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A study of the bone marrow derived mesenchymal stromal cells in rats – proliferation and immunophynotypic markers Texte intégral
2018
Mauida, F. Hasoon, | Buktiar, Nader | Majed, H. Mohammed
Mesenchymal stem cells derived from bone marrow (BM-MSCs) havethe ability to differentiate into multiple cell lineages. Although the cultivation of these cells has led to a number of characterisation studies, some significant morphological and immunohistochemical properties are still lacking. In this study, isolation of BM-MSCs, morphological features, cell viability, immunophenotypic properties and cryopreservation of BM-MSCs wereexamined in detail. The results demonstrate that the cells isolated from BM-MSCs were plastic adherent and had fibroblastic spindle shape after three passages and get confluent monolayer cells 70-80% after 4-7 days post-subculture. Based on the cell viability analysis, the BM-MSCs showed an increase in cell viability starting from passage 1 until passage 10. Immunophenotypic analysis demonstrated that BM-MSCs were positivefor CD44 and CD105 and negative for CD34. Functional analysis of cryopreservation of BM-MSCs from P6 after 6 months expressed good proliferation rate and cell viability.
Afficher plus [+] Moins [-]Incidence of Peste des Petits Ruminants in Rangpur sadar of Bangladesh Texte intégral
2018
Rahman, Md Mamunur | Hassan, Md Zakir | Sultana, Salma | Uddin, Md Karim | Hossain, SM Sarwar
The study was conducted to determine the prevalence of Peste des Petits Ruminants in different breed of goats based on age, sex, seasonal variation and vaccination status at Upazilla Veterinary Hospital, Pirojpur Sadar, Bangladesh during the period from January 2015 to December 2015. A total of 319 cases of different breed of goats were recorded in which 43 (13.48%) were infected by Peste des Petits Ruminants. The diagnosis of Peste des Petits Ruminants was performed based on clinical history, clinical signs and some laboratory techniques. The major clinical signs were high fever, nasal discharge, rapid breathing, mouth lesions and bloody diarrhea.The prevalence was highest in Black Bengal goat (6.90%) compare to Jamunapari (3.76%) and crossbreds (2.82%). Female goats were more susceptible (60.47%) than the male goats (39.53%). 5 months to 11 months aged goats showed highest prevalence (48.84%) followed by 1 to 3 years aged goats (27.91%) and 0 to 4 months aged goats (23.26%). In winter season, the prevalence was highest (55.81%) compare to summer (25.58%) and rainy season (18.60%). The non-vaccinated goats were more susceptible (90.91%) than the vaccinated goats (9.09%).Those prevalence of Peste des Petits Ruminants in different breed of goats based on age, sex, seasons and vaccination will help clinician to know the occurrence of Peste des Petits Ruminants in this area and will help them to take proper preventive measures.
Afficher plus [+] Moins [-]A case of Neospora caninum infection in a dog Texte intégral
2018
Kahraman, Duygu | Ceylan, Ebubekir
Infection with Neospora caninum in a dog is described. The predominant clinical findings were proprioception deficit, involuntary and continuous contracture of the pelvic extremity muscles. N. caninum is structurally similar to Toxoplasma gondii, but it is immunologically distinct. Therefore, infections had been misdiagnosed as toxoplasmosis. The diagnosis was confirmed by an immunofluorescence antibody test. The parasite is sensitive to clindamycin and trimethoprim/sulphonamide, however the prognosis is poor especially if the muscle contractures have occurred.
