy farms in all regions of Zimbabwe should be undertaken.
Afficher plus [+] Moins [-]Prevalence of mastitis in dairy cows from smallholder farms in Zimbabwe Texte intégral
2013
Katsande, Simbarashe(University of Zimbabwe Department of Paraclinical Veterinary Studies) | Matope, Gift(University of Zimbabwe Department of Paraclinical Veterinary Studies) | Ndengu, Masimba(University of Zimbabwe Department of Paraclinical Veterinary Studies) | Pfukenyi, Davies M(University of Zimbabwe Department of Paraclinical Veterinary Studies)
A cross-sectional study was conducted to determine the prevalence of sub-clinical and clinical mastitis and the associated factors in cows from selected smallholder dairy farms in Zimbabwe. Physical examinations were conducted on all lactating cows for evidence of signs of clinical mastitis. Composite milk samples were collected from all lactating cows for bacterial culture and somatic cell counting. Cows were categorised as clinical if they exhibited clinical features of mastitis, or sub-clinical if no apparent signs were present but they had a positive bacterial isolation and a somatic cell count of at least 300 x 10³ cells/mL. Farm-level factors were obtained through a structured questionnaire. The association of mastitis and animal-and herd-level factors were analysed using logistic regression. A total of 584 animals from 73 farms were tested. Overall, 21.1% (123/584) had mastitis, 16.3% (95/584) had sub-clinical mastitis and 4.8% (28/584) had clinical mastitis. Herd-level prevalence was 49.3%. Coagulase-negative staphylococci (27.6%), Escherichia coli (25.2%), Staphylococcus aureus (16.3%), Klebsiella spp. (15.5%) and Streptococcus spp. (1.6%) were the most common isolates. In individual cows, pure dairy herds (OR = 6.3) and dairy crosses (OR = 3.1) were more likely to have mastitis compared to Mashona cows. Farms that used pre-milking teat dipping were I: associated with reduced mastitis prevalence. Further research is needed on the prevalence of mastitis and a comparison of data for both smallholder and commercial dairy farms in all regions of Zimbabwe should be undertaken.
Afficher plus [+] Moins [-]Occurrence of Biogenic Amines in Different Types of Marketed Cheese in Gharbia Governorate Texte intégral
2013
Nesreen Z. Eleiwa | Hanan M. Lamada | Marionette Z. Nassif
This work was applied on sixty cheese samples represented by Kareish ,cheddar and Romano cheeses (20 of each).The samples were submitted to High Performance Liquid Chromatography (HPLC) for qualitative and quantitative determination of biogenic amines .The results were summarized as Kareish cheese has higher concentrations of Tyramine and Cadaverine in low and high levels of manufacturing quality ( 29.64 ± 1.72 and 9.91 ± 0.60 mg/100gm) and (17.48 ± 1.09 and 5.61± 0.37 mg/100gm) respectively, Meanwhile, Histamine level was higher in both levels of Romano cheese (22.96 ± 1.17 and 18.35± 1.12 mg/100gm) respectively. Putrescine represented in high levels in cheddar cheese (13.40 ± 1.02 and 10.61 ± 0.74 mg/100gm) respectively. Comparing with the Egyptian Organization for Standardization"EOS" (1996), all the cheese samples were not exceeded the permissible level of Cadaverine in contrast with the other biogenic amines. The study concluded that presence of high concentrations of biogenic amines reflect the bad hygienic conditions under which they produced and stored
Afficher plus [+] Moins [-]Selected haematological changes in <i>Clarias gariepinus</i> (Burchell, 1822) infected with a <i>Trypansosoma</i> sp. from the Vaal Dam, South Africa Texte intégral
2013
Maryke L. Ferreira | Annemarie Avenant-Oldewage
The use of haematological techniques to assess fish health is generally accepted. The aim of the current study was to determine selected haematological changes that occur in Clarias gariepinus (Burchell, 1822). infected with trypanosomes. Blood films were prepared according to standard techniques to confirm trypanosome infections and whole blood was collected, the serum and plasma separated, and prepared for albumin and total protein concentration analysis. Plasma albumin levels were significantly higher in infected wild caught fish than in uninfected wild caught fish and uninfected breeding stock. Serum albumin levels were significantly lower in infected wild caught fish when compared to uninfected breeding stock. The total plasma and serum protein levels were within the normal range for C. gariepinus, that is, 3 g – 6 g/100 mL. The total plasma protein levels varied significantly between the three groups. However, the total serum protein levels were only significantly different between uninfected breeding stock and uninfected wild caught fish, as well as uninfected breeding stock and infected wild caught fish. The total protein levels were significantly higher in infected wild caught fish than in the other groups, a possible indication of an infection or inflammatory host response.
