To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campylobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Camplyobacter spp. were examined. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60 % of the isolates were noted to harbor plasmid DNA. Plasmid occurrence rate from pigs, chickens and cattle were 76.2 %, 61.7 % and 37.7 %, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10-1,000. Tetracycline resistant plasmids of C jejuni were transferarble to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E. coli and Vibrio cholerae. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation

The present study was conducted to investigate the incidence of Pasteruella multocida infection in Youngnam swine herds during the period from March 1988 to Ferbruary 1989 and some properties of the isolated organisms. P multocida was isolated from 22 (43.1 %) of 51 growing pigs of 4 to 12 weeks of age and from 8 (80.0 %) of 10 herds. From nasal turbinates of 102 slaughtered pigs, 47 (46.1 %) pigs were culture positive and pigs from 8 (88.9 %) of 9 heards were found to be infected with P multocida. From lungs of 101 slaughtered pigs, 42 (41.6 %) pigs were culture positive and the pigs from 11 (91.7 %) of 12 herds were found to be infected with P multocida. The majority of biochemical and cultural propertis of the P multocida isolates were identical to those of the standard strains. The isolation frequencies of P multocida in relation to pig snout lesion grades of 0 to 5 were 28.6 %, 41.6 %, 48.0 %, 50.0 % 85.7 %, and 100 %, respectively

In order to observe the pathological changes of kidney in rabbits infected with the viral haemorrhagic disease, the kidney tissues from the 91 rabbits infected with the viral haemorrhagic disease were examined by light and electron microscopy. On light microscopic observation, the kidney lesions were identified as haemorrhagic glomerular necrosis (33.0 %), membranous glomerulonephritis (20.9 %), thrombotic glomerulopathy (19.8 %) membranoproliferative glomerulonephritis (8.8 %), mesangial proliferative glomerulonephritis (8.8 %) ischemic acute tubular necrosis (7.7 %), and acute serous glomerulitis (6.6 %). On electron microscopic observation, cytoplasmic degeneration of mesangial cells, and irregular thickening of basement membranes with electron dense granular materials were observed. In podocytes swelling of mitochondria, dilatation of endoplasmic reticulum and extensive fusion of foot processes were also observed. Nonenveloped round icosahedral picornaviral particles with a diameter of 28-33nm were detected in the cytoplasm of degenerative endothelial cells, polymorphonuclear leucocytes, and monocytes

In vitro antifungal susceptibility test was carried out on 53 strains of Candida spp. isolated from milk of dairy cows with subclinical mastitis and teat cups of milking machines. Nystatin, clotrimazole, miconazole, econazole, 5-fluorocytosine, cycloheximide, haloprogin and griseofulvin were tested by the agar dilution method. The 84.8 % to 98.2 % of Candida strains were inhibited by clotrimazole, econazole and miconazole at = 20.32 micro g/ml)

The aim of the present study was to test the efficiency of estrous induction in the premature, metestrous and anestrous bitches. The estrus was induced with prostaglandin F2 alpha, estradiol-17 beta, pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) in the treatment A, and with PMSG and HCG in the treatment B. Day 0 was the first day of estrone injection in the treatment A and the day of PMSG injection in the treatment B. Twenty three of the twenty six bitches laparotomized under general anesthesia between 11 and 18 days after onset of behavioral estrus, whereas three bitches were not laparotomized and remained until parturition. Ovarian responses were evaluated with the total number of corpora lutea or ovulation sites. The uterine horns were flushed with phosphate-buffered saline added heat treated canine serum (10 %), the flushing media was collected into watch glass and the ova were examined under stereomicroscope. Standing estrus was observed on the day 17.7 +- 1.5 after injection of estrone in the treatment A, but ovarian responses were not detectable. Standing estrus was observed on the day 12.2 +- 0.2 after injection of PMSG in the treatment B and 14 of 17 bitches showed ovarian responses. Ova were recovered in 9 of the 14 bitches. Ovarian responses were observed in one of the three premature bitches, two of the three metestrous bitches and all of the 11 anestrous bitches. The average number of the ova collected from 9 bitchs were 12.2 +- 1.4. Three bitches in the treatment B exhibited behavioural estrus and all of them were mated with fertile male dog, resulting the pregnancy in only one bitch. The pregnant bitch gave the birth of two pups

This study was designed in order to clarify the effect of ethanol drinking on the mineral contents on liver, kidney, muscle and hair. Forty-five rats were divided into 2 groups and a control group. The control group received tap water and the other 2 groups were given 8 % and 40 % ethanol as drinking source. Liver, kindney, muscle and hair samples were taken and analyzed for zinc, calcium and copper contents by atomic absorption spectrophotometric methods. The zinc content of muscle showed significant (P0.01) decrease in both groups. The calcium content of hair showed significant (P0.1) increase in 8 % ethanol group. The copper contents of kidney and muscle in 8 % ethanol group and liver in 40 % ethanol group showed significant (P0.1) decrease

The etiologic agents of mucormycosis are several nonpathogenic fungi of the order Mucorales and are ubiquitous. A total of 82 chickens were infected with the disease and developed anorexia, diarrhea, malnutrition, dyspnea, and paralysis in a chicken farm, Sokcho, Kwangwon. At necropsy there were multiple nodular lesions and hemorrhages in livers, spleens, kidneys, gastro intestinal track, and respiratory system. On histopathological examination it was found that the nodular lesions were consisted of granulomatous inflammation accompanying characteristic hyphae (4-24 micro wide) of the fungi

Nine strains of Yersinia species isolated from pigs and dogs in Korea, comprising 5 strains of Yersinia enterocolitica, 2 strains of Y. kristensenii and each strain of Y. pseudo-tuberculosis and Y. intermedia, were examined for the presence of virulence-associated plasmids, calcium dependency and provocation of guinea pig conjunctivitis (Sereny test). Three strains of Y. enterocolitica isolated from pigs were positive in calcium dependency and harbored one plasmid of about 45 megadalton, but negative in Sereny test

The effects of the serum total protein and immunoglobulin levels of the colostrum-conferred Korean native calves at 2 days of age on the disease incidence during the suckling period were studied. Serum total protein, total immunoglobulin, IgG, and IgM levels of 6 Korean native calves died from septicemia were lower than those of calves survived (p0.05). Korean native calves having low serum total protein and immunoglobulins were affected with diarrhea earlier in life, when compared with calves having high serum levels (P0.05). Korea native calves having low serum total protein were affected with bronchopneumonia earlier in life, when compared with calves having high serum levels (P0.05). Korean native calves having low serum neutralizing antibody titers to bovine viral diarrhea virus had high incidence rate of BVD, when compared with calves having having high serum neutralizing antibody titers (P0.05)

In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2aminoethyl-isothiouronium bromide (AET) and dextran (Dex), singly or in combination. To further standardize the assay, optimum concentration of AET-and/or Dex-treatment and incubation time for rosette forming cell (RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythrocyte-antibody (EAfc)- and erythrocyte-antibody-complement (EAC)- rosetting techniques. The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette (6.7 +- 2.4 %) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8 % Dex were formed as 37.3 +- 2.7 % and 45.1 +- 2.1 %, respectively. And the synergistic effects were noted no less than 66.5 +- 5.6 % when the KGRBC treated with 0.1M AET and 8 % Dex subsequently and rate of RFR did not change significantly between 3-24 hours incubation time at 4deg C, EA-and EAC-RFR were 23.3 +- 9.1 % and 23.1 +- 7.9 %, respectively. These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA-or EAC-rosette assay in cattle-PBL