Afficher plus [+] Moins [-]The Diagnostic Significance of Serum Troponin Levels Lambs with White Muscle Disease Texte intégral
2018
Basbugan, Yildiray | Yuksek, Nazmi | Altug, Nuri | Ceylan, Ebubekir | Yoruk, Ibrahim Hakki | Agaoglu, Zahit Tevfik
This study was carried out to determine the prognostic importance of troponin enzyme levels in lambs with White Muscle Disease (WMD). Materials and Methods: This study consisted of 50 male and female lambs aged 0-3 months old, 30 of which had clinical white muscle disease (Group I) and 20 of which were healthy (Group II – Control Group). Group I was composed of lambs that showed clinical symptoms of the disease. The disease was also identified by laboratory analysis. The lambs in this group were treated and the course of the disease was followed for 15 days.Compared with the control group, the Group I before treatment results were as follows: AST, LDH, CK, CK-MB, Troponin I and T (P<0.001) were high, while GSH-Px (P<0.001), SOD (P<0.01), Se (P<0.01), Retinol and Tocopherol were low. Compared with the control group, the Group I After Treatment results were as follows: AST, CK, Troponin T (P<0.001) were high, LDH (P<0.01) was high and GSH-Px (P<0.01) was low. Comparing Group I Before Treatment and After Treatment results, AST, CK, CK-MB and Troponin I (P<0.001), as well as GSH-Px, SOD and Se (P<0.01) were high. It was determined that LDH decreased and Tocopherol increased. Whereas Vitamin D3 was determined to be insignificant in all three groups, the levels of CK-MB, Troponin I, Retinol, SOD and Se were insignificant in the After Treatment and control groups, and the Troponin T and retinol values were measured to be insignificant in the Before Treatment and After Treatment groups.To conclude, measuring the AST, CK, CK-MB, LDH values as well as Troponin can be an important parameter for the identification and prognosis of WMD.
Afficher plus [+] Moins [-]Ectoparasites of Domestic Pigeons (Columba livia domestica) in Antakya Region Texte intégral
2018
Elmacioglu, Sibel | Yaman, Mehmet | Zerek, Aykut | Akkucuk, Serife | Karagoz, Mustafa | Erdem, Ipek
Besides the yield loss of the pigeons those are infested with ectoparasites they are also susceptible against the other infections because of weakened immunity. Thus it has been evaluated the situation of ectoparasites of domestic pigeons and determined the prevalance in Antakya region. Materials and Totally 100 domestic pigeons (Columba livia domestica) which are fed for hobby in Antakya region were examined with respect of ectoparasites. The prevalance of ectoparasites of domestic pigeons has been determined and identified according to the literature.The prevalance of ectoparasites of domestic pigeons was determined as 52% and three different ectoparasites were detected. Columbicola columbae was the dominant species with a percentage of 45%, Argas reflexus and Pseudolynchia canariensis were also dedected with a percentage of 5% and 2%, respectively. Despite the higher percentage of infestation in female pigeons (54,5%) when compared with males (48,9%) and adults (56,9%) when compared with juveniles (42,9%) no significant difference was found statistically (p>0.05). But louse infestation of adult pigeons (58,3%) was significantly higher than the juvenile pigeons (28,5%) statistically (p<0.05).The yield and perfomance loss in domestic pigeons should not be ruled out. Therefore hygien of shelters and ventilation is crucially important. Ectoparasiter situation should be monitorized and gotten under controlled.
Afficher plus [+] Moins [-]Prevalence of methicillin-resistant Staphylococcus aureus among large commercial pig herds in South Africa Texte intégral
2018
van Lochem, Shani(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies) | Thompson, Peter N.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies) | Annandale, Cornelius H.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies)
Prevalence of methicillin-resistant Staphylococcus aureus among large commercial pig herds in South Africa Texte intégral
2018
van Lochem, Shani(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies) | Thompson, Peter N.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies) | Annandale, Cornelius H.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies)
The prevalence of nasal carrier status of methicillin-resistant Staphylococcus aureus (MRSA) in pigs has been described elsewhere, but is unknown in South Africa. To address concerns that exist regarding the zoonotic risk that carriers pose to workers, the herd-level prevalence of MRSA was determined among 25 large (> 500 sows) commercial pig herds in South Africa, representing 45% of the large commercial herds in the country. From each herd, the nasal contents of 18 finisher pigs were sampled at the abattoir, pooled into three and selectively cultured to determine the presence of MRSA. A herd was classified as MRSA-positive if one or more of the three pooled samples cultured positive. Three of the 25 herds tested positive for MRSA, equating to a 12% herd prevalence (95% CI: 7% - 23%) among South African commercial piggeries. The prevalence of nasal MRSA carriers among large commercial pig herds in South Africa was low compared to what has been reported elsewhere and suggests a relatively low zoonotic MRSA risk to workers in South African commercial piggeries and abattoirs.
