Obesity is a metabolic condition that causes people to develop a variety of diseases and has emerged as a serious global public-health problem. Tocotrienol, a member of the vitamin E family that comes from the annatto bean (Bixa orellana), is special in that it doesn't contain alpha-tocopherol and instead mostly consists of delta-tocotrienol (approximately 90%) and gamma-tocotrienol (about 10%). This investigation aimed to ascertain whether annatto tocotrienol could improve certain biochemical indicators and metabolic hormones in male rats fed a high-fat diet. Eighteen adult male rats in total were split into three groups randomly (6 for each). Control group was given a diet low in fat (LF 10 % kcal from fat), High fat diet (HFD) group was fed with high fat diet (HF 60 % kcal from fat), And high fat diet with tocotrienol (HFDT) group was fed with high fat diet additive tocotrienol (60 mg/kg) dissolved in olive oil (1ml/kg) for 12 weeks. Tocotrienol treatment led to a significant decrease in total protein and globulin compared with the high-fat diet group and it significantly increased HDL-C compared with rats fed on a high-fat diet and control groups. While, tocotrienols significantly reduced the level of LDL and insulin hormone in the High fat diet plus tocotrienols group compared to the other groups.

The gastrointestinal tract provides the biological environment for nutrient digestion and absorption. Its physical and chemical barriers are crucial to protect from invading pathogens and toxic substances. On this basis, the intactness of the gastrointestinal tract, with its multiple functions and impacts, is one of the key prerequisites for human and animal health. Undoubtedly, the functions of a healthy gut system also largely benefit the welfare and performance of animals in farming systems such as poultry industries. Broiler chickens grow rapidly, as a result of rigorous genetic programs, due to the high absorption capacity of intestinal epithelia for nutrients, the quick transport of nutrients to the muscle, and their efficient conversion into energy and biomass. Due to oxygen metabolism or enteric commensal bacteria, intestinal epithelial cells create reactive oxygen and nitrogen species physiologically. However, increased generation of these oxidants goes along with the formation of free radicals resulting in oxidative stress causing lipid peroxidation and dramatic molecular changes in the structure and function of the cell and mitochondrial membranes. These effects contribute to chronic oxidative stress and inflammation of the gastrointestinal tract and generally affect all chicken organs, tissues, and cells. Hence, all forms of chronic stress, regardless of the origin, negatively impact the chicken's overall performance, health, and welfare. This review article highlights some enteric inflammation models and biomarkers to evaluate gut integrity in chickens and discusses the repercussions that chronic stress and intestinal inflammation have on the health and performance of commercial poultry.

The presence of microorganisms is one of the major factors affecting the quality of smoked fish sold in the open markets. Smoked blue whiting fish (Micromesistius poutasou), commonly called (Panla) sold in the Odeomu market in Osun State, were analyzed for microbial contaminants. Isolates were identified using conventional biochemical methods, and antibiotics susceptibility testing was carried out using the disc diffusion method. The total bacterial counts (TBC) results showed that the fish samples had high bacterial counts, ranging from 2.1×103 to 9.2×103 colony-forming units (CFU)/g. Bacteria isolated from the fish samples were: E. coli (45.46%), Enterobacter spp. (1.01%), Klebsiella spp. (6.06%), Proteus spp. (9.09%), Salmonella spp. (7.07%), Shigella spp. (19.19%), Bacillus spp. (4.04%) and Staphylococcus spp. (8.08%). The antibiotic sensitivity pattern of Gram-Negative bacteria indicated that all the isolates were resistant to more than three antibiotics. All E. coli isolates were resistant to augmentin and ceftazidime, 82.2% were resistant to cefuroxime, 17.7% to gentamicin, and 6.7% to ofloxacin. Screening of resistance genes showed that all six selected multiple antibiotic-resistant E. coli isolates tested harbored TEM gene, and two isolates (33.33%) harbored the aac (3)-II gene. None of the isolates harbored SHV, CTX-M, and qnr B genes. Our results showed that smoked blue whiting fish may pose a significant risk of spreading antibiotic-resistant bacteria that contain multiple antibiotic-resistance genes, highlighting a serious public health concern

In order to evaluate the infection of veterinarians by brucellosis in Algeria and to study the associated epidemiological factors, we created a survey consisting of 21 questions that was distributed in paper and digital versions. We collected responses of 100 veterinarians. The survey revealed that 15% of the veterinarians got infected with brucellosis during their practice. Almost half (47%) contracted the disease through direct contact with diseased animals and/or their products, mostly during intervention for retained placenta (75%); 20% became infected during vaccination campaigns against brucellosis, due to unprotected hands, where13% were infected through consumption of raw milk. Factors such as the frequency of encountering brucellosis farms, negligence in wearing protective equipment, lack of training in handling the vaccine, as well as lack of work hygiene were reported by these professionals.

