Objective: This study aimed to explore the incidence and antimicrobial resistance (AMR) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus in raw milk and some Egyptian dairy products, namely Kariesh cheese and rice with milk. Material and Methods: 112 samples (70 raw milk, 30 Kariesh cheese, and 12 rice with milk) were randomly collected from different districts in Cairo and Giza, Egypt. The samples were examined for E. coli, S. aureus, and B. cereus presence. The susceptibility of the obtained isolates was tested against 11 antimicrobials using the disk diffusion method, and further, the presence of AMR genes was examined. Results: The incidences of E. coli, S. aureus, and B. cereus were 69.64%, 12.5%, and 16.7% in the examined samples, respectively. The antibiogram indicated that E. coli isolates (n = 60) were resistant to gentamycin (73.33%), ampicillin (AM, 53.3%), and cefotaxime (CTX, 16.66%). Multidrug-resistant (MDR) E. coli strains (n = 5) were tested for β-lactams resistance genes. blaTEM was detected in all isolates, and two of them additionally carried blaCTX-M. Staphylococcus aureus isolates (n = 10) were resistant to AM (100%), followed by tetracycline (TE), CTX, and gentamycin (60% each). All MDR S. aureus strains (n = 4) carried blaZ and tetK, and three of them additionally carried aac(6')-aph (2''). Bacillus cereus isolates (n = 30) showed resistance to AM (100%), amoxi¬cillin (20%), and TE (6.66%). bla and tetA genes were detected in all MDR B. cereus isolates (n = 6). Conclusion: Our findings denote the high incidence of potential health hazards in raw milk and some of its products and the existence of AMR bacteria, including MDR strains, which can cause human illnesses that are difficult to treat. [J Adv Vet Anim Res 2023; 10(2.000): 275-283]

Objective: This research was designed to assess the influence of the administration of a lithogenic diet, hydrogen peroxide, and vitamin AD3E on rabbits' gallstone formation and to envisage the expression of osteopontin (OPN) in their hepatic tissues. Materials and Methods: Twenty-four healthy local mature rabbits of both genders were divided into four equal groups. At the end of the feeding period, samples of blood were taken from all rab¬bits after they had fasted overnight to estimate the serum lipid profile. And some of the hepatic tissue has been preserved at −28°C for molecular analysis and gene expression. Results: The gallstones were formed 100% in the GIII and 50% in the GIV. The mRNA OPN expression showed a significant increase in the GIII when compared with other groups. In Groups III and II, the serum levels of total cholesterol, Triglyceride, L-C, low-density lipoprotein-choles, and VLDL-C were significantly increased when compared with GI, while in GIII, the serum level of high-density lipoprotein-cholesterol was significantly decreased when compared with GI. Conclusion: It was concluded that the expression of the mRNA OPN was increased in the hepatic tissue of gallstone-formed rabbits. [J Adv Vet Anim Res 2023; 10(2.000): 301-307]

Objective: The feeding effects of DoLa (a combination of Dayak onion extract and probiotic Lactobacillus acidophilus) on hematological indices and lymphoid organs as indicators of broiler health status were evaluated in the present study. Materials and Methods: 192 1-day-old unsexed broilers of the CP 707 strain with a body weight of 46.43 ± 1.65 gm were randomly divided into 4 dietary treatments with 6 replications. The dietary treatments applied were basal diet (BD) as a control with a code of DoLa0, BD + 0.1% DoLa (DoLa1), BD + 0.2% DoLa (DoLa2), and BD + 0.3% DoLa (DoLa3). The parameters monitored included hemoglobin (Hb), red blood cell (RBC), heterophile (H), lymphocyte (L), white blood cell (WBC), heterophile-lymphocyte (H/L) ratio, the lymphoid organs (bursa Fabricius, spleen, and thy¬mus) relative weight, as well as carcass weight. Results: The results indicated a significant improvement in WBC, L, and carcass weight (p < 0.05) as the feeding level of DoLa increased while the H and H/L ratio decreased. However, the dietary inclusion of DoLa did not affect the lymphoid organs' relative weight, RBC, and Hb concentrations. Conclusion: The mixture at 0.3% significantly improved health status through the indicators of hematological indices, lymphoid organs, and carcass weight of broilers. [J Adv Vet Anim Res 2023; 10(2.000): 269-274]

