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Clinical Evaluation of The Use of PRP as A Treatment for Achilles Tendon Defects in Dog Model Texte intégral
2024
hajar Nisheemi | ashraf. abdulrazaq | Hussain aljabery
The Achilles tendon is the most resilient tendon in the musculoskeletal system of canines. It enables the hock to extend, allowing the animal to balance on its toes. Its primary role is to propel the rear limb forward and provide support to the hock. Platelet-rich plasma (PRP), Platelets store growth factors and are crucial in various physiological functions like blood clotting, blood vessel formation, immune defense, and tissue healing. Aim of the study: To analyze and contrast the impacts of Platelet-rich plasma on regeneration of Achilles tendon. Materials and Methods: 10 dogs were divided into two groups: 5 dogs received treatment with Platelet-Rich Plasma (Group A), 5 dogs served as a control (Group B), and left untreated Results: scoring the degree of lameness in dogs that were not treated throughout the entire experiment The control group showed lameness scores, resulting in the animals being unable to lift their legs and take a few steps,The group that was treated with PRP had notably lower levels of lameness and pain throughout the study period,prp treatment resulted in better healing of the injured tendon compared to the untreated group, showing gradual improvement after 7 days. Histologically,PRP enhanced healing with increased cell proliferation, migration and collagen production of tendon-derived cells including increased cellularity and vascularity of ligament tissue ,compared to those in the control group the of the Achilles tendon of the positive control group showed chronic tendonitis. Conclusion: Proved that PRP can greatly enhance tendon repair, tendon growth, and minimize inflammation.
Afficher plus [+] Moins [-]Evaluation the Effect of Stevia Leaves Powder on Antioxidant, Digestive Enzyme and Meat Quality in Broiler Chickens Texte intégral
2024
Zainab Ibrahim | Nameer Khudhair | Measem Ali
commercial poultry farming is a rapidly growing sector. therefore, the study Aimed to evaluate the impact of stevia leaf powder on oxidative status and digestion and their reflection on meat quality parameters in broiler Chickens.Materials and methods From December 21, 2023, to January 17, 2024, 240 Ross broiler chicks were given different Stevia leaf powder dosages. Four groups of Babylon's Modern Al-Bakri Hatchery chicks received different treatments for 28 days. Group 1 (control) got a conventional diet, while Groups 2, 3, and 4 received diets supplemented with 10 g/kg, 20 g/kg, and 30 g/kg Stevia leaf powder. Locally ground stevia was added to the diet. The study examined digestive enzyme levels, antioxidant activity, and meat quality in broiler chickens treated with varied Stevia leaf powder (STV) concentrations. The STV-treated groups had significantly higher amylase levels (p≤ 0.05) than the control group, with no significant variations across STV concentrations. The 30g/kg STV group had slightly increase in lipase levels, but otherwise no changes. Compared to other groups, the 20g/kg STV group had considerably greater antioxidant enzyme activity, including SOD, CAT, and GLUpx. Chickens treated with 10g/kg had higher antioxidant activity, but the group did not vary from control. STV supplementation reduced meat pH, which improves tenderness and shelf life, and slightly increased cooking loss. The 20g/kg STV group showed decreased drip loss, indicating greater water retention and meat quality.
Afficher plus [+] Moins [-]Clinicopathological Changes Associated with Bovine Babesiosis and Trypanosomiasis in Cattle Texte intégral
2024
Israa Al-Robaiee | Maab Al-Farwachi
Clinicopathological changes associated with blood protozoa in cows have been well-examined. However, these changes can be influenced over time due to the development of the animal immune system status and environmental changes. The current study aimed to assess clinical and pathological changes associated with blood protozoa infection in naturally infected cattle. A total of 80 cows were examined for evidence of babesiosis and trypanosomiasis using Giemsa-stained blood smears. Hematological parameter, clotting factor, and urinalysis were also estimated. Babesiosis and trypanosomasis were identified in 16% and 33% of the examined stained blood smears, respectively. The study revealed a significant decrease (p<0.05) in values of hemoglobin concentration, packed cell volume, total erythrocytes count, mean corpuscular hemoglobin, and total platelet count in both infected groups with babesiosis and Trypanosomiasis . In contrast, a significant increase was recorded in total leukocytes count, clotting time, prothrombin time, fibrinogen time, and activated partial thromboplastin time, as well as platelet volume and platelet distribution width in the infected cattle compared with non-infected cattle. Urinalysis showed appearance of urobilinogen, bilirubin, protein and glucose in the urine of some infected animals, while nitrate and ketones were not detected in all study animals. We concluded that hemoprtozoa revealed an obvious effect clotting system which might reflected disseminated intravascular coagulation (DIC) abnormalities and changes in urine components Therefore, clinicopathological changes associated with babesiosis and trypanosomiasis should be evaluated periodically to evaluated animal health status.