Afficher plus [+] Moins [-]Histomorphometrical and ultrastructural study of the effects of carbendazim on the magnum of the Japanese quail (<i>Coturnix coturnix japonica</i>) Texte intégral
2013
Wahabu H. Kimaro | Mary-Catherine Madekurozwa | Herman B. Groenewald
The study investigated the effect of various doses of carbendazim on the morphology of the magnum of the Japanese quail. No morphological changes were observed in the magnum in birds treated with carbendazim at doses of 25 mg/kg and 100 mg/kg bodyweight. A carbendazim dose of 400 mg/kg bodyweight was the lowest dose which caused morphological changes in the magnum. Histologically, carbendazim caused pyknosis and glandular atrophy in the magnum mucosa. Carbendazim also caused significant decreases in the height of the mucosal folds, epithelial height, glandular width and glandular luminal diameter at 400 mg/kg and 800 mg/kg (p < 0.05). At ultrastructural level, dose-dependent deciliation was observed. Pyknotic nuclei, dilated cisternae of rough endoplasmic reticulum, swollen mitochondria, numerous vacuoles and lysosomes in the luminal and glandular epithelia were identified. The observed degenerative changes could be due to cytoskeletal disruption caused by carbendazim toxicity. Degeneration of the luminal and glandular cells in the magnum pose a potential threat to the egg production and reproduction of exposed birds.
Afficher plus [+] Moins [-]The incursion, persistence and spread of peste des petits ruminants in Tanzania: Epidemiological patterns and predictions Texte intégral
2013
Fredrick M. Kivaria | Olivier Kwiatek | Angolwisye M. Kapaga | Emmanuel S. Swai | Geneviève Libeau | Winford Moshy | Albano O. Mbyuzi | Joshua Gladson
The incursion, persistence and spread of peste des petits ruminants in Tanzania: Epidemiological patterns and predictions Texte intégral
2013
Fredrick M. Kivaria | Olivier Kwiatek | Angolwisye M. Kapaga | Emmanuel S. Swai | Geneviève Libeau | Winford Moshy | Albano O. Mbyuzi | Joshua Gladson
Peste des petits ruminants virus, which causes a severe disease in sheep and goats, has only recently been officially declared to be present in Tanzania. An epidemiological study was carried out between September 2008 and October 2010 to investigate the incursion, persistence and spread of the virus in Tanzania. The investigation involved serosurveillance, outbreak investigation and computation of epidemiological indices such as the effective reproductive number, persistence and the threshold level for vaccination. Field and molecular epidemiological techniques were applied to isolate, characterise and trace the origin of the virus in Tanzania. A total of 2182 serum samples from goats and 1296 from sheep from 79 villages across 12 districts were investigated. Village-level prevalence of infection was variable (0.00% – 88.00%) and was higher in pastoral than in agro-pastoral villages. The overall antibody response to the virus was 22.10% (CI 95% = 20.72% – 23.48%). About 68.00% and 73.00% of seropositive goats and sheep, respectively, did not show clinical signs. The proportion of seropositive animals differed significantly (p ≤ 0.001) between age groups, sex and farming practices. Real-time polymerase chain reaction results showed that the isolated strains belong to lineage III, whose origin is in East Africa and the Middle East. This indicates that one of the northern neighbouring countries is most likely the source of infection. The computed overall effective reproductive number, the threshold level of vaccination necessary to eradicate the disease and persistence were 4.75% and 98.00%, respectively. These estimates indicate that achieving elimination of the peste des petits ruminants virus from pastoral flocks will require significant effort and development of highly effective intervention tools.
