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Use of ELISA for detection of immunoglobulins G and M that recognize Salmonella dublin lipopolysaccharide for prediction of carrier status in cattle.
1990
Spier S.J. | Smith B.P. | Tyler J.W. | Cullor J.S. | Dilling G.W. | Pfaff L.D.
Immunoglobulin reactions to Salmonella dublin in serum and milk from 4 groups of lactating cows were measured by an indirect ELISA. The groups consisted of (1) cows that were natural carriers of S dublin in the mammary gland, (2) experimentally infected cows that did not become carriers, (3) cows inoculated with a commercial S dublin bacterin, and (4) cows used as S dublin-negative controls. Milk and serum samples were obtained at monthly intervals. Models for predicting carrier status were developed by use of stepwise logistic regression. Independent variables consisted of serum and milk IgG and IgM titers to S dublin lipopolysaccharide and a ratio of IgG to IgM. The utility of a single sample vs multiple samples obtained at 1-month or 2-month intervals was tested by comparison of goodness-of-fit X2 P values for 8 models predicting carrier status. Immunoglobulin reactions specific to S dublin were a significant predictor of carrier status (P < 0.001). Serum IgG titers specific for S dublin were the most important variable for predicting carrier status. Two serum IgG titers to S dublin obtained 2 months apart was a better predictor of carrier status than measurement of the IgG:IgM ratio from a single serum sample. Immunoglobulin recognizing S dublin epitopes also were detected in milk samples. In milk, performing 2 ELISA 60 days apart to determine IgG and IgM reactions to S dublin appeared to be useful for the prediction of carrier status, but was not as accurate as models for serum immunoglobulin reactions.
Afficher plus [+] Moins [-]Fine structure of Theileria sergenti merozoite in Korean native cattle.
1990
Baek B.K. | Kim B.S. | Lee H.I.
Light microscopic observations on the in vitro effects of praziquantel on Heterophyopsis continua.
1990
Woo H.C. | Suh M.D. | Hong S.J.
Avidin-biotin complex for immunohistochemical diagnosis of Aujeszky's disease and hog cholera.
1990
Kim S.B. | Sur J.H. | Moon U.G.
Pathogenicity, hemagglutinability and the effect of physicochemical agents on virus of rabbit hemorrhagic disease.
1990
Yoon I.J. | Jeon Y.S.
Immunohistochemical identification of Newcastle disease virus with indirect immunoperoxidase technique.
1990
Nho W.G. | Sur J.H. | Kim S.B.
Studies on the early diagnosis of pregnancy of dairy cows by EIA-kit of progesterone in milk.
1990
Kim M.K. | Shin H.J. | Lee M.H. | Lee M.H. | Kim S.K.
Effects of ammonia water on sporulation of coccidial oocysts originated from bovine.
1990
Wee S.H. | Kang Y.B. | Jang H. | Lee H.S. | Choi S.H.
Antagonism of xylazine-induced hypotensive effect by yohimbine in rabbits.
1990
Shin D.H.
Cultivo de Spirulina maxima ao ar livre. 1: inverno.
1990
Ruiz R.L. | Mos E.N. | Lima C.G. de | Ribeiro M.A.M.
