Objective: In this work, the effect of probiotics on the state of the microbial background of the livestock building, on the state of udder teats, and on the prevalence of latent mastitis was investi¬gated. Long-term use of the consortium has bactericidal activity against all cultures studied, especially the causative agent of mastitis, Staphylococcus aureus, except Proteus mirabilis, Proteus vulgaris, and Escherichia coli. Materials and Methods: The washes from animal housings and milk samples were collected from the dairy farms "Astana-Onim" Joint Stock Company and "Rodina" Limited Liability Partnership (Kazakhstan). The cleaning solutions and probiotic agents were applied directly to the udder teats of cows before and after milking. Diagnosis of subclinical mastitis was performed using the Kenotest rapid mastitis test. Directly counting the number of somatic cells in the collected milk samples from each cow was performed on a somatic cell counter. Results: Pathogenic microorganisms, including S. aureus bacteria (50% of samples) and bacteria of the E. coli group, Enterobacter aerogenes, and P. mirabilis (36% of samples), were detected on the udder skin and milk wipes. Using a consortium of probiotic microorganisms positively affects the mammary gland more quickly than using mastitis prevention agents alone. Probiotic use for a month resulted in a significant improvement in udder teat condition, with 60.7% of teats showing normal physiological reaction to milking, a decrease in complicated hyperkeratosis, and an increase in uncomplicated mastitis. The studies showed that in the experimental group, there was a 1.5-fold reduction in the number of cows with clinically pronounced mastitis. The experimental group showed no significant changes in the number of animals with high somatic cell levels before and after the study, while the control group without probiotics had a significant increase in dis¬eased animals after 1 month. Conclusion: The use of a probiotic consortium has shown promising results in reducing the inci¬dence of mastitis and improving milk quality in cows. [J Adv Vet Anim Res 2023; 10(2.000): 185-195]

Objectives: Ducks suffer a huge economic loss as a result of infections with Pasteurella multocida and Riemerella anatipestifer, which cause high morbidity and mortality. Because these patho¬gens induce similar clinical symptoms when coinfections occur, it is very difficult to differentiate between them based just on clinical signs. Hence, these major pathogens must be quickly and accurately detected. Materials and Methods: A total of 104 birds ranging from 2 days to 4 weeks old were collected from Egyptian farms, and the outcomes were compared statistically. Conventional cultural iden¬tification procedures and a direct multiplex polymerase chain reaction assay were utilized to recognize both pathogens in a single tube reaction simultaneously. Then, the obtained isolates were characterized phenotypically and genotypically. Results: Clinical signs appear at 2–4 weeks of age with respiratory distress (dyspnea), white fluid feces, and stunting. The scrutinized data demonstrated a significantly higher detection rate by PCR directly compared to classical culture procedures. Pasteurella multocida was detected only by PCR. The disc diffusion technique against ten antibiotics showed absolute susceptibilities to amik¬acin, doxycycline, and florfenicol. High levels of beta-lactam resistance were observed. Riemerella anatipestifer isolates were screened for pathogenicity and plasmid-borne blaTEM genes. All six isolates harbored five virulence genes: aspC, RA46, m28, pstS, and Nlp/P60. Moreover, blaTEM was identified into four isolates and deposited to GenBank with accession numbers OP347083, OP347084, OP347085, and OP347086. Conclusion: These results suggest advanced PCR assays can be applied to the field for rapid and valuable diagnosis of two significant pathogens and focus on the worth of ducks in the propaga¬tion of transferable antibiotic resistance genes into the environment. [J Adv Vet Anim Res 2023; 10(2.000): 211-221]