Afficher plus [+] Moins [-]The incursion, persistence and spread of peste des petits ruminants in Tanzania: Epidemiological patterns and predictions Texte intégral
2013
Kivaria, Fredrick M.(National Epidemiology Section) | Kwiatek, Olivier(CIRAD) | Kapaga, Angolwisye M.(Central Veterinary Laboratory) | Swai, Emmanuel S.(Veterinary Investigation Centre) | Libeau, Geneviève(CIRAD) | Moshy, Winford(Veterinary Investigation Centre) | Mbyuzi, Albano O.(Veterinary Investigation Centre) | Gladson, Joshua(Central Veterinary Laboratory)
Peste des petits ruminants virus, which causes a severe disease in sheep and goats, has only recently been officially declared to be present in Tanzania. An epidemiological study was carried out between September 2008 and October 2010 to investigate the incursion, persistence and spread of the virus in Tanzania. The investigation involved serosurveillance, outbreak investigation and computation of epidemiological indices such as the effective reproductive number, persistence and the threshold level for vaccination. Field and molecular epidemiological techniques were applied to isolate, characterise and trace the origin of the virus in Tanzania. A total of 2182 serum samples from goats and 1296 from sheep from 79 villages across 12 districts were investigated. Village-level prevalence of infection was variable (0.00% - 88.00%) and was higher in pastoral than in agro-pastoral villages. The overall antibody response to the virus was 22.10% (CI95% = 20.72% - 23.48%). About 68.00% and 73.00% of seropositive goats and sheep, respectively, did not show clinical signs. The proportion of seropositive animals differed significantly (p < 0.001) between age groups, sex and farming practices. Real-time polymerase chain reaction results showed that the isolated strains belong to lineage III, whose origin is in East Africa and the Middle East. This indicates that one of the northern neighbouring countries is most likely the source of infection. The computed overall effective reproductive number, the threshold level of vaccination necessary to eradicate the disease and persistence were 4.75% and 98.00%, respectively. These estimates indicate that achieving elimination of the peste des petits ruminants virus from pastoral flocks will require significant effort and development of highly effective intervention tools.
Afficher plus [+] Moins [-]EFFECTS OF ETHANOLIC GINGER EXTRACT ON OXIDATIVE STRESS AND SOME TRACE ELEMENTS IN CADMIUM – INDUCED TOXICITY IN MALE RABBITS Texte intégral
2013
Ahlam A. AL-Rikabi Ala AL-Deen H. Jawad
This study was conducted to evaluate the antioxidant activity of ginger (zingiber officinale) in preventing oxidative stress ,lipid peroxidation and changes in trace elements induced by cadmium toxicity in male rabbits . For this purpose twenty four of adult male rabbits were divided randomly in to four groups ( 6 in each). Group 1 was given distilled water and considered as control group, group 2 received ( 10mg/kg B.w) of cadmium chloride , group 3 received ( 100mg/ kg B.w) of ginger extract plus (10mg/kg B.w) of cadmium chloride , group 4 received (200mg/ kg B.w) of ginger extract plus of cadmium chloride(10mg/kg B.w) . All treatments were orally given a single dose for 35 days. The water and food were provided add libitum. After the end of the treatment blood samples were taken for biochemical analysis to estimate serum Malondialdehyde ( MDA) , Antioxidant activity (Glutathione , Superoxide dismutase and Catalase ) and Trace elements (Zinc, Copper and Iron ) . The result showed that the administration of cadmium chloride resulted in a high concentration of malondialdehyde ( MDA) and caused a significant decrease in both superoxide dismutase and catalase and reduced glutathione. Serum levels of trace elements were also significantly decreased. While , ginger extract administration and cadmium chloride increased and restored their levels to near normal in comparing with cadmium chloride treated rabbits.
Afficher plus [+] Moins [-]DISTRIBUTION OF ECTOPARASITES INFESTED SHEEP AND GOATS IN DUHOK PROVINCE, NORTH IRAQ. Texte intégral
2013
Balqees Ahmed Ali | Ibrahim Abdulqader Naqid | Ihsan Kadir Zangana
An investigation into ectoparasites of sheep and goats was carried out in Duhok province North West region of Iraq, during six months of a year, from January to June 2010. One thousand and two hundred forty eight (1248) sheep and 954 goats in 110 flocks from 80 villages were examined. Among them overall animals (66.89%) were infested with one or more species of ectoparasites. Of 720 (57.7%) and of 753 (78.9%) sheep and goats, respectively were infested. Five different types of ectoparasites, ticks (46.7%, 34.9%), lice (3.8%, 33.8%), mites (7.1%, 0.1%), fleas (2.8%, 7.75%) and ked (1.2%, 4.5%) were identified sheep and goats, respectively. Five species of hard ticks (Ixodidae) were identified in both sheep and goats, namely Hyalomma anatolicum anatolicun (48.37%, 13.5%), H. marginatum (18.01%, 8.1%), Rhipicephalus sanguineous (21.09%, 39.93%), R. turanicus (16.8%, 49.54%) and Haemaphysalis ssp. (2.5%, 0.9%). Two species of lice were infested each one of animal these namely, Damalinia Ovis (75%) and Linognathus stenopsis (33.3%) on sheep, and D. caprae (80.74%) and L.stenopsis (19.2%) on goats. Sheep were highly infested with two species of mites, Sarcoptes scabiei (25.84%) and Psoroptes Ovis (74.15%) than the goats, which one goat was found (0.1%) infested with S. scabiei. Three species of fleas were found infested both sheep and goats. Out of 106 collected fleas (47.2%, 43.4%, 9.4%) were Ctenocephalides felis felis, Pulex irritans and Xenopsylla cheopis, respectively. However, the only one species of Ked Melophagus ovinus were infested (1.2%) of sheep and (4.5%) of goats.
