Metformin is a treatment used widely for non-insulin-dependent diabetes mellitus with few side effects and acts by inhibiting hepatic gluconeogenesis and glucose absorption from the gastrointestinal tract. Lymphoma is one of the most common hematological malignancies in dogs. Chemotherapy is used mainly on lymphoma, but further research on developing anticancer drugs for lymphoma is needed because of its severe side effects. This study examined the anticancer effects of metformin alone and in combination with 2-deoxy-D-glucose (2-DG), a glucose analog, on EL4 cells (mouse T cell lymphoma). Metformin reduced the metabolic activity of EL4 cells and showed an additive effect when combined with 2-DG. In addition, cell death was confirmed using a trypan blue exclusion test, Hochest 33342/propidium iodide (PI) staining, and Annexin V/PI staining. An analysis of the cell cycle and mitochondria membrane potential (MMP) to investigate the mechanism of action showed that metformin stopped the G2/M phase of EL4 cells, and metformin + 2-DG decreased MMP. Metformin exhibited anticancer effects as a G2/M phase arrest mechanism in EL4 cells and showed additive effects when combined with 2-DG via MMP reduction. Unlike cytotoxic chemotherapeutic anticancer drugs, metformin and 2-DG are related to cellular glucose metabolism and have little toxicity. Therefore, metformin and 2-DG can be an alternative to reduce the toxicity caused by chemotherapeutic anticancer drugs. Nevertheless, research is needed to verify the in vivo efficacy of metformin and 2-DG before they can be used in lymphoma treatments.

An adult raccoon dog with extensive, deep, and contaminated wounds on the right hip and multiple fractures was rescued. The open wound was managed daily by debridement and flushing for 3 weeks. Modified active drainage was then performed, and antibiotics administered according to the antibiotic susceptibility test. After 2 weeks, the exudate disappeared and the drain was removed. After monitoring for 1 month, the animal was released in to the wild. This case shows that even if infection remains, rapid wound repair is possible if appropriate antibiotic selection through regular examination and active drainage are combined.

Many viruses can infect different types of birds, with poultry being the most susceptible. These viral diseases have a direct negative impact on the poultry industry, with significant economic losses. This study examined a group of the most important viruses that infect backyard chickens in 2 specific areas of Basrah Governorate, south of Iraq. The study analyzed avian influenza viruses (AIVs), Newcastle disease virus (NDV), and infectious bronchitis virus (IBV). Two hundred and ninety oropharyngeal swabs, 150 from Abu Al-Khasib and 140 from Shatt Al-Arab regions in the Basrah governorate, were obtained from backyard chickens with clear respiratory signs. The samples were subjected to viral RNA extraction, and the viral nucleic acids were detected using a reverse transcriptase polymerase chain reaction technique. The overall rate of viral infections was 74.8%, which varied depending on the type of virus: 15.8%, 31.3%, and 27.5% for AIV, NDV, and IBV, respectively. The NDV and IBV had much higher infection rates than that of AIV. In addition, the prevalence of AIV in the Shatt Al Arab district was significantly higher than in the Abul Khasib district. Moreover, there were no significant differences between the NDV and the IBV distributions in either of the targeted regions in this study.

Non-human primate (NHP) research faces challenges due to zoonosis risk and complex veterinary management yet lacks standardized guidelines for animal care. Therefore, we developed an advanced veterinary management protocol for NHP quarantine, anesthesia, and postoperative care. Three female 4 to 5-year-old cynomolgus monkeys were anesthetized and underwent various tests, including body weight, temperature, blood tests, urinalysis, microbiological monitoring, and physical and dental examinations. Ivermectin and medicated baths were administered to eradicate ectoparasites and endoparasites, and testing was repeated 30 days later. Following quarantine, we performed computed tomography and anesthesia maintenance for mastoidectomy. To relieve pain and maintain body weight, we administered tramadol intramuscularly 4 times/day for 3 days and meloxicam subcutaneously twice daily for 14 days. Feed replacements were provided. During the 33-day quarantine period, physical examinations revealed no abnormalities indicative of infectious diseases, and no specific clinical symptoms were observed. Through a preliminary test of anesthesia time, we selected ketamine 4 mg/kg + medetomidine 50 µg/kg for short experiments such as computed tomography, and ketamine 8 mg/ kg + medetomidine 50 µg/kg for intubation. Ten days after mastoidectomy, NHPs consumed 100 kcal/kg and recovered their body weight. This study offers advanced veterinary management guideline for NHP research. Such protocols can lead to more standardized and ethical practices in NHP research, thereby enhancing the quality of studies on NHPs and the translation of findings to human health and disease.