Infectious bronchitis (IB) is a viral disease that causes serious economic losses in the broiler industry. This study evaluated the effectiveness of Olea europaea leaves and propolis extracts (OLP) mixture at a rate of 400 μg and 100 mg/mL, respectively, in curing IB in broiler chickens. One-day-old Ross broiler chicks were randomized into four groups (G) of twenty-one chicks; G1 (control negative; no infection and treatment); G2 (no infection, treatment only), G3 (control positive; infection only and no treatment) and G4 (infection and treatment) that infected with IBv (106 EID50/ml) at 21 days old. The OLP treatment was applied for birds in G2 and G4 at a dose of 0.5 mL/liter drinking water for three successive days. The growth performance, clinical and pathological examinations, and viral shedding were evaluated. The use of the OLP resulted in protection from IB infection through the significant improvement of performance parameters such as weight gain and feed conversion ratio, decrease in mortality rate, lowering disease severity, and rapid recovery from the observed clinical signs (mainly respiratory signs), gross and microscopic lesions in the trachea, lung, and kidneys as compared to those in the positive control (G3). Moreover, the viral shedding in the OLP-treated chicks (G4) was significantly decreased in tracheal and cloacal swabs to a rate less than 3×103 IBv genome copy number and became not detectable at 14-days post-infection (dpi) in their cloacal swabs. In conclusion, OLP can potentially display an antiviral effect against IB in broiler chickens. Therefore, adding OLP to the chicken drinking water is recommended to prevent and control IB [Ger. J. Vet. Res. 2023; 3(2.000): 1-10]

Bovine brucellosis is an infectious zoonotic disease of great impact on animal welfare and has significant economic implications on livestock farm worldwide. The disease is caused primarily by Brucella abortus (B. abortus), while B. melitensis is less common, and B. suis infection is rare. B. melitensis is the most common causative agent of brucellosis in small ruminants and humans. Although the main host of B. melitensis is considered to be small ruminants, this bacterium is also present in large ruminants. Despite brucellosis has been eradicated in many European countries, it is still endemic in Mediterranean countries and Turkey. The most prevalent Brucella species in the Mediterranean basin and Turkey is B. melitensis biovar (bv) 3. Previous studies have reported that B. melitensis bv2 is quite low in Turkey. This is the first study to isolate B. melitensis bv2 from cattle in Turkey. The strains were characterized using classical biotyping methods and then were molecularly confirmed. Multilocus variable number tandem repeat analysis (MLVA-16) typing of the strains revealed a novel genotype (1-5-3-13-3-2-3-2-4-41-8-5-4-3-3-7), which matches the Multilocus sequence typing (MLST) profiles in the database of ST8 (3-2-3-2-1-5-3-2-8). These results indicate that B. melitensis bv2 can easily infect cattle and this has to be considered in the epidemiology and control of bovine brucellosis. Circulating the highly pathogenic B. melitensis bv2 in cattle farms is of public health concern. [Ger. J. Vet. Res. 2023; 3(2.000): 11-15]

In this study, a Brucella antigen-capture ELISA (Ag-cELISA) prototype was developed. To study the validity of the developed Ag-cELISA, milk samples collected from Brucella-positive goats (n=120) and cattle (n= 64), as well as from unknown Brucella-status cattle (n=105) and sheep (n=65) herds were tested by Ag-cELISA, I-ELISA, and culture method. All Brucella-positive samples were confirmed using PCR. It was found that the developed Ag-cELISA could detect 50-100 bacteria per well (equivalent to 103 to 2×103 cells per mL) as the lowest limit of detection (LOD) and was therefore considered moderately sensitive to detect brucellae in milk. In an infected goat herd, out of 120 milk samples, 41, 32, and 17 were positive by Ag-cELISA, I-ELISA, and culture, respectively. Ag-cELISA detected 15 positive cases out of 17 culture-positive milk samples. Two culture-positive milk samples were not detected in Ag-cELISA. The relative sensitivity and specificity between Ag-cELISA and I-ELISA were 78% and 100%, respectively. In an infected cow herd, out of 64 milk samples, 32, 23, and 11 were found positive by Ag-cELISA, I-ELISA, and culture, respectively. Ten out of 11 culturally positive milk samples were found positive by Ag-cELISA. The relative sensitivity and specificity between the Ag-cELISA and I-ELISA were 71.9% and 100%, respectively. From randomly collected 105 cow and 110 sheep milk samples from herds of unknown Brucella-infection status, three (2.85%) and five (4.5%) samples were found positive using Ag-cELISA, respectively. These results showed that Ag-cELISA could be used to detect brucellae in milk more practically and safely than bacterial culture. On the other hand, this information re-affirms that milk can be an important source of brucellosis and creates a public health risk in humans; therefore, increased public awareness is of utmost importance