Afficher plus [+] Moins [-]A COMPARATIVE STUDY OF GRASS CARP (CTENOPHARYNGODON IDELLA) WHEN CULTIVATED IN POLLUTED AND NON POLLUTED AQUATIC ENVIRONMENT Texte intégral
2013
Abdul Majeed H.Talal
Fourty fresh water grass carp fish weighing about (35.75 ± 0.60) g were obtained from fish aquaria by, Marine Science Center, University of Basrah, Iraq. First the. Fish were individually examined for external necrosis and infections. Healthy and see it very active in the study. The physiochemical characteristics of water used for acclimation in control and polluted water that were used for the fish of experiment were daily recorded. The results of the present study show that the blood parameters ( RBC and WBC counts and Hb content) for fresh water grass carp fish were greater than those values of polluted water grass carp fish and also show increased number of dead fish among polluted grass carp, in reverse of fresh water ones. Physicochemical characteristics of water did not affect the result
Afficher plus [+] Moins [-]STUDY THE EFFECT OF EXOGENOUS ESTROGEN ADMINISTRATION ON PROSTATE GLAND OF LABORATORY WHITE MICE (MUS MUSCULAUS) Texte intégral
2013
Mukhallad A. Ramadhan
A substantial advance in our understanding on the estrogen signaling occurred in the last decade. Estrogens interact with two receptors, ESR1 and ESR2, also known as ERα and ERβ, respectively. ESR1 and ESR2 belong to the nuclear receptor family of transcription factors. 24 male mice divided into four groups as following : negative control, low dose group, intermediate dose group and high dose group, the groups of treatment were injected with estrogen at different doses while the negative control group were injected with normal saline (by method of intramuscular injection for all groups) and after 6 weeks all animals had been killed and the prostate was picked up and sectioned to reveal the histopathological changes. the study found that the injection of exogenous estrogen result in marked hyperplasia of the prostatic tissue and the hyperplasia increased significantly with elevation of the estrogen dose. In conclusion injection of exogenous estrogen lead to prostate hyperplasia through its action on the estrogen receptors which present in both stromal and epithelial component of the prostate
Afficher plus [+] Moins [-]EFFECTS OF DEXAMETHASONE, ESTROGEN ADMINISTRATION ON LEPTIN, THYROID, REPRODUCTIVE HORMONE CONCENTRATION AND LIPID PROFILE OF FEMALE RABBITS SERUM. Texte intégral
2013
Rashad F.Ghadhban Ala AL-Deen H. Jawad
The aim of this study investigate the effects of long term administration of Dexamethasone (DEX.) 17 β-estradiol (EST.) on body weight, concentration of some hormones ( Leptin, thyroid and reproductive hormone) in addition to determination of lipid profile of female rabbits blood serum. Thirty local rabbits were subdivided randomly and equally into three groups. The first group was used as a control which injected with normal saline . The second group was injected daily (0.5mg /kg B.W) DEX . Intramuscularly. The third group was injected subcutaneously daily with 0.3 mg /kg B.W s/c 17 β-estradiol. All groups in this study were treated for 60 days , and blood collected every 15 days. Serum was tested for leptin, LH, FSH, T3, T4 hormones total Cholesterol(TC), HDL(height density lipo-protein), LDL (low density lipo- protein),TG (triglycerides), Body weight was taken every 15 days from the first day . The result showed significant (P≤0.05) decreased in body weight after administration of both dexamethasone (DEX.) and Estrogen (EST.), significant increased in leptin concentration with dexamethasone (DEX.) Treatment while significant decreased with Estrogen (EST.) treatment, in the other hand LH and FSH concentration showed significant decreased in both treatment dexamethasone (DEX.) and Estrogen (EST.) thyroid hormone show significant decreased with dexamethasone (DEX.) Treatment and significant increased T3 & T4 hormone with Estrogen (EST.) treatment as compare with control while the increase in the T4 it was occurred only in the periods (45and 60)days within treatment groups , lipid profile (TC,TG,LDL, HDL,VLDL) show significant increased with DEX. treatment while Significant decreased with EST. treatment
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