Studying of cultural, morphological and enzymatic properties of isolated from a calf strain Salmonella enteritidis was realized in the conditions of the Republic of Belarus. There was presented quantity and results of bacteriologic examinations for cattle salmonellosis realized in the district veterinary services in Belarus in course of three years. There was presented quantity and results of serovars of salmonellas isolated from the pathologic material from cattle obtained in course of three-year analysis: S. typhimurium, S. enteritidis, S. dublin, S. panama. The following enzicymatic properties of the studied strain were analyzed: glucose, sucrose, manna sugar, citrate, methyl red, Voges-Proskauer reaction, mobility, indole, sulphuretted hydrogen, and urea. Determination of antibiotic susceptibility of strain S. enteritidis was presented. Initial data for calculation LD50 was given. Research results showed that the studied microorganisms were the representatives of family Enterobakteriacea, genus Salmonella, species S. enteritidis

Results of studies of acute and chronic toxicity of control samples of long acting boluses on the basis of fenbendazole for the treatment of strongylatosis (Strongylata) of gastro-intestinal tract of cattle realized in the conditions of the Republic of Belarus were presented. White rats and mice were used for studying of acute and chronic toxicity of the prolonged anthelmintic. The preparation was tested in doses of 400; 800; 1200; 1600; and 2000 mg/kg of body weight. Prolonged anthelmintic was administrated introgastrally in the form of suspension. Preparation was reduced to fragments before application. White rats with body weight of 80-100 g were divided in three groups and were used for testing. Bolus of prolonged activity was reduced to fragments and mixed with feed before application. Rats ate the preparation during 30 day in dozes of 250 mg/kg, 375 mg/kg, and 500 mg/kg. Study of acute toxicity of preparation established that LD0 for white mice was 400 mg/kg; LD16 - 620 mg/kg; LD50 - 1080 (1062,7 / 1097, 3) mg/kg; LD84 - 1660, LD100 - 200 mg/kg. Signs of animal toxicosis were revealed in shakiness, depression, whiteness of visually accessible mucous coat, cramps. Catarrhal-haemorrhagic gastroenteritis and numerous effusions of blood on mucous coat of digestive tract were established at postmortem examination. There were no morphological changes in tissues of lungs, heart, liver and nephros. Eating of prolonged anthelmintic with feed during 30 days did not render toxicological influence on rats. There were no signs of intoxication, physiological deviations and no animal death. Research results showed that the analyzed prolonged anthelmintic could be referred the substance hazard category 3

Analysis of efficiency of prebiotics and probiotics and different systems of their application for the focused formation of bacteriocenosis of gastro-intestinal tract of calves was realized in the conditions of the Republic of Belarus. There were used 7 groups of calves: 1 – clinically healthy; 2 – calves with diarrheal syndrome which did not get preparations; 3 – calves were administrated daily with liquid probiotic Bifidoflorin Zhidkij in dose 20 ml in course of 20 days; 4 – calves were administrated daily with prebiotic Biofon with colostral milk or milk in dose 20ml in course of 20 days; 5 – calves were administrated daily with prebiotic Biofon-AIL in dose 20 ml in course 20 days; 6 – calves were administrated with probiotic Bifidoflorin Zhidkij simultaneously with prebiotic Biofon; 7 – calves were administrated with probiotic Bifidoflorin Zhidkij in combination with prebiotic Biofon-AIL. In course of studies there was analyzed the age-specific dynamics of bifidus bacteria, lactic acid bacillus, and Escherichia quantities in calves excrements. Research results showed that application of the proposed prebiotics and probiotics promoted the earlier and active contamination of intestines with obligate anaerobe gram-positive flora with its dominative position in relation to gram-negative nonsporeforming facultative anaerobic flora. At the stage of the second aging immunodeficiency the analyzed preparations rendered the resolving effect. The highest positive indexes were obtained after combined application of probiotic Bifidoflorin Zhidkij with prebiotic Biofon-AIL

