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The Protective Effect of Alpha-Lipoic Acid on Oxidative Stress and Proinflammatory cytokines in L-Arginine-Induced Acute Pancreatitis Male Rats Texte intégral
2022
Nuha Abeed | Muna AL-Saeed
Acute pancreatitis (AP) is a common serious stirring disease of the pancreas characterized by strict abdominal twinge that lasts for days to weeks. Rats were injected with high dose of the amino acid L-arginine (500 mg/kg B.W.). The study has been done to induce acute pancreatitis (AP) by L-Arginine (Arg) and to investigate the effect of AP on body weight, body weight gain, serum amylase, lipase, oxidative stress and proinflammatory cytokine parameters. In addition, some histopathological changes in pancreas and intestine were examination in male rats. Serum amylase, lipase, IL-6, and TNF-α levels, and MDA levels significantly increased in rats with L-Arginine induced AP. However, the boy weight and body weight gain, serum GPx, SOD and CAT activity significantly reduced. In addition, some histological changes in pancreas and intestine were identified, reversed and improved with ALA treatment.
Afficher plus [+] Moins [-]The Effect of Egg Shape (egg Quality Traits) Parameters on The Characteristics of Hatched Chicks by Local Chicken Texte intégral
2022
Shokhan Ali | Chro Aziz | Jaza Mohammed | Mohammed Mohammed | Questan Ameen | Ahmed Shaker
The current study was done in the hatchery unit/ animal production department/ directorate of agricultural research in Sulaimani province from 28 March – 7 May 2022. Three hundred (300) eggs were used to study the effect of egg shape parameters on the characteristics of hatched chicks. Immediately after collecting the eggs, the eggs were weighted by using electronic balance (0.01 g) sensitive, and the length and Breadth for each egg was measured by using digital caliper vernier with (0.01 mm) sensitive. Our result indicates that the chick color was affected by the egg weight and the egg breadth (P<0.05) but another studied traits was not significantly affected on the characteristics of hatched chicks.
Afficher plus [+] Moins [-]Synthesis, Characterization, Acute Toxicity and Investigation of the new Pyrimidine Derivative as antihyperlipidemic activity on Male Mice. Texte intégral
2022
Mohsen Brage | Adel AL Zobidy | Wasfi Al-Masoudi
A new pyrimidine derivative was prepared by reaction of Pyridimine-4,5-diaminewith 3-Phenyl propanal in good yield. This new Schiff base was characterized by elemental analysis, Infra-red and Proton Nuclear Magnetic Resonance (1H- NMR) spectroscopy. The toxicity of the compound was also assayed via the determination of their LD50 value by using Dixon method. LD50 of the novel compound was 618.75 mg/Kg BW when administered Intraperitonially. In Atorvastatin has been used as a controlled drug for the treatment of HFD-induced hyperlipidemia as improvement drug, both atorvastatin and pyrimidine derivative prevented the increase in serum and hepatic levels of total cholesterol (TC), total glycerides (TGs), low density lipoprotein (LDL-C), and increased serum and hepatic levels of high-density lipoprotein (HDL-C) in HLD-fed rats. The results of the histopathological study of sections of the liver of the study groups confirmed their conformity with the physiological study.
Afficher plus [+] Moins [-]Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq Texte intégral
2022
Ali Radhi | Israa Alsaad
The present study aimed to detection and identified the infection of Mycoplasma haemocanis in dogs through clinical manifestation, blood film, conventional PCR technique and sequences analysis. The total number of dogs examined clinically were one hundred, and 25 clinically healthy control in southern Iraq provinces. Examined dogs showed various clinical signs. Blood samples were taking for hematological and biochemical analysis. 72 (72%) of blood smear were positive for Mycoplasma haemocanis, whereas 33(45.8%) positive based on conventional PCR technique. hematological examination in infected animals showed a significant decrease in mean of TRBC, Hb and PCV and significant increase (p>0.05) in the TLC. biochemical examination indicates a significant increase in AST, ALP and ALT in infected animals. The local Mycoplasma haemocanis were showed genetic variant related to NCBI-BLAST Mycoplasma haemocanis Turkey, Thailand, Brazil, and India isolates. In conclusion Mycoplasma haemocanis have been diagnosed in dogs in Basrah city with various clinical manifestation from in apparent to sever anemia, emphasized the infection using conventional PCR technique, and Phylogenetic analysis confirm the identification of Mycoplasma haemocanis as a new submission of local Iraq, with 99% identical to India, Brazil, Thailand and Turkey DNA sequence.