Afficher plus [+] Moins [-]Prevalence of methicillin-resistant Staphylococcus aureus among large commercial pig herds in South Africa Texte intégral
2018
Shani van Lochem | Peter N. Thompson | Cornelius H. Annandale
The prevalence of nasal carrier status of methicillin-resistant Staphylococcus aureus (MRSA) in pigs has been described elsewhere, but is unknown in South Africa. To address concerns that exist regarding the zoonotic risk that carriers pose to workers, the herd-level prevalence of MRSA was determined among 25 large (> 500 sows) commercial pig herds in South Africa, representing 45% of the large commercial herds in the country. From each herd, the nasal contents of 18 finisher pigs were sampled at the abattoir, pooled into three and selectively cultured to determine the presence of MRSA. A herd was classified as MRSA-positive if one or more of the three pooled samples cultured positive. Three of the 25 herds tested positive for MRSA, equating to a 12% herd prevalence (95% CI: 7% – 23%) among South African commercial piggeries. The prevalence of nasal MRSA carriers among large commercial pig herds in South Africa was low compared to what has been reported elsewhere and suggests a relatively low zoonotic MRSA risk to workers in South African commercial piggeries and abattoirs.
Afficher plus [+] Moins [-]Immunogenicity of a plasmid DNA vaccine encoding G1 epitope of bovine ephemeral fever virus G glycoprotein in mice Texte intégral
2018
Pasandideh, Reza(Natural Resources University Department of Animal Science, Khuzestan Agricultural Sciences) | Shapouri, Masoud Reza Seyfi Abad(Shahid Chamran University of Ahvaz Department of Pathobiology) | Nassiri, Mohammad Taghi Beigi(Natural Resources University Department of Animal Science, Khuzestan Agricultural Sciences)
Immunogenicity of a plasmid DNA vaccine encoding G1 epitope of bovine ephemeral fever virus G glycoprotein in mice Texte intégral
2018
Pasandideh, Reza(Natural Resources University Department of Animal Science, Khuzestan Agricultural Sciences) | Shapouri, Masoud Reza Seyfi Abad(Shahid Chamran University of Ahvaz Department of Pathobiology) | Nassiri, Mohammad Taghi Beigi(Natural Resources University Department of Animal Science, Khuzestan Agricultural Sciences)
The aim of this study was to investigate the immunogenicity of a plasmid deoxyribonucleic acid (DNA) vaccine encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein in mice. A plasmid DNA carrying the G1 gene was constructed and designated as pcDNA3.1-G1. The expression of the target gene was confirmed in human embryonic kidney 293 (HEK 293) cells transfected with pcDNA3.1-G1 by indirect immunofluorescent staining. Immunisation experiments were intramuscularly carried out by vaccinating 6-week-old female mice in four groups, including the pcDNA3.1-G1 construct, pcDNA3.1 (+) plasmid alone, BEF-inactivated vaccine and phosphate-buffered saline (PBS) (1X) three times with 2-week intervals. Fourteen days after the last immunisation, the animals were bled and the resulting sera were tested for anti-G1-specific antibodies by immunoblotting analysis, indirect enzyme-linked immunosorbent assay (ELISA) and virus neutralisation (VN) test. Serological assays showed that the pcDNA3.1-G1 construct expressing G1 protein was able to elicit specific antibodies against this antigen. Virus neutralisation test showed that pcDNA3.1-G1 could induce anti-BEFV-neutralising antibodies in mice. Our findings indicated that a new dimension can be added to vaccine studies for bovine ephemeral fever (BEF) using eukaryotic expression plasmids encoding the G1 antigen in the future.
