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ISOLATION AND IDENTIFICATION OF STAPHYLOCOCCUS BACTERIA FROM FISH OF FRESH WATER AND ITS ANTIBIOTICS SENSITIVITY IN MOSUL CITY Texte intégral
2014
Haifaa Hussein Ali
Bacteriological examination was performed on 60 local healthy fish of fresh water include 30 Carp fish (Cyprinus carpio) and 30 Cat fish (Silurus glanis) with different weights from local retail fish markets at Mosul city, during the period from sept. 2011 - Sept. 2012. Swabs from skin and parts of muscles, livers, intestines incubated in brain heart infusion broth for 24 hours at 37 ̊ C (aerobic culture), a loopful from incubated broth were streaked on blood agar, milk agar, mannitol salt agar incubated plates at 37 ̊ C for 24 h, selected colonies were submitted to gram staining, morphological characteristics biochemical tests for Staphylococcus. The percentage of Staphylococcus isolation was 100% for all examined samples of fish. A total of 130 isolates from both two species of examined fish (62) isolates from Cyprinus carpio and (68) isolates from Silurus glanis , a five species of Staphylococcus S. saprophyticus (29% , 29.4%), S. epidermidis (21% , 22%), S. hyicus (17.75% , 17.7%), S. aureus (17.75% , 19.1), S. intermedius (14.5% , 11.8%) , were identified with different numbers and percentages for Cyprinus carpio and Silurus glanis respectively . While percentages of Staphylococcus isolates from skins (35.5% , 36.8%), muscles (17.7% , 20.6%), livers (25.8% , 25%), intestines (21% , 17.60%) from both species Cyprinus carpio and Silurus glanis respectively. Antibiotic sensitivity test result for six antibiotics (Ampicillin, Gentamicin, Chloramphenicol, Polymaxin, CO-Trimaxazol , Ciprofloxacin) were variable most species of Staphylococcus isolates were resistant to Ampicillin but sensitive to Ciprofloxacin.
Afficher plus [+] Moins [-]ACUTE TOXICITY STUDY OF NEW DAPSONE SCHIFF BASE DERIVATIVE IN LABORATORY RATS Texte intégral
2014
Wasfi A. Al-masoudi | Hassan T. hamed | Jalaa Al- ahamed
Dapsone (4,4-diaminodiphenylsulphon) is one of the types of sulfa drugs as an anti bacterial and is used particularly in the treatment of leprosy . The aim of present study to investigate in vivo toxic effect and find acute toxic doses ( LD50) of new dapsone Schiff base derivative compound ( 4-{[ 4(ethylideneamino)phenyl] sulfonyl} aniline, was evaluated in this study. The experiment was performed on 10-14-week old male and female albino rats ( 225± 25 g ) at the time of treatment. Its acute toxic dose being 0.58 g.
Afficher plus [+] Moins [-]ANTIMICROBIAL ACTIVITY OF FLAVONOID COMPOUND ISOLATED FROM INULA GREAVEOLENS L. PLANT ON SELECTED PATHOGENIC BACTERIA Texte intégral
2014
Ghosoon F. Al-Kanany | Sameerah Ahmed Zearah
The antibacterial activity of the flavonoid compound (B3) isolated from Inula greaveolens plant by column – chromatography was determined against several of clinical microbial isolates including Gram positive: ( Streptococcus spp. Staphylococcus aureus ) and isolates of Gram negative : ( Klebsiella pneumonia, Pseudomonas aeroginosa and Esherichia coli ) by using three concentrations of the extract (125 mg /ml , 250mg /ml ,500 mg /ml ).The results revealed that the falvonoid compound B3 has varying degrees of inhibition tested microorganisms. The cytotoxic activity of the falvonoid compound (B3) was determined against the fresh human red blood celles with several concentrations of the extract and the results shown that flavonoid compound did not had toxicity against the human red blood cells.