Afficher plus [+] Moins [-]Atypical loculated pericardial effusion with infectious pericarditis mimicking a cardiac mass in a dog: a case report Texte intégral
2024
Lee, J.Y. | Bae, S.G. | Park, J.K. | Jang, M. | Lee, K.J. | Lee, S.K.
A 13-year-old, Maltese dog presented with syncope and lethargy. Abdominal ultrasonography demonstrated anechoic peritoneal effusion and hepatic congestion. A focal echogenic round mass compressing the right ventricle and atrium was observed on echocardiography. Cardiac tamponade and right ventricular outflow tract obstruction occurred. On computed tomography, a homogeneous soft-tissue structure compressing the right chamber without contrast enhancement, suspected to be loculated pericardial effusion. During pericardiocentesis, cardiac tamponade was resolved, and irregular pericardial thickening was noted. Pericardial effusion was exudate and gram-positive bacterial colonies were observed on cytology. A diagnosis of fibrinous pericarditis secondary to bacterial infection was established.
Afficher plus [+] Moins [-]Development of a blocking ELISA for detection of Japanese encephalitis virus antibodies in pig and horse sera Texte intégral
2024
Yang, D.K. | Kim, E.J. | Jang, S.H. | Lee, H.J. | Kim, B.N. | Lee, J.A. | Lee, J.Y. | Cho, Y.S.
Japanese encephalitis virus (JEV) is a mosquito-borne virus that can infect pigs, horses, and other mammals, including humans. Sero-epidemiological investigations of JEV have been performed using hemagglutination inhibition (HI), virus neutralization (VN) tests and enzyme-linked immunosorbent assay (ELISA). A need exists for a new ELISA that can detect JEV antibodies in the sera of several animal species. We aimed to develop a blocking ELISA (B-ELISA) for detecting JEV antibodies in pig and horse serum samples. JEV antibodies in 218 pig and 315 horse serum samples were measured using HI and VN tests. The purified KV1899-306 strain was used as an antigen for B-ELISA. The purified antibody (7A13) was conjugated with horseradish peroxidase and used as a detector antibody. The sera of pigs and horses to measure antibody against JEV were subjected to B-ELISA and analyzed. The B-ELISA had a diagnostic sensitivity of 94.6% to 100%, a specificity of 91.2 to 100%, and an accuracy of 94.9 to 98.6% compared with those of the HI and VN tests in pig and horse sera. The B-ELISA had a higher correlation with pig sera (r = 0.89 and 0.90 for VN and HI) than with horse sera (r = 0.75 and to 0.79). The new B-ELISA could be useful in the sero-surveillance of JEV in pig and horse sera and replace indirect ELISA.
Afficher plus [+] Moins [-]Grain-free diet-induced dilated cardiomyopathy with atrial fibrillation in a Labrador Retriever: a case report Texte intégral
2024
Baek, M.J. | Choi, M.S. | Chae, Y. | Yun, T.S. | Kang, B.T. | Kim, H.H.
We describe the first reported case of grain-free diet-induced dilated cardiomyopathy (DCM) in a dog in Korea. An 11-year-old female dog was referred with abdominal distention, anorexia, and vomiting, having been fed a grain-free diet for more than 5 years. Thoracic radiography revealed cardiomegaly and pulmonary edema. Atrial fibrillation was detected using electrocardiography. The dog was tentatively diagnosed with congestive heart failure (CHF) secondary to grain-free diet-induced DCM, and its diet changed to contain grain. Digoxin and diltiazem were prescribed for the atrial fibrillation, and pimobendan, enalapril, and furosemide for CHF. Significant improvements in echocardiographic indices were confirmed after 3 months.
Afficher plus [+] Moins [-]Comparison of academic achievement, graduation competency and academic sustainability of veterinary students based on college admission processes Texte intégral
2024
Nahm, S.S. | Kim, Y.J. | Yang, S.K.
A university’s admission policy is established to select applicants who can successfully complete the curriculum. Thus, it is important to develop optimal admissions processes for applicant selection. This study was conducted to infer the most suitable admission processes for veterinary school applicants by examining academic achievement, graduation competency and academic sustainability. It was conducted on students admitted from 2009 to 2018 at one of the veterinary colleges in Korea. The admission methods were divided into, first the regular admission (RA) process, primarily reflecting the results of the College Scholastic Ability Test, and second, the rolling admission processes. The rolling processes were further divided into admission officer-based (AO), high school grade point average-based (GA), essay testbased (ET), admission for the underprivileged (UP) and admission for overseas Koreans and foreigners (OKF). The final grade point average was observed to be higher in the order of the GA, AO and RA groups. The survey on the veterinary graduation competency did not reveal any difference between the regular and rolling admission processes. However, among the latter, AO-based admission showed a higher ratio of outstanding competency levels. In terms of academic sustainability, OKF admission demonstrated a higher rate of failure while RA showed a higher rate of expulsion and dropout. Summarizing the above results, the most successful veterinary applicants were selected through AO-based admission. These results are meaningful in providing the basic information required to establish admission policies for veterinary colleges in Korea.