Research of immune activity of an experimental series of inactivated vaccine against swine pasteurellosis (Pasteurella) with application of adjuvants of Sepptic Montanida ISA 70 and Montanida ISA 206 was realized in the conditions of the Republic of Belarus. In the capacity of water phase there were used serovars A, B, and D Pasteurella multocida. Research results proved the possibility of application of adjuvants of Sepptic company for the development of vaccine against pasteurellosis. The vaccine with the presented adjuvants after its application did not cause after-trouble effects. In course of immunization there was noted the formation long-lived continuous immunity that was proved by high titres of specific anti-bodies in blood serum in all experimental groups of piglets. Single application of the proposed vaccine in doses 2 or 3 ml per animal proved to be effective. Also it was efficient to apply it twice in doses 1 and 2 ml per animal. For the vaccine production it was proposed to use adjuvant Montanida ISA 206 which did not have high reactogenicity

Production examination of efficiency of concentrated vaccine against swine erysipelas from matrix Konev of trial series, control of its immunizing power and production experiments of biological preparation were realized in the conditions of the Republic of Belarus. Research results showed that the presented vaccine had more expressed immunogenic properties in comparison with its production analogue and provided piglets protection against infection. There was analyzed the immunological efficiency of the vaccine: determination of quantity of erythrocytes; leukocytes; content of hemoglobin, level of lysozyme activity, bacterial growth-inhibitory activity, blood serum, phagocytic activity, neutrophilic leukocytes, content of total protein and protein fractions, titre of erysipelas antibodies, and preventive properties of blood serum. Efficiency of biological preparation was determined on the basis of analysis of disease incidence rate, loss of immunized swine caused by swine erysipelas

Evaluation of antagonistic activity of strains of lactic and bifid bacteria, metabolite producers, as well as the treatment and prophylactic efficacy of Laktimet and Batsinil probiotics was realized in the conditions of the Republic of Belarus. In course of study there were estimated 29 strains of lactic acid bacillus of different kinds, 13 strains of bifid bacteria and 35 strains of bacillus (Bac. subtilis and Bac. pumilis). There were studied 3 strains of bifid bacteria: Bifidobacterium adolescentis MS-42; Bifidobacterium adolescentis BIM V-01; Bifidobacterium adolescentis BIM V-87; 2 strains of lactic acid bacillus: Lactococcus lactis subsp. Lactis BIM V-132, Lactococcus casei subsp. rhamnosus BIM V-189; and 6 strains of Bacillus subtilis. Research results showed that the main part of the studied strains had steady antagonistic activity to test-cultures of opportunistic pathogenic bacteria. Zone of growth delay of test-cultures of the main quantity of strains (from 54 up to 92%) was from 11 up to 30. Research results showed that application of cell-free probiotics Laktimet and Batsinil promoted the increasing of resistance by means of increasing or lowering of total protein, insignificant increasing of blood serum bactericidal activity and increasing of phagocytic activity of leukocytes on 7,3%

Creation of effective and easy-to-replicate in laboratory conditions culture medium for isolation of salmonella out of water, which could make it possible to increase the safety, authenticity and efficiency of bacteriological study of aquatic medium objects, was realized in the conditions of the Republic of Belarus. In course of the numorous preliminary experiments there was developed an enrichment medium containing the following: nutrient yeast (5 g/l); potassium phosphate monosubstituted (8,6 g/l); sodium hydrate (1,4 g/l); 0,1% brilliant green solution (5 cm3/l); 0,01% solution of crystal violet (10 cm3/l); distilled water (1 l). The analyzed medium was estimated in accordance with the following propertiesd: medium sensitivity; efficiency; inhibition of bacteria growing. Research result showed that the experimental medium was more sensitive in comparison with the selenite one. Studying of the efficiency of the compared media showed that accumulation of test strains of S. enteritidis on the experimental medium was in 10E4 times higher, S. typhi – in 10E3; S. typhiniurium, S. anatum was in 10 times higher than at selenite media. The experimental studies proved that the experimental culture medium for salmonella isolation out of city water and surface water was more sensitive and easier to use than the selenite medium