Afficher plus [+] Moins [-]ISOLATION AND IDENTIFICATION OF SALMONELLA SPP. FROM POULTRY FARMS BY USING DIFFERENT TECHNIQUES AND EVALUATION OF THEIR ANTIMICROBIAL SUSCEPTIBILITIES Texte intégral
2014
Ali A. AL-Iedani | Nael M. Oufi | Mohammed H. Khudor
This study aimed to isolate and identify Salmonella spp. from various sources of poultry farms by using four different techniques (conventional biochemical tests, API 20E system, serology and polymerase chain reaction) the total number of isolates was 44(9%). The Salmonellae including 4 species, S. gallinarum 9(1.85%), S. typhimurium 7(1.44%), S. newport 21(4.3%) and S. ohio 1(0.21%). The highest isolation rate was in first week of chicks life 18(25.7%), however, the highest isolation rate of salmonella was from liver 13(28.8%). There are similarity in identification rate of Salmonella spp. between API 20 E system and PCR assay using flic gene. In this study using PCR amplification of rfbsg and rfbsp genes in differentiation of Salmonella serovar gallinarum into S. gallinarum and S. pullorum biovars very useful. Results of antimicrobial susceptibility revealed high resistance of isolates against seven antibiotics arranged in descending from high to low resistance (Azithromycin, Florfenicol, Trimethoprime-sulphamethaxezole, Tetracycline, Ciprofloxacin, Ampicillin and Gentamycin).
Afficher plus [+] Moins [-]MAGNESIUM SULFATE, KETOROLAC, PROPOFOL, KETAMINE, AND XYLAZINE ANESTHETIC PROTOCOL IN RABBITS Texte intégral
2014
Ahmed A. AL- Mramudhi | haier A. Abid
The study aimed to evaluate the combination of Magnesium sulfate (Mg), ketorolac (Kr), Propofol (P), Ketamine (K), and Xylazine(X) anesthetic protocol in anesthesia and analgesia of rabbits. Twenty healthy male rabbits, weighing (1.300 0.200 kg) were used in the study. All rabbits were randomly assigned to four groups of five rabbits injected with the different protocols (G1(p10k50mg50 ), G2(p10k50kr10 ), G3(p10 k50 kr10mg50 ), and G4(p10 k50 kr10mg50x5)) of anesthesia intravenously in the marginal ear vein. The heart rate (HR), respiratory rate (RR), rectal temperature (RT) were taken before giving the drugs (Time 0 (control reading)), and then after 5,10,15,20,30,45,60,and 75 minutes of giving anesthesia. The induction time, duration of anesthesia, degree of analgesia, muscle relaxation and recovery time were recorded also. The anesthetic protocol in G3 (p10 k50 kr10mg50) is seen suitable for short operations (gives 24.2 minutes of surgical anesthesia), and the anesthetic protocol in G4 (p10 k50 kr10mg50x5) is seen suitable for long operations (gives 43.5 minutes of surgical anesthesia), and no signs of pain with the intravenously injection of propofol.
Afficher plus [+] Moins [-]THE EFFECT OF GENISTEIN ON SOME PRODUCTIVE AND BIOCHEMICAL BLOOD TRAITS OF QUAIL ( Coturnix coturnix japonica ) Texte intégral
2014
Majed H. AL-Asadi | Waleed Y. Kasim | Sajida A. Alshaheen
This study was conducted at commercial poultry farm in Aboalkassib from 9/3/2013 to 9/5/2013. A total of 60 day old quail chicks were randomly distributed to four groups , 15 birds for each treatments. Treatments were (1): control group , (2) : 100 ml genistein per 1 litter of drinking water, (3): 100 ml genistein plus 10 ml vitamin E per 1 litter of drinking water , and (4): 150 ml genistein plus 10 ml vitamin E per 1 litter of drinking water . T3 caused significantly (P
Afficher plus [+] Moins [-]INCIDENCE OF ILLEGAL SUBSTITUTION OF BEEF WITH INFERIOR-QUALITY ANIMAL SPECIES MEATS SOLD IN KERBALA Texte intégral
2014
Bahaa H.Muhammed | Hikmat Sahib Al-Nassir | Raeed A.Kassim
