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Effect of central administration of indomethacin on anandamide-induced GnRH/LH secretion in the hypothalamus of anoestrous ewes
2024
Tomaszewska-Zaremba Dorota | Tomczyk Monika | Wojtulewicz Karolina | Bochenek Joanna | Pałatyńska Kinga | Herman Andrzej Przemysław
It is suggested that cannabinoids (CBs) may disturb reproduction through action on hypothalamic gonadotropin-releasing hormone (GnRH) neurons directly or indirectly through intermediates such as prostaglandins. The study aimed to determine the influence of intracerebroventricular (i.c.v.) injection of the endogenous cannabinoid anandamide (N-arachidonoylethanolamine – AEA), alone or with the prostaglandin synthesis inhibitor indomethacin (IND), on GnRH/luteinising hormone (LH) secretion. The purpose of the research was to clarify the role of endocannabinoids and their interaction with prostaglandins in the regulation of reproduction at the level of the hypothalamus and pituitary in anoestrous sheep.
Afficher plus [+] Moins [-]The influence of probiotic administration on selected leukocyte subpopulations and the serum amyloid A concentration in the peripheral blood of dairy cows during different lactation periods
2024
Brodzki Piotr | Marczuk Jan | Gorzkoś Hubert | Lisiecka Urszula | Szczubiał Marek | Brodzki Adam | Dąbrowski Roman | Krakowski Leszek | Głodkowska Katarzyna | Brodzki Nikodem
The aim of the study was to compare selected leukocyte subpopulations and the serum amyloid A (SAA) concentration in the peripheral blood of cows at different stages of lactation. The blood of cows receiving a probiotic as a dietary supplement was compared with the blood of cows not receiving it.
Afficher plus [+] Moins [-]The regulatory action of acetylcholine and its receptors on B4 and C4 leukotriene formation in the porcine endometrium after experimental inflammogenic Escherichia coli infection
2024
Jana Barbara | Całka Jarosław | Bulc Michał | Kawka Dominika
Endometritis is a very common pathology in animals which changes endometrial leukotriene (LT) formation and muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic acetylcholine (ACh) receptor (α-7 nAChR) expression patterns. With the relationship between ACh, its receptors and LT production remaining unclear, the role of M2R, M3R and α-7 nAChR in action of ACh on the 5-lipoxygenase (5-LO), LTA4 hydrolase (LTAH) and LTC4 synthase (LTCS) protein abundances in the inflamed porcine endometrium and on the tissue secretion of LTB4 and LTC4 were studied.
Afficher plus [+] Moins [-]The grey wolf (Canis lupus) as a host of Echinococcus multilocularis, E. granulosus s.l. and other helminths – a new zoonotic threat in Poland
2024
Karamon Jacek | Samorek-Pieróg Małgorzata | Bilska-Zając Ewa | Korpysa-Dzirba Weronika | Sroka Jacek | Zdybel Jolanta | Cencek Tomasz
The aim of this study was to estimate the occurrence of Echinococcus spp. and other helminth infections in grey wolves in south-eastern Poland.
Afficher plus [+] Moins [-]Fatal Sarcoptes scabiei and Demodex sp. co-infestation in wolves (Canis lupus) at the Białowieża National Park, Poland – is it a consequence of climate change?
2024
Krzysiak Michał K. | Świątalska Agnieszka | Plis-Kuprianowicz Elwira | Konieczny Andrzej | Bakier Sławomir | Tomczuk Krzysztof | Larska Magdalena
In winter 2021/2022, a wolf population in the primeval Białowieża Forest in Poland was struck by an outbreak of severe mange caused by mixed infestations of Sarcoptes and Demodex mites. We present an epidemiological analysis of this mange which caused significant morbidity and mortality.
