The development and availability of follicles is an indicator to predict which of the follicle sizes are suitable to recover the oocytes assisted by means of ultrasonography of transvaginal oocyte retrieval (OPU). Thus, the study was done in order to characterize the follicular recruitment and distribution in response to the repeated removal of follicles, and thus to determine the availability of follicles and immature oocytes harvested repeatedlyfor two consecutive days of OPU in beef cows. Results indicated that 24-h OPU showed significantly greater numbers of medium and large follicles than small categories (P<0.05). However the 48-hr of OPU does not showed any differences of follicles categories (P>0.05). The mean total number of follicles and immature oocytes recovered were higher (P<0.05) in 24-hr OPU (13.76±1.2 and 7.38 ± 1.7) compared to 48-hr OPU (9.08 ± 1.5 and 3.54 ± 1.00) with the oocyte retrieval rate of 51.22% and 38.17%, respectively. The morphological classification indicated the 24-hr oocyte retrieval produced 62% of suitable immature oocytes that can be used for in vitro embryo production. In conclusion, the repeated removal of two consecutive days of OPU has averted the development of dominant follicle, and thus, gave an atmosphere to the subordinate follicles to continue growth relatively to an equal proportion of small, medium and large categories of follicles. Due to the reduction of follicle and recovery rate at 48-hr it is suggested that OPU be carried out later than 48 hour so that the follicle has more time to increase the diameter size.

Seminomas are one of the common testicular tumours that occur mostly in dogs above five years of age. A case of nine-year-old male German Shepherd dog, presented to University Veterinary Hospital, Kokkalai with a history of swelling on the scrotum for the past two months and its successful surgical management is discussed. The right testicle was found to be highly enlarged and hard while the other atrophied. Histopathological examination following its surgical removal, confirmed it as seminoma.

Traceability system for livestock is an important infrastructurenecessary to monitor animal health, animal disease emergencies and quality assurance. The aim of this study was to evaluate farmer and trader willingness to use and pay for an animal identification and traceability system in Peninsular Malaysia. Data was obtained from aquestionnaire that was completed by 543 farmers and traders from the 10 states in the peninsula. Our results show that up to 54% respondents were willing to use and pay for an animal identification and traceability system. Gender and the length of experience of farmers and traders were factors significantly associated with theoutcome of this study. Female farmers and traders were less willing to use and pay for an animal identification and traceability system while farmers and traders with more experience in the cattle industrywere significantly more likely to provide a favourable response.

The Kedah-Kelantan cattle (KK) is an indigenous cattle breed and is mainly kept for meat production in Malaysia. Due to lack of information about polymorphism of growth traits in these cattle, Insulin-like Growth Factor 1 (IGF-1) was chosen to be the candidate gene in this preliminary study. The aim of this study is to investigate the polymorphism of IGF-1 gene in KK and to show that the PCR-RFLP technique can be used as a basis for use as molecular markers in cattle. A total of 46 KK blood samples were collected for DNA extraction performed using a commercial kit. The exon 1 of IGF-1 gene was amplified to produce a 249 bp fragment. The amplified fragments were digested with Eco105I restriction endonuclease and then subjected to electrophoretic separation in Fluorosafe stained 2.5 % agarose gel. The result revealed two alleles, A and B. Threegenotypes were observed: AA, AB and BB. Frequencies were 0.07, 0.13 and 0.80 for AA, AB and BB, respectively. This gives frequencies of 0.13 and 0.87 for A and B alleles. It is concluded that the population is in Hardy-Weinberg disequilibrium (p<0.05). It is possible that this gene has been exposed to selection.

Natural herbal remedies have been long used to control common parasitic infections in livestock. The effectiveness of two plant products was tested on goats with helminthiasis. A study was carried out in a commercial goat farm near Sg. Siput, whereby Azadirachta indica (neem) decoction and leaf extract as well as Jacaranda filicifolia (Jacaranda) leaf extract were fed orally to 3 groups of goats that previously with strongyle egg counts range from 587 to 1650 egg per gram (EPG) for a period of 5 weeks. In this study, theJacaranda Leaves Water Extract (JLWE) showed the highest number of fecal egg count with 2585 EPG at the end of study. All treatments showed reduced percentage of packed cell volume from 23% to 21%,which was not significant. However, there was no difference in the composition of strongyle larvae in the goats. The results show that neem decoction was effective in reducing faecal egg count but the Jacaranda leaf extract and neem leaf extract were not effective in reducing faecal egg counts in goats. Further work is required to assess the efficacy of these herbal products for worm control in goats.

