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EFFECT OF DIETARY LEVELS OF CALCIUM CARNITINE AND ZINC ON SEMEN CHARACTERISTICS OF CROSS-BRED CHICKEN Texte intégral
2024
R. Prabhakar | M. Moorthy | S.C. Edwin | K. Mani | V. Ramesh Sarvanakumar
An experiment was conducted to study the effect of dietary supplementation of calcium, carnitine, zinc and their combinations for 5 weeks in eighty adult crossbred cocks which were divided into eight treatment groups each consisting of ten cocks. Cocks were fed with treatment diets. The treatment groups consisted of control (T1), control + calcium 2 per cent (T2), control + carnitine 250 mg/kg (T3), control + zinc 100 mg/kg (T4), control + calcium 2 per cent + carnitine 250 mg/kg (T5), control + calcium 2 per cent + zinc 100 mg/kg (T6), control + carnitine 250 mg/kg + zinc 100 mg/kg (T7) and control + calcium 2 per cent + carnitine 250 mg/kg + zinc 100 mg/kg (T8). Semen volume revealed no significant difference between treatment groups during five weeks period. Overall sperm motility was significant (P<0.01) between treatment groups except between T4 and T5 groups. Overall sperm concentration was significantly (P<0.01) higher in all treatment groups compared to control. Inclusion of calcium, carnitine, zinc and their combinations had significantly (P<0.01) reduced the overall dead and abnormal of sperms when compared to the control group. The results revealed that inclusion of 2 per cent calcium, 250 mg/kg of carnitine, 100 mg/ kg of zinc and their combinations in feed improved the sperm motility and concentration in cross-bred cocks.
Afficher plus [+] Moins [-]IN VITRO EFFECT OF SYZYGIUM AROMATICUM ON THE MOTILITY AND ACETYLCHOLINESTERASE OF COTYLOPHORON COTYLOPHORUM Texte intégral
2024
K. Manoj Dhanraj | L. Veerakumari
Helminth parasites cause a wide variety of debilitating and frequently fatal diseases in animals. Paramphistomosis caused by the paramphistome Cotylophoron cotylophorum constitutes a major group of disease in domestic ruminants. The anthelmintic activity of Syzygium aromaticum on the motility and acetylcholinesterase (AChE) activity of the digentic trematode Cotylophoron cotylophorum was studied in vitro. The flukes were exposed to various concentrations of hexane, choloroform, ethyl acetate and ethanol extract of Syzygium aromaticum and the motility and mortality of the parasite were observed. As ethanol extract was very effective, further studies were carried out with five different sub lethal concentrations (0.005, 0.01, 0.5, 0.1 and 0.5 mg/ml) of ethanol extract of Syzygium aromaticum (SaEE). The electronic measurement of the motility of the treated parasites clearly indicate the direct impact of the drugs on the motility of the parasite. Maximum inhibition in the motility (86.27%) and AChE activity (86.86%) was observed in 0.5 mg/ml after 8h of exposure. Acetylcholinesterase (AChE) is an enzyme which is involved in neurotransmission. It is present in the cholinergic synapses in the central nervous system as well as in neuromuscular synapses where it rapidly hydrolyzes acetylcholine. Inhibition of AChE in the parasite results in muscular paralysis and the parasite lose its biochemical hold fast and get expelled from the host.
Afficher plus [+] Moins [-]TOXICOPATHOLOGICAL STUDIES OF DI BUTYL PHTHALATE IN MALE WISTAR RATS Texte intégral
2024
Rina R. Patel | B. P. Joshi | D.J. Ghodasara | J. H. Khorajiya | Priya D. Ghodasara | Sunanda Pandey
Di butyl phthalate (DBP) is a most important plasticizer which is suspected as reproductive toxicant and exposure to it has the potential effect on the human as well as animal reproductive system. The research work was conducted to evaluate the repeated dose toxicity of di butyl phthalate in male wistar rats. The animals were divided in four different groups with 12 male rats in each group. Group I served as control and was administered corn oil (2 ml/kg body wt.) while groups II, III, and IV were administered DBP orally at the dose rate of 500, 1000 and 2000 mg/kg respectively for consecutive seven days. Three rats from each group were sacrificed at 24 hrs, 7th day, 14th day and 21st day after initial dosing. There was gradual decrease in WBC count, RBC count, Hb, HCT, MCV, MCH, MCHC, gradual increase in albumin, gradual decrease in total protein, glucose values, gradual decrease in relative weights of the testes, epididymis, prostate and seminal vesicles as well as increase in liver weight with increase in dose of DBP in group II, III and IV as compared to control group at different intervals of sacrifice. Grossly reductions in the size of testes were observed only in rats that belonged to high dose group IV on 21st day. The plasticizer DBP was found to produce histopathological lesions in male reproductive organs as well as in liver in dose dependent manner after oral administration. The finding suggested that DBP can cause toxicity lesions in male reproductive organs especially in testes and epididymis at the dose rate of 500 to 2000 mg/kg body weight in wistar rats.
Afficher plus [+] Moins [-]ATTITUDINAL AND MOTIVATIONAL TRAITS ON COMMUNICATIONAL BEHAVIOUR OF TRIBAL AND NON-TRIBAL DAIRY FARMERS Texte intégral
2024
L. Sanathoi Khuman | P. Hazarika | K.K. Saharia | Tapan Kr. Amonge | Monosri Johari
An investigation among fifty each tribal and non-tribal dairy farmers in four dairy dominated development blocks of Cachar district of Assam revealed that, among tribal and non-tribal farmers attitude towards dairy farming, economic motivation and source of motivation for dairy communication were found to have favourable scores with positive and significant correlation with communication behaviour. Regression analysis revealed that source of motivation for dairy communication crucially influenced communication behaviour of dairy farmers. Further, there louid significant difference between the tribal and non-tribal dairy farmers indicating sensitive strategies for dairy development in the area involving the veterinarians.