Afficher plus [+] Moins [-]Catechin reduces liver inflammation by regulating Kupffer cell activation in rats Texte intégral
2024
Ahn, M.J.
The liver is a defense against infections due to its strategic location between the gastrointestinal and systemic circulations. In dogs and cats, infectious hepatitis encompasses a range of contagious diseases affecting the liver either directly or as part of a broader systemic infection, including bacterial, mycobacterial, viral, fungal, protozoal, parasitic, and rickettsial diseases. Catechins possess well-recognized natural antioxidant properties. This study investigated their therapeutic potential for applications in hepatology, evaluating whether catechins reduce hepatic inflammation in rats repeatedly exposed to carbon tetrachloride (CCl₄). Sprague-Dawley rats were given catechin 50 (C50) or 100 (C100) mg/kg body weight orally daily for 3 days. This treatment was given with or without concurrent intraperitoneal injections of CCl₄. Phosphate-buffered saline served as the vehicle control, while silymarin administered at 100 mg/kg was used as the positive control. Gross examination revealed significant enlargement, edema, and darker tissue in CCl₄-induced livers treated with vehicle. Additionally, spotty discoloration was observed on the surface. Kupffer cell activation suppressed the expression of inflammatory mediators, including inducible nitric oxide synthase (iNOS), in groups co-treated with catechin and CCl₄; this effect was reversed when catalase replaced catechin in CCl₄-injured rats. Catechin alleviates hepatic inflammation in rats repeatedly exposed to CCl₄; it also modulates the activation of Kupffer cells and monocytes. These results should lead to new treatments for liver inflammation in veterinary practice.
Afficher plus [+] Moins [-]Are there laterality differences in passive flexion and extension of the proximal limb joints in working Siberian Husky dogs? Texte intégral
2024
Soper, S. | Charlton, S. | Hunnisett, A.
Differences between left and right-side joint range of motion may affect canine locomotive ability and movement. Passive range of motion (PROM) joint measurement provides the limits that a particular joint can move in its physiological planes of motion without influence of muscle activity. To compare left and right-side flexion and extension of the glenohumeral, humeroulnar/humeroradial, coxofemoral and femorotibial joints and for laterality PROM differences. Siberian Husky dogs were selected (n = 18), mixed gender, aged (1.4–11.8) years living and working together. Goniometry measured joint PROM, a validated, non-invasive method. Dogs were conscious and placed in standing position. Triplicate measures of joint flexion and extension were taken bilaterally of each dog for afore-mentioned joints. Median values of triplicate measures were computed. Paired t-tests compared laterality of joint PROM, gender, age (< 6 vs. ≥ 6 years) effects. Inferential symmetry indices [SI] were calculated. For all joints, there was no significant difference (p > 0.05) between left and right-side flexion and extension measures nor between genders. Age (< 6 vs. ≥ 6 years) had a significant effect on right hip flexion (p < 0.001); both left and right-side shoulder flexion (p < 0.001); elbow flexion (p = 0.001 and p < 0.001); hip extension (p = 0.02 and p < 0.001) respectively. The shoulder joint showed greatest PROM asymmetry (SI = 3.63%). Bilateral PROM measures are important to consider in joint movement and assessment. These results warrant further investigation with larger cohorts of defined age groups.
Afficher plus [+] Moins [-]Osteological analysis of Nyctereutes procyonoides bones excavated from the Gasan-ri archaeological site Texte intégral
2024
Lee, S.J. | Choi, J.H. | Won, C.K.
Archaeological research has mainly focused on stone tools, pottery, and metal relics excavated from ruins. Recently, interest in natural relics such as animal bones has increased, as these relics can provide insight into the eating habits, cultural level, and perception of animals of the people at that time. This study investigated morphological structures and dimensions of Nyctereutes procyonoides bones excavated from a well at the Gasan-ri archaeological site in Jinju. These bones are presumed to be relics from the Three Kingdoms period. A total of 447 animal bones were excavated, of which 59 (13.2%) were identified as N. procyonoides bones. These identified bones weighed 47.14 g, accounting for 4.83% of the total bone weight (975.30 g). These N. procyonoides bones were found to belong to 2 individuals estimated to be 5 to 6 weeks old. These bones included 5 skull fragments, 2 mandibles, 9 vertebrae, 11 ribs, 10 forelimb bones, 3 hip bones, and 19 hindlimb bones. A fracture was observed near the bregma of one skull. The presence of a complete specimen in the well suggested that it was used in animal sacrifice rituals. This study estimated the value of animals and human dietary culture at that time by utilizing N. procyonoides bones presumed to be relics of the Three Kingdoms period.
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