Afficher plus [+] Moins [-]Immunogenicity of a plasmid DNA vaccine encoding G1 epitope of bovine ephemeral fever virus G glycoprotein in mice Texte intégral
2018
Reza Pasandideh | Masoud Reza Seyfi Abad Shapouri | Mohammad Taghi Beigi Nassiri
The aim of this study was to investigate the immunogenicity of a plasmid deoxyribonucleic acid (DNA) vaccine encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein in mice. A plasmid DNA carrying the G1 gene was constructed and designated as pcDNA3.1-G1. The expression of the target gene was confirmed in human embryonic kidney 293 (HEK 293) cells transfected with pcDNA3.1-G1 by indirect immunofluorescent staining. Immunisation experiments were intramuscularly carried out by vaccinating 6-week-old female mice in four groups, including the pcDNA3.1-G1 construct, pcDNA3.1 (+) plasmid alone, BEF-inactivated vaccine and phosphate-buffered saline (PBS) (1X) three times with 2-week intervals. Fourteen days after the last immunisation, the animals were bled and the resulting sera were tested for anti-G1-specific antibodies by immunoblotting analysis, indirect enzyme-linked immunosorbent assay (ELISA) and virus neutralisation (VN) test. Serological assays showed that the pcDNA3.1-G1 construct expressing G1 protein was able to elicit specific antibodies against this antigen. Virus neutralisation test showed that pcDNA3.1-G1 could induce anti-BEFV-neutralising antibodies in mice. Our findings indicated that a new dimension can be added to vaccine studies for bovine ephemeral fever (BEF) using eukaryotic expression plasmids encoding the G1 antigen in the future.
Afficher plus [+] Moins [-]Effects of MK-467 hydrochloride and hyoscine butylbromide on cardiorespiratory and gastrointestinal changes induced by detomidine hydrochloride in horses Texte intégral
2018
Tapio, Heidi A. | Raekallio, Marja R. | Mykkanen, Anna | Mama, Khursheed | Mendez-Angulo, Jose L. | Hautajavi, Heidi | Vainio, Outi M.
OBJECTIVE To compare the effects of MK-467 and hyoscine butylbromide on detomidine hydrochloride–induced cardiorespiratory and gastrointestinal changes in horses. ANIMALS 6 healthy adult horses. PROCEDURES Horses received detomidine hydrochloride (20 μg/kg, IV), followed 10 minutes later by MK-467 hydrochloride (150 μg/kg; DET-MK), hyoscine butylbromide (0.2 mg/kg; DET-HYO), or saline (0.9% NaCl) solution (DET-S), IV, in a Latin square design. Heart rate, respiratory rate, rectal temperature, arterial and venous blood pressures, and cardiac output were measured; blood gases and arterial plasma drug concentrations were analyzed; selected cardiopulmonary variables were calculated; and sedation and gastrointestinal borborygmi were scored at predetermined time points. Differences among treatments or within treatments over time were analyzed statistically. RESULTS With DET-MK, detomidine-induced hypertension and bradycardia were reversed shortly after MK-467 injection. Marked tachycardia and hypertension were observed with DET-HYO. Mean heart rate and mean arterial blood pressure differed significantly among all treatments from 15 to 35 and 15 to 40 minutes after detomidine injection, respectively. Cardiac output was greater with DET-MK and DET-HYO than with DET-S 15 minutes after detomidine injection, but left ventricular workload was significantly higher with DET-HYO. Borborygmus score, reduced with all treatments, was most rapidly restored with DET-MK. Sedation scores and pharmacokinetic parameters of detomidine did not differ between DET-S and DET-MK. CONCLUSIONS AND CLINICAL RELEVANCE MK-467 reversed or attenuated cardiovascular and gastrointestinal effects of detomidine without notable adverse effects or alterations in detomidine-induced sedation in horses. Further research is needed to determine whether these advantages are found in clinical patients and to assess whether the drug influences analgesic effects of detomidine.