The efficiency of somatic cell nuclear transfer (NT) in pigs is low and requires enhancement. We identified the most efficient method for zona pellucida (ZP) removal and blastomere aggregation in pigs and investigated whether the aggregation of NT and parthenogenetic activation (PA) of blastomeres could reduce embryonic apoptosis and improve the quality of NT-derived embryos by investigating. Embryonic developmental competence after ZP removal using acid Tyrode’s solution or protease (pronase E). The embryonic developmental potential of NT-derived blastomeres was also investigated using well-of-the-well or phytohemagglutinin-L. We analyzed apoptosis in aggregate-derived blastocysts. The aggregation rate of protease-treated embryos was lower than that of Tyrode’s solution-treated embryos (69.2% vs. 88.3%). No significant difference was observed between phytohemagglutinin-L and well-of-the-well (35.7%–38.5%). However, 2P1N showed a higher number of blastocysts compared to 3N (73.8% vs. 24.3%) and an increased blastocyst diameter compared to the control and 1P2N (274 μm vs. 230–234 μm). In blastomeres aggregated using phytohemagglutinin-L, the apoptotic cell ratio was significantly higher in 1P2N and 3N than in 3P (5.91%–6.46% vs. 2.94%, respectively). Our results indicate that aggregation of one NT embryo with two PA embryos improved the rate of blastocysts with increased blastocyst diameter.

Feline panleukopenia virus (FPV), feline calicivirus (FCV), and feline herpesvirus type-1 (FHV-1) are major infectious pathogens in cats. We evaluated the immunogenicity of a new vaccine containing inactivated FPV, two FCVs, and FHV-1 in animals. An FPV, two FCVs, and an FHV-1 isolate were continuously passaged 70, 50, 80, and 100 times in CRFK cells. FP70, FC50, FC80, and FH100 were propagated and used as vaccine antigens. Two inactivated feline virus vaccines, feline rehydragel-adjuvanted vaccine (FRAV) and feline cabopol-adjuvanted vaccine (FCAV) were prepared and inoculated into mice and guinea pigs. Humoral immune responses were measured using hemagglutination inhibition (HI) for FPV and virus-neutralizing antibody (VNA) for two FCVs and FHV-1 tests. Serial passages in CRFK cells resulted in increase in titers of FPV and two FCVs but not FHV-1 The FCAV induced higher mean HI and VNA titers than the FRAV in guinea pigs; therefore, the FCAV was selected. Cats inoculated with FCAV developed a mean HI titer of 259.9 against FPV, and VNA titers of 64, 256, and 3.2 against FCV17D03, FCV17D283, and FHV191071, respectively. Therefore, cats inoculated with the FCAV showed a considerable immune response after receiving a booster vaccination.

Rapid immunochromatography test (RICT) kits are commonly used for the diagnosis of canine parvovirus (CPV) because of their rapid turnaround time, simplicity, and ease of use. However, the potential for cross-reactivity and low sensitivity can yield false-positive or false-negative results. There are 4 genotypes of CPV. Therefore, evaluating the performance and reliability of RICT kits for CPV detection is essential to ensure accurate diagnosis for appropriate treatment. In this study, we evaluated the performance of commercial RICT kits in the diagnosis of all CPV genotypes. The cross-reactivity of 6 commercial RICT kits was evaluated using 8 dog-related viruses and 4 bacterial strains. The limit of detection (LOD) was measured for the 4 genotypes of CPV and feline panleukopenia virus. The tested kits showed no cross-reactivity with the 8 dog-related viruses or 4 bacteria. Most RICT kits showed strong positive results for CPV-2 variants (CPV-2a, CPV-2b, and CPV2c). However, the 2 kits produced negative results for CPV-2 or CPV-2b at a titer of 105 FAID50/mL, which may result in inaccurate diagnoses. Therefore, some kits need to improve their LOD by increasing their binding efficiency to detect all CPV genotypes.