A study was conducted on 16 commercial free range chickens (8 malesand 8 females) sourced from Alor Setar, Kedah in order to determine the prevalence of ecto and endoparasites. Results showed that there were 12 different species of ectoand endoparasites from these chickens. Four (4) species of ectoparasites which consist of three lice and a tick have been discovered. The highest prevalence of ectoparasite was Menopon gallinae (93.8%). The other ectoparasites were Menacanthus pallidulus (81.3%), Haemaphysalis sp. (37.5%) and Lipeurus caponis (18.8%). On the other hand, eight species of endoparasites which consist of four nematodes and four cestodes were discovered. Rallietina echinobothrida showed the highest prevalence of endoparasite (100%) followed by Heterakis gallinarum (93.8%), Acuaria spiralis (87.5%), Ascaria galli (81.3%), Rallietina tetragona(43.8%), Gongylonema ingluvicola (37.5%), Hymenolepis carioca (12.5%)and Hymenolepis cantiana (12.5%). Endoparasites infestation was recorded highest on male chicken (52.6%) compared to female (47.4%). However, there was no statistically significant difference betweenthe number of endoparasites and both sexes; t (14) = 0.817, p>0.05.

The first pilot study of Nonsurgical castration of male long tailedmacaque was carried out in the middle of 2010 in Taman Tasik Perdana (National Lake Garden) in Federal Territory Kuala Lumpur. Intraepididymal injections method such as chemical castration wasused as one of the fertility control in adult male long tailed macaques. This method does not require removal of the testis, easy to do and inexpensive and mixture of Ethanol-Formalin was used inthis technique. Outcome from the male macaque castration programme shows this sterilization technique using a mixture of ethanol and formalin to be very effective in controlling new birth. Castrated male macaque doesn’t have any changes in behaviour or physical attributes. These results prove that the breeding of macaques can be reduced.

A primary fibroblast cells from embryos of brown quail Coturnixypsilophora has been established and partially characterized. The cells were maintained in Modified Eagle’s medium (MEM) supplemented with 10% fetal bovine serum. The cells were able to grow at temperatures between 35°C and 38°C with optimum temperature of 37°C. The growth rate of primary quail fibroblast cells increased as the FBS proportion increased from 5% to 20% at 37°C with optimum growth at the concentrations of 10% or 15% FBS. The cells showed no microbial contamination throughout the period of experiment and the total chromosome number of a diploid cell was 78, according to karyotyping and chromosome analysis. The susceptibility of quail primary cells for avian viruses was investigated in this study after inoculation with ND and IB viruses. Both viruses showed a satisfactory CPE development and the infectivity were assayed by virus titration (TCID50). This suggests that the quail primary cells can be used for isolation of various avian viruses with further steps of infectivity confirmation in the future.

A mixed viral infection of Newcastle Disease (ND), Marek’s Disease (MD) and Avian Leukosis (AL) was reported in village chickens. Analysis of the deduced amino acid sequences of the F protein cleavage site of ND virus showed that the isolate was virulent with sequence KRRKR for the C-terminus of the F2 protein and phenylanine (F) at residue 117, the N-terminus of the F1 protein. Basic Local Alignment Search Tool (BLAST) analysis showed the isolate was ND which was 96% similar identity with Indonesia Sukorejo genotype VII, MD 99% similar identity with China very virulent strain and ALV 100% similar identity with Taiwan ALV strain. Due to the free ranging type of management, causes of the diseases in the poultry were uncertain as many factors can contribute to the disease occurrence. However, good hygiene practices can help to improve the farm’s sanitary. Basic biosecurity system can be applied although the success may be limited. Vaccination is another alternative that can be considered to prevent the diseases with the help from certain parties.

This report describes the parasitological and pathological information retrieved from the samples submitted to Regional Veterinary Laboratory, Bukit Tengah, Penang (MVKBT) followingan episode of histomoniasis in a small scale poultry farm. Liver and ceca from two dead turkey birds, water (n=5) and freshwater snails (Pomacea sp, n=7) were stored between 4°C to 10°C until analyzed. Soil samples (n=7) were preserved in 5% formalin and kept at room temperature prior to further testing. The soil samples were filtered through laboratory test sieves before the filtrates at each level were examined using direct microscopy. Portions of the liver were processed using routine paraffin technique for histopathological examination and found positive for Histomoniasis. Results from direct microscopy of the liver samples revealed Schistosoma mansoni ova, Sarcocystis sporocysts and Fasciola sp. Heterakis sp ova were detected from cecal content. Four out of seven soil samples were positive for Sarcocystis sporocysts, Coccidia oocyst, Strongyles ova and nematod larva. Water and freshwater snail samples were negative for cercaria of Schistosoma mansoni. This case was concluded as Histomoniasis, Sarcocystosis and helminthiasis with incidental finding of Schistosoma mansoni.