Afficher plus [+] Moins [-]Histopathological Study of some Abnormalities of Infertility in the Queens Texte intégral
2022
Abeer Yaseen | Dhuha Kareem | Zainab Waheed | Taher fahad
The present study deals with causes of infertility abnormalities in queens. The sample of the present study consists of eight of the female reproductive system of queens. The sample of this study was collected from queens of different places in Basra randomly. Then the female reproductive system of queens was completely removed by the operation of ovario- Hysterectomy in which this operation was done weekly in the laboratory of surgery in Basra Veternerary Medicine,The female reproductive system was completely grossly diagnosed in order to know the infections in it. In addition, our study confirmed the importance of the ovaries and uterus in fertility of queens, The present study showed that six of the sample were uninfected in which the percentage was (75%); while two of the sample were infected in their ovaries and uterus in which the percentage was (25%). The result of the study showed that one of them has follicular cyst (12.5%) in ovary and endometritis (12.5%) .
Afficher plus [+] Moins [-]Evaluation the Effects of Administration Iraqi Dates Kernels Oil (sayer) and Malaysian Palm Kernel Oil on Some Blood Parameters in Pregnant and Lactated Female Rats Texte intégral
2022
Zainab Yaqoob | Bushra Hasan
The present study evaluates the effect of the extraction of Iraqi Date kernels oil (Sayer), and Malaysian palm oil on some. Haematological parameters during later gestation and lactation periods of female rats. The Iraqi palm kernel oil was collected and extracted using the Soxhlet, and the pure Malaysian kernel oil was purchased from Malaysia. The study was divided into two experiments; the first included 15 pregnant females at gestation days 17-19 divided into a first control group of late pregnancy and a second later pregnant administration with 150μL of IDKO, the third subgroup of later pregnancy administration with 150μL of MPKO. The second experiment, lactating was done on lactated mothers from one day of parturition until weaning day, divided into three subgroups: first control group second group lactated mother administration with groups compared with control groups. The parameters: RBC, Hb, PCV, platelets, and basophils increased significantly, and MCH, MCHC, MCV decreased significantly in IDKO and MPKO groups than control in the first experiment (17-19) GD. WBC count increased significantly in IDKO group than control and MPKO while lymphocytes decreased and neutrophils increased in MKPO group. The result from lactating study report that RBC count Hb, PCV, MCV, and MCH did not change significantly in all groups, while lymphocyte decreased. MCHC AND neutrophil increased IN IDKO and MPKO. In contrast, platelets and eosinophils increased significantly, and WBC s count and monocyte decreased in the IDKO group than control and MPKO group; WBCs and basophils elevated in MPKO more than in the control and IDKO. Conclusion: the administration of IDKO or MPKO supported and maintained hemopoietic tissue in pregnant and lactation states.
Afficher plus [+] Moins [-]Biomarkers for negative energy balance and fertility for early lactated dairy cows Texte intégral
2022
Umima Mansour | Howayda Belal | Ragab Dohreig
Most dairy cows suffered negative energy balance in early lactation that can affect their fertility .Adding fat to the cattle diet are used to increase the diet energy content . In this study level of ketone bodies (acetone and acetoacetate ) , nonestratefied fatty acids( NEFAs) and some individual fatty acids in serum and milk used as biomarkers for negative energy balance and fertility in dairy cows supplemented with two fat types. A comparison of calcium salts of palm oil fatty acid versus MixPromega-3 diet on the level of fatty acids in serum and milk of early lactating Holstein cows was valued and their relation to fertility. The diets were formulated to be isonitrogenous and isocaloric .The two diets given from day 0 to 30 days postpartum. Samples of blood and milk were collected at 1st,2nd and3rd weeks after calving. Results revealed that the increased serum and milk linoleic and linolineic acid with decreased saturated (palmitic and stearic ) fatty acids and oleic acid are related to improved energy status with decreasing the ketone bodies and nonestratefied fatty acids and enhance the fertility of dairy cattle fed Promixomega-3 when compared with calcium soap fed group . .Level of ketone bodies and nonestratefied fatty acids increased in milk than in serum in the same group. The fertility was enhanced by giving Promix omega -3 diet compared with calcium soap diet.