Objectives: Recent clinical studies suggest that oxidative stress is one of the key players in the pathogenesis of coronavirus disease 2019 (COVID-19), and N-acetylcysteine (NAC), a potent anti¬oxidant, has been shown to improve clinical outcomes in COVID-19 patients. We conducted a systematic review and meta-analysis of the literature published on the therapeutic intervention of NAC on COVID-19 infection. Methods: We searched PubMed, Google Scholar, and Science Direct. We identified and screened eight studies with 20,503 participants, including 2,852 in the NAC-treated group and 17,651 in the placebo group, which reported the effect of NAC on COVID-19 infection. A meta-analysis was performed using forest plots under fixed effect estimates based on the standardized mean difference (SMD) and risk ratio (RR). Results: Pooled analysis showed that NAC was associated with lower mortality in patients with COVID-19 compared with the placebo group [RR, 0.65; (95% CI: 0.56 to 0.75); p < 0.0001]. Similarly, C-reactive protein (CRP) [SMD, −0.32; (95% CI: −56 to −0.09); p = 0.0070] and D-dimer [SMD, −0.35, (95% CI: −0.59 to −0.10; p = 0.0062] levels were significantly decreased, and the oxygenation marker, PaO2/FiO2 ratio, was increased in the NAC-treated group compared with the placebo group [SMD, 0.76; (95% CI: 0.48 to 1.03); p < 0.0001]. Conclusion: Although the number of included studies was minimal, this meta-analysis suggests that NAC may have a positive effect on COVID-19 outcomes, specifically, a significant decrease in CRP and D-dimer levels and a significant increase in oxygen saturation, which decreased mortality. We have also presented a comprehensive review of the role and mechanisms of NAC in patients with COVID-19. [J Adv Vet Anim Res 2023; 10(2.000): 157-168]

Objectives: The aim of the study was to assess the feasibility of fodder Sorghum as poultry feed in terms of growth performance (plant height and fresh weight), nutritional quality (moisture, ash, crude protein, extract ether, crude fiber, extract material without nitrogen, and metabolic energy), and scanning electron microscopy energy dispersive X-ray (SEM-EDX). Materials and Methods: The study used a completely randomized design with six treatments and three replications. The treatments consisted of planting times of 24, 48, 72, 120, and 240 h and a control (0 h). Results: The results showed that there was a significant effect (p ≤ 0.05) when planting Sorghum fodder (SGF) on growth performance and moisture, but it had no significant effect on fresh weight, ash, crude protein, extract ether, crude fiber, nitrogen-free extract, energy metabolic aspects, and SEM-EDX. Conclusion: SGF is suitable as a feed ingredient for poultry in terms of nutrition and contains ZrO2, which functions as an antifungal. [J Adv Vet Anim Res 2023; 10(2.000): 222-227]

Objective: In this work, the effect of probiotics on the state of the microbial background of the livestock building, on the state of udder teats, and on the prevalence of latent mastitis was investi¬gated. Long-term use of the consortium has bactericidal activity against all cultures studied, especially the causative agent of mastitis, Staphylococcus aureus, except Proteus mirabilis, Proteus vulgaris, and Escherichia coli. Materials and Methods: The washes from animal housings and milk samples were collected from the dairy farms "Astana-Onim" Joint Stock Company and "Rodina" Limited Liability Partnership (Kazakhstan). The cleaning solutions and probiotic agents were applied directly to the udder teats of cows before and after milking. Diagnosis of subclinical mastitis was performed using the Kenotest rapid mastitis test. Directly counting the number of somatic cells in the collected milk samples from each cow was performed on a somatic cell counter. Results: Pathogenic microorganisms, including S. aureus bacteria (50% of samples) and bacteria of the E. coli group, Enterobacter aerogenes, and P. mirabilis (36% of samples), were detected on the udder skin and milk wipes. Using a consortium of probiotic microorganisms positively affects the mammary gland more quickly than using mastitis prevention agents alone. Probiotic use for a month resulted in a significant improvement in udder teat condition, with 60.7% of teats showing normal physiological reaction to milking, a decrease in complicated hyperkeratosis, and an increase in uncomplicated mastitis. The studies showed that in the experimental group, there was a 1.5-fold reduction in the number of cows with clinically pronounced mastitis. The experimental group showed no significant changes in the number of animals with high somatic cell levels before and after the study, while the control group without probiotics had a significant increase in dis¬eased animals after 1 month. Conclusion: The use of a probiotic consortium has shown promising results in reducing the inci¬dence of mastitis and improving milk quality in cows. [J Adv Vet Anim Res 2023; 10(2.000): 185-195]

Objective: This study analyzes the mycobiome in wild and captive Sumatran orangutans. Materials and Methods: Nine orangutan feces samples from the wild and nine from captivity were divided into three repeats from 11- to 15-year-olds in good health. The Illumina platform for analysis of ITS bioinformatics was used according to the Qiime2 and CCMetagen approaches. Results: Wild Sumatran orangutans include 53% Ascomycota, 38% uncultured fungi, and 4% Basidiomycota. Orangutans in captivity are 57% Ascomycota, 26% uncultured fungi, and 2% Basidiomycota. Based on genus level, uncultured Neurospora (31%), Penicillium (10%), Aspergillus (3%), Fusarium (3%), Candida (2%), Cutaneotrichosporon (2%), and Limonomyces (2%) are found in wild orangutans. The most prevalent genus among captivity orangutans is Aspergillus (32%), followed by fungal sp. (11%), Lasiodiplodia (18%), Devriesia (2%), and Sordariomycetes (2%). According to the Chao1 diversity index and Shannon and Simpson, there was no significant difference between wild and captive Sumatran orangutans. Conclusion: Neurospora is unique to wild Sumatran orangutans, although Aspergillus predom¬inates in captive orangutans. We hypothesize that the gut mycobiome of wild orangutans will resemble that of orangutans in captivity. The excellent range of food sources in the forest does not result in the prevalence of fungi in the typical gut microbiome. [J Adv Vet Anim Res 2023; 10(2.000): 228-236]