Afficher plus [+] Moins [-]EMPLOYMENT AND INCOME OF NON - MECHANIZED FISHERMEN IN THE COASTAL FISHING VILLAGES OF PUDUKKOTTAI DISTRICT , TAMIL NADU Texte intégral
2024
K. Maheswaran | M. Rajakumar | R. Senthiladeban | T. Umamaheswari
This study on the employment and income of fishermen in the nonmechanized fishing craft categories of Pudukkottai District, Tamil Nadu was carried out with the specific objectives of estimation of costs per fishing trip, seasonal employment and identification of the factors determining quantity of fish catch. The sample size was 90 fishermen respondents equally distributed among canoe, vallam and FRP boat operating villages. Descriptive analyses were used to estimate the items of costs and returns, pattern of employment and levels of net income per fishing trip for different fishing seasons. The average annual fishing days of employment was 243, 138 and 136 for canoe, vallam and FRP boat categories, respectively. The overall mean gross income per fishing trip was r. 1843.42, r.2444.77 and r. 2116.97 for canoe, vallam and FRP boat categories, respectively.
Afficher plus [+] Moins [-]CLINICAL, HAEMATO - BIOCHEMICAL, RADIOLOGICAL AND HISTOPATHOLOGICAL STUDIES OF HORN AFFECTIONS IN BUFFALOES Texte intégral
2024
K. Rama Rao | Makkena Sreenu | K.B.P. Raghavender | P .V. S. Kishore
A study was conducted in buffaloes suffering with horn affections .The physiological and haematological parameters were within the normal limits in all the horn affections, but the plasma cortisol and C-reactive protein were more than the normal levels in all the horn affections for initial periods. A radiological sign like increased radio-opacity was noticed in the septic horn cases. Microscopic examination of biopsy samples of horn and skin following amputation revealed normal histology except in horn cancer where squamous cell nests and mitotic figures in the squamous cells were observed.
Afficher plus [+] Moins [-]RING WORM INFECTION IN A GOAT - A CLINICAL CASE REPORT Texte intégral
2024
G. Kalaiselvi | N. Daniel Joy Chandran | M. Vidhya
A 3 months old goat kid presented to Veterinary Dispensary, Chetiyur, Salem district Tamil Nadu with case history of ring like patches on the body complete alopecia, frequent itching with thick and scaly skin . Dermatophytes are cited among the most frequent causes of dermatological problems in domestic animals. The superficial mycoses caused by dermatophytes are called dermatophytosis- Trichophyton are usually the cause of dermatophytosis in domestic animals. Trichophyton verrucosum is the major fungal agent encountered in case of goats. Direct microscopic examination of skin scrapping with 20% KOH revealed long chains densely packed chlamydiospore found with swollen hyphae. The skin scrapping materials cultured in thiamine inositol nutrient supplement selective culture medium. Long chains of densely compacted chlamydospores present in lactophenol cotton blue staining. The goat kid recovered within a week by external antifungal and systemic antibiotic and nutritional therapy with good hygienic management.
Afficher plus [+] Moins [-]PARACETAMOL POISONING IN A CAT AND ITS TREATMENT Texte intégral
2024
P. Pothiappan | T. Muthuramalingam | R. Sureshkumar | G. Selvakumar | M. Thangapandiyan | G. D. Rao
A two year old male cat was presented to the Emergency and Critical Care Unit with a history of inappetence after paracetamol 300 mg (Half tablet of 600 mg) administration overnight. On clinical examination the cat had a mild elevation of vital signs. Based on history and clinical signs the case was diagnosed as paracetamol poisoning and treated with activated charcoal a total dose of 3 g)orally and N-acetyl cysteine intravenously at 140 mg/kg along with Ringer’s lactate, 10 ml/kg b.wt. The owner was advised to administer Nacetyl cysteine at 70 mg/kg orally for every 6 hours for five times. After treatment the cat was recovered successfully.
Afficher plus [+] Moins [-]EVALUATION OF FEED GRADE ENZYMES BY INVITRO METHOD USING DE OILED RICE BRAN (DORB) AS SUBSTRATE Texte intégral
2024
R. Kavitha | D. Chandrasekaren
Commercial cocktail enzymes were collected from the market and evaluated for enzyme activity. Among the enzyme preparations which is having more enzyme activity was selected for invitro evaluation by using de oiled rice bran as substrate. The results of the present study indicated that a significant (P < 0.05) and linear increase in the release of glucose and reducing sugars with the increase in the level of enzyme mixtures from 0 to 7.5 mg / 10 g of DORB.
Afficher plus [+] Moins [-]CLONING AND EXPRESSION OF ENDOGLUCANASE GENE FROM TERMITE GUT BACTERIA FOR IMPROVING FIBRE DIGESTION * Texte intégral
2024
N.V. Aruna | K.Vijayarani
The present study describes the cloning and expression of endoglucanase gene from termite gut bacteria. Salmonella enterica with cellulolytic activity was isolated from the gut of the termite Odontotermes formosanus and characterized by sugar fermentation tests, biochemical tests and 16S rRNA sequencing. Endoglucanase gene was amplified from the genomic DNA of the bacteria and the purified 1.72 Kbp Polymerase chain reaction product was cloned into pET100 prokaryotic vector. The recombinant plasmid expressed a 65kDa endoglucanase which reacted with anti His-HRP antibody in western blotting. Cellulolytic activity of the recombinant plasmid was confirmed by congo red assay and the enzyme activity was found to be 54 Units/ml.
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