Objective: The study investigated the gut content and recorded morphometric and hematological parameters in the Eurasian collared dove (Streptopelia decaocto). Materials and Methods: 24 samples of healthy birds (12 from each sex) were collected from different wetlands in Punjab, Pakistan, from December 2022 until February 2023. Birds were cap¬tured live for blood samples, morphometric, and gut analyses. Results: The current study revealed that mensural measurements showed no significant differences in all parameters except tail length, which was significantly longer in males (14.59 ± 0.30) compared to females (12.88 ± 0.43). Hematological parameters were hemoglobin, 23.95 gm/dl; red blood cells, 3.97 × 106/μl; white blood cells, 429.9.67 × 103/μl; hematocrit, 72.14%; mean cor¬puscular volume, 183.24 FL; mean corpuscular hemoglobin, 61.70 pg; mean corpuscular hemo¬globin concentration, 32.37 pg; platelets, 7.01/μl; and red cell distribution width, 110.86/μl. The percentages of neutrophils, lymphocytes, monocytes, and eosinophils were 71.33%, 23.03%, 3.30%, and 1.43%, respectively. The gut content of the Eurasian collared dove mainly consisted of rice, wheat, corn, and millet seeds. Some stony materials were also present. Conclusion: Our study concluded that male and female Eurasian collared doves are alike in biometrics (except tail length) and hematological profiles. Gut content and weight were also similar. Males were slightly larger than females. The gut content showed that the Eurasian collared dove mainly feeds on rice, wheat, corn, and millet seeds. In this study, hematological parameters were also studied. [J Adv Vet Anim Res 2023; 10(2.000): 244-248]

Objective: Various disease complications are a risk of overweight or obesity, so losing weight can reduce the risk of diseases caused by obesity. Binahong leaf ethanol extract (Anredera cordifolia) is a weight-loss herbal preparation. Aim: This study aims to analyze whether A. cordifolia extract is effective in losing weight by affecting the mechanism of adipogenesis in an animal obesity model. Materials and Methods: Animals were grouped into six groups as follows: the normal diet (K1), the negative control group (K2), the positive control group with Orlistat at a dose of 20 mg/kg BW (K3), an ethanol extract of A. cordifolia leaves at doses of 50 mg/kg BW (P1), 100 mg/kg BW group (P2), and 150 mg/kg BW (P3). All rats were fed a diet that consisted of high fat for eight weeks, except K1. Afterward, the treatments were given based on group distribution. Then, the rats were treated based on their groups for 4 weeks, and the high-fat diet was still given during the treatment for the control groups (K2). Anthropometric examinations such as body weight, length, and the circumference of the abdomen were measured. Metabolic parameters, including blood glucose, cholesterol levels, triglyceride levels, and abdominal fat weight, were measured using molecular parameters that measured PI3K levels and Extracellular signal-regulated kinase (ERK) in abdominal fat tissue samples using the ELISA method. Results: ERK levels of abdominal fat were lowered in the treatment group using the extract of A. cordifolia (50 mg/kg BW (P1) and 100 mg/kg BW (P2)) compared to the control group that was given a high-fat diet without treatment. The control group, which was fed a high-fat diet without treatment, had an average ERK level of 10.17 ± 2.98 ng/ml, P1 (50 mg/kg BW). Furthermore, when ethanol extracts were used as opposed to the control group, which received a high-fat diet without treatment, there was an increase in phosphoinositide three-kinase (PI3K) levels (K2). The control group received 9.35 ± 2.87 ng/ml, the treatment group received 100 mg/kg BW (P2) 9.48 ± 1.54 ng/ml, and the treatment group received 150 mg/kg BW (P3) 7.87 ± 1.79 ng/ml. The weight of fat in the abdomen differed between the groups that received a high-fat diet without treatment (K2) and those that received a high-fat diet with treatment (P1, P2, P3; p < 0.05). Conclusion: Anredera cordifolia extract possesses anti-obesity activities by decreasing ERK and increasing PI3K levels, as well as reducing abdominal fat weight. [J Adv Vet Anim Res 2023; 10(4.000): 809-816]

Objective: This study aims to evaluate the effect of Clostridium perfringens sialidase treatment on monolayer cell behavior using computational screening and an in vitro approach to demonstrate interaction between enzyme-based drugs and ligands in host cells. Materials and Methods: The in silico study was carried out by molecular docking analysis used to predict the interactions between atoms that occur, followed by genetic characterization of sialidase from a wild isolate. Sialidase, which has undergone further production and purification processes exposed to chicken embryonic fibroblast cell culture, and observations-based structural morphology of cells compared between treated cells and normal cells without treatment. Results: Based on an in silico study, C. perfringens sialidase has an excellent binding affinity with Neu5Acα (2.3) Gal ligand receptor with Gibbs energy value (ΔG)—7.35 kcal/mol and Ki value of 4.11 μM. Wild C. perfringens isolates in this study have 99.1%–100% similarity to the plc gene, NanH, and NanI genes, while NanJ shows 93.18% similarity compared to the reference isolate from GenBank. Sialidase at 750 and 150 mU may impact the viability, cell count, and cell behavior structure of fibroblast cells by significantly increasing the empty area and perimeter of chicken embryo fibroblast (CEF) cells, while at 30 mU sialidase shows no significant difference compared with mock control. Conclusion: Sialidase-derived C. perfringens has the capacity to compete with viral molecules for attachment to host sialic acid based on in silico analysis. However, sialidase treatment has an impact on monolayer cell fibroblasts given exposure to high doses. [J Adv Vet Anim Res 2023; 10(4.000): 667-676]