A surveillance study was conducted to determine the rates of mixing beef illegally with other selected animal species meats of inferior quality in Kerbala Governorate. Over an 8-month period ( September 2012 to May 2013), a total of 120 samples of meat products viz., 40 local minced meats, 40 local beef burger and 40 imported beef burger were purchased from Kerbala city and analyzed by Agar Gel Immuno-Diffusion test ( AGID) for detecting substitution of undesirable meat species. Meat extracts from camel, chicken and donkey were prepared. Rabbits were injected with meat extracts for preparation of hyper-immune sera, whereas, blood was collected to get the specific anti-sera. Positive result was recorded when precipitation line observed between the antibody and the corresponding meat extract. The incidence of adulteration was detected in 6 (5%) samples with camel meat and 23 ( 19.16%) samples with chicken meat, while none of the samples was found adulterated with donkey meat .Camel meat found in 4 (10%), 2 ( 5%) and 0 ( 0%) of local minced meat, local beef burger and imported beef burger respectively. However, chicken meat detected in 8 (20%), 10 (25%) and 5 ( 12.5%) of local minced meat, local beef burger and imported beef burger respectively. It was concluded that substitution of meat products, in which inferior quality meat is mixed into beef, has been existed in Kerbala G
Afficher plus [+] Moins [-]THE LAST POSITION OF OLD WORLD SCREW WORM IN IRAQ Texte intégral
2014
Muntasir Al-Ani | Khawla bedan Al-Jassim | Mushtaq Al-Helfi
Fourteen years of study from September 1996 to September 2010 included all province of Iraq. This study showed behavior and outbreak of old world screw worm from first recoded in Iraq with signal to its epidemiology. Investigation show proliferation and spread of fly in areas and the ways of reach this fly to in. many cases recorded at 60c and zeroC.erradication study where occur by using new method of spraying chemical pesticides and used ivermectin as injection s/c for animals as prevention. This technique improved that no cases were recorded in all town of Iraq especially in Basra which have big problem of infestation of old world screw worm in animals at 2004-2005. Study improve that were good professional staff of veterinarian in Iraq can eradication old world screw worm without using of sterile male fly technique of chrysomya bezziana.
Afficher plus [+] Moins [-]STUDY THE EFFECT OF USING PROBIOTIC (VETLACTOFLORUM) ON SOME OF BIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS OF BROILER CHICKENS Texte intégral
2014
E.A | Kapitonova | Glaskovich | A.A | Aamer R.A | Alaqaby | Losev
In the present study its used liquid preparation of probiotics "Vetlactoflorum-M" (dissolved in diluted milk) and "Vetlactoflorum-C" (dissolved in whey), and were studied its effect on some parameters such as total protein, globulin, albumin also serum bactericidal and lysozyme activity. One hundred fifty broilers chicken breed "Ross-308" used in one day old. They were divided into three groups of 50 chicks (control" un-supplemented with probiotic" , first treatment group received “Vetlactoflorum-C” and second treatment group received “Vetlactoflorum-M”). Blood samples were taken in 7, 14, 21, 28, 35 and 42 day-old. Laboratorial analyses were conducted in Vitebsk state academy of veterinary medicine in Belarus. Level of total protein in the 2nd group to the end of the study was higher than in control group to 8.1% (P
Afficher plus [+] Moins [-]SYNTHESIS, CHARECTERIZATION AND ANTIOXIDANT ACTIVITY OF BIS [4-(4'-HYDROXY-3'-METHOXY BENZYLIDENE AMINO PHENYL)] TELLURIDE Texte intégral
2014
Hiathem J. Kadhum | Mohammed A. Al-Dewan | Shaker A. S. N. AL-Jadaan
Bis[4-(4'-hydroxy-3'-methoxy benzylidene amino phenyl)] telluride (R2Te) was prepared from the reaction of two moles of 4-(4'-hydroxy-5'-methoxybenzylidene amino phenyl) mercuric chloride and one mole of TeBr 4 in dry dioxane to give diorganyl tellurium dibromide (R 2 TeBr2 ) which reduced by boiling ethanolic hydrazine hydrate to give the corresponding new telluride (R 2 Te). R2 Te inhibits nitrite induced methemoglobin formation in hemolysate. The time required to convert 50% of the available hemoglobin to methemoglobin was increased from 2.5 to 4, 5.5 and 10.5 minutes by the addition of (1.25, 5 and 20µM) of test compound (R 2 Te) respectively, and from 7 to 15, 29 and 54 minutes to convert all of the available hemoglobin to methemoglobin. It seems that R 2 Te failed to inhibit nitrite induced hemoglobin oxidation if added after the autocatalytic stage (5-10 minutes after nitrite addition). R 2 Te, a novel organotellurium compound, can inhibit nitrite induced methemoglobin formation in hemolysate in a dose dependent manner if added before the autocatalytic stage.
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