From 2008 to 2017, blood samples from 280 and fecal samples from 149 stray cats in Gwangju, South Korea, were examined for feline immunodeficiency virus (FIV), feline leukemia virus (FeLV), Dirofilaria immitis, and Giardia infections using commercial diagnostic tests. Overall, the combined prevalence of FeLV, FIV, D. immitis, and Giardia was 8.6%, 1.4%, 0.4%, and 2.0%, respectively. FeLV exhibited the highest prevalence rate among the 4 pathogens surveyed, both in the 2008–2009 (9.6%) and 2015–2017 (6.3%) surveys. The results of the feline Giardia study represent the first prevalence report of Giardia infection among stray cats in Korea.

Granular cell tumor was described in the testis of two rabbits. Testis from each rabbit was surgically removed and submitted for histopathological diagnosis. Both testes were about 2.0 cm in diameter, firm, and tan. Microscopically, testicular mass consisted of compact sheets of round to polygonal and occasional spindle-shaped cells. The neoplastic cells contain a large amount of eosinophilic granular material in the cytoplasm. The cytoplasmic eosinophilic granules were positive for periodic acid Schiff stain. Immunohistochemically, the neoplastic cells were immunoreactive to Melan-A and vimentin. Based on these results, the testicular mass was diagnosed as a granular cell tumor.

Repeat breeding due to sub clinical endometritis (SCE) is often diagnosed by cytological examination and the effect of endocervical inflammation (ECI) on increased hazards ofpregnancy is inadequately investigated. In this study, endocervical cytology was compared with the golden standard test `endometrial cytology' to detect SCE as a cause of repeat breeding in cows. Influx of Neutrophils and endocervical inflammation will reflect the status of endometrium and may indirectly reflects the ongoing sub clinical form of endometrial inflammation. Hence, this study was aimed at fixing threshold for PMN in endocervical cytology and comparing the same with golden standard technique. Repeat breeding cows associated with endocervical inflammation with >8% of neutrophils during standing estrus in endocervical cytology will have increased hazards of pregnancy. Our study suggested that a moderate concordance/association between ECI and endometrial inflammation (EDI) in repeat breeding cows, these findings were based on the endocervical and endometrial cytology. Based on the endocervical cytology examination 85% of repeat breeding cow posses EDI (SCE) with a diagnostic sensitivity of 86% and showing moderate clinical acceptability for application of endocervical cytology as diagnostic aid to detect SCE in repeat breeding cows.

The present study was undertaken to analyze the factors determining the participation of farmers in contract Japanese quail farming. The primary data for the study was collected through a pre-tested interview schedule. The determinant factors for farmers participation in contract Japanese quail farming were assessed by the logit model and the results of the analysis revealed that the farm size and profitability were found to be the positive determinant factors and investment was found to be a negative determinant factor for the participation of farmers in contract Japanese quail farming.

Composite cattle feed samples and raw materials like maize, wheat, sorghum, broken rice, pearl millet, groundnut cake, cotton seed cake, mustard cake, soyabean meal, rapeseed meal, sunflower meal, guar meal, safflower meal, deoiled rice bran and wheat bran were collected from various parts of India for a period 5 years and evaluated for aflatoxin B1 level by screening through ELISA and confirmation through HPLC. Among the 482 composite cattle feed samples, only 68.5per cent were having aflatoxin Bilevel below the permissible level (below 20 ppb). Higher incidence of aflatoxin B1 was recorded in west zone than east, north and south zones during monsoon period. However, it was observed that incidence of aflatoxin B1 at concentration beyond 100ppb was relatively higher in eastern zone both during monsoon and non-monsoon period than the west, north and south zones. Among the energy source, maize had the highest (7.8 per cent, 15 out of 193 samples tested) prevalence of alarming levels of aflatoxin (>100ppb) and 41.5 per cent (80 samples) in the range of 21 to 100 ppb of aflatoxin B1. Only 50.8 per cent (98 samples) were within permissible level (<20 ppb). It was observed that the presence of aflatoxin Biwas more in maize during monsoon in south zone. Only 47.2 per cent of groundnut cake (118 samples) were within permissible level of <20 ppb of aflatoxin B1. In west zone, highest sample profile (15.18per cent) and zone-wise distribution of 45.45per cent was recorded in the samples of groundnut cake having aflatoxin B1 at alarmingly high level of more than 100 ppb. Overall data reveals that monsoon favours development of aflatoxin B1 more during monsoon.

A study was conducted to optimize the level of finger millet (Eleusine coracana) powder as a thickening agent in the chicken soup prepared with no added oil. Whole finger millet (FM) was powdered finely and used as thickening agent in chicken soup at 0 (control) 5(FM5), 10(FM10) and 15(FM15) parts levels. The pH significantly decreased with increase in the finger millet level where it was highest in control and lowest in FM15. In the sensory evaluation, appearance, flavour and mouth feel scores significantly decreased with increase in finger millet level. The consistency scores were significantly higher for the treatments with finger millet compared to control. The overall acceptability scores were comparable among the control, FM5 and FM10 treatments and significantly lower for FM15. Hence, from this study, it can be concluded that finger millet might be efficiently utilized as a thickening agent in chicken soup up to 10 parts level, which would fortify the nutritive value.