Objective: The results of G1 and G4 polymorphisms as litter-size (LS) markers of ewes remain contradictory. The aim was to evaluate the impact of G1 (c.260 G>A) and G4 (c.721 G>A) polymorphisms on the LS of sheep by synthesizing data from multiple previous studies. Methods: Data were extracted from 14 eligible articles. The genotypes of G1 and G4 polymorphisms were homozygous wild-type (WW), heterozygous (WM), and homozygous mutanttype (MM). The standardized mean difference (SMD) method using random effect models was employed to determine the effect size of G1 and G4 polymorphisms on LS under dominant, recessive, additive, and co-dominant genetic models. Heterogeneity was analyzed with the I2 statistic index. Publication bias was depicted with funnel plots and tested by Egger's and Begg's tests. Results: The study showed that the correlation between G1 polymorphism and LS in sheep was not significant (p > 0.05) under all genetic models. The influence of G4 polymorphism on the LS of sheep was found significantly (p < 0.05) under dominant [SMD = 0.28, I2 = 0% (no heterogeneity)] and co-dominant [SMD = −0.14, I2 = 36% (moderate heterogeneity)] genetic models. The WM genotype of G4 polymorphism increased LS, while the MM genotype reduced LS in sheep. Publication bias among G1 and G4 polymorphism studies was absent in all genetic models. Conclusion: Thus, the study revealed that G4 polymorphism could be a potential genetic marker for LS in ewes. On the contrary, G1 polymorphism has no association with the LS of ewes. [J Adv Vet Anim Res 2023; 10(4.000): 599-607]

Objective: The main goal of the study was to find whether pumpkin (Cucurbita pepo) extract has any preventive or antioxidant properties against acute uranyl acetate (UA)-induced reproductive cytotoxicity. Material and Methods: Four groups each, including 10 adult male rats, were randomly assigned. (GI): the control group was given 1 ml of purified water orally for 30 days. (GII): Rats were given orally, a single dose of 150 mg/kg b.w. UA (GIII): Rats consumed 40 mg/kg b.w. of pumpkin seed extract (PSE) orally every day for 30 days. (GIV): Rats received a single dose (150 mg/kg b.w.) of UA plus a daily oral dose of PSE (40 mg/kg b.w.) for 30 days. Animal sacrifice was used for oxidative stress and histopathological study. Results: Showed significantly (p ≥ 0.001) elevated malondialdehyde levels in the GII group (6.19 ± 0.4), while GIII and GIV showed no significant differences. Glutathione peroxidase showed a significant (p ≥ 0.001) decrease (2.55 ± 0.2) in the GII group, while in groups (GIII and GIV), it showed a significant (p ≥ 0.001) increase (4.61 ± 0.16, 4.28 ± 0.032), respectively. The histopathological study for GII groups showed sloughing of epithelial cells lining the seminiferous tubules with a decrease in the number of spermatozoa in some tubules. Many sections revealed hyperplasia of the epithelial cells lining the seminiferous tubules with necrosis. The GIII and GIV groups showed normal histological structures with an increase in spermatogenesis in the testes and epididymis tissues. Conclusion: We concluded that UA causes oxidative stress and histopathological alterations in the rat reproductive system. Pumpkin extract plays a role in improving the activity of the reproductive system. [J Adv Vet Anim Res 2023; 10(4.000): 647-653]