Objectives: Ducks suffer a huge economic loss as a result of infections with Pasteurella multocida and Riemerella anatipestifer, which cause high morbidity and mortality. Because these patho¬gens induce similar clinical symptoms when coinfections occur, it is very difficult to differentiate between them based just on clinical signs. Hence, these major pathogens must be quickly and accurately detected. Materials and Methods: A total of 104 birds ranging from 2 days to 4 weeks old were collected from Egyptian farms, and the outcomes were compared statistically. Conventional cultural iden¬tification procedures and a direct multiplex polymerase chain reaction assay were utilized to recognize both pathogens in a single tube reaction simultaneously. Then, the obtained isolates were characterized phenotypically and genotypically. Results: Clinical signs appear at 2–4 weeks of age with respiratory distress (dyspnea), white fluid feces, and stunting. The scrutinized data demonstrated a significantly higher detection rate by PCR directly compared to classical culture procedures. Pasteurella multocida was detected only by PCR. The disc diffusion technique against ten antibiotics showed absolute susceptibilities to amik¬acin, doxycycline, and florfenicol. High levels of beta-lactam resistance were observed. Riemerella anatipestifer isolates were screened for pathogenicity and plasmid-borne blaTEM genes. All six isolates harbored five virulence genes: aspC, RA46, m28, pstS, and Nlp/P60. Moreover, blaTEM was identified into four isolates and deposited to GenBank with accession numbers OP347083, OP347084, OP347085, and OP347086. Conclusion: These results suggest advanced PCR assays can be applied to the field for rapid and valuable diagnosis of two significant pathogens and focus on the worth of ducks in the propaga¬tion of transferable antibiotic resistance genes into the environment. [J Adv Vet Anim Res 2023; 10(2.000): 211-221]

Objective: Whole genome sequencing (WGS) of Aeromonas veronii Alim_AV_1000 isolated from ulcerative lesions of Shing fish (stringing catfish; Heteropneustes fossilis) was performed during the outbreak year 2021. Materials and Methods: Using next-generation sequencing (Illumina) technology, WGS was accomplished, resulting in the sequencing, assembly, and analysis of the entire genome of the A. veronii strain. Moreover, the genomic features, virulence factors, antimicrobial resistome, and phylogenetic analysis for the molecular evolution of this strain were also examined. Results: The genome size of the A. veronii Alim_AV_1000 strain was 4,494,515 bp, with an average G+C content of 58.87%. Annotation revealed the known transporters and genes linked to virulence, drug targets, and antimicrobial resistance. Conclusion: The findings of the phylogenetic analysis revealed that the strain of the present study has a close relationship with the China strain TH0426 and strain B56. This study provides novel information on A. veronii isolated from Shing fish in Bangladesh.

Objective: The escalating prevalence of canine oral tumors has emerged as a considerable health concern. This study examined the prevalence, types, and distributions of lesions linked to canine oral tumors. Material and Methods: The medical records of 526 dogs diagnosed with oral tumors were analyzed to determine the prevalence, types, and distributions. Tumor stages were classified into four categories using the tumor node metastasis system. Results: Among the 526 dogs, there were 118 cases of benign tumors and 408 cases of malignant tumors. Acanthomatous ameloblastoma was the most common benign tumor (43.22%), while melanoma was the most common malignant tumor (51.23%). The gingiva was the most common site for both benign and malignant lesions, accounting for 89.83% and 63.73% of cases, respectively. Melanoma, squamous cell carcinoma, and fibrosarcoma were primarily located in the gin¬giva, whereas osteosarcoma was commonly found in the mandible. Most tumors were classified as stage III (ranging from 46.84% to 74.58%). Of the reported cases, 56.08% were males and 43.92% were females, and the most common breed was mixed at 30.41%, followed by Poodle at 14.25% and Shih Tzu at 11.40%. Moreover, patients with malignant oral tumors (11.6 ± 3.1 years) were significantly older than those with benign tumors (8.9 ± 3.4 years, p < 0.0001). Conclusion: Gingiva was the primary site for oral tumors, and mainly classified as stage III. These findings emphasize the increasing occurrence of oral tumors in senior and geriatric dogs and provide insights into the prevalent types and distribution.