Afficher plus [+] Moins [-]Effects of the peripherally acting α2-adrenoceptor antagonist MK-467 on cardiopulmonary function in sheep sedated by intramuscular administration of medetomidine and ketamine and reversed by intramuscular administration of atipamezole Texte intégral
2018
Adam, Magdy | Raekallio, Marja R. | Salla, Kati M. | Honkavaara, Juhana M. | Mannikko, Sofia | Scheinin, Mika | Kajula, Marena | Molsa, Sari H. | Vainio, Outi M.
OBJECTIVE To evaluate effects of the peripherally acting α2-adrenoceptor antagonist MK-467 on cardiopulmonary function in sheep sedated with medetomidine and ketamine. ANIMALS 9 healthy adult female sheep. PROCEDURES Each animal received an IM injection of a combination of medetomidine (30 μg/kg) and ketamine (1 mg/kg; Med-Ket) alone and Med-Ket and 3 doses of MK-467 (150, 300, and 600 μg/kg) in a randomized blinded 4-way crossover study. Atipamezole (150 μg/kg, IM) was administered 60 minutes later to reverse sedation. Cardiopulmonary variables and sedation scores were recorded, and drug concentrations in plasma were analyzed. Data were analyzed with a repeated-measures ANCOVA and 1-way ANOVA. Reference limits for the equivalence of sedation scores were set at 0.8 and 1.25. RESULTS Heart rate, cardiac output, and Pao2 decreased and mean arterial blood pressure, central venous pressure, and systemic vascular resistance increased after Med-Ket alone. Administration of MK-467 significantly alleviated these effects, except for the decrease in cardiac output. After sedation was reversed with atipamezole, no significant differences were detected in cardiopulmonary variables among the treatments. Administration of MK-467 did not significantly alter plasma concentrations of medetomidine, ketamine, norketamine, or atipamezole. Sedation as determined on the basis of overall sedation scores was similar among treatments. CONCLUSIONS AND CLINICAL RELEVANCE Concurrent administration of MK-467 alleviated cardiopulmonary effects in sheep sedated with Med-Ket without affecting sedation or reversal with atipamezole.
Afficher plus [+] Moins [-]Associations between dialysate sodium concentration and plasma sodium concentration of dogs receiving intermittent hemodialysis treatments Texte intégral
2018
Foster, Jonathan D. | Drobatz, Kenneth J. | Cowgill, Larry D.
OBJECTIVE To compare dialysate sodium concentration and patient plasma sodium concentration of dogs during intermittent hemodialysis treatments. SAMPLE 211 intermittent hemodialysis treatments performed on 40 client-owned dogs for the management of dialysis-dependent uremia. PROCEDURES Medical records were reviewed to determine the plasma sodium concentration of each dog before and after routine hemodialysis treatments. Associations between detected changes in plasma sodium concentration and dialysate sodium concentration were evaluated by use of Spearman rank correlations and linear regression analysis. RESULTS Significant linear correlations were found between the dialysate sodium concentration and patient sodium concentration. The starting dialysate-to-patient sodium gradient was associated with the strongest correlation to the change in patient sodium concentration at the end of the dialysis session. Modest correlations existed between the dialysate sodium concentration and postdialysis patient sodium concentration as well as between the predialysis dialysate-to-patient sodium gradient and postdialysis dialysate-to-patient sodium gradient. CONCLUSIONS AND CLINICAL RELEVANCE The dialysate sodium concentration was correlated with the patient sodium concentration in dogs, and the dialysate-to-patient sodium gradient could be used to further refine this association to predict the postdialysis patient sodium concentration and potentially manage dysnatremia during hemodialysis. Prospective studies should be performed to determine how these associations can be used to correct aberrations as well as to avoid unwanted alterations in patient sodium concentrations.
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