In the course of studying porcine enterovirus infection in piglets, the vascular endothelial cells in the CNS of colostrum-deprived piglets with polioence-phalomyelitis were investigated by electron microscope. The experimental piglets were orally infected with the porcine enterovirus serogroup 3 isolated in Korea and necropsied at 7 days postinoculation. Crystalline arrays of viral particles were found in the vascular endothelial cells of the spinal cord and cerebellum. Aggregates of immature viral particles were occasionally observed in the vascular endothelial cells in the meninges. The rough ER was deprived of ribosomes, irregularly dilated and associated with viral crystals. There were abundant cytoplasmic filaments, dilatation of perivascular space, perivascular cuffing, and the partial disruptions of endothelial cell membrane and basal lamina

The In vitro drug susceptibility of 83 strains of Bordetella bronchiseptica recoverd from Korean pigs with atrophic rhinitis was investigated by the use of disk diffusion method. The majority of the organisms were highly resistant in order of prevalence to penicillin (98.7%), ampicillin(91.5%), streptomycin (90.3%), triple sulfa (83.1%), and trimethoprim/sulfamet-hoxazole (70.7%) while none of them were resistant to gentamicin, only 3.6% to colistin, chloramphenicol and kanamycin and 6.0% to tetracycline. The percentage of the organism resistant to bicozamycin, cephalothin and neomycin were 34.9%, 34.1% and 18.4%, respectively. A high prevalence of multiple drug resistance was observed and the 3 most common resistant patterns among 35 patterns noted were Am Pc Sm Sss Sxt(26.5%), Am Cf Pc Sm Sss Sxt(12%) and Am Bm Pc Sm Sss Sxt(9.6%) patterns

Nutritional risk factors for liver damage were surveyed in Black-Pied French dairy herds using serum glutamic dehydrogenase and gamma glutamyl transferase levels to monitor liver dysfunction.The 34 herds chosen were free of parasitic liver injuries. Feeding systems, nutritional blood parameters and production characteristics are analyzed. Two risk factors appeared to be involved in hepatic injury: feeding of fresh rape (Brassica napus) and high levels of serum urea (>/=6.64 mmol/L) associated with grass pasture or dietary nonprotein nitrogen with corn silage