Afficher plus [+] Moins [-]Trial to control infectious bursal disease virus using iron oxide chitosan nanocomposite in broiler chicken Texte intégral
2022
Soad Nasef | Mervat Ayoub | Karim Selim | Dalia Elmasry
A severe outbreak of Infectious Bursal Disease (IBD) was recorded in 30 chicken flocks in different districts in Sharkia governorate during 2019, with high mortalities (up to 30%) and severe immunosuppression. Attempts to control IBD by an antiviral iron oxide chitosan nanocomposite was conducted. Eighty 21-day-old Baladi broiler chicks were divided into 4 groups. Group A; served as the negative control group, group B; was the challenge control group, group C; was the nanocomposite treatment group, and group D; received nanocomposite. The treatment contained 1 mg/ 100ml IC50 of iron oxide chitosan nanocomposite via the oral route administered for 3 days post-infection (PI). Results revealed that the iron oxide chitosan nanocomposite nano-size was 35.1±5nm with a stable state, zeta potentials16.8 ± 10.9, the PDI 0.91 and iron concentration in nanocomposite was 2625 mg/L. The virus shedding showed a significant decrease (P
Afficher plus [+] Moins [-]Anesthesia-induced severe esophagitis in a cat Texte intégral
2022
Öykü Sargın | Berkay Bakırel
This case report describes the development of severe esophagitis after an anesthesia event for a shaving procedure in a seven years old male cat. The cat was brought to our animal clinic with a complaint of severe vomiting and appetite loss. It was reported that vomiting has started after the administration of xylazine-ketamine anesthesia for the shaving procedure. In the endoscopic examination, severe esophagitis has been detected. Within two weeks, esophagitis was complicated with megaesophagus and then severe weight loss had been observed. Post-treatment endoscopic examination couldn't be performed because the esophagitis had developed after anesthesia. Regardless of whether a surgical procedure has been performed or the anesthesia has been performed repeatedly or once, post-anesthesia reflux esophagitis should also be suspected in cases of persistent appetite loss or dysphagia and vomiting after anesthesia.
Afficher plus [+] Moins [-]Assessment of soluble ST2 as a novel cardiac biomarker in cats with cardiomyopathy Texte intégral
2022
Yasemin KAYA | Utku BAKIREL
Soluble suppression of tumorigenesis-2 (sST2), a member of the interleukin-1 receptor family, is a novel biomarker of mechanical stress that is measurable in serum. It has been shown in humans and animals to be physiologically related to cardiac hypertrophy, fibrosis, and ventricular dysfunction. This study aimed to evaluate sST2 levels in cats with cardiomyopathy. In addition, serum sST2 and other cardiac biomarker levels were compared. Twenty-four client-owned cats with hypertrophic cardiomyopathy (HCM) and restrictive cardiomyopathy (RCM) were investigated. The cats included in the study were divided into four groups (I, II, III, and IV) according to the American College of Veterinary Internal Medicine (ACVIM) classification. Class B1 cats were included in group I (n=7), class B2 cats were included in group II (n=6), class C cats were included in group III (n=6), and healthy control-group cats in group IV (n=5). Measurement of cardiac troponin I (cTnI), cardiac troponin T (cTnT), N-terminal pro-brain natriuretic peptide (NT-proBNP), creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), sST2 levels, and echocardiographic examinations were performed in all groups, and correlations were investigated. We observed positive correlations among sST2 levels and CK-MB and LA diameter (p=0.05). There was no correlation between sST2, NT-proBNP, Troponin I, Troponin T, AST, and LDH levels. We confirmed positive correlations between NT-proBNP levels and LA diameter (p
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