Objective: This research aims to investigate the microbial diversity of Budu prepared from fresh and frozen fish from the Pariaman and Pasaman districts in West Sumatra Province, Indonesia, as well as provide basic information about Budu quality. Materials and Methods: To obtain the bacterial microbial composition, deoxyribonucleic acid extraction was carried out using amplicon-sequencing of the 16S-rRNA gene in the V3–V4 region from two types of Budu and carried out in duplicate. Results: Budu prepared with fresh (Pariaman) or frozen (Pasaman) fish was dominated by Firmicutes (78.455%–92.37%) and Proteobacteria (6.477%–7.23%) phyla. The total microbial species in Budu from Pariaman were higher (227 species) than in Pasaman (153 species). The bacterial species found are Lentibacillus kimchi (1.878%–2.21%), Staphylococcus cohnii (0.597%–0.70%), Peptostreptococcus russeli (0.00%–0.002%), Clostridium disporicum (0.073%–0.09%), Clostridium novyi (0.00%–0.01%), Nioella sediminis (0.00%–0.001%), and Shewanella baltica (0.00%–0.003%). Lentibacillus kimchi, S. cohnii, and C. disporicum are found in both Budu. Nioella sediminis and S. baltica are found in Budu Pariaman. Peptostreptococcus russeli and C. novyi were found in Budu Pasaman. Conclusion: Metagenomic analysis of Budu from different fish, Pariaman (fresh fish) and Pasaman (frozen fish) showed that the biodiversity of bacteria was barely different. Both Budu found lactic acid bacteria from the Enterococcaceae family, genus Vagococcus, and pathogenic bacteria, such as S. cohnii, P. russeli, C. disporicum, and S. baltica. The discovery of various species of pathogenic bacteria indicates that development is still needed in the Budu production process to improve Budu quality. [J Adv Vet Anim Res 2023; 10(4.000): 801-808]

Objective: Dementia is a common aging-related neurodegenerative disease in the elderly worldwide. Alterations in neurogenesis and angiogenesis factors have been linked to cognitive impairment in neurological disorders. However, synthetic drugs to improve memory disorders have uncomfortable side effects. The purpose of this study is to explore the neuroprotective potential of the fruit ethanol extract of andaliman (Zanthoxylum acanthopodium DC) [Andaliman fruit ethanol extract (AEE)] on brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), and spatial memory in rat models of aging. Materials and Methods: This study had an experimental design with AEE. The 4 groups were treated as follows: N (normal), M (served as positive control), P1 (AEE 150 mg/kg bw), and P2 (AEE 300 mg/kg BW) for 8 weeks. Aged model rats (M, P1, and P2) were obtained by inducing D-galactose (150 mg/kg bw). BDNF and VEGF expression were determined by RT-PCR, and spatial memory was assessed using the test of the Moris Water Maze (MWM). The Kruskal–Wallis and Mann–Whitney tests were used to assess the statistical analysis. Results: AEE had a tendency to increase BDNF in P2 compared to the normal group (1.98 versus 1). VEGF expression increased in P1 and P2 compared to the normal group (1.14 and 1.29 versus 1). AEE at a dose of 300 mg/kg bw significantly improved spatial memory (p = 0.026). Conclusion: For eight weeks, AEE at a dose of 300 mg/kg bw considerably increased the potential to enhance VEGF and BDNF expression as well as spatial memory. [J Adv Vet Anim Res 2023; 10(4.000): 587-592]

Objective: The study aimed to determine the effect of giving concentrate protein from closed-house (CP-CH) broiler farm waste in rations on the meat quality (physical-chemical) and blood profile of landrace crossbreed pigs. Materials and Methods: The study used a completely randomized design (CRD) with 3 treat¬ments and 4 replications with 12 finisher-phase landrace pigs with an average body weight of 63.42 ± 2.39 kg. The treatments were: A (control): use of 0% CP-CH + 24% CP-152 concentrate; B: 12% CP-CH + 12% CP-152 concentrate; and C: 24% CP-CH + 0% CP-152 concentrate. The research parameters were meat quality (physical-chemical meat) and blood lipid profile [total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TGs)]. Results: The results showed that treatment group B obtained physical meat quality (pH, drip loss, cooking loss, and water holding capacity) and meat chemical quality (moisture, protein, fat, and ash content) that were almost the same as the control (p > 0.05). By administering 24% CP-CH + 0% CP-152 (C) concentrate, there was a significant decrease in the physical and chemical quality of crossbreed landrace pigs (p < 0.05). Administration of CP-CH at 12%–24% did not affect total cholesterol, HDL, LDL, or blood TGs in landrace-crossbreed pigs. Conclusion: Giving 12% CP-CH (B) obtained meat quality (physical and chemical) almost the same as the control group. Treatment group C significantly reduced the physical and chemical quality of the meat. Administration of CP-CH at 12%–24% did not affect the blood profile of landrace crosses. [J Adv Vet Anim Res 2023; 10(4.000): 579-586]