Afficher plus [+] Moins [-]First molecular characterisation of Sarcocystis miescheriana in a pig carcass condemned during routine meat inspection in Poland
2024
Korpysa-Dzirba Weronika | Rubiola Selene | Bilska-Zając Ewa | Różycki Mirosław | Chiesa Francesco | Bełcik Aneta | Samorek-Pieróg Małgorzata | Kubacki Jakub | Łukomska Anna | Karamon Jacek | Sroka Jacek | Cencek Tomasz
This article presents the fourth detection of macroscopic cystic lesions due to sarcocystosis in domestic pigs during routine meat inspection worldwide, and the first molecular detection of Sarcocystis miescheriana in a domestic pig in Poland. Pigs can become intermediate hosts for S. miescheriana by accidental ingestion of oocysts or sporocysts present in food or water contaminated by the faeces of canids (definitive hosts).
Afficher plus [+] Moins [-]The first confirmed cases of pigeon rotavirus A (RVA) infection in domestic pigeons (Columba livia) in Poland
2024
Adamczyk, Krzysztof | Rubbenstroth, Dennis | Ledwoń, Aleksandra | Sapierzyński, Rafał | Szeleszczuk, Piotr
Although the presence of rotaviruses in pigeon samples has been reported since the 1980s, its importance as an aetiological agent of the “classical” young pigeon disease (YPD) was not proven until 2020, when the Henle–Koch postulates were confirmed for pigeon-type rotavirus A (RVA) genotype G18P(17). Material and Methods From 2011 to 2020, archived liver samples from 117 pigeons submitted by 74 individual lofts were tested for the presence of pigeon-type RVA using a VP6-specific RT-qPCR test. For four positive racing pigeons, a more detailed necropsy and histopathological analysis was performed. Results Indicators of an acute RVA infection were found in 24 out of 117 (20.5%) samples tested, the earliest in 2014. Necropsies of the four selected RVA-positive pigeons showed changes mainly in the liver, spleen and kidneys similar to those described by other researchers. The histopathological examination revealed mainly hyperaemia and necrosis in the liver, as well as mononuclear cell infiltrates in these organs. Conclusion Pigeon-type RVA is also a cause of YPD in Poland and is a serious challenge for racing pigeon breeders and veterinarians, especially during the training and flights of young pigeons.
Afficher plus [+] Moins [-]Determination of clostebol residues in the urine of slaughter animals using liquid chromatography–tandem mass spectrometry
2024
Matraszek-Żuchowska Iwona | Kłopot Alicja | Grzelak Justyna | Zdonek Paulina
Synthetic anabolic hormones, which may pose a potential risk to human health, should not be used in fattening food-producing animals. Because of the hormonal effects they cause, growth-promoting compounds are banned by legislation in the EU for use in animal husbandry. Consequently, all EU member states are required to conduct monitoring tests on the content and residues of these compounds in prescribed biological matrices to ensure the safety of food consumers. The aim of this research was to develop a liquid chromatography–tandem mass spectrometry method for the detection of the residue of one such anabolic hormone, clostebol in food animal urine.
Afficher plus [+] Moins [-]Effects of a β-glucan-enriched diet on biomarkers of oxidative stress, energy metabolism and lysosomal function in muscle tissue of European grayling (Thymallus thymallus L.)
2024
Kurhaluk Natalia | Grudniewska Joanna | Tkaczenko Halina
The grayling (Thymallus thymallus L.) has several advantages over other fish species that make it attractive for aquaculture and invest it with importance for food security. The study assessed the effects of a β-glucan-enriched diet on biomarkers of oxidative stress, energy metabolism and lysosomal function in muscle tissue of European grayling (Thymallus thymallus L.).
Afficher plus [+] Moins [-]Distribution and neurochemical characterisation of neurons containing neuregulin 1 in the enteric nervous system within the porcine small intestine
2024
Puchała Łukasz | Gonkowski Sławomir | Rytel Liliana | Wojtkiewicz Joanna | Grzegorzewski Waldemar Jarosław
The enteric nervous system (ENS) in the wall of the gastrointestinal tract is complex and comprises many neurons, which are differentiated in terms of structure, function and neurochemistry. Neuregulin 1 (NRG 1) is one of the neuronal factors synthesised in the ENS about the distribution and functions of which relatively little is known. The present study is the first description of the distribution of NRG 1 in the ENS in various segments of the porcine small intestine.
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