Studying of efficiency of application of modified scheme of hyperimmunization of bull-producers with a designer salmonella polyantigenin in comparative analysis with the production scheme of hyperimmunization was realized in the conditions of the Republic of Belarus. Research results showed that engineered polyvalent antigen for hyperimmunization of producer bulls contained in their formula the following serovariants of salmonella in 1:1 ratio: S. typhimurium 371, S. dublin 373, S. choleraesuis 370, and S. enteritidis CB. The obtained antigen was sterile, harmless and had pH 7,3. Hyperimmunization of producer bulls in the experimental scheme revealed in increasing of leukocytes quantity on 57,3 %, banded neutrophils on 86,7 %, B-lymphocytes - on 42,2 %, content of total protein - on 25,6 %, content of Ig G - on 77,8 %, lg M - on 51,6%. After the fifth administration of antigen there was stated the lowering of agglutinating activity of blood serum on 25%, content of Ig G - on 54,4%, Ig M - on 14,6 %, that indicated to the inefficiency of antigen administration and became the basis for termination of hyperimmunization cycle. Immunobiological reaction of bull-producers which were hyperimmunized by the suggested scheme differed from reaction of animals which were hyperimmunized by the production scheme: content of total protein increased on 10,1%, protein content - on 27,3 %, content of lg G - on 24,8 %, lg M - in 23,7%

Investigation of vitamin supply of cattle affected by fascioles in association with strongylatosis of gastro-intestinal tract was realized in the conditions of the Republic of Belarus by an example of white-and-black 3-8 year old cows. Analysis of blood and blood serum of experimental animals showed that content of B1, B2, and E vitamins was lower in group of animals sick with fascioliasis and strongylatosis of gastro-intestinal tract. There were stated no changes in A and C vitamins content. Content of pyroracemic acid exceeded control indexes and was 22,0 +/- 0,173 mg/l. Content of vitamin A did not change and was 0,011 +/- 0,0012 mg/l for sick animals and 0,013 +/- 0,0043 mg/l at healthy animals. Content of vitamin C was different for sick and healthy animals: 1,685 +/- 0,190 mg/l and 1,93 +/- 0,294 mg/l, respectively. Research results proved that treatment of cattle sick with helminth infestation should be realized in complex. It was proposed to apply premixes and vitamin preparations containing B1, B2, as well as A, E, and C vitamins for the increasing of natural protection and for the creation of unfavourable conditions for disease agents

Searching for new, effective and safe methods of treatment of hookworm disease (ancylostomatidosis) of dogs caused by Ancylostoma caninum and Uncinaria stenocephala was realized in the conditions of the Republic of Belarus on the basis of studying of spices composition and dissemination of Ankilostomatidae, age-specific and seasonal disease dynamics, testing of medical and preventive preparations Univerm and Fenbendazol. In the Republic of Belarus Ancylostoma caninum was stated 4,2% of studied cases and Uncinaria stenocephala – in 14,3%. The maximum intensity of the invasion was stated in summer and autumn periods: in cases of individual keeping – 19,4%; in cases of group keeping the disease transformed into enzootic form with 66,7% affect. In winter period there was the lowest invasion – up to 1,4%. The preparation Univerm administrated twofold with feed in dose of 0,1 mg/kg with 24 hours interval proved to be highly effective therapeutic agent with extensefficiency100%. Anthelmintic Fenbendazol administrated once with feed in dose of 0,005 g/kg proved to be highly effective with extensefficiency 80% and intensefficiency 98,84%. Hematological indexes of sick dogs showed the evidence of erythropenia (4,30-4,80 x 10E12/l), hemoglobinemia (113-119 g/l) and eosinophilia (up to 11%), as well as the increased quantity of leukocytes on 5,46% - 23,61 %, and the lowered quantity of lymphocytes on 15,84 % in comparison with healthy animals indexes